• 한국미생물·생명공학회지
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• 제2권1호
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• pp.29-35
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• 1974

We have studied the applicability of the principles and inherent advantages of the two-stage dontinuous uclture technique to an enzyme process for the purpose of improving and optimizing the productivity of 3-ketosteroid-delta-1-dehydrogenase. By using a two-stage continuous culture system, the growth st ageand enzyme produdtion stage are separated. In each stage an optimal set of toperaing conditions was determined, and this was tested for feasibility for the period of 10 days. During this period, at least 70％ of the maximum enzyme productivity could be maintained. The important design parameters studied are: (1) optimal specific growth rate in the first stage which corresponds to the maximal cell productivity, (2) the optimal dilution rate in the second stage which in turn determines the size of second stage fermentor and the mean residence time of cells in the second stage, (3) cell concentration in both stages, add (4) the specific enzyme productivity and enzyme productivity of the second stage. In addition, by using two-stage continuous culture system we have been able to reduce or eliminate the effect of catabolite repression due to high medium concentration and the adverse effect of the solvent used to dissolve the inducer. We have found the balance between the opposing effects of induction and repression in the second stage judging from the observation that the enzyme productivity goes through a maximum.

• 대한전자공학회:학술대회논문집
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• 대한전자공학회 2000년도 하계종합학술대회 논문집(1)
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• pp.85-88
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• 2000

Distributed Control System(DCS) is one of the best solutions to implement control systems because it provides continuous observation of control process and execution of commands to induce proper operations. In this paper, a design of control network for DCS in nuclear power plant is proposed. The proposed control network on DCS has a simple architecture and deterministic property. Thus, the proposed control network offers hard real-time periodic service. It also has redundant media for the fault-tolerance. As a result, high safety and reliability required in nuclear power plant are guaranteed.

• Mycobiology
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• 제51권3호
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• pp.164-168
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• 2023

This study examined the effects of myristate on an asymbiotic culture of Rhizoglomus intraradices, a species of arbuscular mycorrhizal fungi (AMF; Glomeromycota). Mycelial growth and sporulation in a modified medium containing myristate were observed. The findings demonstrated that myristate induced R. intraradices spore formation, with daughter spores having a smaller diameter than the parent spores. This observation is consistent with previous studies on other Rhizoglomus species. Further studies are needed to investigate the potential for continuous culture, mass production using daughter spores, and the application of AMF colonization techniques in plants.

• 대한의용생체공학회:의공학회지
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• 제16권4호
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• pp.415-424
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• 1995

Combined models, specified by two or more modeling formalisms, can represent a wide variety of complex systems. This paper describes a methodology for the development of combined models in two model types of discrete event and continuous process. The methodology is based on transformation of continuous state space into discrete one to homomorphically represent dynamics of continuous processes in discrete events. This paper proposes a formal structure which can combine model of the DES and the CS within a framework. The structure employs the DEVS formalism for the DES models and differential or polynomial equations for the CS models. To employ the proposed structure to specify a DEVS/CS combined model, a modeler needs to take the following steps. First, a modeler should identify events in the CS and transform the states of the CS into the DES. Second, a modular employs the formalism to specify the system as the DES. Finally, a moduler developes sub-models for the CS and continguos states of the DES and establishs one-to-one correspondence between the sub-models and such states. The proposed formal structre has been applied to develop a DEVS/CS combined model for the human cardiovascular system. For this, the cardiac cycle is partitioned into a set of phases based on events identified through observation. For each phase, a CS model has been developed and associated with the phase. To validate the DEVS/CS combined model developed, then simulate the model in the DEVSIM + + environment, which is a model simulation results with the results obtained from the CS model simulation using SPICE. The comparison shows that the DEVS/CS combined model adequately represents dynamics of the human heart system at each phase of cardiac cycle.

