• Title/Summary/Keyword: cross breeding

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Mapping of Quantitative Trait Loci Affecting Growth Traits in a Japanese Native Chicken Cross

  • Rikimaru, K.;Sasaki, O.;Koizumi, N.;Komatsu, M.;Suzuki, K.;Takahashi, Hideaki
    • Asian-Australasian Journal of Animal Sciences
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    • v.24 no.10
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    • pp.1329-1334
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    • 2011
  • The Hinai-dori is a breed of chicken native to Akita Prefecture, Japan. An $F_2$ resource population produced by crossing low- and high-growth lines of the Hinai-dori breed was analyzed to detect quantitative trait loci (QTL) for growth traits. Highly significant QTLs for body weight at 10 and 14 weeks of age and average daily gain between 4 and 10 weeks and between 10 and 14 weeks of age were accordingly mapped in a common region between ADL0198 and ABR0287 on chromosome 1 and between MCW0240 and ABR0622 on chromosome 4, respectively. A significant QTL for body weight at 4 weeks of age and a significant QTL for average daily gain between 0 and 4 weeks of age were mapped for the first time to the same region flanking ABR0204 and ABR0284 on chromosome 1. These QTLs are good candidates for application in the development of marker-assisted selection strategies for increasing growth efficiencies in the Hinai-dori breed and native breeds of chickens in Asia.

'Goldone', a Yellow - fleshed Kiwifruit Cultivar with Large Fruit Size

  • Kwack, Yong-Bum;Kim, Hong Lim;Lee, Jae Han;Chung, Kyeong Ho;Chae, Won Byoung
    • Horticultural Science & Technology
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    • v.35 no.1
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    • pp.142-146
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    • 2017
  • In Korea, kiwifruit is grown within a limited region on the southern coast where the climate is warm. Since the yellow - fleshed kiwifruit variety, 'Hort16A', first became commercially available, we have focused on breeding additional yellow - fleshed kiwifruits. Here, we describe the cultivar 'Goldone', which originated from 'Red Princess' and is characterized by red coloration around the fruit core. Conventional field crosses were performed in 2003, and permission for final release of 'Goldone' was obtained in 2011. This cultivar is very productive, with an average fruit weight of 129 g, which is 39% heavier than that of the cultivar 'Hayward'. 'Goldone' is harvested in late October, approximately 165 - 170 days after anthesis. In general, 'Goldone' has approximately eight flowers per fruiting shoot; these flowers must be thinned before blooming for commercial production. 'Goldone' was registered at the Korean Seed & Variety Service in 2014 for plant variety protection rights (grant no. 4835).

Breeding of Tobacco Mosaic Virus Resistant Flue-cured Tobacco Variety KF114 (담배 모자이크 바이러스(TMV)병에 저항성인 황색종 연초신품종 KF114 육성)

  • 정윤화;금완수;조명조;백기현;신승구;조수헌
    • Journal of the Korean Society of Tobacco Science
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    • v.17 no.1
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    • pp.41-48
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    • 1995
  • Tobacco mosaic virus (TMV), TMV-common strain, causes severe damage on flue-cured tobacco in Korea. As the results, breeding programs were conducted to transfer the TMV resistance from NC567 to other cultivars. Advantage of F1, hybrid may not lie only in their heterotic value but also in directly combining of valuable dominant traits, e.g. for disease resistance, either in pairs or as groups already assembled in the two parents. A new TMV resistant flue-cured tobacco variety KF114 was developed from a cross as maternal parent MSNC567 and NC82 at the Suwon Experiment Station. KF114 was more vigorous growth characteristics in early stage than that of NC82, standard variety in Korea, and other characters were similar to those of NC82. It os high resistance to TMV, but bacterial wilt(Pseudomonas solanacerum) and black shank (Phytophthora parasitica var. nicotianae) is comparable to NC82. It should adapt well to the flue-cured production area and can reduce premature flowering under unfavorable weather conditions. Yield of KF114 is 1% to 5% higher, and nearly equal in value per KG compared with NC82. This variety, F1 hybrid, met acceptable standards for chemical and physical characteristics of cured leaf and high ranked good smoking taste by panel members in Korea Ginseng and Tobacco Research Institute.

