• Animal Bioscience
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• v.34 no.7
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• pp.1105-1115
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• 2021

Objective: To conserve and utilize the genetic resources of a traditional Chinese indigenous pig breed, Liangshan pig, we assessed the genetic diversity, genetic structure, and genetic distance in this study. Methods: We used 50K single nucleotide polymorphism (SNP) chip for SNP detection of 139 individuals in the Liangshan Pig Conservation Farm. Results: The genetically closed conserved population consisted of five overlapping generations, and the total effective content of the population (Ne) was 15. The whole population was divided into five boar families and one non-boar family. Among them, the effective size of each generation subpopulation continuously decreased. However, the proportion of polymorphic markers (P_N) first decreased and then increased. The average genetic distance of these 139 Liangshan pigs was 0.2823±0.0259, and the average genetic distance of the 14 boars was 0.2723±0.0384. Thus, it can be deduced that the genetic distance changed from generation to generation. In the conserved population, 983 runs of homozygosity (ROH) were detected, and the majority of ROH (80%) were within 100 Mb. The inbreeding coefficient calculated based on ROH showed an average value of 0.026 for the whole population. In addition, the inbreeding coefficient of each generation subpopulation initially increased and then decreased. In the pedigree of the whole conserved population, the error rate of paternal information was more than 11.35% while the maternal information was more than 2.13%. Conclusion: This molecular study of the population genetic structure of Liangshan pig showed loss of genetic diversity during the closed cross-generation reproduction process. It is necessary to improve the mating plan or introduce new outside blood to ensure long-term preservation of Liangshan pig.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.15 no.5
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• pp.1666-1689
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• 2021

The red line of Permanent Basic Farmland is the most important part in the "three-line" demarcation of China's national territorial development plan. The scientific and reasonable delineation of the red line is a major strategic measure being taken by China to improve its ability to safeguard the practical interests of farmers and guarantee national food security. The delineation of Permanent Basic Farmland zoning (DPBFZ) is essentially a multi-objective optimization problem. However, the traditional method of demarcation does not take into account the synergistic development goals of conservation of cultivated land utilization, ecological conservation, or urban expansion. Therefore, this research introduces the idea of artificial immune optimization and proposes a multi-objective model of DPBFZ red line delineation based on a clone selection algorithm. This research proposes an objective functional system consisting of these three sub-objectives: optimal quality of cropland, spatially concentrated distribution, and stability of cropland. It also takes into consideration constraints such as the red line of ecological protection, topography, and space for major development projects. The mathematical formal expressions for the objectives and constraints are given in the paper, and a multi-objective optimal decision model with multiple constraints for the DPBFZ problem is constructed based on the clone selection algorithm. An antibody coding scheme was designed according to the spatial pattern of DPBFZ zoning. In addition, the antibody-antigen affinity function, the clone mechanism, and mutation strategy were constructed and improved to solve the DPBFZ problem with a spatial optimization feature. Finally, Tongxu County in Henan province was selected as the study area, and a controlled experiment was set up according to different target preferences. The results show that the model proposed in this paper is operational in the work of delineating DPBFZ. It not only avoids the adverse effects of subjective factors in the delineation process but also provides multiple scenarios DPBFZ layouts for decision makers by adjusting the weighting of the objective function.

• Journal of Microbiology and Biotechnology
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• v.31 no.2
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• pp.207-216
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• 2021

Supplement of high-protein food plays an important role in improving the symptoms of malnutrition and the immune capacity of the body, but the association of high-protein diet and gut microbiota remained unaddressed. Here, we systematically analyzed the internal organs and gut microbiota in C57(WT) or PD-1H-depleted (KO) mice (T cells were activated) fed with pupae or feed for six weeks. We observed that the body weight gain in the mice fed with pupae increased less significantly than that of the feed group, while the villi and small intestine lengths in the pupa group were reduced compared with that of mice given feed. However, the average body weight of the KO mice increased compared with that of the WT mice fed with pupae or feed. Pupae increased the concentration of blood glucose in WT, but not in KO mice. Moreover, in the feed group, there was no difference in the weight of the internal organs between the WT and KO mice, but in the pupae-fed group, liver weight was decreased and spleen weight was increased compared with that of KO mice. The amounts/plural/amounts of Melainabacteria, Chloroflexi, and Armatimonadetes were specifically upregulated by pupae, and this upregulation was weakened or eliminated by PD-1H depletion. Some bacteria with high abundance in the feed-fed KO mice, such as Deferribacteres, Melainabacteria, Acidobacteria, Bacteroidetes, Spirochaetes and Verrucomicrobia, were decreased in pupae-fed KO mice, and Proteobacteria and Deinococcus were specifically enriched in pupae-fed KO mice. Bacteroidetes, Firmicutes and Akkermansia were associated with weight loss in the pupae-fed group while Lachnospiraceae and Anaerobiospirillum were related glucose metabolism and energy consumption. Based on high-throughput sequencing, we discovered that some gut bacteria specifically regulated the metabolism of a high-protein diet, and PD-1H deficiency improved life quality and sustained blood glucose. Moreover, PD-1H responses to high-protein diet through modulating the type and quantity of gut bacteria. These findings provide evidence about the association among gut microbiota, T cell activation (for PD-1H depletion) and high-protein diet metabolism, have important theoretical significance for nutrition and health research.

