• Journal of the Korean Geotechnical Society
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• v.26 no.6
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• pp.57-70
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• 2010

Recent earthquakes have demonstrated that the tunnels, which were once considered to be highly resistant to earthquakes, are susceptible to substantial damage under severe seismic loading. Among various modes of deformation under an earthquake loading, the response of the tunnel in the transverse direction is known to be the critical mode. This paper investigates the seismic response of the tunnel in the transverse direction using the method of seismic displacement, which is a type of pseudo-static analysis. Firstly, the methods of calculating the ground deformation are compared. It is shown that the single and double cosine may not provide an accurate estimation of the ground deformation, and that a one-dimensional site response analysis needs to be performed for a more reliable evaluation. Secondly, the tunnel responses are calculated using the simplified, analytical, and numerical solutions. It is demonstrated that the simplified method provides poor estimates of the tunnel response ground deformation. The analytical solution is shown to be effective in modeling circular tunnels in uniform ground, but has serious limitation in modeling tunnel response in non-uniform ground. Numerical analyses are shown to be applicable to all cases, and give the most accurate estimates of the tunnel response. It is also demonstrated that the linear solutions can be so conservative that the soil nonlinearity needs to be accounted for more accurate evaluation of the tunnel response.

• The Journal of Korean Obstetrics and Gynecology
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• v.22 no.1
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• pp.1-14
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• 2009

Purpose: Oxidative stress was thought to play a critical role in neurodegenerative disease. Many in vivo and in vitro reports explained the possible pathway of human aging. But in therapeutic aspects, there was no clear answers to prevent aging associated with neural diseases. In this study, we investigated the antioxidant and neuroprotective effects of the Insamyangyung-tang (IYT). Methods: To estimate the antioxidant effects, we carried out 1.1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging assay, 2,2'-azinobis-(3- ethylbenzothiazoline-6- sulfonic acid (ABTS) radical cation decolorization assay, and measurement of total polyphenolic content. To evaluate neuroprotective effect of IYT in vitro. We performed thiazolyl blue tetrazolium bromide (MTT) assay, reactive oxygen species (ROS) creation in SH-SY5Y. Tyrosine hydroxylase (TH) immunocytochemistry, nitric oxide (NO) assay, and TNF-${\alpha}$ assay in primary rat mesencephalic dopaminergic neurons. Results: The $IC_{50}$ values were $571.6{\mu}g/m{\ell}$ and $202.3{\mu}g/m{\ell}$ in DPPH and ABTS assay respectively. Total polyphenolic content was 1.05%. In SH-SY5Y culture, IYT significantly increased the decreased cell viability by 6-OHDA at the concentrations of $10{\mu}g/m{\ell}$ in pre-treatment group, $10-100{\mu}g/m{\ell}$ in post-treatment group, and $100{\mu}g/m{\ell}$ in co-treatment group. The production of ROS induced by 6-OHDA was significantly inhibited in IYT treated group. In mesencephalic dopaminergic cell culture, the IYT group reduced the dopaminergic cell loss against 6-OHDA toxicity and the production of No and TNF-${\alpha}$ at the concentration of $0.2{\mu}g/m{\ell}$. Conclusion: These results showed that IYT has antioxidant and neuroprotectctive effects in the dopaminergic cells through decreasing the production of ROS, NO and TNF-${\alpha}$ which can cause many neurodegenerative changes in brain cell.

• The Journal of Korean Obstetrics and Gynecology
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• v.22 no.1
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• pp.15-30
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• 2009

