Proceedings of the Korean Society of Embryo Transfer Conference
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2002.11a
/
pp.118-118
/
2002
Programmed cell death (PCD) is thought as a well-controlled process by which unwanted cells are selectively eliminated. During the last decade many researches have elucidated molecules and their interactions involved in cell death by using largely in vitro induction of cell death or survival signals in a more defined manner, While these critical information and novel findings provide us with clearer understanding of mechanisms underlying cell death, it does by no means explain how PCD occurs and which cells or tissues are affected during normal embryonic development in vivo. In this study, we used zebrafish to examine whether the PCD is occurring selectively or randomly in developing embryos by whole mount in situ TUNEL analysis with specific markers for neural cells. The result revealed that the degree and distribution of TUNEL staining varied considerably throughout gastrulation stage, and there was also a number of TUNEL-negative embryos. Most of TUNEL-positive cells were scattered randomly throughout the blastoderm. During the gastrulation stage about 75 % of the embryos analyzed exhibited more than 5 TUNEL-positive cells. As the dorsal epiblast begins to thicken rather abruptly near the end of gastrulation, TUNEL-positive cells were mainly located along the dorsal side. Although there were some variations in TUNEL staining during segmentation and pharyngeal stages, TUNEL staining continued to be localized to the central nervous system, and was also detected in the sensory organs, trigeminal ganglions, and the primary sensory neurons. High levels of the cell death in developing brain between 20-somite and prim-6 stages are thought to play a role in the morphogenesis and organization of the brain. At prim-16 stage, cell death is considerably reduced in the brain region. Dying cells are mainly localized to the prospective brain region where ectodermal cells are about to initiate neurogenesis. As development progressed, high levels and more reproducible patterns of cell death were observed in the developing nervous system. Intensive TUNEL staining was restricted to the trigeminal ganglions, the primary sensory neurons, and sensory organs, such as olfactory pits and otic vesicles. Thus, PCD patterning in zebrafish embryos occurs randomly at early stages and becomes restricted to certain region of the embryos. The spatio-temporal pattern of PCD during the early embryonic development in zebrafish will provide basic information for further studies to elucidate genes involved in. regulation of PCD largely unknown in vivo during vertebrate embryogenesis.
Park, Jong Youn;Park, Jae Min;Hong, Chang Gi;Kim, Kyong Min;Cho, Jae Kwon
Korean Journal of Ichthyology
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v.28
no.1
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pp.1-8
/
2016
This study was conducted to investigate the physiological responses of sevenband grouper, Epinephelus septemfasciatus at low temperature condition in winter season. Water temperature was dropped from $12^{\circ}C$ to $5^{\circ}C$ by two different interval (interval I: $-1^{\circ}C/24h$ and interval II: $-1^{\circ}C/8h$). The experimental fishes were exposed for 9 days at each water temperature condition, and were observed survival rate. The blood sample was collected from fishes for analysis of cortisol, glucose, total protein (TP), and electrolytes ($Na^+$, $Cl^-$, $K^+$). Mortality of fish was not observed until $5^{\circ}C$. The mortality (12 individuals) began after 170 h and all fish were death after 174 h in interval I case, whereas the mortality (9 individuals) began after 72 h and all fish were death after 76 h in interval II case. Little different was observed in the physiological responses, but trend was similar between two intervals. The physiological metabolism of plasma cortisol and glucose were increased rapidly, and swimming ability and respiration response was faster at $7^{\circ}C$. This result means that the critical temperature of sevenband grouper was $7^{\circ}C$ at low water temperature condition.
The Journal of the Institute of Internet, Broadcasting and Communication
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v.11
no.5
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pp.113-130
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2011
Due to the development of information and communication technology, the global influence on politics, economics, society, and culture has grown. A major example of this impact on the economic sector is the growth of e-commerce, which increases both the speed and efficiency of businesses. In light of these new developments, businesses need to shift away from the misconception that information overwhelms to embrace the enhanced competitiveness that e-commerce provides. However, concern about fraudulent transactions through e-commerce is pertinent because of the loss in both critical revenue and consumer confidence in open markets. Current solutions for fraudulent transactions include real-time monitoring and processing, payment pending, and confirmation through SMS, E-mail, and other wired means. Our research focuses on the management of Fraud Detection Systems (FDS) to safeguard online electronic payment systems. With effective implementation of our research we hope to foster an honorable online trading culture and protect consumers. Future comparative research in domestic and abroad markets would provide further insight into preventing fraudulent transactions.
