• Microbiology and Biotechnology Letters
• /
• v.49 no.3
• /
• pp.413-424
• /
• 2021

In order to enhance rhizoremediation performance, which remediates contaminated soils using the interactions between plants and microorganisms in rhizosphere, it is required to develop effective microbial resources that simultaneously degrade contaminants and promote plant growth. In this study, heavy metal-resistant rhizobacteria, which had been cultivated in soils contaminated with heavy metals (copper, cadmium, and lead) and diesel were isolated from rhizospheres of maize and tall fescue. After that, the isolates were qualitatively evaluated for plant growth promoting (PGP) activities, heavy metal tolerance, and diesel degradability. As a result, six strains with heavy metal tolerance, PGP activities, and diesel degradability were isolated. Strains CuM5 and CdM2 were isolated from the rhizosphere soils of maize, and were identified as belonging to the genus Cupriavidus. From the rhizosphere soils of tall fescue, strains CuT6, CdT2, CdT5, and PbT3 were isolated and were identified as Fulvimonas soli, Cupriavidus sp., Novosphingobium sp., and Bacillus sp., respectively. Cupriavidus sp. CuM5 and CdM2 showed a low heavy metal tolerance and diesel degradability, but exhibited an excellent PGP ability. Among the six isolates, Cupriavidus sp. CdT2 and Bacillus sp. PbT3 showed the best diesel degradability. Additionally, Bacillus sp. PbT3 also exhibited excellent heavy metal tolerance and PGP abilities. These results indicate that the isolates can be used as promising microbial resources to promote plant growth and restore soils with contaminated heavy metals and diesel.

• Korean journal of food and cookery science
• /
• v.9 no.3
• /
• pp.195-197
• /
• 1993

This study investigated the contamination of trace metals on edible visceras : tongue, intestine, lung, testis, gira, blood, liver, stomach, and kidney. The edible visceras were selected at random from ten markets in Seoul. The edible visceras underwent freeze drying prior to analysis. The contents of arsenic, cadmium, cobalt, chromium, copper, magnases, molybdenum, lead, and zinc were detected by ICP(Inductively Coupled Plasma Spectrophotometry). The results showed that the levels of trace metals in all the samples fall within the tolerance limit and cadmium in lung tended to be high(>0.1 ppm). Therefore, we may study and investigate continuously on the food contamination of heavy metals for the public health.

• Journal of Korean Society for Quality Management
• /
• v.25 no.1
• /
• pp.135-141
• /
• 1997

The Copper-Clad Laminate (CCL) is a main electronic component of specialtype printed circuit boards (PCBs) such as Silver Through Hole PCB. This CCL should have high reliability under the aging test, and usually the test is done at a higher temperature (110-$150^{\circ}C$) than the normal. Then, this test condition of high temperature may cause such quality problems as hole eccentricity and reduction of distance between part holes. After measuring the CCL shrinkage affected by temperature, the correction factor of a press mold was a, pp.ied to solve these problems. The results showed that the tolerance of hole pitch(${\pm}$$100{\mu}m$) was satisfactory and the internal and external failure costs were reduced by 55%.

• Journal of the Korean Society for Marine Environment & Energy
• /
• v.9 no.3
• /
• pp.160-167
• /
• 2006

Harpacticoid copepod Tigriopus japonicus is widely distributed in coastal waters of Korea and plays important role in marine trophic structure as a first consumer. In this study, a series of experiment were conducted to test the potential of the species as a standard test species for marine bioassay. Tolerance on salinity and pH, sensitivity on the reference materials(copper sulfate and cadmium chloride) and response on the ocean dumping materials(waste sludge) we re tested to identify if the species satisfy the basic criteria as standard species for marine bioassay. The nauplius of the species($100{\sim}200{\mu}m$) showed wide tolerance on salinity with >90.0% survival rates exposed to $5.0{\sim}35.0psu$ for 48 h. Wide adaptability on pH's were also observed from 6.3 to 8.2 with >90.0% survival rates during the test. $LC_{50}$ values for copper sulfate and cadmium chloride were $3.6{\pm}0.7ppm,\;1.7{\pm}0.8ppm$, respectively. The variations in mortality between replicates were less than 10.0%. Comparison of $LC_{50}$ values indicated that T. japonicus nauplius was lower sensitive to copper sulfate than the most marine crustaceans included copepods, however, the sensitivity of test animal to cadmium chloride higher than the adults of copepod T. japonicus, Paracalanus parvus, and marine rotifer Brachinonus plicatilis. There were significant concentration-response relationship in the mortality of T. japonicus nauplius using the elutriates of three ocean dumping materials(industrial waste sludge). 48 h $LC_{50}$ values we re $31.1{\pm}1.1%$ for the elutriate of sludge from leather processing company and $54.4{\pm}15.1%$ for that of dye production company. Based on the above experimental results, bioassay using benthic harpacticoid T. japonicus nauplius must be a good estimation tool for marine ecotoxicological assessment of waste or chemicals. Wide tolerance on the salinity and pH, and significant linear relationship between concentration and response(mortality) supported the high potential of the species as a standard test species.