Purpose: In this rapidly aging society, the research and development of traditional oriental medicine treatment is one of the critical factors to protect the increasing neuro-degenerative disorders. In this study, we wanted to verify the effect of Yukul-tang (YUT) on neuro-degenerative disease model by assessing the antioxidant and anti-inflammation effects. Methods: To assess the antioxidant effects of YUT, we carried out DPPH radical and ABTS radical scavenging assays and determined the total polyphenolic contents in YUT. To evaluate the neuro-protective effects of YUT, we performed the MTT and ROS assays and TH immunohistochemistry, NO and TNF-${\alpha}$ assays in SH-SY5Y or mesencephalic dopaminergic neurons damaged by 6-OHDA. Results: The treatment of YUT showed eliminating effects on DPPH radical and ABTS radical. it showed deterring effects on ROS, NO and TNF-${\alpha}$ and protecting effects on TH-positive cell in SH-SY5Y cells or mesencephalic dopaminergic neurons. Especially in the case of the treatment of YUT with 0.2ug/mL + 6-OHDA 10uM, the protective effect on dopaminergic neurons was most outstanding. Conclusion: In this study, we have demonstrated that YUT has an antioxidant effect and a neuro-protective effect on neuro-degenerative disease model caused by neurotoxin such as 6-OHDA. The results of our present study suggest that YUT can be useful agent to prevent and to treat neuro-degenerative diseases.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.26 no.4A
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• pp.647-657
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• 2006

Probabilistic Risk Assessment considering statistically random variables is performed for the preliminary design of a Cable Stayed Bridge, which is Prestressed Concrete Bridge consisted of cable and plate girders, based on the method of Working Stress Design and Strength Design. Component reliabilities of cables and girders have been evaluated using the response surface of the design variables at the selected critical sections based on the maximum shear, positive and negative moment locations. Response Surface Method (RSM) is successfully applied for reliability analyses for this relatively small probability of failure of the complex structure, which is hard to obtain through Monte-Carlo Simulations. or through First Order Second Moment Method that can not easily calculate the derivative terms of implicit limit state functions. For the analysis of system reliability, parallel resistance system consisting of cables and plate girder is changed into series connection system and the result of system reliability of total structure is presented. As a system reliability, the upper and lower probabilities of failure for the structural system have been evaluated and compared with the suggested prediction method for the combination of failure modes. The suggested prediction method for the combination of failure modes reveals the unexpected combinations of element failures in significantly reduced time and efforts compared with the previous permutation method or system reliability analysis method, which calculates upper and lower bound failure probabilities.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.29 no.6C
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• pp.251-258
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• 2009

In this study, the optimum design conditions for embankment construction on soft clay layer improved by soil compaction pile (SCP) are discussed by comparing the practical design method to the reliability design which is based on the loss function and advanced first order second moment (AFOSM) method. The results are summarized as follows; 1) the relationship between safety factor and failure probability becomes heavy exponentially, failure probability decreases rapidly till 1% approximately until safety factor is smaller than 1.2 and after then, failure probability decrease gradually along the increase of the safety factor. The design safety factor of 1.2 may be the critical value that has been established on considering both relationships appropriately, 2) the safety factor of 1.15 at the minimum expected total cost is a little smaller than the design safety factor of 1.2 and the failure probability is about 1%, 3) the sensitivities of the ratio of stress share and the internal friction angle of sand is larger than the variables related the undrained shear strength of soft layer. This result means that the distribution characteristic of n and ${\phi}$ influences on the stability analysis considerably and they should be considered necessarily on stability analysis of embankment on soft layer improved by SCP, 4) new failure points of the input variables at the design safety factor of 1.2(below failure probability of 0.1~0.3%) is far 1~2 times of standard deviation from the initial design values of themselves.

• Journal of the Korean neurological association
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• v.35 no.1
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• pp.8-15
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• 2017

