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http://dx.doi.org/10.5657/kfas.2003.36.2.157

Ultrastructure of Germ Cell during the Gametogenesis in Surf Clam, Spisula sachalinensis  

LEE Jeong Yong (Kangnung Hatchery, NFRDI)
CHANG Yun Jeong (Fisheries Management Division., Donghae, MOMAF)
CHANG Young Jin (Department of Aquaculture, Pukyong National University)
Publication Information
Korean Journal of Fisheries and Aquatic Sciences / v.36, no.2, 2003 , pp. 157-162 More about this Journal
Abstract
Ultrastructure of germ cell during gametogenesis of surf clam, Spisula sachalinensis were investigated by electron microscopic observations. Oogonia are oval in shape and 5-6 ${\mu}m$ in diameter. They contain a large nucleus and have several mitochondria in the cytoplasm. Vitellogenic oocytes grew attached by a stalk to the germinal epithelium in the ovarian sac and their cytoplasm contained many yolk granules, lipid granules and mitochondria. Mature oocytes measuring 50-60 ${\mu}m$ in diameter have a nucleus containing an electron dense nucleolus, and a lots of yolk granules, lipid granules, mitochondria, rough endoplasmic reticulum and cortical granules were present in the cytoplasm. The surface of the plasma membrane in mature oocytes was formed of microvilli approximately 1.5 ${\mu}m$ long and enveloped in the vitelline layer. Spematogonia are oval in shape and about 5 ${\mu}m$ in diameter. Sprmatogonia develop into spermatocyte, spermatid and spermatozoon. The spermatozoon consisted of the head, midpiece and tail. The sperm head with diameter of about 1.5 ${\mu}m$ was ovoid, and contained the nucleus which consisted of acrosome. The four mitochondria encircled the centrosome in midpiece. The flagellum measuring about 40 ${\mu}m$ long had the classical 9+2 axoneme structure.
Keywords
Surf clam; Spisula sachalinensis; Ultrastructure; Oogenesis; Spormatogenesis;
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