• The Bulletin of The Korean Astronomical Society
• /
• v.36 no.2
• /
• pp.153.2-153.2
• /
• 2011

The IGRINS (Immersion GRating INfrared Spectrometer) is a high resolution wide-band infrared spectrograph developed by Korea Astronomy and Space Science Institute (KASI) and the University of Texas at Austin (UT). Immersion grating is a key component of IGRINS, which disperses the input ray by using a Silicon material with a lithography technology. Opto-mechanical mount for the immersion grating is important to keep the high spectral resolution and the optical alignment in a cold temperature of $130{\pm}0.06K$. The optical performance of immersion grating can maintain within the de-center tolerance of ${\pm}0.05mm$ and the tip-tilt tolerance of ${\pm}1.5arcmin$. The mount mechanism utilizes the flexure and the kinematic support design to satisfy the requirement and the operation condition. When the IGRINS system is cooled down to a cold temperature, three flexures compensate the thermal contraction stress due to the different material between the immersion grating and the mounting part(Aluminum 6061). They also support the immersion grating by an appropriate preload. Thermal stability is controlled by a copper strap with proper dimensions and a heater. Generally structural and thermal analysis was performed to confirm the mount mechanism. This talk presents the opto-mechanical mount design of the immersion grating of IGRINS.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
• /
• 2008.11a
• /
• pp.421-421
• /
• 2008

Generally, the polymer thick-film resistors for embedded organic or hybrid substrate are patterned by screen printing so that the accuracy of resistor pattern is not good and the tolerance of resistance is too high(${\pm}$20~30%). To reform these demerits, a method using Fodel$^{(R)}$ technology, which is the patterning method using a photosensitive resin to be developable by aqueous alkali-solution as a base polymer for thick-film pastes, was recently incorporated for the patterning of thermosetting thick-film resistor paste. Alkali-solution developable photosensitive resin system has a merit that the precise patterns can be obtained by UV exposure and aqueous development, so the essential point is to get the composition similar to PSR(photo solder resist) used for PCB process. In present research, we made the photopatternable resistor pastes using 8 kinds of epoxy acrylates and a conductive carbonblack (CDX-7055 Ultra), evaluated their developing performance, and then measured the resistance after final curing. To become developable by alkali-solution, epoxy acrylate oligomers with carboxyl group were prepared. Test coupons were fabricated by patterning copper foil on FR-4 CCL board, plating Ni/Au on the patterned copper electrode, applying the resistor paste on the board, exposing the applied paste to UV through Cr mask with resistor patterns, developing the exposed paste with aqueous alkali-solution (1wt% $Na_2CO_3$), drying the patterned paste at $80^{\circ}C$ oven, and then curing it at $200^{\circ}C$ during 1 hour. As a result, some test compositions couldn't be developed according to the kind of oligomer and, in the developed compositions, the measured resistance showed different results depending on the paste compositions though they had the same amount of carbonblack.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.14 no.6
• /
• pp.3114-3119
• /
• 2013

The mangrove ecosystems have the capacity to act as a sink of heavy metals entering aquatic ecosystems. Despite their potential exposure to metal contaminated sediments, mangroves appear to be highly tolerant to heavy metals. In this study, we cloned metal tolerance gene from mangrove plant. Using CTAB method, RNA were isolated from leaves and root tissue of Rhizophora stylosa habitated at Weno island in Micronesia Chuuk lagoon using CTAB method and phytochelatin synthase 1 (PCS1) gene was cloned using gene specific primers. Expression of PCS1 gene was increased 1.91 fold and 2.72 fold in mangrove propagules exposed to 100 ppb Cd and 10 ppb Cu, respectively. These results indicate that expression of PCS1 gene are promising tools for health assessment of mangrove ecosystem.