• Journal of Korean Society of Rural Planning
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• v.27 no.1
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• pp.103-116
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• 2021

The purpose of this study is to analyze and diagnose the actual state of extinction risk in rural villages by analyzing time series population characteristics and extinction risk index of rural villages(Haengjeong-ri). As a research method, a time series analysis was performed on the 'population over 65' and the 'female population aged 20 to 39' related to the local extinction index. In addition, the disappearance of Haengjeong-ri village was analyzed using the existing local extinction index. For the study, Haengjeong-ri village-level GIS spatial data was constructed, and population attribute data in 2010, 2015, and 2020 were constructed. In order to derive implications, the change of the 'high risk of extinction 'village in the 2020 local extinction index, the distribution of the population of 'high risk of extinction' villages by three years, the population analysis of 'high risk of extinction' villages continuously from 2010 to 2020, and 'high risk of extinction' An analysis of the increase and decrease of the population of the village and the change of the local extinction index of Haengjeong-ri, where the city, county, and towns are located were analyzed. As a result of the analysis of this study, it was found that the number of villages with an aging of more than 50% increased by 2 to 3 times over 10 years. As a result of the analysis of the local extinction index, in 2010, the southwestern and central regions of South Chungcheongnam-do were high-risk extinction areas, but in 2020, all cities except Cheonan and Asan were converted to high risk extinction areas. Research has shown that Chungnam is facing a serious aging and village extinction crisis, and since the disappearance of villages gradually spreads, an initial response policy is needed. This result will be the same not only in Chungcheongnam-do but also in other rural villages. Therefore, in the case of rural villages, it is necessary to analyze and diagnose the Haengjeong-ri village unit, not the Eup-Myeon unit, and a response policy through diagnosis must be prepared urgently.

• Journal of Korea Entertainment Industry Association
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• v.14 no.2
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• pp.155-164
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• 2020

This study identifies the relationship between selection value, existence value, heritage value, pride, and show on satisfaction, and suggests cultural marketing and cultural policy suggestions for Jikji cultural assets to activate Jikji as tourism cultural assets. Was intended. Therefore, the implications of this study are as follows. First, Jikji cultural property officials should develop tourism products that can mix Jikji cultural properties with the image and attractiveness of Jikji cultural properties. In addition, it is necessary to pay attention to education and public relations by city and county in providing local information, prices, and services for tourists to increase the satisfaction of tourists. Second, Jikji cultural property officials should suggest ways to create differentiating elements from tourism of other cultural properties. By emphasizing the existence, the existence value of Jikji cultural property should be increased. Third, Jikji cultural property officials should emphasize that Jikji tourism is more valuable as cultural heritage than now, and develop unique killer contents that can be boasted to others in tourism and present it to tourists. Fourth, Jikji cultural property officials should prepare a plan for local residents to recognize how excellent cultural heritage is. It should also be recognized that Jikji cultural property has high added value as a tourist factor. Lastly, Jikji cultural property officials should promote various jikji projects to local residents and tourists to increase their pride and awareness that Jikji cultural property exists in a certain area.

• Journal of the Korean Association of Geographic Information Studies
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• v.25 no.4
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• pp.1-18
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• 2022

Currently, the Korea Meteorological Administration evaluates the meteorological drought by region using SPI6(standardized precipitation index 6), which is a 6-month cumulative precipitation standard. However, SPI is an index calculated only in consideration of precipitation at 69 weather stations, and the drought phenomenon that appears for complex reasons cannot be accurately determined. Therefore, the purpose of this study is to calculate and compare SPI considering only precipitation and SDCI (Scaled Drought Condition Index) considering precipitation, vegetation index, and temperature in Gyeonggi. In addition, the advantages and disadvantages of the station data-based drought index and the satellite image-based drought index were identified by using results calculated through the comparison of SPI and SDCI. MODIS(MODerate resolution Imaging Spectroradiometer) satellite image data, ASOS(Automated Synoptic Observing System) data, and kriging were used to calculate SDCI. For the duration of precipitation, SDCI1, SDCI3, and SDCI6 were calculated by applying 1-month, 3-month, and 6-month respectively to the 8 points in 2014. As a result of calculating the SDCI, unlike the SPI, drought patterns began to appear about 2-month ago, and drought by city and county in Gyeonggi was well revealed. Through this, it was found that the combination of satellite image data and station data increased efficiency in the pattern of drought index change, and increased the possibility of drought prediction in wet areas along with existing dry areas.