Near-infrared spectroscopy (NIRS), a noninvasive optical method, utilizes the characteristic absorption spectra of hemoglobin in the near-infrared range to provide information on cerebral hemodynamic changes in various clinical situations. NIRS monitoring have been used mainly to detect reduced perfusion of the brain during orthostatic stress for three common forms of orthostatic intolerance (OI); orthostatic hypotension, neurally mediated syncope, and postural orthostatic tachycardia syndrome. Autonomic function testing is an important diagnostic test to assess their autonomic nervous systems for patients with symptom of OI. However, these techniques cannot measure dynamic changes in cerebral blood flow. There are many experimentations about study of NIRS to reveal the pathophysiology of patients with OI. Research using NIRS in other neurologic diseases (stroke, epilepsy and migraine) are ongoing. NIRS have been experimentally used in all stages of stroke and may complement the established diagnostic and monitoring tools. NIRS also provide pathophysiological approach during rehabilitation and secondary prevention of stroke. The hemodynamic response to seizure has long been a topic for discussion in association with the neuronal damage resulting from convulsion. One critical issue when unpredictable events are to be detected is how continuous NIRS data are analyzed. Besides, NIRS studies targeting pathophysiological aspects of migraine may contribute to a deeper understanding of mechanisms relating to aura of migraine. NIRS monitoring may play an important role to trend regional hemodynamic distribution of flow in real time and also highlights the pathophysiology and management of not only patients with OI symptoms but also those with various neurologic diseases.

• Journal of The Korean Society of Integrative Medicine
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• v.12 no.1
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• pp.1-10
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• 2024

Purpose: Lycopene is abundantly contained in Tomatoes and is known for diverse biological activities such as antioxidant, anti-inflammatory, and anticancer effects. In this study, the antioxidative potential of lycopene was investigated through the induction of hemeoxygenase (HO)-1 by nuclear factor-erythroid 2 p45-related factor2 (Nrf2) and upstream signaling molecules, mitogen-activated protein kinase (MAPK) and phosphoinositide 3-kinase (PI3K)/Aktin RAW 264.7 cells. Methods: The antioxidative potential of lycopene against oxidative stress and its molecular mechanisms were determined by the cell viability assay, intracellular reactive oxygen species (ROS) formation assay, and Western blot analysis in RAW 264.7 cells. Results: Lycopene treatment significantly attenuated tert-butyl hydroperoxide (t-BHP) induced intracellular ROS formation in a dose-dependent manner without any cytotoxicity. In addition, 50 µM of lycopene for 6 h treatment induced potent HO-1 expression and its transcription factor, Nrf2. MAPK and PI3K/Aktwere also analyzed due to their critical roles in the regulation of cellular redox homeostasis against oxidative damage. As a result, phosphorylation of extracellular regulated kinase (ERK) was significantly induced by lycopene treatment while the activated status of c-Jun NH2-terminal kinase (JNK), p38, and Akt, were not given any effect. To confirm the antioxidative mechanism of HO-1 mediated by ERK activation, each selective inhibitor was employed in a protection assay, in which oxidative damage occurred by t-BHP. Lycopene, SnPP, and CoPP treatments reflected accelerated HO-1 expression could be a protective role against oxidative damage-initiated cell death. A selective inhibitor for ERK significantly inhibited the lycopene-induced cytoprotective effect but selective inhibitors for other signaling molecules did not attenuate the rate of t-BHP-induced cell death. Conclusion: In conclusion, lycopene potently scavenged intracellular ROS formation and enhanced the HO-1 mediated antioxidative potential through the modulation of Nrf2, MAPK signaling pathway in RAW 264.7 cells.

• Journal of The Korean Society of Integrative Medicine
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• v.12 no.2
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• pp.121-131
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• 2024

