• 대한수의학회지
• /
• 제39권5호
• /
• pp.951-960
• /
• 1999

We performed this study to evaluate the potential clinical marker of urinary trypsinogen-2 together with amylase, lipase and urinary amylase creatinine clearance ratio (ACCR) for the diagnosis of acute pancreatitis in dogs. In the experiment on daily changing patterns of amylase, lipase and ACCR measurements in experimentally induced pancreatitis dogs, compared to values measured in pre-induction state, significant difference was seen in amylase until 5th day of induction, and for lipase significant difference was found during the 7th day of observation period (p < 0.05). No significant difference was found in ACCR for the study period (p > 0.05). On SDS-PAGE analysis of urine from experimentally induced pancreatitis dog, The 26kd band was markedly increased compared with that of normal state and that band was confirmed trypsinogen-2 using substrate interaction and isoelectric focusing assay after being eluted. When assessing the appearance of 26kd band on urine SDS-PAGE 87.1% (range: 50~100%) of experimentally induced pancreatitis dogs showed positive results, whereas no corresponding band was seen in dog without pancreatic disorders. With this result, determination of urinary trypsinogen-2 assay was found to have a high diagnostic value with a 70% of sensitivity and 100% of specificity as a routine test for pancreatitis, although the detection of trypsinogen-2 in urine can be varied on the progression stage of pancreatitis at the initial visit to animal clinic. We therefore suggest that the promising results in this study be used for the development of dipstick test for detecting acute pancreatitis in the future research.

• Asian Pacific Journal of Cancer Prevention
• /
• 제16권3호
• /
• pp.1117-1122
• /
• 2015

Background: Cisplatin is still used as a first-line medication for solid tumors. Nephrotoxicity is a serious side effect that can decrease renal function and restrict applicable doses. This research aimed to obtain the profile of cisplatin-induced nephrotoxicity and its associated factors in adult cancer patients at Dharmais National Cancer Hospital (DNCH). Materials and Methods: The design was cross-sectional with data obtained from patient medical records. We retrospectively reviewed adult cancer patients treated with cisplatin ${\geq}60mg/m^2$ for at least four consecutive chemotherapy cycles from August 2011 to November 2013. The nephrotoxicity criterion was renal function decline characterized by creatinine clearance <60 ml/min using the Cockroft-Gault (CG) equation. Results: Eighty-eight subjects received at least four chemotherapy cycles of cisplatin. The prevalence of cisplatin nephrotoxicity was 34.1%. Symptoms could be observed after the first cycle of chemotherapy, and the degree of renal impairment was higher with increased numbers of cycles (r=-0.946, $r^2=89.5%$). Factors that affected the decline of renal function were patient age (p=0.008, OR=3.433, 95%CI= 1.363-8.645) and hypertension (p=0.026, OR=2.931, 95%CI=1.120-7.670). Conclusions: Cisplatin nephrotoxicity occurred in more than one-third of patients after the fourth cycle of chemotherapy and worsened after each cycle despite preventive strategies such as hydration. The decline of renal function induced by cisplatin ${\geq}60mg/m^2$ was affected by age and hypertension.

• 대한한의학회지
• /
• 제18권2호
• /
• pp.214-222
• /
• 1997

The aim of this experiments was to investigate the effect of Croton. Tiglii. semen water extract on the renal function, plasma renin activity, plasma levels of atrial natriuretiu peptide and aldosterone in rats. The results of this study were as follows: 1. Water balance was not changed significantly after the administration of Croton Tiglii semen water extract. 2. Urine volume decreased significantly after the administration of Croton Tiglii semen water extract $80{\mu}l/200g$. 3. Urinary excretion of sodium increased significantly after the administration of Croton Tiglii semen water extract $40{\mu}l/200g$, but decreased significantly after the administration of Croton Tiglii semen water extract $80{\mu}l/200g$. 4. Urinary excretion of potassium decreased significantly after the administration of Croton Tiglii semen water extract $80{\mu}l/200g$. 5. Urinary excretion of chloride was not changed significantly after the administration of Croton Tiglii semen water extract. 6. Free water clearance was not changed significantly after the administration of Croton Tiglii semen water extract. 7. Urinary excretion of creatinine increased significantly after the administration of Croton Tiglii semen $40{\mu}l/200g$. 8. Plasma renin activity was not changed significantly after administration of Croton Tiglii semen water extract. 9. Plasma levels of atrial natriuretic peptide increased significantly after administration of Croton Tiglii semen water extract. 10. Plasma levels of aldosterone increased significantly after administration of Croton Tiglii semen $40{\mu}l/200g$.

