• Title/Summary/Keyword: cortical surface

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Ascophyllum and Its Symbionts. VII. Three-way Interactions Among Ascophyllum nodosum (Phaeophyceae), Mycophycias ascophylli (Ascomycetes) and Vertebrata lanosa (Rhodophyta)

  • Garbary, David J.;Deckert, Ron J.;Hubbard, Charlene B.
    • ALGAE
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    • v.20 no.4
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    • pp.353-361
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    • 2005
  • Ascophyllum nodosum (L.) Le Jolis has a systemic infection with the ascomycete Mycophycias ascophylli (Cotton) Kohlmeyer and Volkmann-Kohlmeyer with which it establishese a mutualistic symbiosis. In addition, A. nodosum is the host for the obligate red algal epiphyte, Vertebrata lanosa (L.) Christensen. Using light and electron microscopy we describe morphological and cytochemical changes occurring as a consequence of rhizoid penetration of V. lanosa into cortical host tissue. Rhizoids induce localized cell necrosis based on physical damage during rhizoid penetration. Host cells adjacent to the rhizoid selectively undergo a hypersensitive reaction in which they become darkly pigmented and become foci for hyphal development. Light and electron microscopy show that M. ascophylli forms dense hyphal aggregations on the surface of the V. lanosa rhizoid and extensive endophytic hyphal growths in the rhizoid wall. This is the first morphological evidence of an interaction between M. ascophylli and V. lanosa. We speculate that M. ascophylli may be interacting with V. lanosa to limit tissue damage to their shared host. In addition, the fungus provides a potential pathway for the transfer of materials (e.g., nutrients and photosynthate) between the two phototrophs.

Surgery of Parasplenial Arteriovenous Malformation with Preservation of Vision - A Case Report - (부뇌량팽대 동정맥 기형의 수술에서 시야의 보존 - 증례보고 -)

  • Joo, Jin Yang;Ahn, Jung Yong
    • Journal of Korean Neurosurgical Society
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    • v.29 no.6
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    • pp.815-821
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    • 2000
  • Parasplenial arteriovenous malformations(AVMs) are rare vascular malformations which have distinct clinical and anatomical features. They are situated at the confluence of the hippocampus, isthmus of the cingulate gyrus and the gyrus occipitotemporalis medialis. These lesions are anterior to the calcarine sulcus and their apex extends towards the medial surface of the trigonum. Posterolaterally, these lesions are in close proximity to the visual cortex and optic radiation. The objectives in the surgery of parasplenial AVMs are complete resection of the lesions and preservation of vision. These objectives must be achieved with comprehensive understanding of the following anatomical features :1) the deep central location of the lesions within eloquent brain tissue ; 2) the lack of cortical representation of the AVMs that requires retraction of visual cortex ; 3) deep arterial supply ; 4) deep venous drainage ; 5) juxtaposition to the choroid plexus with which arterial supply and venous drainage are shared. A 16-year-old female student presented with intraventricular hemorrhage from a right parasplenial-subtrigonal AVM. The lesion, fed by posterior cerebral artery and drained into the vein of Galen, was successfully treated by the inter-hemispheric parietooccipital approach. To avoid visual field defect a small incision was made on precuneus anterior to the calcarine sulcus. In this report, the authors describe a surgical approach with special consideration on preservation of visual field.

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Morphological Redescriptions of Three Condylostoma Ciliates (Heterotrichida: Condylostomatidae) New to Korea

  • Kim, Ji-Hye;Jang, Seok-Won;Shin, Mann-Kyoon
    • Animal Systematics, Evolution and Diversity
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    • v.28 no.3
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    • pp.149-160
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    • 2012
  • Heterotrichous ciliates were isolated from marine and brackish habitats in Korea, and their morphology, infraciliature and morphometrical characters were investigated using silver impregnated methods. These three Condylostoma species were identified as Condylostoma curva Burkovsky, 1970, C. minutum Bullington, 1940 and C. spatiosum Ozaki and Yagiu in Yagiu, 1944. The species of Condylostoma based on morphology are highly variable in most of characters and distinguished as following. Condylostoma curva is oval to elongate ellipsoidal, size about $245{\times}100{\mu}m$ in vivo, and conspicuously dark greenish brown with cortical granules on their surface, buccal cavity relatively large about 40% of body length, 5-11 macronuclear nodules, 4-8 frontal cirri serially arranged and 22-38 somatic kineties. Condylostoma minutum is elongate ellipsoidal and the size about $310{\times}55{\mu}m$ in vivo, buccal field about 35% of body length, 12-20 macronuclear nodules, adoral zone consisted of 82-107 membranelles, large and long one frontal cirrus and 38-44 somatic kineties. Condylostoma spatiosum is large elongate ellipsoidal, size about $670{\times}105{\mu}m$ in vivo, buccal cavity about 25% of body length, 11-25 macronuclear nodules, 111-144 adoral membranelles, membrane-like 2 frontal cirri, 49-74 somatic kineties. These three Condylostoma species are described here for the first time in Korea.

