• Biomolecules & Therapeutics
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• v.30 no.2
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• pp.145-150
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• 2022

In this study, we investigated the influence of galangin on vascular contractibility and to determine the mechanism underlying the relaxation. Isometric contractions of denuded aortic muscles were recorded and combined with western blot analysis which was performed to measure the phosphorylation of phosphorylation-dependent inhibitory protein of myosin phosphatase (CPI-17) and myosin phosphatase targeting subunit 1 (MYPT1) and to evaluate the effect of galangin on the RhoA/ROCK/CPI-17 pathway. Galangin significantly inhibited phorbol ester-, fluoride- and thromboxane mimetic-induced vasoconstrictions regardless of endothelial nitric oxide synthesis, suggesting its direct effect on vascular smooth muscle. Galangin significantly inhibited the fluoride-dependent increase in pMYPT1 and pCPI-17 levels and phorbol 12,13-dibutyrate-dependent increase in pERK1/2 level, suggesting repression of ROCK and MEK activity and subsequent phosphorylation of MYPT1, CPI-17 and ERK1/2. Taken together, these results suggest that galangin-induced relaxation involves myosin phosphatase reactivation and calcium desensitization, which appears to be mediated by CPI-17 dephosphorylation via not PKC but ROCK inactivation.

• Biomolecules & Therapeutics
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• v.32 no.3
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• pp.361-367
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• 2024

In this study, we investigated the efficacy of kaempferol (a flavonoid found in plants and plant-derived foods such as kale, beans, tea, spinach and broccoli) on vascular contractibility and aimed to clarify the detailed mechanism underlying the relaxation. Isometric contractions of divested muscles were stored and linked with western blot analysis which was carried out to estimate the phosphorylation of myosin phosphatase targeting subunit 1 (MYPT1) and phosphorylation-dependent inhibitory protein for myosin phosphatase (CPI-17) and to estimate the effect of kaempferol on the RhoA/ROCK/CPI-17 pathway. Kaempferol conspicuously impeded phorbol ester-, fluoride- and a thromboxane mimetic-derived contractions regardless of endothelial nitric oxide synthesis, indicating its direct effect on smooth muscles. It also conspicuously impeded the fluoride-derived elevation in phospho-MYPT1 rather than phospho-CPI-17 levels and phorbol 12,13-dibutyrate-derived increase in phospho-CPI-17 and phospho-ERK1/2 levels, suggesting the depression of PKC and MEK activities and subsequent phosphorylation of CPI-17 and ERK1/2. Taken together, these outcomes suggest that kaempferol-derived relaxation incorporates myosin phosphatase retrieval and calcium desensitization, which appear to be modulated by CPI-17 dephosphorylation mainly through PKC inactivation.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.16 no.4
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• pp.99-114
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• 2013

In this study, constructability of ecologocal stream restoration process was evaluated and improved approaches for habitat were suggested. The study site is Gucheon River in Geoje city where conducted ecologocal restoration work within maintaining its flood control function. Application of ecological design factors and constructability in the process from planning to construction was analyzed and its results are as follows: In the process of planning and designing, it was focused more on the naturality of Gucheon River rather than the human convinience and ecological design factors were applied within the ecological capacity of the site. First, the indexes for constructability evaluation is selected. It was classified into three major categories as construction quality, design quality and construction administration system. Each index has details, so there are twenty sub indexes for contractibility evaluation. Second, the evaluation results shows that the index most in need of improvement was plumbing construction, followed by Stone construction and pavement construction. design concept was evaluated as appropriate in entire categories. Finally it is suggested that it can be improved in both process of design to enhance the technology and process of construction to enhance the quality management. The constructability of ecological stream needs adaptive management and it must be discussed with its designing which is at the stage before construction. Also it needs discussion with its designer constantly and feed-back process.

• Journal of Conservation Science
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• v.26 no.2
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• pp.183-190
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• 2010

We fabricated urethane material based on properties of reversible and unfading for antic-ceramics restoration. This material with low viscosity was made hardness control possible that user want. And it have very strong adhesion and shear strength properties and is shown the best properties for pigment filling, anti-contractibility, coloring as like epoxy system materials. Particularly, the yellowing and ir-reversibility problem in epoxy restoration material were finally solved. So, there is guarantee in the eternity and stabilization of restoration for antic-celamics. And in order to show the reversible state of the restoration, we successfully dissolve this urethane materials in solvent after perfect restoring subsequently.

