• Title/Summary/Keyword: crop analysis

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Analysis of Sugars Content by Genotypes in 82 Radish (Raphanus sativus L.) (무 유전자원 82 계통의 유리당 함량 분석)

  • Seo, Mi-Suk;Chung, Joon-Hui;Park, Beom-Suk;Kim, Jung Sun
    • Korean Journal of Plant Resources
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    • v.31 no.5
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    • pp.453-465
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    • 2018
  • Radish (Raphanus sativus L.) is a species of the Brassicaceae family and an important root vegetable crop, produced worldwide. A total of 82 radish accessions with various morphological and physiological characteristics analyzed for total sugars content. These accessions includes five subspecies and classified as wild, wild-relative, traditional and improved cultivar. The four sugars, glucose, fructose, sucrose and maltose, showed various contents in 82 accessions. Total sugar content ranged from 5.64 to 46.68 mg/g and showed average 25.33 mg/g. Total sugar content was not statistically significant among the five subspecies, but individual sugar ratio varied. The wild, wild-relatives and traditional cultivars were not significantly among average total sugars content compared with improved cultivars. On the other hand, the wild and traditional cultivars were showed high ratio of individual sugars. These results could be valuable information for the development of new radish cultivars and regulation of sugars biosynthesis in radish.

Isolation and Functional Identification of BrDSR, a New Gene Related to Drought Tolerance Derived from Brassica rapa (배추 유래 신규 건조 저항성 관련 유전자, BrDSR의 분리 및 기능 검정)

  • Yu, Jae-Gyeong;Park, Young-Doo
    • Horticultural Science & Technology
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    • v.33 no.4
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    • pp.575-584
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    • 2015
  • Drought stress is a crucial environmental factor determining crop survival and productivity. The goal of this study was to clearly identify a new drought stress-tolerance gene in Brassica rapa. From KBGP-24K microarray data with the B. rapa ssp. pekinensis inbred line 'Chiifu' under drought stress treatment, a gene which was named BrDSR (B. rapa Drought Stress Resistance) was chosen among 738 drought-responsive unigenes. BrDSR function has yet to be determined, but its expression was induced over 6-fold by drought. To characterize BrDSR, the gene was isolated from B. rapa inbred line 'CT001' and found to contain a 438-bp open reading frame encoding a 145 amino acid protein. The full-length cDNA of BrDSR was used to construct an over-expression vector, 'pSL100'. Tobacco transformation was then conducted to analyze whether the BrDSR gene can increase drought tolerance in plants. The BrDSR expression level in T1 transgenic tobacco plants selected via PCR and DNA blot analyses was up to 2.6-fold higher than non-transgenic tobacco. Analysis of phenotype clearly showed that BrDSR-expressing tobacco plants exhibited more tolerance than wild type under 10 d drought stress. Taking all of these findings together, we expect that BrDSR functions effectively in plant growth and survival of drought stress conditions.

Analysis of the Climate inside Multi-span Plastic Greenhouses under Different Shade Strategies and Wind Regimes

  • He, Keshi;Chen, Dayue;Sun, Lijuan;Huang, Zhenyu;Liu, Zhenglu
    • Horticultural Science & Technology
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    • v.32 no.4
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    • pp.473-483
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    • 2014
  • In this work, the effects of shade combination, shade height and wind regime on greenhouse climate were quantified. A two-dimensional (2-D) computational fluid dynamics (CFD) model was developed based on an 11-span plastic greenhouse in eastern China for wind almost normal to the greenhouse orientation. The model was first validated with air temperature profiles measured in a compartmentalized greenhouse cultivated with mature lettuce (Lactuca sativa L., 'Yang Shan'). Next, the model was employed to investigate the effect of shade combinations on greenhouse microclimate patterns. Simulations showed similar airflow patterns in the greenhouse under different shade combinations. The temperature pattern was a consequence of convection and radiation transfer and was not significantly influenced by shade combination. The use of shade screens reduced air velocity by $0.02-0.20m{\cdot}s^{-1}$, lowered air temperature by $0.2-0.8^{\circ}C$ and raised the humidity level by 0.9-2.0% in the greenhouse. Moreover, it improved the interior climate homogeneity. The assessment of shade performance revealed that the external shade had good cooling and homogeneity performance and thus can be recommended. Furthermore, the effects of external shade height and wind regime on greenhouse climate parameters showed that external shade screens are suitable for installation within 1 m above roof level. They also demonstrated that, under external shade conditions, greenhouse temperature was reduced relative to unshaded conditions by $1.3^{\circ}C$ under a wind speed of $0.5m{\cdot}s^{-1}$, whereas it was reduced by merely $0.5^{\circ}C$ under a wind speed of $2.0m{\cdot}s^{-1}$. Therefore, external shading is more useful during periods of low wind speed.

