• Journal of Plant Biotechnology
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• 제39권1호
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• pp.49-61
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• 2012

This review summarises the biology, discovery and applications of single nucleotide polymorphisms in complex polyploid crop genomes, with a focus on the important oilseed crop $Brassica$ $napus$. $Brassica$ $napus$ is an allotetraploid species, and along with soybean and oil palm is one of the top three most important oilseed crops globally. Current efforts are well underway to $de$ $novo$ assemble the $B.$ $napus$ genome, following the release of the related $B.$ $rapa$ 'A' genome last year. The next generation of genome sequencing, SNP discovery and analysis pipelines, and the associated challenges for this work in $B.$ $napus$, will be addressed. The biological applications of SNP technology for both evolutionary and molecular geneticists as well as plant breeders and industry are far-reaching, and will be invaluable to our understanding and advancement of the $Brassica$ crop species.

• 한국작물학회지
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• 제50권4호
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• pp.281-285
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• 2005

Pod dehiscence (PD), defined as the opening of pods along both the dorsal and ventral sutures, causes the seed to shatter in the field before harvesting and results in loss of seed yields. However, breeding for resistance to PD is difficult due to the complicated genetic behavior and environmental interaction. The objective of the present research was to analyze the genetic behavior of PD for improving the breeding efficiency of resistance to PD in soybean. PD after oven-drying the sampled pod at $40^{\circ}C$ for 24 hours was the most reliable to predict the degree of PD tested in the field. Keunolkong, a dehiscent parent, was crossed with non-dehiscent parents, Sinpaldalkong and Iksan 10. Using their $F_1\;and\;F_2$ seeds, PD was measured after oven drying the pod at $40^{\circ}C$ for 24 hours. The gene conferring PD behaved in different manners depending on the genetic populations. In the Keunolkong$\times$Sinpaldalkong population, PD seemed to be governed by single major recessive gene and minor genes, while several genes were probably involved in the resistance to pod dehiscence in the Keunolkong$\times$Iksan 10 population. Heritability for PD estimated in F2 population showed over $90\%$ in the two populations. High heritability of PD indicated that selection for resistance to PD should be effective in a breeding program. In addition, genetic mapping of quantitative locus (QTL) for PD in both populations may reveal that genes conferring PD are population-specific.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.116-116
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• 2017

Low-temperature is one of the environmental stress factors that affect plant growth and development and consequently limit crop productivity. The control of seed germination under low-temperature is organized by many genes which are called quantitative trait loci (QTLs). High germination rate for low-temperature is an important factor of growing rice. Previously, we identified a major QTL controlling low-temperature germinability in rice using 96 introgression lines (ILs) derived from a cross between Oryza rufipogon (Rufi) and the Korean japonica cultivar, 'Hwaseongbyeo (HS)'. A $BC_3F_7$ line (TR5) showed better low-temperature germinability than its recurrent parent. TR5 was crossed with HS to develop a segregating F2:3 populations for the target QTL. Six SSR markers polymorphic between HS and Rufi were used to screen and fine map the qLTG1. The qLTG1 on chromosome 1, which accounted for 55.5% of the total phenotypic variation, confirmed that Rufi allele enhanced the low-temperature germinability. Intervals between markers CRM16 and CRM15, four candidate genes were identified. The identified candidate genes, which are encoded by a protein of unknown function, showed their direct involvement on seed germination at low-temperature. To identify genes targeted by qLTG1, we investigated the expression profiles of these candidate genes and germination behavior of qLTG1 under different stress conditions and compared to HS, Rufi, and TR5 at $13{\pm}2^{\circ}C$ for 3 days after incubation. Furthermore, transgenic rice plants will also be developed to conduct a detailed investigation on low-temperature germinability. Hence, the QTL for low-temperature germinability would be useful in rice breeding programs especially in the development of lines possessing low-temperature germinability.

• 한국농공학회논문집
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• 제53권5호
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• pp.1-7
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• 2011

The objective of this study is to decide a spatial interpolation method on temperature data for the suitability analysis of garlic cultivation. In Korea, garlic is the second most cultivated condiment vegetable after red pepper. Nowadays warm-temperate garlic faces potential shift of its arable area according to warmer temperature in the Korean Peninsula, and the change can be drawn with the precise temperature map derived from interpolation on point-measured data. To find the preferable interpolation method in cases of germination and vegetative period of the garlic, different approaches were tested as follows: Inverse Distance Weighted (IDW), Spline, Ordinary Kriging (OK), and Universal Kriging (UK). As a result, IDW and UK show the lowest root mean square errors as for the germination and vegetative seasons, respectively. However, statistically significant difference was not revealed among the applied methods regarding the germinating period. Eventually this will contribute to mapping the suitable lands for the cultivation of warm-temperate garlic reasonably.

