• 제목/요약/키워드: content identification

검색결과 641건 처리시간 0.026초

생약복방 드링크제 중 계피성분의 확인 및 계피산의 분리정량 (Identification of Cinnamon Components and Quantitative Determination of Cinnamlc Acid from Crude Drug Drink Preparations)

  • 고성룡;김나미
    • Journal of Ginseng Research
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    • 제15권1호
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    • pp.1-5
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    • 1991
  • For the quality control of crude drug drink preparations, methods for identification of cinnamon components and quantitative determination of cinnamic acid were established. Cinnamon components were identified by TLC with benzene/ethyl acetate (1:1, v/v) on silica gel plate by spraying p-anisaldehyde-sulfuric acid. Cinnamic acid contents were determined at UV 280 nm by HPLC on $\mu$-Bondapak Cls column with acetonitrile/water/acetic acid (40:60:2, v/v). Recoveries of cinnamic acid in three crude drug drink preparations were between 84.1-90.2% compared to the content of the cinnamon extract.

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Construction of DNA marker for traceability in Hanwoo

  • 권재철;최유미;이성원;여정수;이제영
    • 한국데이터정보과학회:학술대회논문집
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    • 한국데이터정보과학회 2006년도 PROCEEDINGS OF JOINT CONFERENCEOF KDISS AND KDAS
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    • pp.213-219
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    • 2006
  • Considering all the factors involved in beef production individual identification using DNA marker testing is the most appropriate solution to give all the breeders' information to the consumers. After taking into account the genealogical information from the Hanwoo, only animals that did not share some parent or grandparent were analysed 33 from the 305 initially sampled. Ten major microsatellite markers were selected from allele amplified and their frequencies, H(Heterozygosity) and PIC(Polymorphism information content) with Hardy-Weinberg equilibrium. Next, in order to evaluate the power of the markers selected on the individual animal identification, the match probability(MP) and the relatedness coefficient(R) were computed.

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Identification of Adenosine Deaminase Inhibitor-producing Bacterium Isolated from Soil

  • SHIN, YONG KOOK;YONG-HA PARK;JAE-DONG LEE;HONG-KI JUN
    • Journal of Microbiology and Biotechnology
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    • 제7권1호
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    • pp.32-36
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    • 1997
  • An adenosine deaminase inhibitor-producing bacterium was isolated from soil. An isolate exhibiting high adenosine deaminase inhibitory activity, was designated J-89, and classified as a strain of Bacillus subtilis on the basis of its morphological, phenotypic characteristics, the menaquinone content and cellular fatty acid composition. To confirm the taxonomic position of the strain we need more information such as DNA-DNA homology and other chemotaxonomic characteristics. In this paper we provisionally named strain J-89 as Bacillus sp. J-89 pending further chemotaxonomic study and analysis of adenosine deaminase inhibitor.

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Genetically Independent Tetranucleotide to Hexanucleotide Core Motif SSR Markers for Identifying Lentinula edodes Cultivars

  • Saito, Teruaki;Sakuta, Genki;Kobayashi, Hitoshi;Ouchi, Kenji;Inatomi, Satoshi
    • Mycobiology
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    • 제47권4호
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    • pp.466-472
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    • 2019
  • For the purpose of protecting the rights of Lentinula edodes breeders, we developed a new simple sequence repeat (SSR) marker set consisting only of genetically independent tetranucleotide or longer core motifs. Using available genome sequences for five L. edodes strains, we designed primers for 13 SSR markers that amplified polymorphic sequences in 20 L. edodes cultivars. We evaluated the independence of every possible marker pair based on genotype data. Consequently, eight genetically independent markers were selected. The polymorphic information content values of the markers ranged from 0.269 to 0.764, with an average of 0.409. The markers could distinguish among 20 L. edodes cultivars and produced highly repeatable and reproducible results. The markers developed in this study will enable the precise identification of L. edodes cultivars, and may be useful for protecting breeders' rights.

Cholesterol Oxidase를 생산하는 방선균분리주 HSL-613의 동정 (Identification of the Streptomyces Strain HSL-613 Producing Cholesterol Oxidase)

  • 이홍수;이인애;최용경;이희구;이근철;박용하;오태광;최인성;정태화
    • 한국미생물·생명공학회지
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    • 제22권4호
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    • pp.373-381
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    • 1994
  • An actinomycete strain, HSL-613 was isolated -from soil and identified by International Streptomyces Project (ISP) and chemotaxonomic methods. The spore chain of the strain HSL-613 appears in a spiral shape, and its spores are spherical shape with smooth surface. The cell wall contains LL-diaminopimelic acid (DAP). Menaquinone MK-9 (H$_{6}$, H$_{8}$) and iso- and anteiso-branched fatty acids were detected from whole cell extract. Sugars identified from whole cell extract include galactose, glucose, mannose and ribose, which are distinct from general sugar patterns of Streptomyces. Average G+C content in the chromosome is 59%. 5S rRNA of HSL-613 consists of 120 nucleotides as determined by comparing with that of a type strain Streptomyces griseus subsp. KCTC 9080. Through morphological, physiological, and chemical characterization, HSL-613 was identified and named as Streptomyces sp. HSL-613.

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일반 초중등학교에서 과학영재를 위한 과학 심화학습 프로그램의 탐색 (An Exploration of Science Enrichment Learning Program for the Science Gifted in General Elemetary and Secondary School)

  • 김명환
    • 영재교육연구
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    • 제8권1호
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    • pp.7-38
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    • 1998
  • In order to explore the science enrichment learning program for the science gifted in general elemetary and secondary schoo1,the identification of the science gifted, gifted education methods and science education cumculums were investigated through literature survey. Admitting the Renzulli's enrichment triad model and three dmensional scienctific factors as context, process and content, the snow ball program was explored. Identification methods and science education cumculum of enrichment learning for the science gifted were presented. In the snow ball program, it was presented that each factor of three dimensional scienctific factors was focused in the different ways due to the levels or the ages of students. The examples of the snow ball program and science education cumculum of enrichment learning for the science gifted were illustrated.