• 대한한의학방제학회지
• /
• 제17권2호
• /
• pp.187-194
• /
• 2009

The kidney toxicities of WK-38 used for improvement of the vascular diseases, was examined using male and female Sprague-Dawley rats. The male and female rats were divided into 4 groups for intragastrical treatment with doses of 0, 5, 50, and 500 mg/kg/day for 13 weeks, respectively. In all male and female rats treated with WK-38, no mortality and gross pathological findings were shown for 13 weeks. There was substantially no change in body weight in all rats with treatment of WK-38. Urine osmolality as renal functional parameters were not exchanged in all rats treated with WK-38. The renal functional parameters including electrolytes excretory rate, creatinine clearance, and solute-free water reabsorption were significantly augmented on account of increase in urinary volume in female rats treated with WK-38, but not male. In summary, this study demonstrates that WK-38 exhibits no toxicity on kidney in all male and female rats.

• 대한약리학회지
• /
• 제5권2호
• /
• pp.141-148
• /
• 1969

The direct effect of isoproterenol on renal function, when given intravenously, is usually obscured by its potent hypotensive action. To obviate the latter action, isoproterenol was infused directly into one renal artery of the dog, the other kidney serving as a control for the general action. And following results were obtained. In the first series of experiments, the directic action of isoproterenol was ascertained. $1.0\;{\mu}g/kg/min$. reduced on both kidneys the urine flow, clearances of PAH and creatinine, as well as the amount of sodium excreted, but the effect was weaker on the experimental side than on contralateral side. With $0.1\;{\mu}g/kg/min$., two cases among 6 experiments showed marked diuresis, two cases no apparent effect, and another two marked antidiuresis on the experimental kidney, whereas the contralateral kidney exhibited antidiuresis in all cases. Further reducing the dose unmasked the diuretic action on the ,experimental kidney. In another series, the effects of isoproterenol on the blood flow distribution within the kidney and on sodium concentration gradient within the kidney tissue were observed. $0.05\;{\mu}g/kg/min$ isoproterenol markedly increased the medullary plasma flow and slightly increased total renal plasma flow and glomerular filtration rate, along with concomitant increase in the amount of sodium excreted and osmolar clearance, and decrease in reabsorption of free water. Sodium concentration gradient markedly decreased in the experimental kidney, reaching 2/3 of the value observed in the contralateral kidney at the papilla. It is thus concluded that isoproterenol exerts a diuretic action, when infused directly into a renal artery, and the mechanism of the action rests on its hemodynamic action, substantiated as the increase in glomerular filtration and in the medullary blood flow, resulting in washout of hyperosmolality produced by the coutercurrent multiplier system.