• 대한약침학회지
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• 제14권3호
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• pp.19-45
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• 2011

Objectives: This study was performed to analyse the effects of Sweet Bee Venom(Sweet BV-pure melittin, the major component of honey bee venom) on the central nervous system in rats. Methods: All experiments were conducted at Biotoxtech Company, a non-clinical studies authorized institution, under the regulations of Good Laboratory Practice (GLP). Male rats of 5 weeks old were chosen for this study and after confirming condition of rats was stable, Sweet BV was administered in thigh muscle of rats. And checked the effects of Sweet BV on the central nervous system using the functional observational battery (FOB), which is a neuro-toxicity screening assay composed of 30 descriptive, scalar, binary, and continuous endpoints. And home cage observations, home cage removal and handling, open field activity, sensorimotor reflex test/physiological measurements were conducted. Results: 1. In the home cage observation, there was not observed any abnormal signs in rats. 2. In the observation of open field activity, the reduction of number of unit areas crossed and rearing count was observed caused by Sweet BV treatment. 3. In the observation of handling reactivity, there was not observed any abnormal signs in rats. 4. In the observation of sensorimotor reflex tests/physiological measurements, there was not observed any neurotoxic signs in rats. 5. In the measurement of rectal temperature, treatment of Sweet BV did not showed great influences in the body temperature of rats. Conclusions: Above findings suggest that Sweet BV is relatively safe treatment in the central nervous system. But in the using of over dose, Sweet BV may the cause of local pain and disturbance of movement. Further studies on the subject should be conducted to yield more concrete evidences.

• Journal of Gastric Cancer
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• 제24권2호
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• pp.199-209
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• 2024

Purpose: Long-term outcomes of patients with positive lateral margins (pLMs) after endoscopic submucosal dissection (ESD) for early gastric cancer (EGC). This study aimed to evaluate the remnant cancer and survival rates of patients with pLMs compared with those who underwent curative resection. Materials and Methods: A retrospective analysis was performed on consecutive patients with pLMs as the only non-curative factor of expanded indication who underwent ESD for EGC with a follow-up duration of 5 years or more. The rates of remnant cancer, recurrence, and survival were analyzed and compared to those of control patients who underwent curative resection by propensity score matching. Results: Among 3,515 patients treated with ESD between 2005 and 2018, 123 non-curative EGCs were retrospectively analyzed. A total of 108 patients were followed up without endoscopic or surgical resection for 8.2 years. The control group was matched in a 1:1 ratio with patients with EGC who underwent curative resection after ESD. The observation group with pLMs had a higher incidence of remnant cancer (25.9%; 28/108) compared to that in the curative resection group (0/108; P=0.000). The remaining tumors were treated with surgical or endoscopic resection, and no additional recurrences were observed. The overall survival analysis demonstrated no significant difference between the observation and curative resection groups (P=0.577). Conclusions: No difference was observed in the overall survival rate between observation and curative resection groups. Therefore, observation may be a possible option for incomplete ESD with pLMs if continuous follow-up is performed.

• 한국측량학회지
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• 제15권1호
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• pp.9-20
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• 1997

정밀 수준측량은 지각의 상하변동을 조사하거나 지진예지를 위하여 중요한 수단의 하나이다. 본 연구는 대기층에서 발생하는 빛의 굴절이 경사지 수준측량에서 어떠한 영향을 미치는가를 고찰하는데 목적이 있다 본 연구에서는 Kukkamaki의 이론과 기상관측 자료를 토대로 Kukkamaki의 보정량이 경사지 전후시 수준측량오차에 어떠한 영향을 미치는가를 실제 실험을 통하여 시도하여 보았다. 실제적으로 경사 1고정측점(2/100∼4/100)에서 전후시 반복관측과 경사지 연속 관측 루트를 통하여 빛의 굴절의 영향과 그 특성을 고찰하였다 특히, 경사지 수준측량(2/100-4/100)에서 빛의 굴절오차를 최소화 하기위여는 시준거리를 25-30 m로 하는 것이 이상적이다. 또한, 경사지연속관측 결과 Kukkamaki의 계산값과 실측값이 거의 일치하고 있음을 알 수 있었다.