• Journal of Korean Society of Forest Science
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• v.111 no.4
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• pp.482-489
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• 2022

This study aimed to determine the optimal planting density of Fraxinus rhynchophylla assessed from the early growth performance at various planting densities over the 7-year period after planting. The study site was in Pyeongchang County, South Korea, and seedlings of 2-year-old (bare-root seedlings) F. rhynchophylla were planted at four densities (3,000, 5,000, 7,000, and 10,000 trees ha^-1) in March 2015. The survival rate, root-collar diameter (RCD), and height (H) were measured from 2015 to 2021, and the H/D (H/RCD) ratio and stem volume were calculated. The survival rate (84-97%) and H/D ratio (54.5-59.2%) were not affected by the planting density during the study period, but the RCD, H, and stem volume were significantly higher for 7,000 trees ha^-1 than for other planting densities. Especially, the stem volume (cm³ tree^-1) at 7 years after planting was highest for 7,000 trees ha^-1 (1,356.1), followed by 10,000 trees ha^-1 (958.6), 5,000 trees ha^-1 (773.0), and 3,000 trees ha^-1 (579.5). As the planting density increased, F. rhynchophylla seedlings showed initial rapid growth due to light competition, but relatively low growth at excessive planting densities. In the future, use of a suitable planting density considering planting costs should provide outstanding growth performance of F. rhynchophylla on plantations.

• Journal of Veterinary Science
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• v.21 no.5
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• pp.80.1-80.13
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• 2020

Background: In suckling piglets, transmissible gastroenteritis virus (TGEV) causes lethal diarrhea accompanied by high infection and mortality rates, leading to considerable economic losses. This study explored methods of preventing or inhibiting their production. Bovine antimicrobial peptide-13 (APB-13) has antibacterial, antiviral, and immune functions. Objectives: This study analyzed the efficacy of APB-13 against TGEV through in vivo and in vitro experiments. Methods: The effects of APB-13 toxicity and virus inhibition rate on swine testicular (ST) cells were detected using 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT). The impact of APB-13 on virus replication was examined through the 50% tissue culture infective dose (TCID₅₀). The mRNA and protein levels were investigated by real-time quantitative polymerase chain reaction and western blot (WB). Tissue sections were used to detect intestinal morphological development. Results: The safe and effective concentration range of APB-13 on ST cells ranged from 0 to 62.5 ㎍/mL, and the highest viral inhibitory rate of APB-13 was 74.1%. The log₁₀TCID₅₀ of 62.5 ㎍/mL APB-13 was 3.63 lower than that of the virus control. The mRNA and protein expression at 62.5 ㎍/mL APB-13 was significantly lower than that of the virus control at 24 hpi. Piglets in the APB-13 group showed significantly lower viral shedding than that in the virus control group, and the pathological tissue sections of the jejunum morphology revealed significant differences between the groups. Conclusions: APB-13 exhibited good antiviral effects on TGEV in vivo and in vitro.

• Journal of the Korean Society for Library and Information Science
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• v.56 no.1
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• pp.5-25
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• 2022

In most countries, public libraries are knowledge information centers, complex cultural spaces, lifelong learning facilities, and third places for local residents. The social homage spoken on the premise of this identity and socio-cultural role is the knowledge and information agora, cultural infrastructure, living room in the city, and the university of the people. However, if there is a significant gap between public libraries regardless of country or region, it will inevitably lead to information gaps, regional gaps, and cultural inequality and cultural welfare gaps among local residents. This study is regarded as the essence of regional cultural facilities and premised that the regional gap in public libraries is a factor of regional cultural gaps. Based on this premise, the gap between the location quotient, input indicators, and output indicators of public libraries of regional governments in Korea and basic local governments in the Gyeongbuk region was analyzed. And this study derived core elements through correlation and regression analysis of input and output indicators of all public libraries nationwide, developed a management model for each indicator, and suggested a plan to resolve the relative gap for public libraries by city and county in Gyeongbuk.

• The Journal of Korean Medicine
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• v.43 no.1
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• pp.6-17
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• 2022

Objectives: This study describes the implementation of a training course that aimed to increase the competency of undergraduate students of Korean medicine at a single college to obtain informed consent and perform bee venom acupuncture using an objective structured clinical examination (OSCE). Methods: We developed and implemented a short training course for 111 fourth-year students of Korean medicine during their clinical rotation at the Department of Acupuncture and Moxibustion Medicine. Lectures were conducted on the effects of and hypersensitivity reactions to bee venom acupuncture, obtaining informed consent, performing skin test and bee venom acupuncture. Due to the COVID-19 pandemic, some students were offered online lectures, and some could not experience the performance of OSCE in person. A survey with 16 closed and three open questions was used to investigate self-efficacy, opinions about the online lectures, and the OSCE. Results: Out of 111 questionnaires, 104 were completed (94%). Students were generally satisfied with the online lectures and OSCE. For the subjective questionnaire about what was helpful in the training of bee venom acupuncture techniques and OSCE, the authenticity of the teaching method was recorded the highest. Conclusions: This study showed that online lectures were effective in teaching clinical skills of Korean Medicine. It is necessary to produce standard educational materials for clinical skills of Korean Medicine, and studies to analyze the reliability and validity of OSCE are needed.