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Current status of tissue culture and genetic transformation systems in oilseed rape plants (Brassica napus L.) (유채 조직배양 및 형질전환 연구동향)

  • Lee, Sang-Il;Kim, Yun-Hye;Lee, Dong-Hee;Lee, Yu-Mi;Park, Seo-Jun;Kim, Jong-Bo
    • Journal of Plant Biotechnology
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    • v.37 no.4
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    • pp.379-387
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    • 2010
  • Oilseed rape (Brassica napus L.) is an important crop due to its high oil content in the seed. Recently, the demand for the improvement of crop for biodisel energy source is increased as oil prices in the world has increased dramatically. Until now, oilseed rape breeding was carried out by cross-hybridization between different varieties and related germplasms. However, like as many other crops, the application of tissue culture and gene transformation systems has been introduced into oilseed rape breeding program including the development of transgenic canola plants. In this study, we reviewed a history of tissue culture and genetic transformation research in oilseed rape plants and indicated some important aspects for the production of transgenic oilseed rape plants.

Inheritance Analysis of Giant Embryo Mutation Induced by T-DNA Insertion in Rice

  • Qin, Yang;Kim, Suk-Man;Park, Hee-Yeon;Sohn, Jae-Keun
    • Korean Journal of Breeding Science
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    • v.41 no.1
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    • pp.9-15
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    • 2009
  • Recently, giant embryonic rice and functional rice food are preferred by more consumers, which are attributed to the fact that the embryo has high concentrations of essential amino acids, fatty acids, and vitamins relative to other parts of rice grains. In this report, the heredity and stability of giant embryo mutations in successive generations were analyzed regarding a giant embryonic line, 'P47', induced by T-DNA insertion and a $F_2$ population from a cross between 'P47' and 'Junam'. The mutant lines with increases of 1.5, 1.7 and 1.8 times on embryo length, width and 100-embryo weight to those of the control showed stable inheritance across three generations. The continuous frequency distributions of embryo size in the $F_2$ population showed that the embryo size is a quantitative trait of polygene controlled. In addition, wide range of transgressive segregations of six traits affecting embryo size confirmed exchange of genetic materials and recombination between genes controlling embryo size. Five giant embryo mutant lines selected from the $F_2$ population will be used for artificial selection and improvement of giant embryonic varieties.

Breeding of Light yellow Color Spray Carnation 'Leo' for Cut Flower (연노랑색의 절화용 스프레이 카네이션 '레오' 육성)

  • Kwon, Oh-Keun;Shin, Hak Ki;Choi, Seong Roul;Joung, Hyang Young;Lee, Jung-Soo
    • FLOWER RESEARCH JOURNAL
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    • v.19 no.4
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    • pp.245-247
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    • 2011
  • A new spray carnation cultivar 'Leo' was released by the National Institute of Horticultural and Herbal Science in 2003. A cross was made between spray cultivar 'Ballantyne' and 'West peachy' in 1999. After investigation of the characteristics four years (from 2001 to 2003), it was finally selected in 2003. 'Leo' is a spray cultivar with light yellow color for a cut flower. The main characteristics are multi branching, long vase life and early flowering habit than 'Diamond'(control). It has medium resistance to Fusarium wilt and 9 days vase life.

A High Yield and Processing Potato Cultivar 'Taedong Valley'

  • Dhital, Shambhu P.;Lian, Yu J.;Hwang, Won N.;Lim, Hak T.
    • Korean Journal of Breeding Science
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    • v.42 no.3
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    • pp.207-211
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    • 2010
  • 'Taedong Valley' is a high yielding and processing potato cultivar, which is a clonal selection resulting from a cross between 'W870' and 'A88431-1'. It is a medium maturating cultivar with medium plant height and light green foliage. 'Taedong Valley' has profuse flowering habit and light purple flowers. Tubers are smooth, round, and with yellow skin, light yellow flesh, medium eye depth. Tubers have medium dormancy and good keeping quality. 'Taedong Valley' has stable yield under wide range of climatic conditions. It is resistant to common scab and potato virus Y, but moderately susceptible to late blight. It is also resistant to most of the disorders, particularly dehiscence, hollow heart and internal brown spots. This cultivar has high level of tuber uniformity and capable of yielding 43.6 t/ha which is about 9.0% higher than the control potato cultivar 'Atlantic' under optimum agronomical practices.

Characterization and Utilization of the Clubroot Resistant Genes in Chinese Cabbage (Brassica rapa L.)

