• Title/Summary/Keyword: critical experiment

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Imaging of Fractures and Tunnel by 3-D ERT (전기비저항 토모그래피에 의한 파쇄대 및 터널의 3차원 영상화)

  • Yi, Myeong-Jong;Kim, Jung-Ho;Son, Jeong-Sul
    • Geophysics and Geophysical Exploration
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    • v.11 no.4
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    • pp.302-309
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    • 2008
  • ERT imaging, especially 3-D method, is a very powerful means to obtain a very high resolution image of the subsurface for geotechnical or hydrogeological problems. In this paper, we introduce two examples of successful case histories, where the imaging targets were three-dimensional. First example is the case of 3-D fracture imaging for hydrogeologic application. In this example, the borehole deviation was a critical problem in the ERT imaging and we could obtain real 3-D attitude of fracture system by including the borehole deviation in the inversion. In the second case, we did field experiment to image the empty tunnel with the size of $2m{\times}2m$ and the target was very clearly imaged in 3-D space. In these examples, we could show that 3-D ERT imaging is a very powerful tool for the 3-D subsurface imaging and the method can provide enhanced imaging capabilities especially for the 3-D targets such as fractures and cavities or tunnel.

Cryopreservation with Trehalose Reduced Sperm Chromatin Damage in Miniature Pig

  • Park, Cheol-Ho;Kim, Sung-Won;Hwang, You-Jin;Kim, Dae-Young
    • Journal of Embryo Transfer
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    • v.27 no.2
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    • pp.107-111
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    • 2012
  • Miniature pig sperm cryopreservation is continually researched in biotechnology for breed conservation and reproduction. It is important to control the temperature at each stage of cryopreservation and cryoprotectant. It is also necessary to find the optimal cryoprotectant concentration and chemical elements of the extender. Recently, many studies have used various cryoprotectant materials, such as dimethyl sulphoxide (DMSO), ethylene glycol (EG), antifreeze protein (AFP), amides, and glycerol. Glycerol is a commonly used cryoprotectant. However, glycerol has critical cytotoxic properties, including osmotic pressure and it can cause irreversible damage to live cells. Therefore, We focused on membrane fluidity modifications can reduce cell damage from freezing and thawing procedures and evaluated on the positive effects of trehalose to the viability, chromatin integrity, and motility of boar sperm. Miniature pig sperm was separated from semen by washing with modified- Modena B (mMB) extender. After centrifugation, the pellet was diluted with the prepared first extender. This experiment was designed to compare the effects that sperm cryopreservation using two different extenders has on sperm chromatin. The control group used the glycerol only and it was compared with the glycerol and glycerol plus trehalose extender. Sperm viability and motility were evaluated using WST1 assays and computer-assisted semen assays (CASA). Chromatin structure was examined using acridine orange staining. For the motility descriptors, trehalose caused a significant (p<0.01) increase in total motility ($57.80{\pm}4.60%$ in glycerol vs. $75.50{\pm}6.14%$ in glycerol + trehalose) and progressive ($51.20{\pm}5.45%$ in glycerol vs. $70.74{\pm}8.06%$ in glycerol + trehalose). A significant (p<0.05) increase in VAP ($42.70{\pm}5.73{\mu}m/s$ vs. $59.65{\pm}9.47{\mu}m/s$), VSL ($23.06{\pm}3.27{\mu}m/s$ vs. $34.60{\pm}6.58{\mu}m/s$), VCL ($75.36{\pm}11.36{\mu}m/s$ vs. $99.55{\pm}12.91{\mu}m/s$), STR ($54.4{\pm}2.19%$ vs. $58.0{\pm}1.63%$), and LIN ($32.2{\pm}2.05%$ vs. $36.0{\pm}2.45%$) were also detected, respectively. The sperm DNA fragmentation index was 48.8% to glycerol only and 30.6% to glycerol plus trehalose. Trehalose added group showed higher percentages of sperm motility, stability of chromatin structure than glycerol only. In this study, we suggest that trehalose is effective in reducing freezing damage to miniature pig sperm and can reduce chromatin damage during cryopreservation.

