• IMMUNE NETWORK
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• 제7권3호
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• pp.133-140
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• 2007

Background: It is well established that cross talk between natural killer (NK) cells and myeloid dendritic cells (DC) leads to NK cell activation and DC maturation. In the present study, we investigated whether type 1-polarized DC (DC1) matured in the presence of IFN-${\gamma}$ and type 2-polarized DC (DC2) matured in the presence of PGE2 can differentially activate NK cells. Methods: In order to generate DC, plastic adherent monocytes were cultured in RPMI 1640 containing GM-CSF and IL-4. At day 6, maturation was induced by culturing the cells for 2 days with cytokines or PGE2 in the presence or absence of LPS. Each population of DC was cocultured with NK cells for 24 h. The antigen expression on DC was analyzed by flow cytometry and cytokine production in culture supernatant was measured by ELISA or a bioassay for TNF-${\alpha}$ determination. NK cell-mediated lysis was determined using a standard 4h chromium release assay. Results: DC2, unlike DC1, had weak, if any, ability to induce NK cell activation as measured by IFN-${\gamma}$ production and cytolytic activity. DC2 were weakly stimulated by activated NK cells compared to DC1. In addition, IFN-${\gamma}$-primed mature DC appeared to be most resistant to active NK cell-mediated lysis even at a high NK cell/DC ratio. On the other hand, PGE2-primed DC were less resistant to feedback regulation by NK cells than IFN-${\gamma}$-primed mature DC. Finally, we showed that the differential effect of two types of DC population on NK cell activity is not due to differences in their ability to form conjugates with NK cells. Conclusion: These results suggest that different combinations of inflammatory mediators differentially affect the effector function of DC and, as a result, the function of NK cells, eventually leading to distinct levels of activation in adaptive immunity.

• 한국지능시스템학회논문지
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• 제14권1호
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• pp.9-14
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• 2004

감정은 지능을 지닌 존재의 이성판단에 영향을 준다. 그래서 주변 환경정보에 의해 평가된 기본적이고 보편적인 감정을 로봇에 가미하면 더욱 인간과 가까운 지능 로봇이 될 것이다. 그러나 인간의 감정을 학습하기 위해서는 다양한 감각정보의 학습과 패턴 분류가 선행되어야 하고 이를 위해서 적합한 네트워크 구조가 요구된다. 신경망은 시스템의 특징을 추출하는데 매우 우수한 능력을 발휘하고 있다. 그러나 임시적 혼선현상과 지역 최소치에 수렴하는 단점이 있다. 그래서 복잡한 문제를 단순한 여러 개의 부분적인 문제로 나누어 해결하는 모듈라 설계방법이 관심의 대상이 되고 있다. 본 논문에서는 수많은 감정평가와 학습 데이터 패턴들을 학습하기 위해서 재결합과 재구성에 탁월한 성능을 지닌 Jacobs와 Jordan이 제안한 모듈라 네트워크와 상황의 재 표현이 가능하고 예측값과 모델링에 적합한 특징을 지닌 궤환 신경망을 결합하였다. 구성된 구조는 기존의 모듈라 네트워크의 학습결과와 비교 검토하였다.

• 마이크로전자및패키징학회지
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• 제6권2호
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• pp.37-43
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• 1999

