• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.110-110
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• 2017

Stomata are the main gateways for water and air transport between leaves and the environment. Inward-rectifying potassium channels regulate photo-induced stomatal opening. Rice contains three inward rectifying shaker-like potassium channel proteins, OsKAT1, OsKAT2 and OsKAT3. Among these, only OsKAT2 is specifically expressed in guard cells. Here, we investigated the functions of OsKAT2 in stomatal regulation using three dominant negative mutant proteins, OsKAT2(T235R), OsKAT2(T285A) and OsKAT2(T285D), which are altered in amino acids in the channel pore and at a phosphorylation site. Yeast complementation and patch clamp assays showed that all three mutant proteins lost channel activity. However, among plants overexpressing these mutant proteins, only plants overexpressing OsKAT2(T235R) showed significantly less water loss than the control. Moreover, overexpression of this mutant protein led to delayed photo-induced stomatal opening and increased drought tolerance. Our results indicate that OsKAT2 is an inward-rectifying shaker-like potassium channel that mainly functions in stomatal opening. Interestingly, overexpression of OsKAT2(T235R) did not cause serious defects in growth or yield in rice, suggesting that OsKAT2 is a potential target for engineering plants with improved drought tolerance without yield penalty.

• Journal of the Korean Society of Safety
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• v.16 no.4
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• pp.200-207
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• 2001

The transmission of harmonic vibratory power form a vibrating rigid body into a supporting plate through passive and active isolators is investigated theoretically and experimentally. The theoretical model allows for the transmission of vertical and horizontal harmonic forces and moments about all three coordinate sun. The experiment is to use vibration actuators attached to the intermediate mass of the two-stage mount to minimize the rotational and translational vibration of the intermediate mass. The performance is done by measuring the vibration at the error sensors due to the primary vibration source and measuring the transfer functions from the control sources to the error sensors. Results show that over a frequence range from 1 to 100Hz, transmission into the supporting plate can be reduced substantially by employing in parallel with existing passive isolators, active isolators adjusted to provide appropriate control force amplitudes.

• Proceedings of the KSRS Conference
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• 2003.11a
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• pp.225-227
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• 2003

Traditional photogrammetry and satellite image rectification technique have been developed based on control-points for many decades. These techniques are driven from linked points in image space and the corresponding points in the object space in rigorous colinearity or coplanarity conditions. Recently, digital imagery facilitates the opportunity to use features as well as points for images rectification. These implementations were mainly based on rigorous models that incorporated geometric constraints into the bundle adjustment and could not be applied to the new high-resolution satellite imagery (HRSI) due to the absence of sensor calibration and satellite orbit information. This research is an attempt to establish a new Line Based Transformation Model (LBTM), which is based on linear features only or linear features with a number of ground control points instead of the traditional models that only use Ground Control Points (GCPs) for satellite imagery rectification. The new model does not require any further information about the sensor model or satellite ephemeris data. Synthetic as well as real data have been demonestrated to check the validity and fidelity of the new approach and the results showed that the LBTM can be used efficiently for rectifying HRSI.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 1996.10a
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• pp.324-329
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• 1996

Vibration characteristics of the feeding system in a CD-ROM drive are identified by a theoretical modeling as well as vibration experiments. For this purpose, we establish a vibration model due to the rigid-body motion and perform the modal testings using the impact hammer and shaker. The analysis and experiments show that the feeding system has three rigid-body vibration modes in the low-frequency region and two of them come from the tilting modes. In order to remove the harmful tilting modes for the tracking servo control, a methodology to find the optimal positions of the dampers is also proposed in this study.

• 제어로봇시스템학회:학술대회논문집
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• 2001.10a
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• pp.61.4-61
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• 2001

This research is the development of a distributed flexible tactile sensor for service robots using PVDF (polyvinylidene fluoride) film for the detection of the contact state in real time. The tactile sensor which has 8$\times$8 taxels is fabricated using PVDF film and flexible circuitry. The proposed fabrication method is simple and easy to make the sensor in the laboratory without using any special equipment. Experimental results on static and dynamic properties are obtained. In order to investigate the properties of the sensor, the sensor output to the arbitrary forces and frequencies are measured using the shaker with the force sensor.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2006.11a
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• pp.515-518
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• 2006

