Browse > Article
http://dx.doi.org/10.5010/JPB.2014.41.3.127

Analysis of junction site between T-DNA and plant genome in Lissorhoptrus oryzophilus resistance GM rice  

Lee, Jin-Hyoung (Department of Agricultural Biotechnology, National Academy of Agricultural Science, Rural Development Administration)
Shin, Kong-Sik (Department of Agricultural Biotechnology, National Academy of Agricultural Science, Rural Development Administration)
Suh, Seok-Cheol (Department of Agricultural Biotechnology, National Academy of Agricultural Science, Rural Development Administration)
Rhim, Seong-Lyul (Inwoo Cooperation Ltd.)
Lim, Myung-Ho (Department of Agricultural Biotechnology, National Academy of Agricultural Science, Rural Development Administration)
Woo, Hee-Jong (Department of Agricultural Biotechnology, National Academy of Agricultural Science, Rural Development Administration)
Qin, Yang (Department of Agricultural Biotechnology, National Academy of Agricultural Science, Rural Development Administration)
Kweon, Soon-Jong (Department of Agricultural Biotechnology, National Academy of Agricultural Science, Rural Development Administration)
Park, Soon-Ki (Department of Agricultural Biotechnology, National Academy of Agricultural Science, Rural Development Administration)
Publication Information
Journal of Plant Biotechnology / v.41, no.3, 2014 , pp. 127-133 More about this Journal
Abstract
Four transgenic rice lines harboring insect-resistant gene cry3A showed ideal field performances characterized by high considerable resistance to rice water weevil (Lissorhoptrus oryzophilus Kuschel). In this study, we estimated the insertion number of foreign genes, and analyzed the flanking sequences of T-DNAs in rice genome. As a result, T-DNA of BT12R1 line was inserted in exon region of rice chromosome 10. Two copies of T-DNAs were inserted in line BT12R2. BT12R3 line was analyzed at only left border flanking sequence. BT12R4 line was confirmed one copy of foreign gene insertion at the position 24,516,607 ~ 24,516,636 of rice chromosome 5, accompanied by a deletion of 30 bp known genomic sequences. This intergenic position was confirmed none of expressed gene and any deletion/addition of T-DNA sequence. In conclusion, these molecular data of rice water weevil resistant Bt rice would be used to conduct the biosafety and environment risk assessment for GM crop commercialization.
Keywords
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Tohidfar M, Zare N, Jouzani GS, Eftekhari SM (2013) Agrobacterium-mediated transformation of alfalfa (Medicago sativa) using a synthetic cry3a gene to enhance resistance against alfalfa weevil. Plant Cell Tiss Organ Cult 113:227-235   DOI
2 Stout MJ, Riggio MR, Zou L, Roberts R (2002) Flooding influences ovipositional and feeding behavior of the rice water weevil (Coleoptera: Curculionidae). J Econ Entomol 95:715-21   DOI
3 Stout MJ, Frey MJ (2007) Evaluation of thiamethoxam as a seed treatment against the rice water weevil. Ann Res Rpt Rice Res Stn LSU Agric Ctr 99:299-300
4 Stout MJ, Hummel NA, Frey MJ, Rice WC (2011) The Impact of Planting Date on Management of the Rice Water Weevil in Louisiana Rice. J Econ Entomol 5:1-9
5 Uhm KB, Lee YI, Kim YH, Choi KM, You KS (1989) Studies on the future dispersion of the rice water weevil, Lissorhoptrus oryzophilus, in korea. Research Report RDA 31:23-28
6 Webb JL (1914) Notes on the rice water weevil (Lissorhoptrus simplex Say). J Econ Entomol 7:432-438   DOI
7 Zhai BP, Cheng JA, Huang EY, Shang HW, Zheng XH, Wu J, Fang YJ, Xia WQ, Lu XJ(1997) Population dynamics of rice water weevil in double rice cropping area of Zhejiang Province, China. Sci Agric Sin 30(6):23-29
8 Zou L, Stout MJ, Dunand RT (2004) The effects of feeding by the rice water weevil, Lissorhoptrus oryzophilus Kuschel, on the growth and yield components of rice, Oryza sativa. Agric For Entomol 6:47-53   DOI
9 Zhou Z, Pang J, Guo W, Zhong N, Tian Y, Xia G, Wu J (2012) Evaluation of the resistance of transgenic potato plants expressing various levels of Cry3A against the Colorado potato beetle (Leptinotarsa decemlineata Say) in the laboratory and field. Pest Manag Sci 68(12):1595-604   DOI