대용량, 고속 정보처리가 요구되는 시스템의 모듈은 데이터 처리의 고속성 및 회로의 고집적이 가능한 MCM의 형태로 구현되어 ATM, GPS 및 PCS 등의 분야에 광범위하게 응용되고 있다. 3개의 칩으로 구성되고 2.48 Gbps의 데이터 처리용량을 가지는 ATM Switching 모듈을 기판 Size 48$\times$48mm2, Cu/PhotoBCB를 이용한 10 Multi-Layer 그리고 491 Pin PBGA 형태의 MCM을 개발하였다. MCM 개발을 위해 요구되는 기술로는 고속신호 특성구현을 위해 Interconnect Characterization을 통한 기판/ 패키지의 설계 파라미터 추출, 고밀도 MCM 에서의 방열처리 그리고 MCM 개발의 가장 난점중의 하나인 시험성 확보를 들 수 있다. ATM Switching MCM 개발을 위해 MCM-D 기판에서의 Interconnect Characterization을 통한 신호지연, 비아특성, 신호간섭(Cross-talk) 파라미터 등을 추출하였다. 고집적 구조에서 15.6Watt의 방열처리를 위해 열 해석을 진행하고 기판에 열 비아 1.108개를 형성하고 패키지 전체에 $85^{\circ}C$ 이하 유지조건의 방열처리를 하였다. 마지막으로 시험성 확보를 위해 미세 간격 프로빙을 통한 기판 검증 및 복잡한 패키지/어셈블리 공정검증을 위해 Boundary Scan Test(BST)를 적용하여 효과적이고 비용 절감형의 제품을 개발하였다.

• 한국항해항만학회:학술대회논문집
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• 한국항해항만학회 2006년도 International Symposium on GPS/GNSS Vol.2
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• pp.385-390
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• 2006

This paper examines the sampling and jitter specifications and considerations for Global Navigation Satellite Systems (GNSS) software receivers. Software radio (SWR) technologies are being used in the implementation of communication receivers in general and GNSS receivers in particular. With the advent of new GPS signals, and a range of new Galileo and GLONASS signals soon becoming available, GNSS is an application where SWR and software-defined radio (SDR) are likely to have an impact. The sampling process is critical for SWR receivers, where it occurs as close to the antenna as possible. One way to achieve this is by BandPass Sampling (BPS), which is an undersampling technique that exploits aliasing to perform downconversion. BPS enables removal of the IF stage in the radio receiver. The sampling frequency is a very important factor since it influences both receiver performance and implementation efficiency. However, the design of BPS can result in degradation of Signal-to-Noise Ratio (SNR) due to the out-of-band noise being aliased. Important to the specification of both the ADC and its clocking Phase- Locked Loop (PLL) is jitter. Contributing to the system jitter are the aperture jitter of the sample-and-hold switch at the input of ADC and the sampling-clock jitter. Aperture jitter effects have usually been modeled as additive noise, based on a sinusoidal input signal, and limits the achievable Signal-to-Noise Ratio (SNR). Jitter in the sampled signal has several sources: phase noise in the Voltage-Controlled Oscillator (VCO) within the sampling PLL, jitter introduced by variations in the period of the frequency divider used in the sampling PLL and cross-talk from the lock line running parallel to signal lines. Jitter in the sampling process directly acts to degrade the noise floor and selectivity of receiver. Choosing an appropriate VCO for a SWR system is not as simple as finding one with right oscillator frequency. Similarly, it is important to specify the right jitter performance for the ADC. In this paper, the allowable sampling frequencies are calculated and analyzed for the multiple frequency BPS software radio GNSS receivers. The SNR degradation due to jitter in a BPSK system is calculated and required jitter standard deviation allowable for each GNSS band of interest is evaluated. Furthermore, in this paper we have investigated the sources of jitter and a basic jitter budget is calculated that could assist in the design of multiple frequency SWR GNSS receivers. We examine different ADCs and PLLs available in the market and compare known performance with the calculated budget. The results obtained are therefore directly applicable to SWR GNSS receiver design.

• Toxicological Research
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• 제27권4호
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• pp.253-259
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• 2011

