Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Proteomic Analysis of Polycyclic Aromatic Hydrocarbons (PAHs) Degradation and Detoxification in Sphingobium chungbukense DJ77

  • Lee, Soo Youn (School of Biological Sciences, Chungbuk National University) ;
  • Sekhon, Simranjeet Singh (School of Biological Sciences, Chungbuk National University) ;
  • Ban, Yeon-Hee (School of Biological Sciences, Chungbuk National University) ;
  • Ahn, Ji-Young (School of Biological Sciences, Chungbuk National University) ;
  • Ko, Jung Ho (College of Veterinary Medicine, Western University of Health Sciences) ;
  • Lee, Lyon (College of Veterinary Medicine, Western University of Health Sciences) ;
  • Kim, Sang Yong (Department of Food Science and Biotechnology, Shin Ansan University) ;
  • Kim, Young-Chang (School of Biological Sciences, Chungbuk National University) ;
  • Kim, Yang-Hoon (School of Biological Sciences, Chungbuk National University)
  • Received : 2016.06.03
  • Accepted : 2016.07.21
  • Published : 2016.11.28
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Abstract

Polycyclic aromatic hydrocarbons (PAHs) are commonly present xenobiotics in natural and contaminated soils. We studied three (phenanthrene, naphthalene, and biphenyl) xenobiotics, catabolism, and associated proteins in Sphingobium chungbukense DJ77 by two-dimensional gel electrophoresis (2-DE) analysis. Comparative analysis of the growth-dependent 2-DE results revealed that the intensity of 10 protein spots changed identically upon exposure to the three xenobiotics. Among the upregulated proteins, five protein spots, which were putative dehydrogenase, dioxygenase, and hydrolase and involved in the catabolic pathway of xenobiotic degradation, were induced. Identification of these major multifunctional proteins allowed us to map the multiple catabolic pathway for phenanthrene, naphthalene, and biphenyl degradation. A part of the initial diverse catabolism was converged into the catechol degradation branch. Detection of intermediates from 2,3-dihydroxy-biphenyl degradation to pyruvate and acetyl-CoA production by LC/MS analysis showed that ring-cleavage products of PAHs entered the tricarboxylic acid cycle, and were mineralized in S. chungbukense DJ77. These results suggest that S. chungbukense DJ77 completely degrades a broad range of PAHs via a multiple catabolic pathway.

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References

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