Purpose : This study aimed to investigate the leisure satisfaction of participants in the Geod-Jyu walking program and to examine the relationship between health promotion behaviors and quality of life. In addition, we investigated the impact of leisure satisfaction with walking among program participants on health promotion behaviors and quality of life. By clarifying these relationships, we aimed to identify factors of leisure satisfaction with walking that could enhance participants' health promotion behaviors and quality of life. Methods : A survey was conducted among 301 participants enrolled in the Geod-Jyu walking program in Chungcheongnam-do, with 288 responses analyzed. Data analysis included Pearson's correlation, confirmatory factor analysis, and multiple regression analysis using SPSS 27.0. Result : Leisure satisfaction exhibited a positive correlation with health promotion behavior (r=.544, p<.01) and quality of life (r=.478, p<.01). Furthermore, health promotion behavior showed a positive correlation with quality of life (r=.636, p<.01). Leisure satisfaction positively influenced quality of life, with physiological satisfaction (β=.16, t=2.32) and relaxation satisfaction (β=.15, t=2.04) emerging as notable contributors. In addition, leisure satisfaction had a significant positive impact on health promotion behaviors, with psychological satisfaction (β=.24, t=3.09) and educational satisfaction (β=.20, t=3.09) playing key roles. Health promotion behavior had a positive impact on quality of life, with all sub variables exhibiting significant positive effects in the following order: self-actualization (β=.24, t=4.16), stress management (β=.22, t=3.97), exercise (β=.22, t=4.05), and health responsibility (β=.12, t=2.14). Conclusion : The findings indicate that factors related to physical and rest satisfaction, as well as health-promoting behaviors facilitated by walking, significantly affected the quality of life among Geod-Jyu participants. Thus, promoting leisure satisfaction and engaging in health-promoting activities through walking can enhance overall well-being. Encouraging participation in the Geod-Jyu walking program is critical for physical and psychological benefits, and for promoting healthier behaviors. Further development of Geod-Jyu is expected to enhance walking satisfaction, promote self-care skills for health management, and positively influence quality of life.

• 한국균학회소식:학술대회논문집
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• 2015.05a
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• pp.59-59
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• 2015

Cryptococcus neoformans causes life-threatening meningoencephalitis in humans, but the treatment of cryptococcosis remains challenging. To develop novel therapeutic targets and approaches, signaling cascades controlling pathogenicity of C. neoformans have been extensively studied but the underlying biological regulatory circuits remain elusive, particularly due to the presence of an evolutionarily divergent set of transcription factors (TFs) in this basidiomycetous fungus. In this study, we constructed a high-quality of 322 signature-tagged gene deletion strains for 155 putative TF genes, which were previously predicted using the DNA-binding domain TF database (http://www.transcriptionfactor.org/). We tested in vivo and in vitro phenotypic traits under 32 distinct growth conditions using 322 TF gene deletion strains. At least one phenotypic trait was exhibited by 145 out of 155 TF mutants (93%) and approximately 85% of the TFs (132/155) have been functionally characterized for the first time in this study. Through high-coverage phenome analysis, we discovered myriad novel TFs that play critical roles in growth, differentiation, virulence-factor (melanin, capsule, and urease) formation, stress responses, antifungal drug resistance, and virulence. Large-scale virulence and infectivity assays in insect (Galleria mellonella) and mouse host models identified 34 novel TFs that are critical for pathogenicity. The genotypic and phenotypic data for each TF are available in the C. neoformans TF phenome database (http://tf.cryptococcus.org). In conclusion, our phenome-based functional analysis of the C. neoformans TF mutant library provides key insights into transcriptional networks of basidiomycetous fungi and ubiquitous human fungal pathogens.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.34 no.2
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• pp.515-527
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• 2014

This study conducted experiment for understanding engineering characteristics of domestic sands by examining standard sand and sand from Yokji Island and Nakdong River in terms of confining pressure, $K_0$, over consolidation and relative density factors through triaxial compression test. The test showed that deviator stress by strain positively changed as confining pressure and relative density grow while $K_0$ and over consolidation factors do not directly correlated with it. Angle of internal friction decreases as confining pressure increases which strengthens contact force between particles, and declines as relative density drops, whereas $K_0$ and over consolidation factors hardly affect the results. When it comes to volumetric strain, volume expansion decreases as confining pressure increase due to crushability and rearrangement of particles while $K_0$ and over consolidation shows same movement unconditionally, and relative density appears compressed as it grows at the beginning however it expands as axial strain increases. Modulus of elasticity ($E_{sec}$) by strain has tendency into convergence resulting in initial secant modulus of elasticity ($E_{ini}$) > secant modulus of elasticity($E_{sec}$) > tangent modulus of elasticity ($E_{tan}$). On the other hand, it grows as confining pressure and relative density increase while indicating similar modulus of elasticity ($E_{sec}$) regarding on $K_0$ and over consolidation. Slope of critical line (M) tended to decrease as confining pressure increases, follow same line according to $K_0$, confining pressure and relative density, and increase as relative density grows.