• Applied Biological Chemistry
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• v.29 no.3
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• pp.279-287
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• 1986

The changes in peroxidase activity and its isozyme pattern in the different parts of mung bean sprout were investigated; The enzyme activity in cotyledon and root showed a tendency to increase at an early stage and then decreased gradually as germination continued. However, the crude homogenate of epicotyl and hypocotyl showed a continuous decline in the enzyme activity. In particular, the enzyme activity of the root was $1.5{\sim}3.5$ times higher than that of other parts. Gel electrophoresis of the crude homogenate revealed that the number of isozyme in every part of the mung bean sprout increase during germination up to 6th days. Two isozymes from root were partially purified by ammonium sulfate fractionation, gel filtration by Sephadex G-75 and DEAE cellulose column chromatography. One of the isozymes (A) was purified 16-fold by the present procedure, but the purity of the other isosyme (B) was not increased , significantly. Isozyme A was the most active at $65^{\circ}C$ and isozyme B at $70^{\circ}C$, while both isozyme (A, B) have a optimal pH of 5.6. The Km values of isozyme A and B for 0-dianisidine as a hydrogen donor determined to be 0.071 mM and 0.052mM, respectively, and those for isozyme A and B using $H_2O_2$ as a hydrogen acceptor were 0.28mM and 0.23mM, respectively.

• Korean Journal of Soil Science and Fertilizer
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• v.46 no.1
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• pp.49-52
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• 2013

Seed coat of hairy vetch is very thick and hard, and difficult to absorb water during germination. It requires much time that cotyledon comes out from seed coat. Therefore this experiment was carried out to increase the germination rate by enhancing water absorption through water-soaking of seed, chemical scarification by sulfuric acid, and mechanical scarification on seed coat. Water-soaking for 5 hours seemed to be highly effective in enhancing germination rate. Although not significant, water-soaking resulted in increase of germination rate by 9%. Effect of soaking temperature was not significant, but early germination rate was increased to be 5.0%, 31.7% and 48.3% at $10^{\circ}C$, $20^{\circ}C$ and $30^{\circ}C$, respectively. Mechanical scarification of seed coat led to a germination rate of 97% whereas intact seed showed that of 65%. Chemical scarification by sulfuric acid for 10min, 20min and 30min resulted in a germination rate of 76.7%, 74.7% and 96.0% respectively. It is clear that scarification increased germination rate.

• Korean Journal of Food Science and Technology
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• v.35 no.4
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• pp.568-575
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• 2003

Selection of high-quality varieties of sprout bean and bean sprout containing high levels of total isoflavones was performed by high performance liquid chromatography. The range and mean of total isoflavone contents of sixty six varieties of sprout beans were $247{\sim}2,256$ and $1209{\pm}470\;mg/kg$, respectively, with KLG10618, KLG11118, KLG10600, KLG10022, KLG1085 and sohokong containing the highest amounts of isoflavone among the samples. Medium-sized bean variety with green seed-coat color contained highest amount of total isoflavones among samples. The range and mean of total isoflavone contents of thirty varieties of bean sprouts were $768{\sim}3,343$ and $1,898{\pm}577\;mg/kg$, respectively, with soho bean sprout containing the highest total isoflavone content (3,343 mg/kg, dry basis). Total isoflavone contents of bean sprouts increased gradually during cultivation period, reaching maximum level on the 5th day of cultivation, and were the highest in the order of roots, cotyledon, and hypocotyl.

• Korean Journal of Food Science and Technology
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• v.35 no.4
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• pp.556-560
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• 2003

