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Studies on the Hydrolysis of Tannin in Food by Fungal Tannase -Part 1. Screening test of Molds on the Production of Acorn tannin hydrolyzing Enzyme and studies on the cultural conditions of selected strain- (미생물(微生物) Tannase에 의(依)한 식품(食品)의 Tannin 성분(成分) 분해(分解)에 관(關)한 연구(硏究) -제(第)1보(報) 한국산(韓國産) 도토리 Tannin 분해(分解) 효소(酵素) 생산균주(生産菌株)의 분리(分離)와 효소(酵素) 생산(生産)을 위한 배양조건(培養條件)의 검토(檢討)-)

  • Chae, Soo-Kyu;Yu, Tai-Jong
    • Korean Journal of Food Science and Technology
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    • v.5 no.4
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    • pp.258-267
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    • 1973
  • Chemical analysis and enzymatic hydrolysis of certain Korean acorns were performed in order to make use of the local acorns. Exclusion of tannin from acorns is aided in the processing. Studies of these were undertaken and the results obtained are summarized as follows; 1. Several Korean acorns were used to analyze their proximate composition, tannin and major inorganic principles. The content of acorn tannin was found to be 6.5 to 7.5%. 2. An Attempt was made to screen suitable strains in order to make acorn-tannin-hydrolyzing enzyme accumulated in the culture broth, and Aspergillus flavus and Asp. sp. AN-11, which showed in their culture broths, were obtained from the contaminated acorns. 3. Cultural measures for the strains of Aspergillus flavus and Asp. sp. AN-11 for an improved tannase production and optimal conditions were determined.

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A Survey on the Actual Wearing Condition of Lab Gowns (실험 가운의 착용 실태 조사)

  • 최정화;김소영;이주영
    • Journal of the Korean Society of Clothing and Textiles
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    • v.28 no.1
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    • pp.172-181
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    • 2004
  • The purpose of this study was to develop functional lab gowns in the view of safety and work efficiency. As the first step of this study, we surveyed on satisfaction rates, preferences and inconvenience items in regard of lab gowns. The data was obtained from 138 graduate students specialized in science and 113 medical students. Respondents answered that the first purpose of wearing lab gown was to protect body from danger. Wearing frequency followed with ‘always’, ‘as possible as’, and then ‘sometimes’. Forty-five percentage of respondents answered that they didn't wear lab gowns often in summer because of hot weather. The contaminated or damaged parts of lab gowns followed with cuffs, abdomen, thigh, lower part, and then chest. Washing frequency followed with ‘once a month’, ‘once per six months’, and then ‘more than once a week’ There were significant differences in normal wearing frequency, wearing frequency by season and washing frequency due to major. There were no significant difference by sex and major in all questions. In regard of the protective capacity of current lab gowns, most respondents answered that ‘It's normal’(60%), ‘It's enough’(21%), so that they didn't show much dissatisfaction. Forty-three percentage of respondents were not satisfied with cuffs. Respondents answered that the cuffs were in danger and inconvenient because of broad cuffs. In regard of color, preference for white were high and followed with ivory, light sky, light green. and then light pink. Materials of current lab gowns were cotton/polyester or polyester 100%. Sixty-eight percentage of respondents were satisfied with that and others were not. The reasons for dissatisfactions were as follows; those materials were not protective to chemicals thoroughly enough. Cotton/polyester materials were heavy. Wearing sensation of polyester 100% was not good.

Determination of Aflatoxin M1 and Heavy Metals in Infant Formula Milk Brands Available in Pakistani Markets

