• Journal of Microbiology and Biotechnology
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• v.31 no.1
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• pp.25-32
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• 2021

Inflammatory reactions activated by lipopolysaccharide (LPS) of gram-negative bacteria can lead to severe septic shock. With the recent emergence of multidrug-resistant gram-negative bacteria and a lack of efficient ways to treat resulting infections, there is a need to develop novel anti-endotoxin agents. Antimicrobial peptides have been noticed as potential therapeutic molecules for bacterial infection and as candidates for new antibiotic drugs. We previously designed the 9-meric antimicrobial peptide Pro9-3 and it showed high antimicrobial activity against gram-negative bacteria. Here, to further examine its potency as an anti-endotoxin agent, we examined the anti-endotoxin activities of Pro9-3 and elucidated its mechanism of action. We performed a dye-leakage experiment and BODIPY-TR cadaverine and limulus amebocyte lysate assays for Pro9-3 as well as its lysine-substituted analogue and their enantiomers. The results confirmed that Pro9-3 targets the bacterial membrane and the arginine residues play key roles in its antimicrobial activity. Pro9-3 showed excellent LPS-neutralizing activity and LPS-binding properties, which were superior to those of other peptides. Saturation transfer difference-nuclear magnetic resonance experiments to explore the interaction between LPS and Pro9-3 revealed that Trp3 and Tlr7 in Pro9-3 are critical for attracting Pro9-3 to the LPS in the gram-negative bacterial membrane. Moreover, the anti-septic effect of Pro9-3 in vivo was investigated using an LPS-induced endotoxemia mouse model, demonstrating its dual activities: antibacterial activity against gram-negative bacteria and immunosuppressive effect preventing LPS-induced endotoxemia. Collectively, these results confirmed the therapeutic potential of Pro9-3 against infection of gram-negative bacteria.

• KIPS Transactions on Software and Data Engineering
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• v.11 no.4
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• pp.179-188
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• 2022

There have been many successful researches on a bearing fault diagnosis based on Deep Learning, but there is still a critical issue of the data distribution difference between training data and test data from their different working conditions causing performance degradation in applying those methods to the machines in the field. As a solution, a data adaptation method has been proposed and showed a good result, but each and every approach is strictly limited to a specific applying scenario or presupposition, which makes it still difficult to be used as a real-world application. Therefore, in this study, we have proposed a method that, using a data transformation with MFCCs and a simple CNN architecture, can perform a robust diagnosis on a target domain data without an additional learning or tuning on the model generated from a source domain data and conducted an experiment and analysis on the proposed method with the CWRU bearing dataset, which is one of the representative datasests for bearing fault diagnosis. The experimental results showed that our method achieved an equal performance to those of transfer learning based methods and a better performance by at least 15% compared to that of an input transformation based baseline method.

• Geomechanics and Engineering
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• v.28 no.4
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• pp.335-346
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• 2022

Buckling failure is a typical slope instability mode that should be paid more attention to. It is difficult to provide systematic guidance for the monitoring and management of such slopes due to unclear mechanism. Here we examine buckling failure as the potential instability mode for a slope above a railway tunnel in southwest China. A comprehensive model test system was developed that can be used to conduct buckling failure experiments. The displacement, stress, and strain of the slope were monitored to document the evolution of buckling failure during the experiment. Monitoring data reveal the deformation and stress characteristics of the slope with different slipping mass thicknesses and under different top loads. The test results show that the slipping mass is the main subject of the top load and is the key object of monitoring. Displacement and stress precede buckling failure, so maybe useful predictors of impending failure. However, the response of the stress variation is earlier than displacement variation during the failure process. It is also necessary to monitor the bedrock near the slip face because its stress evolution plays an important role in the early prediction of instability. The position near the slope foot is most prone to buckling failure, so it should be closely monitored.