Transforming growth factor ${\beta}$ (TGF-${\beta}$) is involved in cellular processes including growth, differentiation, apoptosis, migration, and homeostasis. Generally, TGF-${\beta}$ is the inhibitor of cell cycle progression and plays a role in enhancing the antagonistic effects of many growth factors. Unlike the antiproliferative effect of TGF-${\beta}$, E2, an endogeneous estrogen, is stimulating cell proliferation in the estrogen-dependent organs, which are mediated via the estrogen receptors, $ER{\alpha}$ and $ER{\beta}$, and may be considered as a critical risk factor in tumorigenesis of hormone-responsive cancers. Previous researches reported the cross-talk between estrogen/$ER{\alpha}$ and TGF-${\beta}$ pathway. Especially, based on the E2-mediated inhibition of TGF-${\beta}$ signaling, we examined the inhibition effect of 4-tert-octylphenol (OP) and 4-nonylphenol (NP), which are well known xenoestrogens in endocrine disrupting chemicals (EDCs), on TGF-${\beta}$ signaling via semi-quantitative reverse-transcription PCR. The treatment of E2, OP, or NP resulted in the downregulation of TGF-${\beta}$ receptor2 (TGF-${\beta}$ R2) in TGF-${\beta}$ signaling pathway. However, the expression level of TGF-${\beta}1$ and TGF-${\beta}$ receptor1 (TGF-${\beta}$ R1) genes was not altered. On the other hand, E2, OP, or NP upregulated the expression of a cell-cycle regulating gene, c-myc, which is a oncogene and a downstream target gene of TGF-${\beta}$ signaling pathway. As a result of downregulation of TGF-${\beta}$ R2 and the upregulation of c-myc, E2, OP, or NP increased cell proliferation of BG-1 ovarian cancer cells. Taken together, these results suggest that E2 and these two EDCs may mediate cancer cell proliferation by inhibiting TGF-${\beta}$ signaling via the downregulation of TGF-${\beta}$ R2 and the upregulation of c-myc oncogene. In addition, it can be inferred that these EDCs have the possibility of tumorigenesis in estrogen-responsive organs by certainly representing estrogenic effect in inhibiting TGF-${\beta}$ signaling.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2019년도 춘계학술대회
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• pp.111-111
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• 2019

Glycyrrhizae radix is one of the most frequently prescribed ingredients in Oriental medicine, and G. radix extract has been shown to exert anti-cancer effects. However, the cellular and molecular mechanisms of apoptosis by G. radix are poorly defined. In the present study, it was examined the biochemical mechanisms of apoptosis by water extract of G. radix (WEGR) in human bladder T24 cancer cells. It was found that WEGR could inhibit the cell growth of T24 cells in a dose-dependent manner, which was associated with the induction of apoptotic cell death, as evidenced by the formation of apoptotic bodies, DNA fragmentation and increased populations of annexin-V positive cells. The induction of apoptotic cell death by WEGR was connected with an up-regulation of pro-apoptotic Bax protein expression and down-regulation of anti-apoptotic Bcl-2 and Bcl-xL proteins, and inhibition of apoptosis family proteins (XIAP, cIAP-1 and cIAP-2). In addition, apoptosis-inducing concentrations of WEGR induced the activation of caspase-9, an initiator caspase of the mitochondrial-mediated intrinsic pathway, and caspase-3, accompanied by proteolytic degradation of poly (ADP-ribose)-polymerase. WEGR also induced apoptosis via a death receptor-mediated extrinsic pathway by caspase-8 activation, resulting in the down-regulation of total Bid and suggesting the existence of cross-talk between the extrinsic and intrinsic pathways. Taken together, the present results suggest that WEGR may be a potential chemotherapeutic agent for the control of human bladder cancer cells.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2019년도 춘계학술대회
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• pp.113-113
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• 2019

Diallyl trisulfide (DATS) is an organic polysulfide compound found in garlic. Although certain studies have demonstrated that DATS possesses strong anti-inflammatory activity, the underlying molecular mechanisms remain largely unresolved. In this study, we examined whether DATS exerts anti-inflammatory activity and investigated the possible mechanisms. Our results indicated that DATS significantly suppressed the lipopolysaccharide (LPS)-induced release of nitric oxide (NO) and prostaglandin E2 by inhibiting inducible NO synthase and cyclooxygenase-2 expression at the transcriptional and post-transcriptional levels in RAW 264.7 macrophages. DATS also down-regulated Toll-like receptor 4 (TLR4) and myeloid differentiation factor 88 expression, and inhibited nuclear translocation of nuclear transcription factor-kappa B (NF-${\kappa}B$) in LPS-stimulated 264.7 macrophages. Furthermore, we found that these inhibitory effects of DATS were associated with the inhibition of chemokine receptor (CXCR4) and ligand (CXCL12) expression, and reactive oxygen species generation. Overall, the present data indicated that DATS had anti-inflammatory effects on LPS-activated macrophages, possibly via inhibiting the TLR4/NF-kB and/or chemokine signaling pathways, and DATS could be a potential drug therapy for inflammation and its associated diseases.