• Journal of Conservation Science
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• v.25 no.3
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• pp.293-298
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• 2009

We fabricated the gap-filling material for antic-ceramics restoration which were made up polyvinyl butyral (butvar) base and silane system additive in iso-propyl solvent. This was made hardness control possible that user want. And it have very strong adhesion and shear strength properties and is shown the best properties for pigment filling, anti-contractibility, coloring as like epoxy system materials. Particularly, the yellowing and ir-reversibility problem in Epoxy restoration material were finally steadiness. So, there is guarantee in the eternity and stabilization of restoration for antic-celamics. And in order to show the reversible state of the restoration, we successfully dissolve this polyvinyl butyral gap-filling materials in solvent after perfect restoring the Punchong plate from sam-chun temple site subsequently.

• Proceedings of the Korea Institute of Fire Science and Engineering Conference
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• 2010.04a
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• pp.371-376
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• 2010

The strength of steel material in a concrete filled steel tube (CFT) is reduced in fire, but the filled interior concrete structurally ensures the fire resistance due to its high thermal capacity. More, the contractibility of CFT is excellent since it can be constructed without form work. This research analyzed the interior concrete strength and deformation characteristics, which are the influence factors of the fire resistance of CFT, in proportion to the axial load ratio. The fire resistance performance according to changes of the axial load ratio showed great fluctuation. As $280{\times}280{\times}6$ CFT columns with the concrete strengths of 24 MPa and 40 MPa and the axial load ratios of 0.9, 0.6, and 0.2 in accordance with KS F 2257-1 and 7 were heated with loading to examine the fire resistance performance, the 24 MPa concrete exhibited the fire resistance time as 27, 113, and 180 minutes for the axial load ratios, 0.9, 0.6, and 0.2 respectively. In case of 40 MPa concrete, the fire resistance time were turned out to be 19 and 28 minutes for the axial load ratios, 0.9 and 0.6 respectively. The results of 40 MPa concrete showed the much lower fire resistance performance when comparing with those of 24 MPa concrete. The fire resistance performance was not increased significantly when the axial load ratio was reduced. Therefore, the deceased fire resistance performance of high strength concrete is assumed to be caused by the internal pressure increase upon the heat application.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.41 no.2
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• pp.89-93
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• 2008

Goldfish were exposed to radioactive isotope $^{18}F$, which is used for positron emission tomography (PET). $^{18}F$ is created when the oxygen in water combines with hydrogen on exposure to positrons accelerated by a cyclotron. The temporal change in intestine contractility was measured by physiograph after the exposure to $^{18}F$. The distance between the goldfish and 580 mCi of $^{18}F$ was approximately 4 cm and the exposure was for 4 hrs. The absorption level calculated from the distance, exposure time, and half-life of $^{18}F$ was approximately 2 Gy. The contractile activity of goldfish intestine was lowest on the first day and increased gradually to 100% of the control level by a period of between 5 and 36 days.

• Journal of the Korean Society of Clothing and Textiles
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• v.31 no.6 s.165
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• pp.974-984
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• 2007

The purpose of this study was to find differences in evaluation criteria and to find differences in preferred images based on benefits segmented groups of jeans products consumers. Male and female Korean university students participated in the study. Quota sampling method was used to collect the data based on gender and a residential area of the respondents. Data from 492 questionnaires were used in the analysis. Factor analysis, Cronbach's alpha coefficient, cluster analysis, one-way ANOVA, and post-hoc test were conducted. As a result, respondents who seek multi-benefits considered aesthetic criteria(e.g., color, style, design, fit) and quality performance criteria(e.g., durability, ease of care, contractibility, flexibility) more importantly when evaluating and purchasing jeans products. Respondents who seek brand name considered extrinsic criteria(e.g., brand reputation, status symbol, country of origin, fashionability) more importantly than respondents who seek economic efciency. Respondents who seek multi-benefits such as attractiveness, fashion, individuality, and utility tend to prefer all the images: individual image, active image, sexual image, sophisticated image, and simple image when wearing jeans products. Respondents who seek fashion are likely to prefer individual image, and respondents who seek brand name more prefer both individual image and polished image. Mean while, respondents who seek economical efficiency less prefer sexual image and polished image.