Assessing Effects of Farming Activities on the Water Quality in a Small Agricultural Watershed (충남 고성천 유역의 하천 수질 평가를 이용한 유역단위 질소와 인 수지 분석)

  • Kim, Min-Kyeong;Roh, Kee-An;Park, Seong-Jin;Choi, Chul-Mann;Ko, Byong-Gu;Yun, Sun-Gang;Lee, Jong-Sik
    • Korean Journal of Environmental Agriculture
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    • v.28 no.1
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    • pp.32-37
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    • 2009
  • This study was conducted to assess the impact of agricultural land use on the water quality in mountain village farmlands. Two soil types, paddy and upland soil were analysed for their chemical properties. Although, pH and organic matter contents were not significantly different to each other, concentrations of available P and exchangeable K and Ca were much higher in upland soil compared with those in paddy soil. The results of water quality analysis indicated that, in Goseong-cheon watershed, the nutrients contents were much higher in downstream area than in upstream area. Particularly, the elevation of $COD_{Mn}$ and T-P were significant during rainy season. When paddy fields coexist with upland fields, SS, T-N, and T-P concentrations in paddy fields were lower than those of upland fields. This may indicate that paddy fields possess an water-purifying ability by absorbing nutrients. This effect may reduce excessive nutrients discharged into the neighboring streams which may cause eutrophication problem. The difference between inflow and outflow of nitrogen and phosphorus during the cultivation period were measured as -98.9 kg $ha^{-1}$ and -29.7 kg $ha^{-1}$, which means that nutrient outflow was higher than inflow. This may suggest that much nutrient was discharged during the crop harvest period.

Analysis of the Factors for Decrease of Rice Stripe Disease in Chungnam Province (충남지역의 벼 줄무늬잎마름병 발생감소 요인 분석)

  • Kim, Byung-Ryun;Jeong, Tae-Woo;Han, Kwang-Seop;Hahm, Soo-Sang;Kim, Young-Jin;Nam, Yun-Gyu;Choi, Hong-Soo;Kim, Jeong-Soo;Yu, Seung-Hun
    • Research in Plant Disease
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    • v.19 no.2
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    • pp.84-89
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    • 2013
  • The incidence factors of Rice stripe virus (RSV) were analyzed by studying the population density and the viruliferous insect rate (VIR) of small brown planthopper (SBPH), the incidence of stripe disease, alternate host, and susceptible cultivar in Chungnam Province. The population of overwintering SBPH had been decreasing, but the VIR of overwintering SBPH had not been differing for three years, 2008 to 2010. No RSV was detected in the natural host plants, such as short awn, annual bluegrass, and barley. In 2009, relatively large population of SBPH with the VIR of 5.4% migrated from China. However, there was no evidence relating of migration large amount of SBPH from China in 2008 and 2010. Also the infection rate of RSV in rice was less than 1% in these periods. The cultivation area of the susceptible varieties had steadily decreased from 41% to 19% from 2007 to 2009. Therefore, the reduction factors of rice stripe disease in Chungnam Province with higher influx of inoculum could be with an appropriate forecasting and chemical control, cultivation of resistant varieties, changes in the cropping system, and the low winter-spring temperature.