• Journal of Ginseng Research
• /
• 제41권2호
• /
• pp.188-194
• /
• 2017

Background: Fermented black ginseng (FBG) is processed ginseng by the repeated heat treatment and fermentation of raw ginseng. The protective effect and mechanism of FBG on cisplatin-induced nephrotoxicity was investigated to evaluate its therapeutic potential. Methods: The free radical scavenging activity of FBG was measured using 1,1-diphenyl-2-picrylhydrazyl (DPPH). In addition, the protective effect against cisplatin-induced renal damage was tested in rats. FBG was orally administered every day at a dose of 150 mg/kg body weight for 10 d, and a single dose of cisplatin was administered intraperitoneally (7.5 mg/kg body weight) with 0.9% saline on the $4^{th}$ d. Results: The DPPH radical-scavenging activity of FBG ($IC_{50}=384{\mu}g/mL$) was stronger than that of raw ginseng. The improved DPPH radical-scavenging activity was mediated by the generation phenolic compounds. The decreased cell viability by cisplatin was recovered significantly after treatment with FBG in a dose-dependent manner. Then, the protective effect of FBG on cisplatin-induced oxidative renal damage was investigated in rats. The decreased creatinine clearance levels, which are a reliable marker for renal dysfunction in cisplatin-treated rats, were reduced to the normal level after the administration of FBG. Moreover, FBG showed protective effects against cisplatin-induced oxidative renal damage in rats through the inhibition of $NF-{\kappa}B/p65$, COX-2, and caspase-3 activation. Conclusion: These results collectively show that the therapeutic evidence for FBG ameliorates the nephrotoxicity via regulating oxidative stress, inflammation, and apoptosis.

• 대한한의학회지
• /
• 제19권1호
• /
• pp.349-357
• /
• 1998

The aim of this experiments was to investigate the effect of Semen Plantaginis extract on. the renal function, plasma renin activity, plasma levels of atrial natriuretic peptide and aldosterone in rats. The results obtained were as follows: 1. Water balance decreased significantly after the administration of Semen Plantaginis extract. 2. Urine volume increased significantly after the administration of Semen Plantaginis extract, 0.1ml/200g. 3. Urinary excretion of sodium decreased significantly after the administration of Semen Plantaginis extract, 0.1 ml/200 g, 2week. 4. Urinary excretion of potassium decreased significantly after the administration of Semen Plantaginis extract, 0.1 ml/200 g, 2week. 5. Urinary excretion of chloride decreased significantly after the administration of Semen Plantaginis extract. 6. Free water clearance increased significantly after the administration of Semen Plantaginis extract 0.1 ml/200 g, 2 week and 0.2 ml/200 g. 7. Urinary excretion of creatinine increased significantly after the administration of Semen Plantaginis extract. 8. Plasma renin activity decreased significantly after the administration of Semen Plantaginis extract, 0.2 ml/200 g. 9. Plasma levels of aldosterone decreased significantly after the administration of Semen Plantaginis extract. 10. Plasma levels of atrial natriuretic peptide (ANP) decreased significantly after the administration of Semen Plantaginis extract.

• 동의생리병리학회지
• /
• 제20권1호
• /
• pp.181-186
• /
• 2006

A pharmacological inhibition of nitric oxide synthase (NOS) in rats produces vasoconstriction, renal dysfunction, and hypertension. The present study was aimed at investigating whether the methanol extract of Serous commixta cortex (MSC) ameliorates $N^G$-nitro-L-arginine methylester (L-NAME) induced hypertension in rats. Treatment of rats with L-NAME (10 mg/kg/day in drinking water, 5 weeks) caused a sustained increase in systolic blood pressure (SBP). Administration of MSC (100 or 200 mg/kg/day, p.o) significantly lowered the SBP in the L-NAME-treated rats and this effect was maintained throughout the whole experimental period. Moreover, ecNOS expression in aorta and kidney tissue from L-NAME treated rats was significantly restored dy administration of MSC. Furthermore, the impairment of acetylcholine (ACh)-induced relaxation of aortic rings in the L-NAME treated rats was reversed dy administering of MSC. The renal functional parameters including urinary volume, sodium excretion, and creatinine clearance (Ccr) were also restored by administering MSC. Taken together, the present study suggeststhat MSC prevents the increase in SBP in rats with L-NAME-induced hypertension, which may result from the up-regulation of the vascular and renal ecNOS/No system.