  • Akhtar, Saeed;Shahzad, Muhammad Arif;Yoo, Sang-Ho;Ismail, Amir;Hameed, Aneela;Ismail, Tariq;Riaz, Muhammad
    • Food Science of Animal Resources
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    • v.37 no.1
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    • pp.79-86
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    • 2017
  • Aflatoxin $M_1$ ($AFM_1$) after its bioconversion from aflatoxin $B_1$ in animal liver becomes the part of milk while heavy metals get entry into milk and milk products during handling in the supply chain. Aflatoxin $M_1$ and heavy metals being toxic compounds are needed to be monitored continuously to avoid any ailments among consumers of foods contaminated with such toxicants. Thirteen commercially available infant formula milk (IFM) brands available in Pakistani markets were analyzed for the quantitative determination of $AFM_1$ and heavy metals through ELISA and atomic absorption spectrophotometer, respectively. $AFM_1$ was found positive in 53.84% samples while 30.76% samples were found exceeding the maximum EU limit i.e. $0.025 {\mu}g/kg$ for $AFM_1$ in IFM. Heavy metals lead (Pb) and cadmium (Cd) were found below the detection limits in any of the sample, whereas the concentrations of iron (Fe), zinc (Zn) and nickel (Ni) ranged between 45.40-97.10, 29.72-113.50 and <$0.001-50.90 {\mu}g/kg$, respectively. The concentration of Fe in all the tested brands was found in normal ranges while the concentrations of Zn and Ni were found exceeding the standard norms. Elevated levels of $AFM_1$, Zn and Ni in some of the tested IFM brands indicated that a diet completely based on these IFM brands might pose sever health implications in the most vulnerable community i.e., infants.

Proteomic Analysis of Polycyclic Aromatic Hydrocarbons (PAHs) Degradation and Detoxification in Sphingobium chungbukense DJ77

  • Lee, Soo Youn;Sekhon, Simranjeet Singh;Ban, Yeon-Hee;Ahn, Ji-Young;Ko, Jung Ho;Lee, Lyon;Kim, Sang Yong;Kim, Young-Chang;Kim, Yang-Hoon
    • Journal of Microbiology and Biotechnology
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    • v.26 no.11
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    • pp.1943-1950
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    • 2016
  • Polycyclic aromatic hydrocarbons (PAHs) are commonly present xenobiotics in natural and contaminated soils. We studied three (phenanthrene, naphthalene, and biphenyl) xenobiotics, catabolism, and associated proteins in Sphingobium chungbukense DJ77 by two-dimensional gel electrophoresis (2-DE) analysis. Comparative analysis of the growth-dependent 2-DE results revealed that the intensity of 10 protein spots changed identically upon exposure to the three xenobiotics. Among the upregulated proteins, five protein spots, which were putative dehydrogenase, dioxygenase, and hydrolase and involved in the catabolic pathway of xenobiotic degradation, were induced. Identification of these major multifunctional proteins allowed us to map the multiple catabolic pathway for phenanthrene, naphthalene, and biphenyl degradation. A part of the initial diverse catabolism was converged into the catechol degradation branch. Detection of intermediates from 2,3-dihydroxy-biphenyl degradation to pyruvate and acetyl-CoA production by LC/MS analysis showed that ring-cleavage products of PAHs entered the tricarboxylic acid cycle, and were mineralized in S. chungbukense DJ77. These results suggest that S. chungbukense DJ77 completely degrades a broad range of PAHs via a multiple catabolic pathway.

Effect of Kaolin on Arsenic Accumulation in Rice Plants (Oryza Sativa L.) Grown in Arsenic Contaminated Soils

  • Koonsom, Titima;Inthorn, Duangrat;Sreesai, Siranee;Thiravetyan, Paitip
    • Environmental Engineering Research
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    • v.19 no.3
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    • pp.241-245
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    • 2014
  • The As accumulation in part of roots, shoots, husks and grains of rice plants was significantly decreased with the increasing dosage of kaolin addition from 0.5% to 10% w/w. Kaolin addition could reduce As accumulation in rice plants, which mainly could be attributed to the formation of stable crystalline Al oxides bound As that decreased the available As in soil with decreased As accumulation in rice plants. The pH values of the soils did not change significantly when amended with kaolin. The pH values of the soils was neural that proper to adsorb of arsenic with $Al_2O_3$. Arsenic tends to adsorb with $Al_2O_3$ at acid neutral pH and with desorbing at alkaline pH. The dry weight of rice plant was significantly increased with the increasing dosage of kaolin addition from 2.5% to 10% w/w. The highest dry weight of rice plants was 6.67 g/pot achieved at kaolin addition of 10% w/w with about 13% increasing over the control, which was probably attributed to the highest As concentration formation with kaolin at this dosage. The results of this study indicated that kaolin has the potential to reduce As accumulation in rice plants and enhance the dry weight of rice plants.