An, Joonghyun;Youn, Jiae;Cho, Jeonghun;Park, Daejin
Journal of the Institute of Electronics and Information Engineers
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v.52
no.12
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pp.99-108
/
2015
The embedded microcontroller which is operated by the logic gates synchronized on the clock pulse, is gradually used as main controller of mission-critical systems. Severe electrical situations such as high voltage/frequency surge may cause malfunctioning of the clock source. The tolerant system operation is required against the various external electric noise and means the robust design technique is becoming more important issue in system clock failure problems. In this paper, we propose on-chip backup clock change architecture for the automatic clock failure detection. For the this, we adopt the edge detector, noise canceller logic and glitch-free clock changer circuit. The implemented edge detector unit detects the abnormal low-frequency of the clock source and the delay chain circuit of the clock pulse by the noise canceller can cancel out the glitch clock. The externally invalid clock source by detecting the emergency status will be switched to back-up clock source by glitch-free clock changer circuit. The proposed circuits are evaluated by Verilog simulation and the fabricated IC is validated by using test equipment electrical field radiation noise
Journal of the Institute of Electronics Engineers of Korea SP
/
v.44
no.1
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pp.17-25
/
2007
In most electronic imaging applications, image with high resolution(HR) are desired. HR means that pixel density within an image is high, and therefore HR image can offer more details that may be critical in various applications. Digital images that are captured by CCD and CMOS cameras usually have a very low resolution, which significantly limits the performance of image recognition systems. Image super-resolution techniques can be applied to overcome the limits of these imaging systems. Super-resolution techniques have been proposed to increase the resolution by combining information from multiple images. To techniques were consisted of the registration algorithm for estimation and shift, the nearest neighbor interpolation using weight of acquired frames and presented frames. In this paper, it is proposed the image interpolation techniques using the wavelet base function. This is applied to embody a correct edge image and natural image when expend part of the still image by applying the wavelet base function coefficient to the conventional Super-Resolution interpolation method. And the proposal algorithm in this paper is confirmed to improve the image applying the nearest neighbor interpolation algorithm, bilinear interpolation algorithm.,bicubic interpolation algorithm through the computer simulation.
Journal of the Korean Institute of Landscape Architecture
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v.38
no.4
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pp.45-53
/
2010
This paper has attempted to propose a more progressive direction for U-environmental sculptures in a ubiquitous urban space in terms of usability, service, function and formative characteristics that enhance user satisfaction with these sculptures. For this, Media Pole, a U-environmental sculpture on Gangnam U-Street, has been examined. The results of this study are as follows: First, a study of user satisfaction was planned after establishing levels of satisfaction in terms of service, function and formative characteristics. In terms of satisfaction by the section of Media Pole, the public transportation map was the most influential in service while street culture and beauty of shape were critical in function and formative characteristics, respectively. In the relationship between satisfaction by section and overall satisfaction, furthermore, functional satisfaction had the biggest influence. Therefore, the development of human-centered functional factors which have pleasant and touching stories to facilitate communication with citizens are important in order to develop various Media Pole contents to enhance user satisfaction with the U-environmental sculpture and allow it evolve into a Gangnam landmark Since we are in the initial stage of a U-city, there are many problems that need to be addressed such as a lack of natural beauty, poor material quality of environmental sculptures, absence of storytelling, poor technology, lack of content, poor profitability and lack of means of social communication. In the end, the development of a comfortable, people-friendly space and U-environmental sculptures are needed in consideration of economic, social and cultural aspects instead of focusing on advanced technology only.
The persent study aims at finding out a means of prevention cool spell damages on the hilly areas. The irrigation plots of 24 hour stored water warm water way and warm water plots, cool water way are respectively established to find out water temperature and influnce on the growing rice plants. The results obtained are summed up as follows. 1. Warm water areas consisted of $5 m^2 Q=0.93 1{\ell}/sec$, V=31 cm/sec, S=1/1, 000, L=81.6m, B=5cm, h=6cm, t=4min 33sec, drops=9 areas, are constructed to help the water temperature of $14.5^{\circ}C$ rise to that of $21.6^{\circ}C$. This indicates lower temperature than $23^{\circ}C$ of critical water temperature in irrigation facilities by $1.45^{\circ}C$ and than $26.2^{\circ}C$ of balanced water temperature of Seoul arears by $4.6^{\circ}C$. But this does not give much influance on rice plant cultivation. 2. The rising of water temperature is influened according to the temperature, solar radiation but the water temperature changes according to the heat absorption of organized materials, weather and terraces. The difference of water temperature could be found in the first growing stage. 3. Through the warm water way of water rises to the temperature of $21.6^{\circ}C$ which also rises to the temperature of around $30^{\circ}C$ in the paddy field of submerged irrigation. The rice plants are comparatively free from prolonged cool damage, reproduction abstructive damage. 4. The water temperature in rice field in proportion to temperature influence of weather condition but the water temperature approaches to that of weather in the days of later growing stage and water temperature become lower than the air temperature in the fruit stage. 5. The water in the submreged field is $10^{\circ}C$ warmer than in the warm water way during the first growing stage period but the water temperature in the warm water way is warmer in the later growing stage period. The cool water of $14.5^{\circ}C$ is warmed to $30.1^{\circ}C$ and rice plants cultivation is free from other damages. 6. The 12% increased production or 570.98kg/10a is made cool water plot by rising the temperature of water from $14.5^{\circ}C$ to $21.6^{\circ}C$ making the water run through warm water way. 7. The damage inflicted by the cool water irrigation during the first growing stage period is the obstruction of peak tillering stage and the obstruction of heading the later growing stage period and the obstruction of fruiting and number of panides per fill.