• The Journal of the Acoustical Society of Korea
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• v.42 no.5
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• pp.395-401
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• 2023

Sound Event Detection (SED) aims to identify not only sound category but also time interval for target sounds in an audio waveform. It is a critical technique in field of acoustic surveillance system and monitoring system. Recently, various models have introduced through Detection and Classification of Acoustic Scenes and Events (DCASE) Task 4. This paper explored how to design optimal parameters of DenseNet based model, which has led to outstanding performance in other recognition system. In experiment, DenseRNN as an SED model consists of DensNet-BC and bi-directional Gated Recurrent Units (GRU). This model is trained with Mean teacher model. With an event-based f-score, evaluation is performed depending on parameters, related to model architecture as well as model training, under the assessment protocol of DCASE task4. Experimental result shows that the performance goes up and has been saturated to near the best. Also, DenseRNN would be trained more effectively without dropout technique.

• International conference on construction engineering and project management
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• 2022.06a
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• pp.269-276
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• 2022

The robustness of a supply chain (i.e., the ability to cope with external and internal disruptions and disturbances) becomes more critical in ensuring the success of a construction project because the supply chain of today's construction project includes more and diverse suppliers. Previous studies indicate that topological features of the supply chain critically affect its robustness, but there is still a great challenge in characterizing and quantifying the impact of network topological features on its robustness. In this context, this study aims to identify network measures that characterize topological features of the supply chain and evaluate their impact on the robustness of the supply chain. Network centrality measures that are commonly used in assessing topological features in social network analysis are identified. Their validity in capturing the impact on the robustness of the supply chain was evaluated through an experiment using randomly generated networks and their simulations. Among those network centrality measures, the PageRank centrality and its standard deviation are found to have the strongest association with the robustness of the network, with a positive correlation coefficient of 0.6 at the node level and 0.74 at the network level. The findings in this study allows for the evaluation of the supply chain network's robustness based only on its topological design, thereby enabling practitioners to better design a robust supply chain and easily identify vulnerable links in their supply chains.

• Progress in Superconductivity and Cryogenics
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• v.9 no.2
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• pp.23-26
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• 2007

The influence of water partial pressure in Metal-organic Deposition (MOD) method was investigated on the texture and the morphology of $YBa_2Cu_3O_{7-x}$ (YBCO) films grown on the buffered metal tapes. The water partial pressure was varied from 4.2% up to 10.0% with the other process variables, such as annealing temperature and oxygen partial pressure, kept constant. In this work, the fluorine-free Y & Cu precursor solution added with Sm was synthesized and coated by the continuous slot-die coating & calcination step. The next annealing step of the YBCO films was done by the reel-to-reel method with the gas flowed vertically down. From the x-ray diffraction analysis, the un-reacted phase like $BaF_2$ peak was found at the water partial pressure of 4.2%, but $BaF_2$ peak intensity is much reduced as the water partial pressure is increased. However, the higher water partial pressure of about 10% in this experiment leads to the poor crystallinity of YBCO films. The morphologies of the YBCO films were not different from each other when the water partial pressure was varied in this work. The maximum critical current density of 3.8MA/$cm^2$ was obtained at the water partial pressure of 6.2% with the annealing temperature of 780$^{\circ}C$ and oxygen partial pressure of 500ppm.

• Journal of Veterinary Clinics
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• v.40 no.1
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• pp.1-7
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• 2023

The growing market for companion animals, combined with their increasing lifespan, has generated an increased interest in companion animal immunity enhancers. Ginsenoside, a saponin component of ginseng and an essential ingredient of red ginseng marc (produced during red ginseng production), is effective in improving immunity. In this experiment, a powder mixture of red ginseng marc and steamed red ginseng extract powder (RGME) was orally administered to dogs for eight weeks. Subsequently, blood samples were collected and tested every four weeks. In addition, canine peripheral blood mononuclear cells (cPBMCs) were stimulated with or without interleukin-2 (IL-2) to evaluate their proliferation and cytokine secretion abilities. Proliferation assay suggests that the administration of RGME effectively enhanced numbers of cPBMCs under IL-2 stimulation. Furthermore, in the RGME group, a significant increase in the concentration of interferon gamma released from cPBMCs under IL-2 stimulation was observed. In conclusion, RGME might be an effective health supplement for improving immunity in dogs.

