• Journal of the Society of Cosmetic Scientists of Korea
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• v.45 no.4
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• pp.373-380
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• 2019

There are many differences in tran-epidermal water loss (TEWL), skin water contents, and skin elasticity, etc between face and forearm skin. In particular, our previous studies showed that elasticity of face skin was significantly differed from forearm depending on full hydration. So, we have studied the surface properties of corneocyte using atomic force microscopy (AFM), assuming that the differences between face and forearm skin would be associated with the surface properties of corneocyte. The surface roughness of corneocyte and villus-like projections (VPs) were measured. Furthermore, qualitative comparison among the surface of face, forearm, and lip corneocyte was performed. Corneocytes were collected by tape-stripping on both face and forearm of 8 volunteers, and the bottom surface of corneocytes were measured at 40 ㎛ × 40 ㎛ using AFM. Results showed that the lower surface roughness of face corneocytes was 388.34 ± 86.189 nm, and that of forearm corneocytes was 662.27 ± 224.257 nm, which confirmed that the lower surface of forearm corneocytes was more rough than that of face corneocytes (p < 0.001). Compared with the amount of VPs, lip corneocytes were the highest followed by face corneocytes, and forearm corneocytes were the lowest. From these results, it is conclued that the surface properties of corneocytes are somewhat involved in the property differences between the face and the forearm skin and VPs can be a useful parameter for the study of corneocyte by site. In addition, AFM is a very useful device for the comparative study of nano-structural differences on the surface of corneocytes. More studies can lead to develop a new evaluation method of corneocytes.

• Journal of Veterinary Clinics
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• v.34 no.3
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• pp.225-227
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• 2017

This study was investigated the zoonotic possibility of Staphylococcus schleiferi by adherence to both canine and human corneocytes. Staphylococcus pseudintermedius, Staphylococcus aureus, and S. schleiferi were prepared and canine and human corneocytes were collected via double-sided tape. Adhesion to human corneocytes was higher for S. schleiferi than S. pseudintermedius but highest for S. aureus. Regarding the canine corneocytes, S. schleiferi was the least adhesive strain. Furthermore, S. pseudintermedius adhered to the entire surface of both human and canine corneocytes. S. aureus and S. schleiferi, however, adhered to the corneocyte peripheries. Thus, S. schleiferi may have zoonotic potential and the potential is higher than S. pseudintermedius.

• Journal of Physiology & Pathology in Korean Medicine
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• v.18 no.3
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• pp.729-733
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• 2004

Jaeum-Dan was reported new anti-aging effect formula previously. We investigated the physiological effects on human skin with Jaeum-Dan essence (JED). We made a skin application product as essence form contained oriental herbs, and measured various physiological effects such as skin moisture contents, red blood cell flow rates, skin surface temperature, corneocyte exfoliation and skin intensity when volunteers(female, n=40) used the application product for 4 weeks. The results were as follows: The skin moisture contents were not only increased in human face skin through using the product, but also red blood cell flow :rates in face skin were increased. After using the essence, the face skin temperature rose and corneocyte exfoliation was decreased. Furthermore the upper dermis intensity of skin in female face was subsequently increased 4 weeks later. The results indicate that the application product containing JED was improved the physiological effects of human facial skin..

• Journal of the Society of Cosmetic Scientists of Korea
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• v.44 no.1
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• pp.81-87
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• 2018

The objective of this article is to provide analytical tools for the scattering of stratum corneum (SC) and to check whether the optical clearing agents (OCAs) could be applied in optics affecting the scattering reduction. Dark field images of tape striped corneocyte separates scattered light of the SC from others in vitro. Several optical clearing agents were tested to reduce the scattering. Physical properties of SC such as water contents, keratin configuration and volume after OCAs treatment were investigated by FT-IR and 3D laser microscope. Several reducing sugars, monomeric sugars, sugar alcohol, and hyaluronic acid, which were used as humectants in cosmetic field, also reduced scattering. However, unlike dehydration in optics, water penetrated into the keratin in SC and scattering was decreased at low concentration of OCAs. In that condition, the volume of corneocyte was increased and stiffness seemed to decrease. The analyzing of tape-stripped SC, showed the change of optical and physical properties of corneocyte by optical clearing agents. The hydration of SC layer by optical clearing agents decreased the scattering of corneocyte and thus improved the skin appearance and moisturizing effect, which are important benefits in the cosmetic field and could provide new possibility to develop skin care study targeting at SC.

• The Korea Journal of Herbology
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• v.21 no.1
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• pp.25-31
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• 2006

Objectives : We investigated the aniti-aging effects of the cosmetic products with Pinus densiflora on human skin by using non-invasive instruments. Methods : We made cosmetic products containing the extract of the needles of red pine, and measured physiological effects such as skin wrinkles, clarity, evenness, lifting, corneocyte exfoliation after volunteers applied the cosmetic products for 8 weeks. Results : We observed a skin lifting effect and a decrease in corneocytes amount after using the cosmetic products for 4 weeks. And the wrinkles, evenness and clarity of face skin improved significantly. Conclusion : We concluded that the cosmetic products containing the extract of the needles of reel pine had an anti-aging effect on the human face skin.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.36 no.3
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• pp.193-198
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• 2010

Melanin is synthesized by the melanocytes in basal layer of epidermis and distributed over all the layers of skin including corneocytes in stratum corneum, outermost layer of the epidermis. Melanin of corneocytes was stained using Fontana-Masson method and quantified by image analysis. The correlation between melanin contents and skin color value was estimated in the skin of 71 Korean women. Melanin covering area (MCA) showed good correlation with $L^*$ value and $ITA^{\circ}$ (Individual Typology Angle) (r = 0.6049, 0.6651, respectively). MCA can be used as new parameter for skin color study and has potential application for evaluating the efficacy of the skin whitening cosmetics.