Transfer of Arsenic and Heavy Metals from Soils to Rice Plant under Different Drainage Conditions (논토양 배수조건에 따른 비소 및 중금속의 용출 및 벼 전이특성)

  • Koh, Il-Ha;Kim, Jungeun;Kim, Gi Suk;Ji, Won Hyun
    • Journal of Soil and Groundwater Environment
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    • v.22 no.6
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    • pp.12-21
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    • 2017
  • A pot experiment was conducted to investigate the transfer of As and cationic heavy metals (Fe, Mn, Zn, Cd and Pb) from soil to rice plant in soil condition with submerged and drained. During the ninety-day monitoring period for soil solution, solubility of reducible elements such as As, Fe and Mn in submerged condition were higher than that of Zn. On the contrary, concentration of Zn in drained condition was higher than that of reducible elements. The concentration of As, Cd, Pb and Zn in rice plant (root, stem, leaf and grain) showed similar pattern with soil solution. The As concentration in each part of rice plant, which cultivated in drained condition, measured 56%~94% lower than those in submerged condition. However, the contents of cationic heavy metals (Cd, Pb and Zn) were represented the opposite result with As. These results are due to mobility of As and cationic heavy metals under different soil drainage conditions which represent oxidation and reduction. Thus soil drainage control can be used as acceptable passive treatment methods to reduce transfer of inorganic contaminants from soil to rice plant. However more detailed examination on soil condition conversion is needed, because yield of rice was decreased when it cultivated in drained condition only. It also needed when soil is contaminated by As and cationic heavy metal because single drainage condition cannot reduce transfer of both kinds of contaminants all.

An Extraction of Solar-contaminated Energy Part from MODIS Middle Infrared Channel Measurement to Detect Forest Fires

  • Park, Wook;Park, Sung-Hwan;Jung, Hyung-Sup;Won, Joong-Sun
    • Korean Journal of Remote Sensing
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    • v.35 no.1
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    • pp.39-55
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    • 2019
  • In this study, we have proposed an improved method to detect forest fires by correcting the reflected signals of day images using the middle-wavelength infrared (MWIR) channel. The proposed method is allowed to remove the reflected signals only using the image itself without an existing data source such as a land-cover map or atmospheric data. It includes the processing steps for calculating a solar-reflected signal such as 1) a simple correction model of the atmospheric transmittance for the MWIR channel and 2) calculating the image-based reflectance. We tested the performance of the method using the MODIS product. When compared to the conventional MODIS fire detection algorithm (MOD14 collection 6), the total number of detected fires was improved by approximately 17%. Most of all, the detection of fires improved by approximately 30% in the high reflection areas of the images. Moreover, the false alarm caused by artificial objects was clearly reduced and a confidence level analysis of the undetected fires showed that the proposed method had much better performance. The proposed method would be applicable to most satellite sensors with MWIR and thermal infrared channels. Especially for geostationary satellites such as GOES-R, HIMAWARI-8/9 and GeoKompsat-2A, the short acquisition time would greatly improve the performance of the proposed fire detection algorithm because reflected signals in the geostationary satellite images frequently vary according to solar zenith angle.

A Study of the Mask and Hand Contamination in Dental Clinic (치과에서의 마스크 및 손의 미생물 오염정도 비교)