It was determined whether the sandwich ELISA using specific anti-CP4 EPSPS polyclonal and monoclonal antibodies, developed in the previous study, could be applied to detect GM soybean or not. The soybeans (47 imported and 20 domestic soybeans) were analyzed by a sandwich ELISA. The results of imported soybeans were divided into two groups which were high contents $(39.1{\pm}13.5\;{\mu}g/g,\;n=33)$ and low contents of CP4 EPSPS $(2.6{\pm}1.2\;{\mu}g/g,\;n=14)$. The ratio of GM in imported soybeans was about 70.2%. One the other hand, the contents of CP4 EPSPS in domestic soybeans was very low $(0.9{\pm}0.5\;{\mu}g/g,\;n=20)$ which determined to be non-GM soybeans. In case of soybean sprouts, the contents of CP4 EPSPS in soybean sprouts were different between GM and non-GM soybean sprout. The CP4 EPSPS in cotyledon of GM soybeans sprout was higher than that in root hair. The contents of CP4 EPSPS in soybeans sprout of domestic soybeans were very low. Thus, it was possible to determine that the soybeans sprout was made of GM or non-GM soybeans. Also, PCR experiment showed that the sandwich ELISA was accurate to distinguish the soybeans to be GM or non-GM. These results showed the sandwich ELISA could determine the soybeans were GM or non-GM, rapidly and simply.

• Molecules and Cells
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• v.40 no.3
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• pp.230-242
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• 2017

In the Arabidopsis genome, approximately 80 MAP3Ks (mitogen-activated protein kinase kinase kinases) have been identified. However, only a few of them have been characterized, and the functions of most MAP3Ks are largely unknown. In this paper, we report the function of MAP3K16 and several other MAP3Ks, MAP3K14/15/17/18, whose expression is salt-inducible. We prepared MAP3K16 overexpression (OX) lines and analyzed their phenotypes. The result showed that the transgenic plants were ABA-insensitive during seed germination and cotyledon greening stage but their root growth was ABA-hypersensitive. The OX lines were more susceptible to water-deficit condition at later growth stage in soil. A MAP3K16 knockout (KO) line, on the other hand, exhibited opposite phenotypes. In similar transgenic analyses, we found that MAP3K14/15/17/18 OX and KO lines displayed similar phenotypes to those of MA3K16, suggesting the functional redundancy among them. MAP3K16 possesses in vitro kinase activity, and we carried out two-hybrid analyses to identify MAP3K16 substrates. Our results indicate that MAP3K16 interacts with MKK3 and the negative regulator of ABA response, ABR1, in yeast. Furthermore, MAP3K16 recombinant protein could phosphorylate MKK3 and ABR1, suggesting that they might be MAP3K16 substrates. Collectively, our results demonstrate that MAP3K16 and MAP3K14/15/17/18 are involved in ABA response, playing negative or positive roles depending on developmental stage and that MAP3K16 may function via MKK3 and ABR1.

• Korean Journal of Medicinal Crop Science
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• v.2 no.1
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• pp.81-85
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• 1994

This experiment was carried out to investigate the fertilization and the size of mature female and male gametophytic parts of Pulsatilla koreana after artificial pollination. The size of pollen is $26.5{\mu}m$ at the time of anther dehiscence, that is, about $3{\sim}4$ days after pollination. Synergid nucleus, egg nucleus, and polar nucleus are 10.0, 15.0, and $32.5{\mu}m$ respectively at the time of completing egg apparatus formation, that is, about 2 days after pollination. Poller tubes germinate on stigma about 10 hours, passing lower part of style about 30 hours, penetrating into micropyle about 35 hours after pollination. Sperm nucleus penetrates into polar nucleus about 40 hours and egg cell about 48 hours after pollination. But, there seems to be different among the individuals. Multinuclei and multinucleoli are formed in egg cell, synergid, and polar nucleus about the time of fertilization. Proembryo is formed about 4 days, being changed to large globular form about $6{\sim}8$ days after pollination. Endosperm nuclei divide into free nuclei after fertilization and change to cotyledon in gymnosperm. There seems to be same phenomena in Pulsatilla koreana.