• Asian-Australasian Journal of Animal Sciences
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• 제24권10호
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• pp.1365-1371
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• 2011

The intensity and duration of sexual behavior in Bos indicus was assessed through the continuous observation of sexual receptivity. Two groups of cows were formed: only synchronized (n = 50) and other group further superovulated (n = 20). An intravaginal implant that released progesterone over 9 d was used. After removing the implant, 25 mg of $PGF_{2{\alpha}}$ was administered. In the superovulated group, the administration of 280 mg (Follicle stimulant hormone) FSH-P1 per cow with a decreasing dosage over 4 d was utilized. In both groups, behavioral observations began at the moment of implant removal. Sexual behavior was analyzed using a Kruskal-Wallis test to compare the mean of hours in estrus, effective mountings and number of mounts/hour during estrus. A nonparametric survival analysis was performed using the time in two ways: i) when an event happened it was placed in a 24 h timeframe and, ii) the time of observation in continuous form (96 h) assessing the difference between curves by the log rank test Chi-square. The only significant difference was the number of mounts/h during receptivity (p<0.05). In the superovulated group three periods of sexual activity during the day were identified, with these events being of greater frequency and duration than the synchronized group (p = 0.02); besides, the superovulated group began estrus before the synchronized group (p = 0.0035) when using the total period. In a simulation study, when the number of observations went from two (06:00-18:00) to three periods (06:00, 12:00 and 18:00) cows detected accurately (<6 h after the onset) increased more than 20%. The results show that superovulated cows presented greater intensity and duration of sexual activity in contrast with only synchronized animals.

• 한국주조공학회지
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• 제23권5호
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• pp.276-285
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• 2003

In the undercooled melt of $Pd_{40}Cu_{30}Ni_{10}P_{20}$ alloy, the solidification behavior including nucleation and growth of crystals at the micrometer level has been observed in-situ by use of a confocal scanning laser microscope combined with an infrared image furnace. The $Pd_{40}Cu_{30}Ni_{10}P_{20}$ alloy specimens were cooled from the liquid state to glass transition temperature. 575 K, at various cooling late under a helium gas flow. According to the cooling rate, the morphologies of the solidification front are changed among various types, irregular jog like front, columnar dendritic front, cellular grain, star like shape jog and fine grain, etc. The velocities of the solid-liquid interface are measured to be $10^{-5}{\sim}10^{-8}$ m/s which are at least two orders higher than the theoretical crystal growth rates. Combining the morphologies observed in terms of cooling rates and their solidification behaviors, we conclude that phase separation takes place in the undercooled molten $Pd_{40}Cu_{30}Ni_{10}P_{20}$ alloy. The continuous cooling transformation (CCT) diagram was constructed from solidification onset time at various linear cooling conditions with different rate. The CCT diagram suggests that the critical cooling rate for glassy solidification is about 1.5 K/s, which is in agreement with the previous calorimetric findings.

• 한국재난정보학회 논문집
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• 제18권4호
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• pp.746-753
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• 2022

연구목적: 본 연구에서는 1960~70년대에 굴착한 식품 저장용 소규모 터널에 대한 안전성 검토와 소규모 터널의 안정성 향상을 위한 방안을 연구목적으로 한다. 연구방법:안전성 시험을 진행하고자 육안검사와 해머테스트를 이용하였으며, 육안검사는 비파괴검사에 실시되는 시험 중 하나이며, 해머테스트는 반발경도법의 타격법 종류 중의 하나이다. 연구결과:조사지역 자료를 통합 분석한 결과 대체로 외관상 양호한 상태를 보이나 파쇄대와 균열된 풍화대가 많으며 또한 작은 균열이 많고 지질 상태가 복잡하여 지속적인 관찰과 주의가 요구되며, 23개의 터널 중 7개는 안전진단이 필요하며, 1개는 붕괴상태, 1개는 안전하며, 14개는 지속적인 관찰과 주의가 필요하다. 결론: 수시로 소규모 터널의 모든 부분을 점검하고 기록으로 남겨야 하며, 본 연구에서 제안한 소규모 터널의 보강 진행시 안정성이 향상될 것으로 기대한다.