  • Pyo, Eunji;Lee, Kyunghee
    • Journal of The Korean Society of Integrative Medicine
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    • v.7 no.3
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    • pp.85-94
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    • 2019
  • Purpose: The purpose of this study was to observe the degree of mask contamination in dental hygienist for general and oral bacteria and to identify areas of mask contamination after treatment. Methods: Masks were collected with every fifty dental hygienists who currently working in the department of preventive dentistry, prosthodontics, and orthodontics in Busan. The mask bacteria were collected in specific upper and side parts of the mask. Hand germs were collected using sterile cotton swabs, and then placed in a sterile conical tube. These were transferred to the laboratory. Hand germs and mask bacteria were incubated with nutrient broth (NB) and brain heart infusion broth (BHI) for 24 hrs and each cultured with NB and BHI plate at $37^{\circ}C$ for 48 hrs. Collected data were analyzed using the SPSS Window 20. Results: The number of bacteria was observed in the order of the department of preventive dentistry ($10.1{\times}10^5CFU/ml$), prosthodontics ($14.7{\times}10^5CFU/ml$), and orthodontics ($23.3{\times}10^5CFU/ml$) in the hand. In general bacteria, the difference of contamination was seen by the parts of the mask, but there was no significant difference. However, the oral bacteria were observed highly contaminated upper part of the mask in preventive dentistry. The mask contamination according to the medical departments was observed. Especially, the contamination of mask in preventive dentistry was significantly higher than other departments in oral bacteria. Conclusion: This study suggested that correct mask replacement and recognition of contamination areas can contribute to the prevention of infectious disease. and it would be necessary to increase hand hygiene performance to prevent cross-infection with masks. Also, this study may give an idea for making guidelines for mask management and supporting to establish clear criteria for the education program of personal protective equipment.

Identification of Sapstain Fungi on Weathered Wooden Surfaces of Buildings at Jangheung and Jeju Island

  • YUN, Jeonghee;SHIN, Hee Chang;HWANG, Won Joung;YOON, Sae-Min;KIM, Yeong-Suk
    • Journal of the Korean Wood Science and Technology
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    • v.49 no.6
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    • pp.591-601
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    • 2021
  • Recently it is trend to increase wood use as carbon neutral materials, there is recognized to need necessarily durability improvement of wooden building. It is very rare to report existing on the identification of isolates causing discoloration in domestic weathered wooden building used for long period. The objective of this study was identification of fungi that cause discoloration on the exteriors of weathered domestic wooden buildings in the southern part of South Korea. Our findings can be helpful to establish protection technology for weather deterioration of domestic wooden buildings. Wood chip samples presumed to be contaminated with sapstain fungi were collected from the surface of wooden members used in wooden buildings at Jangheung, Jeollanam-do (two locations, #13 and #14), and Jeju Island (two locations, #31 and #33). The growth of microorganisms was confirmed by performing culture tests for the collected samples, and fungi were isolated, purified, and identified. The results indicated that the fungal strains isolated from wooden buildings #13 and #14 at Jangheung, Jeollanam-do, were 99.83% and 100% homologous to Aureobasidium melanogenum, respectively. For wooden building #31 at Jeju Island (two locations), the fungal strain isolated was 100% homologous to A. melanogenum, which is the same species isolated from the wooden buildings at Jangheung. The fungal strain isolated from wooden building #33 (Jeju Island) had 99.83% homology with A. pullulans, which is commonly found in wood degraded by weather or ultraviolet rays. Our findings can be utilized as a basis for establishing protection technology in domestic wooden buildings.

Development of Temporary Preservation Method for Small Scale Dairy Farm Milk by $H_2O$$_2$ Catalase Treatment (Part 1) Bactericidal Effect of Hydrogen Peroxide and Its Stability in Milk ($H_2O$$_2$-Catalase처리에 의한 소규모 목장우유의 일시적 보존법의 개발 (제1보) 우유에 있어서 과산화수소의 살균효과 및 안정성)

  • Park, I.S.;Pack, M.Y.
    • Microbiology and Biotechnology Letters
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    • v.5 no.3
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    • pp.113-118
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    • 1977
  • Into the precontaminated farm milk hydrogen peroxide ($H_2O$$_2$) was added at the concentrations ranging from 0.01% to 0.05% and kept at 3$0^{\circ}C$ for 16 hours with periodical determinations for viable counts, residual $H_2O$$_2$, and lactic acid. Under the tested conditions the initial level of contaminated bacteria could be arrested from growing at least for 8, 12, and 16 hours by treating the milk with 0.01, 0.02. and 0.03 per cent of $H_2O$$_2$, respectively. Furthermore, when the $H_2O$$_2$concentrations ware limited within the level of 0.03 Per cent the added $H_2O$$_2$was completely decomposed within 12 hours without the aid of external catalase and the decomposition time decreased in parallel with the $H_2O$$_2$ concentrations. A safer use of $H_2O$$_2$for preserving farm milk temporarily by limiting its concentration has been discussed.

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