Cell Division in the Absence of Mitosis: The Unusual Case of the Fucoid Ascophyllum nodosum (L.) Le Jolis (Phaeophyceae)

  • Garbary, David J.;Lawson, Greg;Clement, Kelly;Galway, Moira E.
    • ALGAE
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    • v.24 no.4
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    • pp.239-248
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    • 2009
  • Fluorescence and electron microscopy were used to examine epidermal shedding in the fucoid alga, Ascophyllum nodosum. Mature meristoderm cells are ca. 50-100 x 30-40 ${\mu}m$ and highly polarized, with a single nucleus and chloroplasts near the base of the cell. Nuclei in these cells undergo mitosis when they are dividing to form a new cortical cell towards the middle of the frond, or anticlinal divisions as part of frond elongation. However, cytokinesis also occurs regularly in these cells when a new periclinal wall is deposited at about 30% of the cell length from the apical end. The newly formed distal cells are anucleate and without chloroplasts. Following cytokinesis the tangential walls then break at the thinnest point. The whole process is synchronous in adjoining epidermal cells across large areas of the frond surface, and this layer dehisces from the thallus. This is the only known plant or algal system in which cytokinesis regularly occurs in the absence of mitosis. We consider this process a novel form of programmed cell death.

IMPLANT REHABILITATION IN THE UNFAVORABLE ALVEOLAR RIDGE (불량한 치조제에서의 임플랜트 시술증례)

  • Park, Jae-Bum;Ahn, Sang-Hun;Cheung, Soo-Il;Jo, Byung-Woan;Ahn, Jae-Jin
    • Maxillofacial Plastic and Reconstructive Surgery
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    • v.19 no.1
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    • pp.35-44
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    • 1997
  • The most critical factor in determining which type of implant to be used would be the available bone of the patient. Usually a minimum of 5mm in the bone width and 8mm in the bone height is necessary to ensure primary implant stability and maintain the integrity of bone contact surface. Placement of implant is limited by the several anatomic strutures such as maxillary sinus, floor of the nose, inferior alveolar neurovascular bundle and nasopalatine foramen, etc. When severe resorption of alveolar ridge is encountered, implant placement would be a problematic procedure. A number of techniques to improve the poor anatomic situations have been proposed. This article reports 4 cases of patients using surgical procedures such as blade implant technique, cortical split technique in the anterior maxillary area, sinus lifting and lateral repositioning of inferior alveolar nerve, We treated dental implant candidates with unfavorable alveolar ridge utilizing various surgical techniques, resulted in successful rehabilitation of edentulous ridge.

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Ultrastructure of Germ Cell during the Gametogenesis in Surf Clam, Spisula sachalinensis (북방대합 (Spisula sachalinensis) 생식세포의 미세구조)

  • LEE Jeong Yong;CHANG Yun Jeong;CHANG Young Jin
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.36 no.2
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    • pp.157-162
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    • 2003
  • Ultrastructure of germ cell during gametogenesis of surf clam, Spisula sachalinensis were investigated by electron microscopic observations. Oogonia are oval in shape and 5-6 ${\mu}m$ in diameter. They contain a large nucleus and have several mitochondria in the cytoplasm. Vitellogenic oocytes grew attached by a stalk to the germinal epithelium in the ovarian sac and their cytoplasm contained many yolk granules, lipid granules and mitochondria. Mature oocytes measuring 50-60 ${\mu}m$ in diameter have a nucleus containing an electron dense nucleolus, and a lots of yolk granules, lipid granules, mitochondria, rough endoplasmic reticulum and cortical granules were present in the cytoplasm. The surface of the plasma membrane in mature oocytes was formed of microvilli approximately 1.5 ${\mu}m$ long and enveloped in the vitelline layer. Spematogonia are oval in shape and about 5 ${\mu}m$ in diameter. Sprmatogonia develop into spermatocyte, spermatid and spermatozoon. The spermatozoon consisted of the head, midpiece and tail. The sperm head with diameter of about 1.5 ${\mu}m$ was ovoid, and contained the nucleus which consisted of acrosome. The four mitochondria encircled the centrosome in midpiece. The flagellum measuring about 40 ${\mu}m$ long had the classical 9+2 axoneme structure.

Regulation of DREAM Expression by Group I mGluR

  • Lee, Jin-U;Kim, In-Sook;Oh, So-Ra;Ko, Suk-Jin;Lim, Mi-Kyung;Kim, Dong-Goo;Kim, Chul-Hoon
    • The Korean Journal of Physiology and Pharmacology
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    • v.15 no.2
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    • pp.95-100
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    • 2011
  • DREAM (downstream regulatory element antagonistic modulator) is a calcium-binding protein that regulates dynorphin expression, promotes potassium channel surface expression, and enhances presenilin processing in an expression level-dependent manner. However, no molecular mechanism has yet explained how protein levels of DREAM are regulated. Here we identified group I mGluR (mGluR1/5) as a positive regulator of DREAM protein expression. Overexpression of mGluR1/5 increased the cellular level of DREAM. Up-regulation of DREAM resulted in increased DREAM protein in both the nucleus and cytoplasm, where the protein acts as a transcriptional repressor and a modulator of its interacting proteins, respectively. DHPG (3,5-dihydroxyphenylglycine), a group I mGluR agonist, also up-regulated DREAM expression in cortical neurons. These results suggest that group I mGluR is the first identified receptor that may regulate DREAM activity in neurons.