A vibration test for a cylindrical rod inserted on the grid support structure was tested using the sine sweep excitation method with closed loop force control. The effect of the mounting location of a test rod on the vibration characteristics of a rod continuously supported by the full size($16{\times}16$) grid support was identified. An electromagnetic vibration shaker, non-contact displacement sensor and HP/VXI data acquisition device were used and TDAS software was also used as a data sampling and processing tools. The natural frequencies and mode shape of the test rod were consistent with the previous works of a rod vibration test with partial grids($3{\times}3,\;5{\times}5\;and\;7{\times}7$). The frequency characteristics of the rod according to the mounting location were shown clear discrepancies, but mode shapes were nearly same. As the test rod closes to the bottom clamping region of the spacer grid, peak vibration amplitudes of the rod become smaller.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.26 no.11
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• pp.831-835
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• 2013

In this study, we fabricated a micro $Pb(Zr,Ti)O_3$ (PZT) film piezoelectric cantilever with a Si proof mass and dual beams through MEMS process. The size of the beam and the integrated Si proof mass were about $4,320{\mu}m{\times}290{\mu}m{\times}12{\mu}m$ and $1,380{\mu}m{\times}880{\mu}m{\times}450{\mu}m$ each. To reduce the air damping and have the larger displacement of dual beams was used for design. After mounting micro PZT film piezoelectric cantilever on shaker, we measured the resonance frequency and a output voltage while making resonant frequency changed. The resonant frequency and the highest average power of the cantilever device were 110.2 Hz and 0.36 ${\mu}W$ each, at 0.8 g acceleration and 23.7 $k{\Omega}$ load resistance, respectively.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.120.1-120
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• 2003

White rot on Allium species recently had a high incidence as increased cultivating areas of tropical garlic types in Korea. Two types of Sclerotium have been known as causal agents producing different size and shapes of sclerotia in infested fields. We developed a new method for isolation of two types of sclerotia from infested field soils that can be used for ecological study of sclerotium spp. and establishment of control strategy. Soil samples collected from heavily infested fields were evenly mixed and placed on a automatic sieve shaker connected with tap water. After 10 min. of shaking, residues on 0.5mm and 0.25mm sieve were separately collected and suspended with 70％ sugar solution, which method floats sclerotia in aqueous layer. Then, floated fraction was carefully separated and mixed with a same volume of 1％ sodium hypochlorite solution to differentiate with organic materials. This method provides direct count of sclerotia under dissecting microscopy.

• Microbiology and Biotechnology Letters
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• v.6 no.2
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• pp.75-80
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• 1978

This study was conducted to investigate the condition of enhancing glutathione content of yeast. Rhodotorula glutinis in various kind of yeasts produced high content of glutathione and dry cell by cultivating for 48 hours at 30'C and pH 6.0 on reciprocal shaker. In order to enhance the glutathione content, as 0.7％ of amino acid was applied into the medium. Glutathione was produced high for 36 hours cultivation. When glutamic acid, cysteine and glycine composing the glutathione were added, glutathione content increased to 219 $\mu\textrm{g}$/ml. However the control showed to 73 $\mu\textrm{g}$/ml.

• Journal of Microbiology and Biotechnology
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• v.28 no.9
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• pp.1511-1516
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• 2018

In this study, we investigated Indole-3-acetic acid (IAA) production by a rice phylloplane bacteria, Enterobacter sp. DMKU-RP206, using sweet whey as a feed stock instead of lactose. We succeeded in using sweet whey for Enterobacter sp. DMKU-RP206 to produce 3,963.0 mg IAA/l with the optimal medium containing 1.48% sweet whey, 1.42% yeast extract and 0.88% $\text\tiny{L}$-tryptophan. The medium pH was adjusted to 6 and the culture conditions were shaking at 200 rpm on an orbital shaker at $30^{\circ}C$ for 3 days. We also evaluated the effect of IAA in culture filtrates of Enterobacter sp. DMKU-RP206 on the promotion of jasmine rice growth in a pot experiment. Compared with the negative control (without IAA), the result showed that biosynthetic IAA produced by Enterobacter sp. DMKU-RP206 significantly increased the growth of jasmine rice (Oryza sativa L. cv. KDML105) in terms of length and dry weight of shoot. This work thus reveals the impact of IAA produced by Enterobacter sp. on the promotion of jasmine rice growth.