  • Hatakeyama, Katsunori
    • 한국균학회소식:학술대회논문집
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    • 2015.05a
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    • pp.33-33
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    • 2015
  • Clubroot disease is the major threat to the production of Chinese cabbage (Brassica rapa L.) in Japan. Although the breeding of the clubtoot resistant (CR) cultivars is one of the most efficient ways to control this disease, the CR cultivars do not always have effects due to the breakdown of resistance. Therefore, it is necessary to develop the breeding strategy to accumulate multiple CR genes in a single cultivar effectively. We have identified two incomplete dominant CR loci, Crr1 and Crr2, which are originated from the European CR turnip Siloga. To investigate the effectiveness of marker-assisted selection (MAS) for CR breeding, the inbred line with Crr1 and Crr2 was crossed with parental lines of the existing CR $F_1$ cultivar of Chinese cabbage, followed by 5 times of MAS and backcrossing. The $F_1$ derived from a cross between the resulting parental lines improved the clubroot resistance as expected and had the same morphological characters as the original $F_1$ cultivar. We have shown that the Crr1 locus comprised two loci: Crr1a, which by itself conferred resistance to the mild isolate; and Crr1b, which had a minor effect, but was not required for Crr1a-mediated resistance. Further genetic analysis suggested that Crr1b was necessary to acquire resistance to the more virulent isolate in combination with Crr2. Molecular characterization of Crr1a encoding TIR-NB-LRR class of R protein revealed that there were at least 4 alleles in Japanese CR cultivars of Chinese cabbage. PCR analysis with Crr1a-specific markers demonstrated that the functional alleles were predicted to be present in European CR turnips, Debra and 77b besides Siloga, whereas rarely in Japanese CR cultivars, indicating that Crr1a is an useful source to improve the resistance of Chinese cabbage cultivars.

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Breeding of a Seedless Table Grape Cultivar 'Heukisul' (Vitis sp.) with High Quality (고품질 무핵 포도 품종 '흑이슬' 육성)

  • Park, Sung-Min
    • Horticultural Science & Technology
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    • v.29 no.5
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    • pp.507-509
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    • 2011
  • 'Heukisul' (3x) a cross made in 2000 between 'Kyoho' (4x, Vitis sp.) and 'Thompson Seedless' (2x, Vitis vinifera L.) in a seedless grape breeding program, was preliminarily selected in 2004. After regional adaptation testing under the name of 'KTS014' at two sites during 2004-2005, it was finally selected in 2006. 'Heukisul' showed low incidence of berry shattering, resulting in a good berry set compared to 'King Dela'. 'Heukisul' had budburst on April 23, flowering on in June 9, and fruit maturation in September 29 (almost the same as 'King Dela' at Chuncheon), and it was considered a midseason cultivar. The mean berry weight was 4.0 g, about 0.7 g heavier than 'King Dela', and mean soluble solids were $21.9\;^{\circ}Brix$, about $2.7\;^{\circ}Brix$ higher than 'King Dela'. The skin color was dark violet with abundant bloom and the flesh was very firm. Although the cluster was compact, it required no cluster thinning. Also the incidence of berry cracking was very low.

Utilization of Elite Korean Japonica Rice Varieties for Association Mapping of Heading Time, Culm Length, and Amylose and Protein Content

  • Mo, Youngjun;Jeong, Jong-Min;Kim, Bo-Kyeong;Kwon, Soon-Wook;Jeung, Ji-Ung
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.65 no.1
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    • pp.1-21
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    • 2020
  • Association mapping is widely used in rice and other crops to identify genes underlying important agronomic traits. Most association mapping studies use diversity panels comprising accessions with various geographical origins to exploit their wide genetic variation. While locally adapted breeding lines are rarely used in association mapping owing to limited genetic diversity, genes/alleles identified from elite germplasm are practically valuable as they can be directly utilized in breeding programs. In this study, we analyzed genetic diversity of 179 rice varieties (161 japonica and 18 Tongil-type) released in Korea from 1970 to 2006 using 192 microsatellite markers evenly distributed across the genome. The 161 japonica rice varieties were genetically very close to each other with limited diversity as they were developed mainly through elite-by-elite crosses to meet the specific local demands for high quality japonica rice in Korea. Despite the narrow genetic background, abundant phenotypic variation was observed in heading time, culm length, and amylose and protein content in the 161 japonica rice varieties. Using these varieties in association mapping, we identified six, seven, ten, and four loci significantly associated with heading time, culm length, and amylose and protein content, respectively. The sums of allelic effects of these loci showed highly significant positive correlation with the observed phenotypic values for each trait, indicating that the allelic variation at these loci can be useful when designing cross combinations and predicting progeny performance in local breeding programs.