• Journal of Plant Biotechnology
• /
• v.48 no.4
• /
• pp.246-254
• /
• 2021

Environmental stresses are estimated to have reduced global crop yields of wheat by 5.5%. However, traditional approaches for the transfer of resistance to these stresses in wheat plants have yielded limited results. In this regard, genetic transformation has undoubtedly opened up new avenues to overcome crop losses due to various abiotic stresses. Particle bombardment has been successfully employed for obtaining transgenic wheat. However, most of these procedures employ immature embryos, which are not available throughout the year. Therefore, the present investigation utilized mature seeds as the starting material and used the calli raised from three Moroccan durum wheat varieties as the target tissue for genetic transformation by the biolistic approach. The pANIC-5E plasmid containing the SINA gene for drought and salinity tolerance was used for genetic transformation. To enhance the regeneration capacity and transformation efficiency of the tested genotypes, the study compared the effect of copper supplementation in the induction medium (up to 5 μM) with the standard MS medium. The results show that the genotypes displayed different sensitivities to CuSO₄, indicating that the transformation efficiency was highly genotype-dependent. The integration of transgenes in the T₀ transformants was demonstrated by polymerase chain reaction (PCR) analysis of the obtained resistant plantlets with primers specific to the SINA gene. Among the three genotypes studied, 'Isly' showed the highest efficiency of 9.75%, followed by 'Amria' with 1.25% and 'Chaoui' with 1%.

• Journal of Korean Society of Forest Science
• /
• v.85 no.2
• /
• pp.329-339
• /
• 1996

This study is to investigate the effect of mycorrhizal development, growth, nutrient absorption of P. thunbergii seedlings inoculated with Pisolithus tinctorius(Pt. KJ-1) in relation to toxic materials in soil. The concentrations on copper solution applied to the soils were 0, 40, 120, 260, 430ppm. The results are summarized as follows : 1. The germination of P. thunbergii with Pt. increased greater than without ectomycorrhizal fungi. Mycorrhizal development showed a significant decreasing trend at high concentration, and tolerant Cu test with Pt. in agar plate media showed a decreasing trend at a high level. 2. P. thunbergii seedlings inoculated with Pt. showed that the shoot length was significantly promoted at 40, 120ppm copper levels, and that of noninoculated seedlings had the lowest effect in 430ppm copper level. 3. The outer shape of ectomycorrhizal root tips after inoculating Pt. on P. thunbergii seedlings appeared as a monopodial type, a fern-like type, and a cluster-like type at 0ppm, 40ppm levels, but only monopodial type came out at 260ppm, 430ppm copper levels. 4. Root length, no. of juvenile leaves, total length of juvenile leaves, total dry weight, no. of needles and total length of needles of P. thunbergii with Pt. increased greater than those of noninoculated seedling. Growth response of P. thunbergii seedling inoculated with Pt. increased significantly at 40ppm, 120ppm Cu levels. 5. As a result of analysis of growth medium, pH, Na, CEC increased at higher Cu level, and total nitrogen, organic matters, available phosphorous, K, Ca and Mg decreased at 40ppm, 120ppm Cu levels. As a result of an analysis about a copper within soils, it appeared having high Cu-concentration at 0ppm level of an inoculated Pt. and high Cu-concentration in noninoculated Pt. than in inoculated Pt. at higher level. 6. As a result of an analysis of shoot, N, P, and K-concentration were higher in noninoculated seedlings than in inoculated seedlings, and Cu-concentration was higher in inoculated seedlings than in noninoculated seedlings. The analysis of root resulted in a high N-concentration at 40ppm, 120ppm levels, in a high P-concentration in inoculated seedlings and in a high Cu-concentration in noninoculated seedlings. Cu-concentration was significantly higher at root than at shoot.

• Journal of environmental and Sanitary engineering
• /
• v.24 no.3
• /
• pp.28-36
• /
• 2009

We have closely examined the concentration change characteristics, emission amounts, and the material balance of hazardous air pollutants at both early and later stages of the prevention facilities. These results will be uses as the basic data when preparing for the regulatory and management plans for hazardous air pollutants. The results of the study on heavy metals illustrated that the content of heavy metals in sludge across five facilities were as follows: copper> zinc> chrome> nickel> cadmium> mercury. In terms of heavy metal content in swage sludge, the sludge in incinerating facilities other than the sludge in the D incinerating facility containing industrial water waste, was examined in order to satisfy the ocean contamination standard and fertilizer specifications. Most of the items were shown to have satisfied the emission tolerance standards in the latter part of the prevention facilities(The average elimination rate was over 90%). Therefore, it is concluded that swage sludge containing high-concentrate heavy metals needs to be incinerated rather than recycled as fertilizer.