Korean Journal of Construction Engineering and Management
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v.11
no.3
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pp.125-133
/
2010
Although work-efficiency of construction machinery is a critical factor for estimating its workable-quantity perunit time, the efficiency figure table presented in the Poom-Sam that is used for Construction Cost Estimation of public sectors in Korea is very subjective for practical usage. In order to suggest objective work-efficiency table for a Pump-Dredger, domestic and overseas documentary records were investigated and on-going construction sites were also visited. Moreover, actual work quantities collected from the site visits were compared with the ones calculated based on the Standard Measurement Methods used in Japan. The research found that the table can be revised by means of detailing down by several factors, namely project type, depth of soil, undersea-site shape, and condition on the sea for better estimation of its workable-quantity. The research will be the foundation for applying the rapid development of Construction Equipment and technology to the appropriate cost estimations and the ground work of related studies.
The Journal of Korean Institute of Communications and Information Sciences
/
v.24
no.12A
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pp.2015-2024
/
1999
Presented in this paper are a new complex-umber filter architecture, which is suitable for an efficient implementation of baseband signal processing of digital communication systems, and a chip-set design of adaptive decision-feedback equalizer (ADFE) employing the proposed structure. The basic concept behind the approach proposed in this paper is to apply redundant binary (RB) arithmetic instead of conventional 2’s complement arithmetic in order to achieve an efficient realization of complex-number multiplication and accumulation. With the proposed way, an N-tap complex-number filter can be realized using 2N RB multipliers and 2N-2 RB adders, and each filter tap has its critical delay of $T_{m.RB}+T_{a.RB}$ (where $T_{m.RB}, T_{a.RB}$are delays of a RB multiplier and a RB adder, respectively), making the filter structure simple, as well as resulting in enhanced speed by means of reduced arithmetic operations. To demonstrate the proposed idea, a prototype ADFE chip-set, FFEM (Feed-Forward Equalizer Module) and DFEM (Decision-Feedback Equalizer Module) that can be cascaded to implement longer filter taps, has been designed. Each module is composed of two complex-number filter taps with their LMS coefficient update circuits, and contains about 26,000 gates. The chip-set was modeled and verified using COSSAP and VHDL, and synthesized using 0.8- μm SOG (Sea-Of-Gate) cell library.
In this paper we propose Fourier magnitudes based privacy preserving clustering on time-series data. The previous privacy-preserving method, called DFT coefficient method, has a critical problem in privacy-preservation itself since the original time-series data may be reconstructed from privacy-preserved data. In contrast, the proposed DFT magnitude method has an excellent characteristic that reconstructing the original data is almost impossible since it uses only DFT magnitudes except DFT phases. In this paper, we first explain why the reconstruction is easy in the DFT coefficient method, and why it is difficult in the DFT magnitude method. We then propose a notion of distance-order preservation which can be used both in estimating clustering accuracy and in selecting DFT magnitudes. Degree of distance-order preservation means how many time-series preserve their relative distance orders before and after privacy-preserving. Using this degree of distance-order preservation we present greedy strategies for selecting magnitudes in the DFT magnitude method. That is, those greedy strategies select DFT magnitudes to maximize the degree of distance-order preservation, and eventually we can achieve the relatively high clustering accuracy in the DFT magnitude method. Finally, we empirically show that the degree of distance-order preservation is an excellent measure that well reflects the clustering accuracy. In addition, experimental results show that our greedy strategies of the DFT magnitude method are comparable with the DFT coefficient method in the clustering accuracy. These results indicate that, compared with the DFT coefficient method, our DFT magnitude method provides the excellent degree of privacy-preservation as well as the comparable clustering accuracy.
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