Fetal bladder outlet obstruction in a stillborn bovine fetus

  • Jeong, Won-Il;Lee, Cha-Soo;Chung, Jae-Yong;Jeong, Da-Hee;Do, Sun-Hee;Noh, Dong-Hyung;Oh, Won-Seok;Jeong, Kyu-Shik
    • Proceedings of the Korean Society of Veterinary Pathology Conference
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    • 2002.11a
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    • pp.134-134
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    • 2002
  • A stillborn bovine male fetus with abdominal distention, arthrogryposis and atresia ani was presented for diagnostic evaluation. At necropsy, this fetus had a large amount of ascites, urachal obstruction and marked bladder distention. The ventral surface of the bladder had ruptured and attached to the abdominal wall by fibrinous adhesions. There was bilateral hydronephrosis with moderate pelvic dilatation and cortical attenuation. The rectum was filled with meconium but the anus was imperforate. The right forelimb was contracted. The cause(s) of these abnormalities could not be determined; however, we believe that developmental abnormalities during embryogenesis may be the result of chromosomal abnormalities. This report is the first to report congenital urachal obstruction in this species.

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A Finite Element Analysis of Incisors with Different Material Combinations of a Post and a Core (기둥(Post)과 핵(Core)의 이종재료 조합에 의한 치아의 유한요소해석)

  • Kang, Min-Kyu;Tak, Seung-Min;Lee, Seok-Soon;Seo, Min-Seock;Kim, Hyo-Jin
    • Journal of the Korean Society for Precision Engineering
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    • v.28 no.4
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    • pp.474-481
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    • 2011
  • The purpose of this study was to investigate the effect of rigidity of post core systems on stress distribution by the finite element stress analysis method. Three-dimensional finite element models simulating an endodontically treated maxillary central incisor restored with a zirconia crown were prepared. Each model contained cortical bone, trabecular bone, periodontal ligament, 4mm apical root canal filling, and post-and-core. A 50N static occlusal load was applied to the palatal surface of the crown with a $60^{\circ}$ angle to the long axis of the tooth. And three parallel type post (zirconia, glass fiber and stainless steel) and two core (Paracore and Tetric ceram) materials were evaluated, respectively. The differences in stress transfer characteristics of the models were analyzed. von Mises stresses were chosen for presentation of results and maximum displacement and hydrostatic pressure were also calculated. For the Result of the research, the model applied glass fiber to post material has lowest von Mises stress and it is suitable for material of post core systems.

Notes on the Endothia Canker of Carpinus laxiflora and its Pathogenic Fungus, Endothia fluens Schw Shear et Stevens (서-나무 위축병(萎縮病)과 그 병원균(病原菌))

  • Kim, Kichung
    • Journal of Korean Society of Forest Science
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    • v.6 no.1
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    • pp.6-10
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    • 1967
  • In the present paper author investigated the symptom, pathogenic fungus and pathogenicity of Endothia canker of Carpinus laxiflora in Korea, and made clear the indistinct discription on its pathogen in the past. 1. The pathogen is identified as Endothia fluens (Schw.) Shear et Stevens. The discription is recorded as follows: Stromata cortical, erumpent, spherical or conical, outer yellowish-brown and inner yellowish, 0.5 to 2.5 mm in diameter; perithecia irregularly embeded in the bottom of stroma, 7 to 23 in a stroma usually spherical to elliptical or irregular, 235 to $370{\mu}$ in diameter, with black slender necks; each neck open the papilliate ostiole to the surface, about 250 to $400{\mu}$ in length; asci clavate or fusoid, colorless, 31.16 to 42.64 by 6.54 to $8.20{\mu}$ in size, average 37.02 by $6.84{\mu}$, with 8 ascospores in double line; ascospores elliptical, ovate or cylindrical, with rounded ends, hyaline, 1-septate, not constrict at the septum, 6.51 to 9.30 by 3.16 to $3.72{\mu}$, average 7.61 by $3.44{\mu}$ in size; pycnidia formed abundantly in stroma. spherical at first but later irregular large cavity by fussing each other; pycnospores oblong or rod-shaped, hyaline, non-septate, 3.8 by $1.9{\mu}$ in size; spore-horn formed abundantly under moist condition. 2. The pathogen is wound parasite invading the hosts through the wound. But after the infection is established, expanding the disease lesion is swiftly vigorus.

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