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http://dx.doi.org/10.5187/JAST.2004.46.5.879

Efficient Plant Regeneration from Alfalfa Callus by Osmotic Stress Treatment  

Kim, J.S. (Division of Applied Life Science, Gyeongsang National University)
Lee, D.G. (Division of Applied Life Science, Gyeongsang National University)
Lee, S.H. (Division of Applied Life Science, Gyeongsang National University)
Woo, H.S. (Division of Applied Life Science, Gyeongsang National University)
Lee, B.H. (Division of Applied Life Science, Gyeongsang National University)
Publication Information
Journal of Animal Science and Technology / v.46, no.5, 2004 , pp. 879-886 More about this Journal
Abstract
Effects of culture mediwn supplements and osmotic stress treatment on embryogenic callus induction and somatic embryogenesis were investigated in order to optimize tissue culture conditions of alfalfa(Medicago sativa L.). SH mediwn containing 5mgIL 2,4-D and 0.2mgIL kinetin was optimal for embryogenic callus induction from cotyledon tissue of alfalfa. Somatic embryos were formed when the embryogenic callus was cultured on SH mediwn supplemented with ImgIL 2,4-D and 2mgIL BA. Supplementation of 5mM L-proline and IgIL casein hydrolysate into the regeneration mediwn further increased plant regeneration frequency. Osmotic stress treatment of callus appeared to improve the frequency of somatic embryo formation, but the frequency of somatic embryo formation differed by the osmotic stress treatment using different osmotic stressors. The highest plant regeneration frequency of 30.7% was observed when embryogenic callus was treated with 0.7M sucrose for 18h. Efficient regeneration system established in this study will be useful for molecular breeding of alfalfa through genetic transformation.
Keywords
Alfalfa; Callus; Plant regeneration; Somatic embryogenesis;
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