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http://dx.doi.org/10.1016/j.jgr.2016.03.001

Preventive effect of fermented black ginseng against cisplatin-induced nephrotoxicity in rats  

Jung, Kiwon (College of Pharmacy, CHA University)
An, Jun Min (GINSENG BY PHARM Co., Ltd.)
Eom, Dae-Woon (Department of Pathology, University of Ulsan College of Medicine, Gangneung Asan Hospital)
Kang, Ki Sung (College of Korean Medicine, Gachon University)
Kim, Su-Nam (Natural Products Research Institute, Korea Institute of Science and Technology)
Publication Information
Journal of Ginseng Research / v.41, no.2, 2017 , pp. 188-194 More about this Journal
Abstract
Background: Fermented black ginseng (FBG) is processed ginseng by the repeated heat treatment and fermentation of raw ginseng. The protective effect and mechanism of FBG on cisplatin-induced nephrotoxicity was investigated to evaluate its therapeutic potential. Methods: The free radical scavenging activity of FBG was measured using 1,1-diphenyl-2-picrylhydrazyl (DPPH). In addition, the protective effect against cisplatin-induced renal damage was tested in rats. FBG was orally administered every day at a dose of 150 mg/kg body weight for 10 d, and a single dose of cisplatin was administered intraperitoneally (7.5 mg/kg body weight) with 0.9% saline on the $4^{th}$ d. Results: The DPPH radical-scavenging activity of FBG ($IC_{50}=384{\mu}g/mL$) was stronger than that of raw ginseng. The improved DPPH radical-scavenging activity was mediated by the generation phenolic compounds. The decreased cell viability by cisplatin was recovered significantly after treatment with FBG in a dose-dependent manner. Then, the protective effect of FBG on cisplatin-induced oxidative renal damage was investigated in rats. The decreased creatinine clearance levels, which are a reliable marker for renal dysfunction in cisplatin-treated rats, were reduced to the normal level after the administration of FBG. Moreover, FBG showed protective effects against cisplatin-induced oxidative renal damage in rats through the inhibition of $NF-{\kappa}B/p65$, COX-2, and caspase-3 activation. Conclusion: These results collectively show that the therapeutic evidence for FBG ameliorates the nephrotoxicity via regulating oxidative stress, inflammation, and apoptosis.
Keywords
cisplatin; fermented black ginseng; oxidative stress; Panax ginseng; renal damage;
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Times Cited By KSCI : 5  (Citation Analysis)
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