Comparative Analysis on the Invertebrate Biodiversity between Organic and Conventional Agriculture Fields (유기 및 관행농법 농경지의 무척추동물 생물다양성 비교)

  • Kim, Hoon;Seo, Jae-Hwi;Kim, Kyo-Jin;Choi, Ga-In;Jo, Young-Ju;Kim, Tae-Yeon;Moon, Myung-Jin
    • Korean Journal of Organic Agriculture
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    • v.25 no.4
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    • pp.875-901
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    • 2017
  • Although some skeptical views arguing that the impact of organic farming is quite limited to sustainable biodiversity, the organic agriculture has been generally accepted to have positive effects on various properties of soils not only on biophysical and ecological properties but also on biodiversity of the field. Therefore, this field investigation has been conducted to examine the effect of organic farming, relative to conventional agriculture, through a comparative investigations of the two systems for conserving local biodiversity of invertebrate animals. For this research, organic and conventional farming locations matched with both of a paddy field and a upland fields in Goesan-gun province (Chungcheongbuk-do, South Korea) were selected to compare the species richness of invertebrate biodiversity as follows: location OP (Organic Paddy field), location OU (Organic Upland field), location CP (Conventional Paddy field), and location CU (Conventional Upland field). Among 249 species collected, the numbers of species collected in the locations practicing organic farming were greater than the locations practicing conventional farming both in paddy fields (104 vs. 84 species) and upland fields (108 vs. 77 species). Changes in species richness can be shown in the selected locations depending on the agriculture system with organic (50 species) or conventional (44 species) farming systems in the paddy fields, similarly with organic (42 species) or conventional (35 species) farming in the upland fields, respectively. Our present research clearly shows that a wide range of taxa, including insects, spiders and other invertebrates, benefit from organic management through increases in abundance or species richness. It has been also identified that four orders (Hemiptera, Diptera, Hynemoptera, and Araneae) that are largely intrinsic to organic paddy field, and another three orders (Hemiptera, Coleoptera, and Araneae) that are particularly beneficial for organic crop field regime.

Analysis of junction site between T-DNA and plant genome in Lissorhoptrus oryzophilus resistance GM rice (벼물바구미 (Lissorhoptrus oryzophilus) 내충성 GM 벼에서 T-DNA와 게놈의 인접부위 분석)

  • Lee, Jin-Hyoung;Shin, Kong-Sik;Suh, Seok-Cheol;Rhim, Seong-Lyul;Lim, Myung-Ho;Woo, Hee-Jong;Qin, Yang;Kweon, Soon-Jong;Park, Soon-Ki
    • Journal of Plant Biotechnology
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    • v.41 no.3
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    • pp.127-133
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    • 2014
  • Four transgenic rice lines harboring insect-resistant gene cry3A showed ideal field performances characterized by high considerable resistance to rice water weevil (Lissorhoptrus oryzophilus Kuschel). In this study, we estimated the insertion number of foreign genes, and analyzed the flanking sequences of T-DNAs in rice genome. As a result, T-DNA of BT12R1 line was inserted in exon region of rice chromosome 10. Two copies of T-DNAs were inserted in line BT12R2. BT12R3 line was analyzed at only left border flanking sequence. BT12R4 line was confirmed one copy of foreign gene insertion at the position 24,516,607 ~ 24,516,636 of rice chromosome 5, accompanied by a deletion of 30 bp known genomic sequences. This intergenic position was confirmed none of expressed gene and any deletion/addition of T-DNA sequence. In conclusion, these molecular data of rice water weevil resistant Bt rice would be used to conduct the biosafety and environment risk assessment for GM crop commercialization.