• Clinical and Experimental Pediatrics
• /
• 제50권11호
• /
• pp.1104-1109
• /
• 2007

목 적 : MPGN은 소아에서는 11년 이내에, 성인에서는 8년 이내에 50%가 사망하게 되는 불량한 예후를 지닌 만성신장병중의 하나이다. 한국에서는 1998년부터 학교집단뇨검사가 법으로 제정되어 시행되었다. 우리는 학교집단뇨검사를 통해 진단된 무증상의 막증식성 사구체신염과 증상이 있는 막증식성 사구체신염의 예후를 비교하였다. 방 법 : 1996년 1월부터 2005년 12월까지 본원 소아과에 입원하여 경피적 신생검 시행 후 병리조직학적 검사상 제 1형 MPGN으로 진단 받고 스테로이드 단독 또는 cyclosporine과 함께 또는 스테로이드만 단독으로 투여받고 있는 환자 18명의 특징과 예후를 분석하였다. 추적 관찰된 기간은 평균 6.3년이었다. 18명의 환자중에 7명의 환자는 학교집단뇨검사를 통해 진단된 환자이고, 11명의 환자는 진단당시 신증후군이나 급성신염, 육안적 혈뇨를 보이는 환자들이었다. 결 과 : 학교집단뇨검사로 진단된 환자들 가운데 남자는 4명이고, 여자는 3명이었다. 이들의 평균나이는 12.6세였다. 학교집단뇨검사에서 4명의 환자는 혈뇨와 단백뇨를 보였고, 1명의 환자는 단백뇨만 보였으며, 2명의 환자는 혈뇨만 보였다. 이들 가운데 3명이 진단 당시 혈청보체치가 감소하여 있었다. Methylprednisolone 충격요법 후에 5명의 환자(72%)가 혈뇨나 단백뇨없이 관해하였으며, 신기능이 저하된 환자는 한명도 없었다. 학교집단뇨검사를 받지 않은 그룹 가운데 남자는 9명이었고, 여자는 2명이었다. 이들의 평균나이는 14.4세였다. 진단 당시 7명의 환자는 신증후군 소견을 보였고, 3명의 환자는 육안적 혈뇨를 보였고, 1명의 환자는 현미경적 혈뇨와 함께 급성신부전의 소견을 보였다. 이들 가운데 진단 당시 혈청보체치가 감소하여 있던 환자는 5명이었다. 그리고 4명의 환자(36%)가 관해를 이루었으며, 1명의 환자는 복막투석을 필요로 하였으며, 3명의 환자에서 신기능이 저하되었으며 이 중 1명의 복막 투석을 필요로 하였다. 결 론 : 위의 사실들을 확실히 하기 위해서는 장기간의 연구가 필요하겠지만, 우리의 연구는 학교집단뇨검사를 통한 조기진단과 스테로이드 치료가 1형 막증식성사구체신염 환아의 예후에 도움이 될 것임을 보여주었다.

• Journal of Yeungnam Medical Science
• /
• 제11권1호
• /
• pp.101-108
• /
• 1994

저자들은 Gates의 방법으로 구한 사구체 여과율과 신장스캔 시행중에서 얻을 수 있는 relative intensity of peak renal to peak aortic activity (pK/pA), time between aortic and kidney peak(A-K) 및 parenchymal renal activity at 25 min compared to peak kidney activity(25K/pK)등의 지표들이 신기능 평가에 유용하게 사용될 수 있는지를 알기 위하여 정상 및 신장질환 환자등 57명을 대상으로 Gates의 방법을 시행하여 사구체 여과율을 구하고 이를 크레아티닌 청소율과 그리고 A-K, pK/pA및 25K/pK등의 지표들과 비교분석하여 다음과 같은 성적을 얻었다. $^{99m}Tc$-DTPA를 이용한 사구체 여과율과 크레아티닌 청소율 사이에는 유의한 상관관계가 있었다.(r=0.9) A-K, pK/pA 및 25K/pK 등과 $^{99m}Tc$-DTPA를 이용한 사구체 여과율 사이에는 각각 유의한 상관 관계가 있었다.(r=-0.42, 0.66, -0.48) 이상의 결과로 Gates의 방법을 이용한 사구체 여과율의 측정은 통상적인 신장 스캔과 함께 시행함으로서 환자에게 별다른 고통을 주지 않으면서 간편하게 시행할 수 있는 검사로써 임상에 유용하게 사용할 수 있을 것으로 생각되며 그리고 A-K, pK/pA 및 25K/pK등의 지표들도 신기능의 평가에 사구체 여과율과 함께 이용할 수 있을 것으로 사료된다.