Proceedings of the Plant Resources Society of Korea Conference (한국자원식물학회:학술대회논문집)

The Plant Resources Society of Korea (한국자원식물학회)
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Anti-inflammatory effects of DATS via suppression of cross talk between the TLR4/NF-κB and CXCL12/CXCR4 pathways in LSP-stimulated RAW 264.7 macrophages

LSP로 유도된 RAW 264.7 대식세포에서 TLR4/NF-κB와 CXCL12/CXCR4 경로 억제를 통한 DATS의 항염증 효과

  • Jeong, Yong Tae (Animal & Plant Utilization Team, Nakdonggang National Institute of Biological Resources) ;
  • Hwang, Buyng Su (Animal & Plant Utilization Team, Nakdonggang National Institute of Biological Resources) ;
  • Kim, Min-Jin (Animal & Plant Utilization Team, Nakdonggang National Institute of Biological Resources) ;
  • Shin, Su Young (Animal & Plant Utilization Team, Nakdonggang National Institute of Biological Resources) ;
  • Oh, Young Taek (Animal & Plant Utilization Team, Nakdonggang National Institute of Biological Resources) ;
  • Kim, Chul Hwan (Animal & Plant Utilization Team, Nakdonggang National Institute of Biological Resources) ;
  • Eom, Jung Hye (Animal & Plant Utilization Team, Nakdonggang National Institute of Biological Resources) ;
  • Lee, Seung Young (Performance Management Division, Nakdonggang National Institute of Biological Resources) ;
  • Choi, Kyung Min (Performance Management Division, Nakdonggang National Institute of Biological Resources) ;
  • Jeong, Jin-Woo (Animal & Plant Utilization Team, Nakdonggang National Institute of Biological Resources) ;
  • Cho, Pyo Yun (Animal & Plant Utilization Team, Nakdonggang National Institute of Biological Resources)
  • 정용태 (국립낙동강생물자원관 동식물활용연구팀) ;
  • 황병수 (국립낙동강생물자원관 동식물활용연구팀) ;
  • 김민진 (국립낙동강생물자원관 동식물활용연구팀) ;
  • 신수영 (국립낙동강생물자원관 동식물활용연구팀) ;
  • 오영택 (국립낙동강생물자원관 동식물활용연구팀) ;
  • 김철환 (국립낙동강생물자원관 동식물활용연구팀) ;
  • 엄정혜 (국립낙동강생물자원관 동식물활용연구팀) ;
  • 이승영 (국립낙동강생물자원관 혁신성과부) ;
  • 최경민 (국립낙동강생물자원관 혁신성과부) ;
  • 정진우 (국립낙동강생물자원관 동식물활용연구팀) ;
  • 조표연 (국립낙동강생물자원관 동식물활용연구팀)
  • Published : 2019.04.25
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Abstract

Diallyl trisulfide (DATS) is an organic polysulfide compound found in garlic. Although certain studies have demonstrated that DATS possesses strong anti-inflammatory activity, the underlying molecular mechanisms remain largely unresolved. In this study, we examined whether DATS exerts anti-inflammatory activity and investigated the possible mechanisms. Our results indicated that DATS significantly suppressed the lipopolysaccharide (LPS)-induced release of nitric oxide (NO) and prostaglandin E2 by inhibiting inducible NO synthase and cyclooxygenase-2 expression at the transcriptional and post-transcriptional levels in RAW 264.7 macrophages. DATS also down-regulated Toll-like receptor 4 (TLR4) and myeloid differentiation factor 88 expression, and inhibited nuclear translocation of nuclear transcription factor-kappa B (NF-${\kappa}B$) in LPS-stimulated 264.7 macrophages. Furthermore, we found that these inhibitory effects of DATS were associated with the inhibition of chemokine receptor (CXCR4) and ligand (CXCL12) expression, and reactive oxygen species generation. Overall, the present data indicated that DATS had anti-inflammatory effects on LPS-activated macrophages, possibly via inhibiting the TLR4/NF-kB and/or chemokine signaling pathways, and DATS could be a potential drug therapy for inflammation and its associated diseases.

Keywords