A Smart Farm Environment Optimization and Yield Prediction Platform based on IoT and Deep Learning (IoT 및 딥 러닝 기반 스마트 팜 환경 최적화 및 수확량 예측 플랫폼)

  • Choi, Hokil;Ahn, Heuihak;Jeong, Yina;Lee, Byungkwan
    • The Journal of Korea Institute of Information, Electronics, and Communication Technology
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    • v.12 no.6
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    • pp.672-680
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    • 2019
  • This paper proposes "A Smart Farm Environment Optimization and Yield Prediction Platform based on IoT and Deep Learning" which gathers bio-sensor data from farms, diagnoses the diseases of growing crops, and predicts the year's harvest. The platform collects all the information currently available such as weather and soil microbes, optimizes the farm environment so that the crops can grow well, diagnoses the crop's diseases by using the leaves of the crops being grown on the farm, and predicts this year's harvest by using all the information on the farm. The result shows that the average accuracy of the AEOM is about 15% higher than that of the RF and about 8% higher than the GBD. Although data increases, the accuracy is reduced less than that of the RF or GBD. The linear regression shows that the slope of accuracy is -3.641E-4 for the ReLU, -4.0710E-4 for the Sigmoid, and -7.4534E-4 for the step function. Therefore, as the amount of test data increases, the ReLU is more accurate than the other two activation functions. This paper is a platform for managing the entire farm and, if introduced to actual farms, will greatly contribute to the development of smart farms in Korea.

Application and Development of 'Chestnut Management Standard Diagnostic Table' (밤나무 경영 표준진단표의 개발 및 적용)

  • Jeon, Jun-Heon;Yoo, Byoung-Il;Lee, Jung-Min;Ji, Dong-Hyun;Kim, Yeon-Tae;Kang, Kil-Nam
    • Journal of Korean Society of Forest Science
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    • v.101 no.4
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    • pp.695-702
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    • 2012
  • The chestnut management standard diagnostic table is developed and would be utilized in order that a manager raising chestnuts checks where the own level of management is and grasps current state of own for the purpose of planning aims and advancing toward a higher level. The developed 'Chestnut management standard diagnostic table' consisted of 3 first classified items, 19 second classified items and 2 product indicator items by the chestnut experts consultative meeting. A survey of 212 farmhouses in 4 major producing area was conducted. Except invalid survey of 53 farmers, 159 farmhouses interviewed were used in analysis. Total score was calculated with sum of each item's score. According to the survey results, average score is 68.0 and Buyeo received the highest score of 69.7 and Suncheon received the lowest score of 61.8 by regional groups. The higher the group in score, the better it is in output per hectare. But the property of 'the ratio of the best products in total products' does not show a statistical correlation. Generally the score of 'management-based evaluation indicator part' and 'management and sale capacity indicator part' in Suncheon was low because of many elderly people. In part of 'manufacturing technology indicator' as Environmentally-Friendly production is progressed in over 70% of four regions, when comes to a disease and insect pest control there are rarely farmhouses having a way of crop dusting.

A Study on Performance Improvement of Fruit Vegetables Automatic Grafting System (과채류 접목시스템 개선 연구)

  • Kang, Dong Hyeon;Lee, Si Young;Kim, Jong Koo;Park, Min Jung;Son, Jin Kwan;Yun, Sung-Wook;An, Se Woong;Jung, In Kyu
    • Journal of Bio-Environment Control
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    • v.26 no.3
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    • pp.215-220
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    • 2017
  • This study was conducted to improve the insufficiency of fruit vegetable grafting system developed by National Institute of Agricultural Sciences, Rural Development Administration. When the rotary blade cut the stem of scions and rootstocks, the grafting failure at curved cutting surfaces happened. The cutting depth of a tomato seedling by a rotated cutter was calculated 0.11 mm even when the cutting arm length and the maximum stem diameter were 50 mm and 5 mm, respectively. Mathematical analysis and high-speed photography showed that there was no problem by cutting in straight the stem of scions and rootstocks. The compression test of seedling stems to design the optimal shape of gripper showed that stems were not completely restored when they were compressed above 0.8 mm and 0.6 mm in case of rootstocks and scion, respectively. This study found that the bending angle of stem of tomato seedlings at the grafting period was 10 degree on average. The optimal gripper finger was the edge finger type which could be precisely set center point by adjusting the distance between fingers. In addition, it was found that most of seedling could be grasped without damage when the finger-to-finger distances is set to 2.5 mm for scion and 3.0 mm for rootstocks and finger are coated by 1 mm-thick flexible material.