The Influences of Rice Straw and Gypsum Applied to a Saline Soil on the Growth Status of rice Seedlings when Flooded Direct Sowing (볏짚 및 석고시용(石膏施用)이 간척답(干拓畓) 직파(直播)벼의 초기생육(初期生育) 장해(障害)에 미치는 영향(影響))

  • Hwang, Seon-Woong;Lee, Choon-Soo;Lee, Yong-Jae;Kwak, Han-Kang;Park, Nae-Jung
    • Korean Journal of Soil Science and Fertilizer
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    • v.23 no.1
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    • pp.34-39
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    • 1990
  • The experiment was conducted in the pots of flooded saline paddy soil to evaluate influence of rice straw and gypsum application on germination and early growth status of directly sowed rice seedlings. 1. Germination percentage of rice seedlings were higher in treatment sowing at 1 day after submergence than that of treatment sowing at 21 days after submergence, and was severely interrupted by rice straw application. 2. Application of rice straw promoted the release of bicarbonate and volatile fatty acid of submerged water, while the amount of sulfate and soluble cations were decreased as compared to control. 3. Germination percentage of rice seedling had significant negative correlations with chemical characters of submerged water, and was highly affected by submerged water at 7 days after sowing. 4. Rice straw application interrupted germination of rice seedling by increasing pH of submerged water over critical level, and gypsum application depressed the early growth of young seedling dues to high salt concentration. 5. The relaease of bicarbonate was remarkedly increased with increasing pH over 7.5 of submerged water.

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Characteristics of Size-segregated Mass Concentrations of Indoor Aerosol Particles in University Buildings (대학건물 실내 에어로졸입자의 입경별 질량농도 특성)

  • Suh, Jeong-Min;Wang, Bin;Jang, Seong-Ho;Park, Jeong-Ho;Choi, Kum-Chan
    • Journal of Korean Society of Occupational and Environmental Hygiene
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    • v.24 no.4
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    • pp.453-461
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    • 2014
  • Objective: Based on the fact that fine particles are more likely to produce negative influences on the health of occupants as well as the quality of indoor air compared to coarse particles, it is critical to determine concentrations of aerosol particles with different sizes. Thus, this study focused on the size distribution and concentrations of aerosol particles in university buildings. Method: Aerosol particles in indoor air were collected from four areas: corridors in buildings(In-CO), lecture rooms(In-RO), laboratories(In-LR), and a cafeteria(In-RE). Samples were also collected from outside for comparison between the concentrations of indoor and outdoor particles. For the collection of the samples, an eight stage non-viable cascade impactor was used. Result: The average concentration of $PM_{10}$ in the samples collected from indoor areas was $34.65-91.08{\mu}g/m^3$,and the average for $PM_{2.5}$ was $22.65-60.40{\mu}g/m^3$. The concentrations of the aerosol particles in the corridors, lecture rooms, and laboratories were relatively higher than the concentrations collected from other areas. Furthermore, in terms of mass median aerodynamic diameter(MMAD), the corridors and lecture rooms had higher numbers due to their characteristics, showing $2.36{\mu}m$ and $2.11{\mu}m$, respectively. Laboratories running an electrolysis experiment showed $1.58{\mu}m$, and the cafeteria with regular maintenance and ventilation had $1.96{\mu}m$. Conclusion: The results showed that the $PM_{10}$ concentrations of all samples did not exceed indoor air quality standards. However, the $PM_{2.5}$ concentration was over the standard and, in particular, the concentration of fine particles collected from the laboratories was relatively higher, which could be an issue for the occupants. Therefore, it is important to improve the quality of the indoor air in university buildings.

Comparison of Aviary, Barn and Conventional Cage Raising of Chickens on Laying Performance and Egg Quality

  • Ahammed, M.;Chae, B.J.;Lohakare, J.;Keohavong, B.;Lee, M.H.;Lee, S.J.;Kim, D.M.;Lee, J.Y.;Ohh, S.J.
    • Asian-Australasian Journal of Animal Sciences
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    • v.27 no.8
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    • pp.1196-1203
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    • 2014
  • This study intended to compare the productive performance of three different layer raising systems; conventional cage (CC), barn (BR) and aviary (AV). The AV is welfare bestowed housing that allows free locomotion for birds within the BR. The BR allows bird's free locomotion inside BR but without multilevel structures. Both pullets and cockerels were housed together in both AV and BR, but only pullets in CC. Seventeen weeks old Lohmann Brown Lite (n = 800) pullets were housed in AV during this study. The same age layer pullets were simultaneously assigned to either at CC or BR to compare egg production performance with AV. The duration of experiment was 40 weeks (from 21st to 60th week). There were no remarkable differences in egg production, hen day egg production (HDEP) and average egg weight among three rearing systems. First 20 weeks (phase-1) average HDEP (%) of AV, CC, and BR were 85.9, 88.8, 87.1 and average egg weights (g) were 57.5, 59.9, and 56.9 respectively. Those of the remaining 20 weeks (phase-2) were 87.1, 87.9, 85.5 and 64.2, 63.0 62.1, respectively. Daily feed intakes (122 g, 110 g, 125 g); feed conversion ratio (2.4, 2.1, 2.5) and daily egg mass (53.9 g, 54.4 g, 52.8 g) data from AV, CC, and BR were not influenced significantly by the respective raising systems. Daily feed intake of layers in both AV (124 g) and BR (127 g) tended to be higher than that in CC (113 g) during phase-2. Overall, exterior egg quality (dirty and cracked eggs) in both phases was superior in BR compared with AV and CC, whereas CC generated intermediate results. This study indicated that the HDEP per se in AV and BR were not significantly different from that in CC. The study implied that the facility depreciation cost for AV and cost for increased feed intake in AV compared to CC are believed to be critical to evaluate the cost effectiveness of egg production in AV.