• 한국수자원학회:학술대회논문집
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• 한국수자원학회 2004년도 학술발표회
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• pp.1271-1275
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• 2004

The purpose of this study is to present a standardized scheme for providing agriculture-related information at various spatial resolutions of satellite images including Landsat +ETM, KOMPSAT-1 EOC, ASTER VNIR, and IKONOS panchromatic and multi-spectral images. The satellite images were interpreted especially for identifying agricultural areas, crop types, agricultural facilities and structures. The results were compared with the land cover/land use classification system suggested by Ministry of Construction & Transportation based on NGIS (National Geographic Information System) and Ministry of Environment based on satellite remote sensing data. As a result, high-resolution agricultural land cover map from IKONOS imageries was made out. The results by IKONOS image will be provided to KOMPSAT-2 project for agricultural application.

• 환경정책연구
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• 제10권1호
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• pp.49-70
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• 2011

농업 지역에서의 비점 오염원에 대한 기작과 발생을 해석하기 위해 많은 노력이 진행되고 있다. 또한 우리나라뿐 아니라 다른 나라에서도 수질 악화가 점점 심해지고 있는 것이 사실이다. 비점 오염물질은 직접유출과 기저유출로 분리되어 하천으로 이동된다. 보통 지하수는 지표수에 비여 수질이 양호하다고 여기지만 농업지역에서의 오염된 하천 주위의 지하수 오염은 심각할 수 있다. SWAT 모형은 용성 및 정확도가 높기 때문에 수문 및 수질 연구에 있어 국내외에서 널리 사용되고 있다. 하지만 SWAT 모형은 소유역별 수문학적 반응단위인 HRU를 이용하여 유역 내 수문 및 수질을 평가하는데, 소유역 내 HRU의 공간적인 정보를 표출하도록 되어 있지 않기 때문에 다양한 영농방법이 지표수 및 지하수에 미치는 영향을 공간적으로 분석하는 데 한계가 있다. 따라서 본 연구에서는 이러한 SWAT 모형의 단점을 개선할 수 있는 SWAT HRU Mapping module을 개발하였으며 이를 횡계 2리 유역에 적용하여 지하수 함양량 및 대수층 유입 $NO_3-N$ 부하량 및 농도를 분석하였다. 이에 따라 농경지에서 대수층으로 유입되는 $NO_3-N$ 부하량이 산림에 비해 상당히 많은 것으로 분석되었다. 또한 같은 밭 지역이라고 해도 재배되는 작물의 종류 및 시비량 등에 따라 대수층으로 유입되는 오염부하에는 상당히 다른 영향이 있을 수 있으며, 재배작물과 토양 특성에 따라 $NO_3-N$이 대수층으로 유입될 것으로 판단된다. 본 연구에서 개발된 SWAT HRU Mapping module은 유역에서의 기저유출을 통한 오염원의 시공간적 분석을 하는 데 매우 유용하게 활용될 수 있을 것이라 판단된다.

• 대한원격탐사학회지
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• 제35권5_1호
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• pp.637-647
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• 2019

작물의 스트레스를 탐지하는 것은 생산량 감소를 평가하는데 매우 중요하다. 광화학 반사 지수 (PRI)는 LUE의 원격 감지 지표로서 개발되었으며, 많은 연구자들에 의해 식생의 스트레스 탐지에 효과적으로 사용할 수 있음이 입증되었다. 그러나 원격탐사 영상에 기반한 PRI를 이용한 연구는 매우 드물다. 낮은 분광해상도로 인하여 드론 및 위성영상 기반의 PRI 모니터링이 어렵기 때문이다. 본 연구에서는 다중분광 센서 기반의 PRI를 산정하기 위하여 인접한 밴드를 이용한 융합 방법을 제안하였다. 제안한 기법을 초분광 및 다중분광 영상에 적용한 결과 산출된 PRI는 79%의 설명력을 나타내었으며, 지상고정형 센서의 관측값과도 유사한 변동 특성을 나타내었다. 따라서 밴드 융합에 의한 PRI는 작물 스트레스 평가에 적용이 가능할 것으로 판단된다.