• Weed & Turfgrass Science
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• v.4 no.2
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• pp.118-123
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• 2015

Metagenomics is a powerful tool to isolate novel biocatalyst and biomolecules directly from the environmental DNA libraries. Since the metagenomics approach bypasses cultivation of microorganisms, un-cultured microorganisms that are majority of exists can be the richest reservoir for natural products discovery. To discover novel herbicidal substances from soil metagenome, we established three easy, simple and effective high throughput screening methods such as cucumber cotyledon leaf disc assay, microalgae assay and seed germination assay. Employing the methods, we isolated two active single clones (9-G1 and 9-G12) expressing herbicidal activity which whitened leaf discs, inhibited growth of microalgae and inhibited root growth of germinated Arabidopsis seeds. Spraying butanol fraction of the isolated active clones' culture broth led to growth retardation or desiccation of Digitalia sanguinalis (L) Scop. in vivo. These results represent that the screening methods established in this study are useful to screen herbicidal substances from metagenome libraries. Further identifying molecular structure of the herbicidal active substances and analyzing gene clusters encoding synthesis systems for the active substances are in progress.

• Journal of the Korean Society of Food Science and Nutrition
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• v.24 no.6
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• pp.937-942
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• 1995

Cutting force, degree of cooking, cooking loss and absorbances of cooking solution of three cultivars of Korean kidney beans, Pink(PKB), Red(RKD) and White(WKB) were compared. The cutting force of raw kidney bean was 23,500~27,000g. The optimum soaking conditions to give the same cutting force of beans were 10hr at $10^{\circ}C,\;8hr\;at\;20^{\circ}C,\;6hr\;at\;30^{\circ}C\;and\;3hr\;at\;40^{\circ}C$. At optimum soaking conditions, the degree of cooking was determined by measuring the maximum cutting force of cotyledon. The terminal points of cooking at $98^{\circ}C$ were 23min for PKB, 25min for RKB and 27min for WKB. Cooking loss of kidney beans during cooking were 3.4~5.4%. Absorbances of cooking solution showed a similar pattern in all samples, except PKB soaked at $10^{\circ}C$ for 10hr.

• Journal of Korean Society of Forest Science
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• v.99 no.4
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• pp.633-637
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• 2010

This study was conducted to evaluate various effects of kinds of culture medium, concentrations of abscisic acid (ABA) or /kinds of osmotica on maturation of somatic embryos (SEs) with four (LL-L, LL-K, LL-P and LL-N) embryogenic tissue lines (ETLs) in Japanese larch (Larix leptolepis). In comparison of two culture medium, the LL-P produced the highest number of the cotyledon-staged SE (134.9/90 mg tissue) in 1/2LM medium. In contrast, no SEs were obtained except the LL-P (32.9) in medium of BLG. Effects of two concentrations of ABA in the medium with four ETL for SEs maturation were also compared. In the test of 60 or 100 ${\mu}M$ ABA, the highest result was obtained in 60 ${\mu}M$ ABA (142.9). However, the influence of ABA had little on SEs production except the LL-N regardless of concentrations of ABA. In comparison of different kinds/concentrations of osmotica, the best response was obtained from the treatment of 0.2 M maltose, the LL-K (540.5). In conclusion, the effects of production of SEs were greatly rely on the ETLs, rather than kinds of medium, concentrations of ABA or osmotica which were used in maturation of SEs.

• Journal of Animal Science and Technology
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• v.46 no.5
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• pp.879-886
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• 2004

Effects of culture mediwn supplements and osmotic stress treatment on embryogenic callus induction and somatic embryogenesis were investigated in order to optimize tissue culture conditions of alfalfa(Medicago sativa L.). SH mediwn containing 5mgIL 2,4-D and 0.2mgIL kinetin was optimal for embryogenic callus induction from cotyledon tissue of alfalfa. Somatic embryos were formed when the embryogenic callus was cultured on SH mediwn supplemented with ImgIL 2,4-D and 2mgIL BA. Supplementation of 5mM L-proline and IgIL casein hydrolysate into the regeneration mediwn further increased plant regeneration frequency. Osmotic stress treatment of callus appeared to improve the frequency of somatic embryo formation, but the frequency of somatic embryo formation differed by the osmotic stress treatment using different osmotic stressors. The highest plant regeneration frequency of 30.7% was observed when embryogenic callus was treated with 0.7M sucrose for 18h. Efficient regeneration system established in this study will be useful for molecular breeding of alfalfa through genetic transformation.