• Journal of the Korea Society of Computer and Information
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• v.14 no.10
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• pp.189-197
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• 2009

As the web service technology matures. research is focused on the composite web services that combine individual web services within an enterprise or between enterprises. Quality of service is the critical competitiveness factor in this mature technology stage where there are many services with similar functionalities differing only in some non-functional properties. Monitoring is the key component for the service quality management of a web service. A service quality monitoring system design using a broker is presented in this paper. OWL-S is used to specify the composite service process and a service policy (inputs and outputs of each service, quality attributes and values, etc.) built by WS-Policy is applied to the composite service process. If there is any discrepancy between the service policy and the monitored data, the service provider and the user are notified of it so as to take necessary measures. We have implemented a travel reservation system as an example of the presented design and the experimental results are shown. Average response time was monitored and the timeout policy was applied in the experiment.

• Journal of Korea Society of Industrial Information Systems
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• v.28 no.6
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• pp.99-105
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• 2023

Submarines, which require a high degree of survivability, are among the most critical combat weapon systems in military strategic assets. Conventional submarines need air to operate their propulsion systems. Exhaust gases released into the water during snorkel navigation heat the surrounding fluid, producing a temperature wake. This wake, in turn, reduces the submarine's survivability. In this study, we conducted a preliminary experiment on the temperature traces formed by an underwater submarine's waste discharge. For this purpose, we collected propulsion system and navigation condition data from domestically introduced submarines and developed an experimental system to measure the temperature traces. As a result, we observed that high-temperature bubbles injected into the tank broke down into smaller sizes, and their temperature dropped to levels similar to the surrounding fluid. This observation was confirmed using a thermocouple sensor. Consequently, the thermal imaging system designed to measure the temperature trace of the water's surface did not detect any significant temperature traces.

• Journal of Periodontal and Implant Science
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• v.23 no.1
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• pp.77-96
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• 1993

This in vitro study was undertaken to observe whether citric acid application aids the attachment and proliferation of human periodontal ligament cells to the root surfaces of periodontally diseased teeth. The roots were prepared so that the comparison could be made among the control healthy root surface, citric acid demineralized and non-demineralized root planted surfaces. Prior to the cell attachment experiment, each groups were prepared for scanning electron microscopic (SEM) examinations of root surface morphology, All specimens were fixed with phosphate buffered glutaraldehydes, postfixed with phosphate buffered osmium tetraoxide and stained with phosphate buffered tannic acid. dehydrated in ethanol, critical point dried, sputter coated with gold and examined under the SEM. In the cell attachement experiment, human cultured periodontal ligament cells at concentration to $4.5{\times}\;10^4\;cells/ml$ were seeded in each culture well which contained prepared roots and incubated for 30min 1, 2, 6, 12 and 24 hours at 37, 5% $CO_2$air incubator. Than the specimens were prepared for SEM examination using, the same methods as described above. In the cell proliferation experiment, $5{\times}\;10^4\;cells/ml$ cells were seeded incubated with the specimens for 6 hours. Then, all of the specimens were moved into fresh culture well and incubated for 24, 48, and 72 hours. The cell counting was done after trypsinization, under light microscope. The results were as follows. When viewed the surface morphology prior to the cell attachment, the non acid treated root planed surface displayed scaling striation and occasional bacteria and calculus. The citric acid treated specimens displayed little debris on the surface and funnel shaped orifices of dentinal tubules. There were no apparent differences in the morphology of cells attached to the control and experiment groups. However, in initial attachement, there was a slight more enhanced appearance in attachment in citric acid treated groups than other root surfaces. After 6 hours of incubation, most of the cells initiated the alteration of cell morphology from ovoid to spindle shapes. After 24 hours of incubation, most of the cells displayed proliferated appearance and connected with each other via numerous processes. In the cell proliferation experiments, there were statistically significant increased number of cells in citic acid treated groups than other groups.