Warpage of Flexible OLED under High Temperature Reliability Test (고온 신뢰성 시험에서 발생된 플렉서블 OLED의 휨 변형)

  • Lee, Mi-Kyoung;Suh, Il-Woong;Jung, Hoon-Sun;Lee, Jung-Hoon;Choa, Sung-Hoon
    • Journal of the Microelectronics and Packaging Society
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    • v.23 no.1
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    • pp.17-22
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    • 2016
  • Flexible organic light-emitting diode (OLED) devices consist of multi-stacked thin films or layers comprising organic and inorganic materials. Due to thermal coefficient mismatch of the multi-layer films, warpage of the flexible OLED is generated during high temperature process of each layer. This warpage will create the critical issues for next production process, consequently lowering the production yield and reliability of the flexible OLED. In this study, we investigate the warpage behavior of the flexible OLED for each bonding process step of the multi-layer films using the experimental and numerical analysis. It is found that the polarizer film and barrier film show significant impact on warpage of flexible OLED, while the impact of the OCA film on warpage is negligible. The material that has the most dominant impact on the warpage is a plastic cover. In order to minimize the warpage of the flexible OLED, we estimate the optimal material properties of the plastic cover using design of experiment. It is found that the warpage of the flexible OLED is reduced to less than 1 mm using a cover plastic of optimized properties which are the elastic modulus of 4.2 GPa and thermal expansion coefficient of $20ppm/^{\circ}C$.

A Study on Penetration of Pea Seedling Taproots as Influenced by strength of Soil (토양(土壤)의 경도(硬度)가 완두뿌리의 신장(伸長)에 미치는 영향(影響))

  • Jo, In-Sang;Cho, Seong-Jin;Im, Jeong-Nam
    • Korean Journal of Soil Science and Fertilizer
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    • v.10 no.1
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    • pp.7-12
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    • 1977
  • This experiment was conducted in the laboratory in order to find out the relationships between the root growth and soil physical properties. The soils selected for this study were Sangju sandy loam, Yeongog loam, Hwadong silty clay loam, which have been considered to be a typical upland soils of Korea. Artificial core samples were made with various moisture contents and bulk densities. Elongation rate of pea seedling taproot and soil strength were measured respectively in these core samples. The results obtained are summarized as follows: 1. The soil strength increased with the bulk density and deceased with moisture content. 2. The correlation between root elongation and soil bulk density was significantly recognized at the same moisture content and the root elongation was influenced by the bulk density more significantly at dry condition. 3. The elongation rate of pea seedling taproot was significantly decreased by increasing the strength (Yamanaka tester and Fine probe) of the soils. 4. The soil strength of $21kg/cm^2$ in fine metal probe or 24mm in Yamanaka tester was considered to be the critical point for plant growth, which was restricting root elongation smaller than 1/4 of the maximum growth rate.

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Varietal Differences and Time Course Changes in Greenness Values in Rice Leaf (벼 엽록도값의 품종간 차이와 생육기간중 경시적 변화)

  • 홍광표;김영광;정완규;손길만;송근우;최용조;최진용
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.48 no.6
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    • pp.479-483
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    • 2003
  • Knowledge of N accumulation during the vegetative growth stage of rice (Oryza sativa L.) is useful for determining the need for topdressing fertilizer N at panicle initiation and booting stage. The chlorophyll content showing SPAD threshold values generated by a simple and portable diagnostic instrument, chlorophyll meter, enables implement the measures of greenness and/or relative chlorophyll contents of leaves. Two-year field experiment was conducted to evaluate the possible application of the chlorophyll meter for N diagnosis in transplanted paddy rice. The chlorophyll meter threshold values (SPAD reading) were taken from uppermost fully expanded leaves at 10days interval from 10 days after transplanting. SPAD readings and N contents of rice leaf blade were closely related at the maximum tillering stage. SPAD readings during growth period showed M-shaped distribution, sharply finished to drop at 20 days after heading date. As N fertilizer increased, the SPAD readings increased. The SPAD readings showed more critical and clear difference in immature paddy field than in ordinary paddy field, where the former soil showed higher response to N fertilizer topdressed. Based on the PAD readings for obtaining an optimum sink size bearing the maximum grain yield, N fertilizer to be dressed at the panicle initiation stage is to be adjusted within the SPA$\times$tillers value range 888 in ordinary paddy, and 800 in immature paddy vice. Among the cultivars tested, Huckhyangbyeo, Jinpumbyeo, Ansungbyeo, Sobibyeo, Manpungbyeo, Sangmibyeo, Jinbongbyeo, showed high SPAD values, whereas the cultivars, Nonghobyeo, Saechuchungbyeo, Hwabongbyeo, Mananbyeo, did low values and others intermediate SPAD threshhold values.