• Proceedings of the SCSK Conference
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• 2003.09a
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• pp.98-141
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• 2003

Lip chapping is a serious cosmetics problem, though remedies other than moisturizing have not been proposed. We investigated changes in the surface configurations of lip corneocytes and increased CD activity and improved chapping severity. Our results suggest that lip chapping can be activities of desquamation-regulating proteinases associated with lip chapping. Using scanning electron microscopy, villus-like projections were observed on the inner surfaces of most corneocytes from normal lips, whereas those with flatter surface were predominant in chapped lips. Further, cell surface area increased with the severity of lip chapping. Cathepsin D (CD)-like and chymotrypsin-like proteinase, which are also present in skin as desquamation-regulating proteinases, were detected in lip corneocytes, though only CD activity was found to decrease in severely chapped lips. Hydration was also lower in areas of lip chapping. Sequential topical application of apricot extract essence characterized as similar to senile xerosis rather than dry skin such as winter xerosis, as it shows a delayed transition of corneocytes through the stratum corneum, and the reduced CD activity may be one of the mechanisms that is further decreased by low hydration. We propose that an enhancement of both CD activity and lip moisture may be effective to improve lip chapping.

• YAKHAK HOEJI
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• v.40 no.2
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• pp.155-162
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• 1996

Hyperkeratinization is a dermatologic disorder, which is due to the increase of corneocyte cohesion force. Glycolic acid, an alpha hydroxy acid(AHA), has been used to breakdown the hyperkeratinization processes. However, it has a problem of skin irritation when applied topically, due to the strong acidity especially in high concentration. A molecular optimization of glycolic acid has been tried to reduce the skin irritation by the way of prodrug formation. Ethyl glycolate was synthesized by the esterification of glycolic acid with ethanol in acidic conditions in the presence of sulfuric acid, and examined under the spectroscopic trials, such as UV, IR, $^1H$-NMR, and GC-MS. The physicochemical and biopharmaceutical properties of the prodrug were also evaluated. Through the toxicological tests of both skin irritation and eye mucous irritation, it has been proved that ethyl glycolate was less irritant than glycolic acid, since the pH value of synthetic prodrug was higher than that of glycolic acid. In the penetration test through nude mouse skin by diffusion cell, ethyl glycolate was continuously hydrolyzed to glycolic acid, which was assayed form the receptor compartment. It was obtained that the penetrated amount of ethyl glycolate was five times higher than that of glycolic acid. These results suggest that ethyl glycolate might be a successful prodrug of glycolic acid to reduce the skin irritation and to increase the skin penetration as well.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.33 no.2
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• pp.99-104
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• 2007

The skin barrier function is one of the vital functions. Horny layer and epidermis among various skin layers play an important role in protecting body from environmental insults. Stratum corneum is completely formed by differentiation processes from the basal layer and newly formed corneocytes push the older ones from skin surface. In this study we investigated changes of various parameters - capacitance, transepidermal water loss (TEWL), pH and amount of removed corneocytes - according to the stratum corneum's depth. We removed the stratum corneum using tape stripping technique. We found that uppermost stratum corneum play an important role in skin surface moisturization and lower stratum corneum in protecting transepidermal water loss. In addition, skin pH is more acidic than surface pH. In the stratum corneum, skin pH is mote acidic in the deeper layer. Furthermore amount of removed corneocyte is also decreased according to depth because of stronger cell-cell union strength. From these results we suggest that stratum corneum has different characteristics depending on their position.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.30 no.3 s.47
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• pp.345-352
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• 2004

There are two paradigms to explain the atopic dermatitis. The first is outside-inside paradigm and the second is inside-outside paradigm. According to the outside-inside paradigm the best way to treat the atopic dermatitis is recovery of skin barrier function. The barrier function is maintained by the specific structure of stratum corneum, which is constructed from corneocytes and intercellular lipids. In terms of lipid structures of SC in atopic dermatitis and lamellar ichthyosis, they contain more fluid hexagonal gel structures in SC and show deficiencies in free fatty acids, especially long chains and certain ceramides. With this reason, moisturizer which has the lamellar structure and restoring function of intrinsic intercellualr long periodicity phase can maintain and restore the lamellar structure of intercellular lipids in SC. The moisturizers containing ceramide or pseudoceramide also seem to be reasonable therapy for atopic dermatitis and several skin diseases, which interrelated with impaired skin harrier. By the way, according to the inside-outside paradigm, immune response including helper T cells, IgE, eosinophils is related. It is effective treatment of atopic dermititis to restore imbalance between Th1 and Th2 cells. Even though several kinds of immune-suppressor were introduced, these can affect the intrinsic immune function. SPC and S1P, metabolites of ceramide, would be interesting because they have the function of wound healing and immune modulating properties.