• 항공우주의학회지
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• 제31권1호
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• pp.24-30
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• 2021

Purpose: The purpose of this study is to consider ways to strengthen it through comparison and curriculum while recognizing the importance of global competencies in liberal arts education in universities. Methods: In order to explore ways to reinforce the sub-competence of global competencies, this study was conducted at a four-year university in Chungcheong-do for one year in 2019, such as 'Global Culture Talk', 'Global Travel', 'Global Nanta', and 'Making Global Friends'. Cases of comparison and application of educational programs were analyzed. The program was attended by the Department of Business Administration, Department of Aviation Service, Department of Design, Department of Manga Animation, Department of Broadcasting and Film, and foreign exchange students. The competency-centered curriculum not only has clear educational goals, but is also very advantageous in establishing a feedback system by measuring its performance. This study will assess the effectiveness of the education plan by diagnosing the change in competencies before and after the comparison and curriculum is operated. Results: The overall global competency has increased by 0.2 points compared to 2017. By subsector, it was found that the flexibility increased by 1.4 points. In the field of cross-cultural understanding, it rose 0.6 points, and in the field of global understanding, it rose 2.2 points, showing the largest increase in the sub-fields. Nevertheless, the field of global interest remains at a low level. This is considerably low compared to flexibility and ability to understand other cultures, and it is expected that measures for improvement should be continuously sought. Since the understanding of other cultures has already exceeded 60 points, it is expected that the global competency of the university will be strengthened if the level is consistently maintained and the emphasis is placed on enhancing flexibility and improving global understanding. Conclusion: The importance of strengthening global capabilities is steadily rising. Universities are also reorganizing the curriculum by analyzing the needs and satisfaction of education consumers to respond to this. The programs operated and analyzed in this study were also made as part of this effort. However, since there are various factors that affect global competency, it cannot be but admitted that it is not easy to gauge the change in competency with only a few programs and short-term efforts. However, if the efforts pursued by this study are accumulated and supplemented through feedback from a long-term perspective, it can be expected that there are not a lot of contributions to strengthening global competencies in liberal arts education.

• Tuberculosis and Respiratory Diseases
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• 제47권3호
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• pp.347-355
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• 1999

배경: Phospholipase C(PLC)는 세포의 성장, 분화, 변형(transformation)과 관련된 세포내 신호 전달과정에 중추적인 역할을 하는 효소이다. 이들 중 PLC-$\gamma$는 tyrosine kinase의 인산화에 의해 주로 활성화되는 데, 최근에 phosphatidic acid(PA), phosphatidy-linositol 3, 4, 5-trisphosphate($PIP_3$), tau 단백에 의한 활성화 기전이 밝혀진 바 있다. 특히 tau 단백은 bovine brain에서 arachidonic acid와 함께 PLC-$\gamma$를 활성화시키는 것으로 알려져 PLC-$\gamma$와 $PLA_2$ 사이의 cross-talk이 이루어질 가능성이 제시되고 있다. 최근 보고에 의하면 tau 단백과 같은 기전으로 PLC-${\gamma}1$ 활성화시키는 단백이 bovine lung에서 발견되었고, 이 활성화 단백을 정제 및 클론하여 AHNAK 단백임이 확인된 바 있다. 또한 PLC-${\gamma}1$이 유방암, 대장암, 위암 등에서 증가되어 있어 발암 과정과 연관되어 있음이 보고되어 왔으나 PLC-${\gamma}1$의 활성화 단백인 AHNAK 단백에 대해서는 질병과 관련되어 연구된 것이 아직 없는 실정이며 저자 등은 폐암 조직과 정상 폐조직에서 AHNAK 단백의 발현 양상을 연구하여 폐암의 발암과정에 AHNAK 단백이 관여함을 밝히고자 하였다. 대상 및 방법: 아주대학교 병원에 내원하여 폐암으로 수술을 받은 환자의 폐암 조직과 동일 환자의 정상 폐조직에서 AHNAK 단백의 발현양상을 western blot 분석과 면역조직화학적 염색방법을 통하여 조사하였다. 결과: 14예의 편평상피암 세포조직 중 8예 (57.1 %)와 14예의 선암 세포조직 모두에서 정상 대조군에 비해 AHNAK 단백의 발현이 증가하였고, 70 kDa~200kDa의 여러가지 분자량을 가지는 띠모양으로 나타났다. 면역조직화학적 염색에서도 정상 폐조직보다 폐암 조직내에서 강한 발색반응을 보였다. 결론: PLC-${\gamma}1$의 활성화 단백인 AHNAK 단백이 폐암 조직에서 정상 조직보다 과발현된 것은, AHNAK 단백이 PLC-${\gamma}1$을 활성화시켜 폐암의 발생 기전에 관여할 수 있음을 뒷받침한다고 하겠다.