Study on Relationship between Elderly Group Lifestyle and Selection Attributes in the Health Functional Foods (실버층 라이프스타일에 따른 건강기능식품 선택속성에 관한 연구)

  • Lee, Myung Sook;Kim, Sook Eung
    • Korean Journal of Clinical Pharmacy
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    • v.25 no.4
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    • pp.286-295
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    • 2015
  • Objective: This experiment is to study how elderly group and their various lifestyles interact with health functional foods, according to their selection behavior. Different lifestyles will be observed closely, as well as how different health conditions and consumer involvements will affect critical decision making in selecting health functional foods. Method: Theories and discoveries from original advanced research were compared parallel to the new study. Results: First, cluster analysis and exploratory analysis were performed amongst different elder lifestyles. Lifestyle exploratory analysis was used for healthy, unique, leisure, and economical-style elders. Cluster analysis was used for material trend oriented, health oriented, complacent oriented-style elders. Health Functional Foods' selection trait Exploratory Factor Analysis showed that product's originality (function, uniqueness, specialty, compatibility, distributor, expiration date), quality (amount, daily dose, visual representation, accessibility, portability, natural ingredients), and popularity (product container, brand image, taste and smell, advertised product, domestic or import, well-known function) were the three main causes. Secondly, the amount of benefits for the elderly group health lifestyle were affected by 'Interest in health', 'Notability of the health functional food', and 'Functionality approved mark'. Specifically, the importance of, 'Interest in health', 'Notability of health functional food', and 'Functionality approved mark' were noticeably high within health oriented elders. Lastly, after examining the data from elder lifestyle's relationship with health functional food selection trait, all three different results showed equal importance. If you closely examine material trend oriented elderly group, selection trait showed distinctively high regards in 'Fundamental Attribute', 'Typical Attribute', and 'Cognitive Attribute'. Health oriented elders showed their distinctively high regards in 'Natural Attribute', and less consideration in 'Typical Attribute' and 'Cognitive Attribute'. Complacent oriented-style elderly group showed less focus on 'Fundamental Attribute', and even less in 'Typical Attribute', and 'Cognitive Attribute'. Health oriented elderly group concluded with above data from the fact that they showed most importance and involvement in health beneficial products that are scientifically proven. Material trend oriented elderly group showed balanced traits in their concluded data, showing that they prefer function, safety, as well as the brand image and their reputation. Also, they consider the products' outer elements, such as design and product name, in order to sense inner functions. Conclusion: So, Silver Business corporations must develop products to fulfill the market demands, and strategize marketing plans to better target the correct audience.

Feasibility Analysis of Wasted Limestone Powder as a Viscosity Reducing Material for Cement Based Materials (시멘트 계열 재료의 점도 저하용 혼화재료로서 폐석회석 미분말의 사용 가능성 분석)

  • Lee, Hyang-Seon;Jeon, Jong-Un;Son, Bae-Geun;Han, Dongyeop
    • Journal of the Korean Recycled Construction Resources Institute
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    • v.6 no.2
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    • pp.123-129
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    • 2018
  • The aim of the research is providing a fundamental idea of reducing viscosity of cement based materials by replacing powder based material. With developing concrete technology, high performance concrete with high solid volume fraction has been used widely. Under the conditions of the high solid volume fraction due to the low w/c and replacement of SCMs, decreased fluidity is one of the critical problem, and thus plasticizer has been used to improve fluidity of the mixture. However, in rheological aspect, the fluidity of cement based materials can be defined with yield stress and viscosity, and using plasticizer only decreases yield stress without least controlling on viscosity. Therefore, based on the idea of Krieger-Dougherty model, a feasibility of wasted limestone powder from cement manufacturing process was used to decrease the viscosity of the mixture by replacing cement powder. According to a series of experiment, by replacing wasted limestone powder solely, there was a possibility of reducing viscosity was observed. Thus, in this research scope, it is considered to contribute on providing a fundamental idea of reducing viscosity with powder replacement and it is expected to contribute on further research using various conditions of replacing powders for reducing viscosity of cementitious materials.