10 Bugert P, Decker S, Kluter H (2001) Improved PCR-walking for large-scale isolation of plant T-DNA borders. BioTechniques 30:496-504
11 Espino L, Way MO, Pearson R, Nunez M (2009) Effect of planting date on Lissorhoptrus oryzophilus (Coleoptera: Curculionidae) density-yield relationship on rice in southeastern Texas. J Econ Entomol 102:1536-1545   DOI
12 Hiei Y, Ohta S, Komari T, Kumashiro T (1994) Efficient transformation of rice (Oryza sativa L.) mediated by agrobacterium and sequence analysis of the boundaries of the T-DNA. Plant J 6:271-282   DOI   ScienceOn
13 De Buck S, De Wilde C, Van Montagu M, Depicker A (2000) T-DNA vector backbone sequences are frequently integrated into the genome of transgenic plants obtained by Agrobacterium mediated transformation. Mol Breed 6:459-468   DOI   ScienceOn
14 Japan Plant Protection Association (1986) Control of the Rice Water Weevil in Japan (in Japanese)
15 Gonzalez RG, Sanchez DS, Guerra ZZ, Campos JM, Quesada AL, Valdivia RM, Arencibia AD, Bravo KQ, Caligari PDS (2008) Efficient regeneration and Agrobacterium tumefaciens mediated transformation of recalcitrant sweet potato (Ipomoea batatas L.) cultivars. Asia Pac J Mo. Biol Biotechnol 16 16:25-33
16 Hibbard BE, Clark TL, Ellersieck MR, Meihls LN, El Khishen AA, Kaster V, Steiner HY, Kurtz R (2010) Mortality of western corn rootworm larvae on MIR604 transgenic maize roots: field survivorship has no significant impact on survivorship of F1 progeny on MIR604. J Econ Entomol 103:2187-2196   DOI
17 Hoefte H, Whiteley HR (1989) Insecticidal Crystal proteins of Bacillus thuringiensis. Microbiol Rev 53:242-255
18 Jouzani GS, Goldenkova IV, Piruzian ES (2008) Expression of hybrid cry3aM-licBM2 genes in transgenic potatoes (Solanum tuberusom). Plant Cell Tissue Organ Cult 92:321-325   DOI
19 Kim JH, Lee S (2007) Analysis of junction between T-DNA and plant genome in transgenic Arabidopsis thaliana. J of plant biology 50:455-460   DOI
20 Kim SR, Lee J, Jun SH, Park S, Kang HG, Kwon S, Ahn GH (2003) Transgene structures in T-DNA-inserted rice plants. Plant Mol Biol 52:761-773   DOI   ScienceOn
21 Krieg, Aloisius, Huger, Alois S (1989) Protein toxin from Bacillus thuringiensis which is toxic to coleopteran. Patent US 488 9918
22 Lee JH, Shin KS, suh SC, Rhim SL, Lee TH, Lim MH, Woo HJ, Qin Y, Cho HS (2013) CryIIIA toxin gene expression in transgenic rice confers resistance to rice water weevil. Plant Cell Tiss Organ Cult 115:243-252.   DOI
23 Rhim SL, Cho HW, Kim BD, Schnetter W, Geider K (1995) Development of insect resistance in tomato plants expressing the ${\delta}$-endotoxin gene of Bacillus thuringiensis subsp. tenebrionis. Molecular Breeding 1:229-236.   DOI
24 Kononov ME, Bassuner B, Gelvin SB (1997) Integration of T-DNA vector 'backbone' sequence into the tobacco genome: evidence for multiple complex patterns of integration. Plant J 11:945-957   DOI   ScienceOn
25 Kuraya Y, Ohta S, Fukuda M, Hiei Y, Murai N, Hamada K, Ueki J, Imaseki H, Komari T (2004) Suppression of transfer of non-T-DNA 'vector backbone' sequences by multiple left border repeats in vectors for transformation of higher plants mediated by Agrobacterium tumefaciens. Mol Breed 14:309-320   DOI
26 Lange WH, Grigarick AA (1959) Rice water weevil, beetle pest in rice growing areas of southern states discovered in California. Calif. Agric 13(8):10-11.
27 Rhim SL, Kim IG, Jin TE, Lee JH, Kuo CI, Suh SC, Huang LC (2004) Transformation of Citrus with Coleopteran Specific ${\delta}$-endotoxin Gene from Bacillus thuringiensis ssp. Tenebrionis. J Plant Biotechnol 6:21-24   과학기술학회마을
28 Saito T, Hirai K, Way MO (2005) The rice water weevil, Lissorhoptrus oryzophilus Kuschel (Coleoptera: Curculionidae). Appl Entomol Zool 40:31-39   DOI   ScienceOn
29 Stahl R, Horvath H, van Fleet J, Voetz M, von Wettstein D, Wolf N (2002) T-DNA integration into the barley genome from single and double cassette vectors. Proc Natl Acad Sci USA 99:2146-2151   DOI
30 Shang H, Stout MJ, Zhang Z, Cheng J (2004) Rice water weevil (Coleoptera: Curculionidae) population dynamics in Louisiana. J Entomol 39:623-42