• 한국지역지리학회지
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• 제20권2호
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• pp.163-175
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• 2014

본 연구는 필리핀 출신 결혼이주여성의 사회자본 특성을 근린지구 수준에서 설명하고자 한다. 연구의 주 대상은 한국의 대도시와 중소도시, 촌락 등에 거주하는 필리핀 출신 결혼이주여성들이며 그들이 주로 참여하는 근린공동 체- 학교, 성당공동체-에서 생산되는 사회자본에 대한 인식을 측정하여 개인적 특성과 공동체의 특성(결속력, 동질성 등)에 따른 사회자본의 특성에 대해 파악하였다. 연구 결과 학교공동체와 성당공동체에서 이주자의 개인적 특성 가운데 이주사회에서의 거주기간과 사회자본 간 관련성에서 차이를 보이고 있는데, 성당공동체는 거주기간과 사회자본 '관계'요소 간 정(+)적인 유의미한 결과가 도출되어 이주 후 기간이 증가할수록 성당공동체에서의 관계가 향상되는 것으로 나타났으며 학교공동체에서는 거주기간과 사회자본 '신뢰'요소가 부(-)적인 관계를 보이고 있어 거주기간이 길수록 신뢰가 오히려 감소하는 것으로 나타났다. 동질 성과 결속력, 사회자본의 양에서도 성당공동체가 우위를 점하고 있으며 특히 사회자본 '관계'는 공동체의 동질성과 더 밀접하며 '규범'은 공동체의 결속력과 밀접하다는 결과를 보이고 있다. 두 공동체에서 모두 의사결정과 가치추구 공유 및 규칙 준수 등의 '규범' 요소가 구성원 간의 '관계'요소와 유사하며, 만족도와 정서적 도움 및 제도에 대한 구성원들의 '신뢰'가 개인문제를 토론하고 참여하는 '정치'와 근접한다는 공통된 경향이 나타나고 있다. 연구과정에서 주목되는 것은, 초국가적 행태가 발생하는 회로로서의 성당공동체의 상징성으로, 성당공동체는 필리핀 결혼이주여성들의 고유한 집단적 문화와 개인적 욕구가 사회자본의 생산과 맞물리는 영역으로 확인되었다. 이는 필리핀 결혼이주여성들의 공동체와 사회자본의 특성에서 이주자로서의 개인적 특성과 함께 필리핀 결혼이주여성이 집단적으로 보유하는 family reunion 등의 사회 문화적 특질이 주는 영향 또한 간과할 수 없음을 잘 보여주고 있다. 이러한 부분은 민족적 정체성을 바탕으로 한 공동체성과 사회자본이 우리 사회와의 상호문화성을 공유하고 실천할 수 있는 주요 통로가 될 수도 있다는 점에서 의미 있으며 나아가 '민족 단위의 공동체와 그 사회자본 연구'는 향후 더 다양한 공동체를 대상으로 다층의 공간 스케일로 확장, 연구되어야 할 필요가 있다.