• Genes and Genomics
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• 제40권12호
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• pp.1319-1329
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• 2018

SSRs were successfully isolated from the Perilla crop in our current study, and used to analyze Perilla accessions from East Asia. Analyses of the clear genetic diversity and relationship for Perilla crop still remain insufficient. In this study, 40 new simple sequence repeat (SSR) primer sets were developed from RNA sequences using transcriptome analysis. These new SSR markers were applied to analyze the diversity, relationships, and population structure among 35 accessions of the two cultivated types of Perilla crop and their weedy types. A total of 220 alleles were identified at all loci, with an average of 5.5 alleles per locus and a range between 2 and 10 alleles per locus. The MAF (major allele frequency) per locus varied from 0.229 to 0.943, with an average of 0.466. The average polymorphic information content (PIC) value was 0.603, ranging from 0.102 to 0.837. The genetic diversity (GD) ranged from 0.108 to 0.854, with an average of 0.654. Based on population structure analysis, all accessions were divided into three groups: Group I, Group II and the admixed group. This study demonstrated the utility of new SSR analysis for the study of genetic diversity and population structure among 35 Perilla accessions. The GD of each locus for accessions of cultivated var. frutescens, weedy var. frutescens, cultivated var. crispa, and weedy var. crispa were 0.415, 0.606, 0.308, and 0.480, respectively. Both weedy accessions exhibited higher GD and PIC values than their cultivated types in East Asia. The new SSR primers of Perilla species reported in this study may provide potential genetic markers for population genetics to enhance our understanding of the genetic diversity, genetic relationship and population structure of the cultivated and weedy types of P. frutescens in East Asia. In addition, new Perilla SSR primers developed from RNA-seq can be used in the future for cultivar identification, conservation of Perilla germplasm resources, genome mapping and tagging of important genes/QTLs for Perilla breeding programs.

• 한국환경농학회지
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• 제25권4호
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• pp.371-381
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• 2006

New proteomic techniques have been pioneered extensively in recent years, enabling the high-throughput and systematic analyses of cellular proteins in combination with bioinformatic tools. Furthermore, the development of such novel proteomic techniques facilitates the elucidation of the functions of proteins under stress or disease conditions, resulting in the discovery of biomarkers for responses to environmental stimuli. The ultimate objective of proteomics is targeted toward the entire proteome of life, subcellular localization biochemical activities, and the regulation thereof. Comprehensive analysis strategies of proteomics can be classified into three categories: (i) protein separation via 2-dimensional gel electrophoresis (2-DE) or liquid chromatography (LC), (ii) protein identification via either Edman sequencing or mass spectrometry (MS), and (iii) proteome quantitation. Currently, MS-based proteomics techniques have shifted from qualitative proteome analysis via 2-DE or 2D-LC coupled with off-line matrix assisted laser desorption ionization (MALDI) and on-line electrospray ionization (ESI) MS, respectively, toward quantitative proteome analysis. In vitro quantitative proteomic techniques include differential gel electrophoresis with fluorescence dyes. protein-labeling tagging with isotope-coded affinity tags, and peptide-labeling tagging with isobaric tags for relative and absolute quantitation. In addition, stable isotope-labeled amino acids can be in vivo labeled into live culture cells via metabolic incorporation. MS-based proteomics techniques extend to the detection of the phosphopeptide mapping of biologically crucial proteins, which ale associated with post-translational modification. These complementary proteomic techniques contribute to our current understanding of the manner in which life responds to differing environment.

• 대한원격탐사학회:학술대회논문집
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• 대한원격탐사학회 2007년도 Proceedings of ISRS 2007
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• pp.471-474
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• 2007

Eucalyptus plantations play a major role in the China's ecological, social, economic and other aspects and presently China is the second largest producer of Eucalyptus in the world next to Brazil. It was introduced as an ornamental tree during 1890 but later it became a commercial crop. During 1960s large number of Eucalyptus timber were used for railway sleepers and it was also used as shelter belt for rubber trees. It becomes one of the important national resources of commercial timber once the production reached to 5 million $m^{3}/yr$. Through Eucalyptus oil, it brought about 20% of foreign exchange. In the present study, it was aimed to estimate the Eucalyptus growing area in the southern Guangdong in China in terms of aerial extent and changes between 1991 and 2001 using Landsat TM and ETM+ data. Object based classification technique and subsequent temporal change detection analysis were followed to identify the changes between the periods. In the present study, the total area was divided into three classes viz., plantation area with trees, plantation area without trees and others. Object oriented classification was found to be more accurate in the present study. Overall increase of about 23.62 $km^{2}$ was noted between 1991 and 2001 in the plantation area. With reference to the present study area, the growth of Eucalyptus growing area was 7.4% in the 10 year periods. From this study it is clear that the area under Eucalyptus cultivation is growing considerably year by year in China. However, elaborate study must be conducted considering larger areas to accurately predict the growth of Eucalyptus growing areas.