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재해석 기법을 이용한 동적 구조시스템의 System Identification (System Identification of Dynamic Systems Using Structural Reanalysis Method)

  • 한경봉;박선규;김형열
    • 한국콘크리트학회:학술대회논문집
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    • 한국콘크리트학회 2004년도 추계 학술발표회 제16권2호
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    • pp.421-424
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    • 2004
  • Model updating is a very active research field, in which significant efforts has been invested in recent years. Model updating methodologies are invariably successful when used on noise-free simulated data, but tend to be unpredictable when presented with real experimental data that are-unavoidably-corrupted with uncorrelated noise content. In this paper, Reanalysis using frequency response functions for correlating and updating dynamic systems is presented. A transformation matrix is obtained from the relationship between the complex and the normal frequency response functions of a structure. The transformation matrix is employed to calculate the modified damping matrix of the system. The modified mass and stiffness matrices are identified from the normal frequency response functions by using the least squares method. Full scale pseudo dynamic pier test is employed to illustrate the applicability of the proposed method. The result indicate that the damping matrix of correlated finite element model can be identified accurately by the proposed method. In addition, the robustness of the new approach uniformly distributed measurement noise is also addressed.

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BCON : Blockchain-based Content Management Service Using DID

  • Kim, Hye-Won;Lee, Young-Eun;Kwon, Min-Ho;Lee, Myung-Joon
    • 한국컴퓨터정보학회논문지
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    • 제26권6호
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    • pp.97-105
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    • 2021
  • 본 논문에서는 개인 스스로가 자신의 신원을 인증하여 개인 콘텐츠를 안전하게 보관, 콘텐츠에 대한 자신의 소유권을 신뢰성 있게 보장받을 수 있는 서비스인 BCON을 제안한다. 데이터 위변조가 불가능한 블록체인을 기반으로 탈중앙화된 신원확인 서비스를 지원하는 DID 기술은 사용자가 자신의 정보를 선택적으로 제공하여 자신의 신원정보에 대한 주권을 강화하고 스스로 개인 정보에 대한 통제가 가능하다. BCON은 블록체인에 사용자가 지정한 콘텐츠에 대한 정보를 저장하고 DID 기술을 기반으로 사용자 신원을 인증하여, 사용자가 개인 콘텐츠를 안전하게 분산 데이터베이스에 업로드하고 다운로드하는 기능을 제공한다. BCON은 콘텐츠 서비스 검증자, 콘텐츠 보관 서비스, 콘텐츠 매니지먼트 컨트랙트 및 사용자 어플리케이션으로 구성되며, 서비스 권한 관리를 위한 DID 레지스트리를 운영한다.

모유수유 적응 측정 도구 개발 (Development of a Breast Feeding Adaptation Scale (BFAS))

  • 김선희
    • 대한간호학회지
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    • 제39권2호
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    • pp.259-269
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    • 2009
  • Purpose: The aim of this study was to develop a breast feeding adaptation scale (BFAS) to evaluate adaptation to breastfeeding for breastfeeding mothers and their infants and to test the validity and reliability of the instrument. Methods: The study was conducted as follows: application of the conceptual framework, identification of the content domains, items generation, and test of validity and reliability. In order to test validity and reliability, two panels of experts reviewed items and subcategories of the preliminary questionnaire and then data were collected from 329 mothers who were up to 4 weeks postpartum and breastfeeding. Descriptive statistics, t-test, factor analysis, and Cronbach's alpha were used to analyze the data. Results: The conceptual framework was based on the Roy adaptation model. The content domains were developed via literature review, review of instruments, and data acquired from the interviews of breastfeeding mothers and nurses. A total of 69 items belonging to 8 domains were generated. A reduction to 44 preliminary items was accomplished through content validity analysis. Factor analysis extracted 8 factors with a total of 27 items on a 5-point Likert scale. Content validity, construct validity, criterion validity, and reliability of the BFAS were established. Conclusion: The newly developed BFAS is a reliable and valid instrument with which the adaptation of breastfeeding mothers and their infants to the breastfeeding behavior can be evaluated.

Quality Comparison of Activated Carbon Produced From Oil Palm Fronds by Chemical Activation Using Sodium Carbonate versus Sodium Chloride

  • MAULINA, Seri;HANDIKA, Gewa;Irvan, Irvan;ISWANTO, Apri Heri
    • Journal of the Korean Wood Science and Technology
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    • 제48권4호
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    • pp.503-512
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    • 2020
  • Using Na2CO3 versus NaCl as chemical activator, we compared the quality of activated carbon produced from oil palm fronds as raw material. These activators were selected for comparison because both are readily available and are environmentally friendly. In the manufacturing, we used Indonesian National Standard (SNI 06-3730-1995) parameters. For the quality comparison, we determined activated-carbon yield, moisture, ash, volatiles, and fixed-carbon contents; and adsorption capacity of iodine. The best characteristics, assessed by morphological surface analysis and Fourier transform infrared (FTIR) spectral analysis, were observed in the carbon activated by Na2CO3 at an activator concentration of 10% and carbonization temperature of 400 ℃. The results were as follows: activated-carbon yield, 84%; water content, 8.80%; ash content, 2.20%; volatiles content, 14.80%; fixed-carbon content, 68.60%; and adsorption capacity of iodine, 888.51 mg/g. Identification using the FTIR spectrophotometer showed the presence of the functional groups O-H, C=O, C=C, C-C, and C-H in the Na2CO3-activated carbon.