• Journal of Forest and Environmental Science
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• 제24권2호
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• pp.79-88
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• 2008

Twenty-eight of 44 Indian Ceropegia species are endemic and their survival is threatened. As a step towards conservation, we implied in vitro methods for the sustainable propagule production in C. hirsuta, C. lawii, C. maccannii, C. oculata and C. sahyadrica. Effects of explant, growth regulators, sucrose and photoperiod were studied. High frequency microtuber production was achieved with the seedling-apical buds, grown on MS medium containing 4-6 mg $1^{-1}$ BAP, 3-8% (w/v) sucrose, under continuous illumination. Each microtuber, when subcultured proliferated to form a cluster of secondary microtubers. Every primary and secondary microtuber bore at least one shoot-bud and a root primordium. Each tuber (formed with any of the significantly effective treatments) weighed more than 500 mg, enough to plant directly in non-sterilized soils. Microtubers could be produced and proliferated round the year. Proliferation could be solely attributed to in vitro procedures as these plants bear solitary tubers in vivo. Microtubers could be sprouted in vitro to prepare ready to pot plantlets. As, this novel method succeeded for all five species, though they belong to different eco-physiological backgrounds, we recommend its implementation in the conservation programs for a broader range of Ceropegia species, supported by other integrated strategies.

• Animal cells and systems
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• 제8권Supplyment
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• pp.64-64
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• 2004

• 한국환경과학회:학술대회논문집
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• 한국환경과학회 2002년도 The 10th Anniversary of KENSS International Workshop
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• pp.61-73
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• 2002

• Animal cells and systems
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• 제16권3호
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• pp.230-236
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• 2012

The Korean hare, Lepus coreanus, is an important mammal in ecosystem food chains, and is distributed across the entire Korean peninsula and northeastern China. Polymorphic microsatellite loci were developed using the biotinenrichment technique for use in population genetics studies. Five trinucleotide and four dinucleotide microsatellite loci were selected and tested on 22 Korean hare specimens collected from Gangwon Province and Gyeongsangbuk Province in South Korea. The number of alleles across the two sampling regions ranged from three to nine with a mean of 6.1. Mean observed and expected heterozygosities and polymorphic information content were 0.540, 0.627 and 0.579, respectively. Only one locus, Lc06, showed departure from Hardy-Weinberg equilibrium after applying the Bonferroni correction. Four microsatellites, Lc01, Lc03, Lc12, and Lc19, satisfied the criteria to serve as a core set of markers recommended for population genetics studies. These new microsatellite markers will be widely applicable to future genetic studies for management and conservation of the Korean hare and related species, including assessment of the genetic diversity and population structure of L. coreanus.

• 환경생물
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• 제26권4호
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• pp.343-348
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• 2008

본 연구는 ARCS의 학습동기모델 전략(주의집중, 관련성, 자신감, 만족감)을 중학교 환경교과서의 [환경보존]단원에 적용하기 위하여 다른 수업 방식과 비교한 것이다. 이를 위하여 부산광역시에 소재하는 중학교 2학년 135명에게 적용하기 위하여 학생들을 수업전략에 따라서 그룹으로 나누었다. 첫 번째 그룹은 전통주의 방식에 의한 수업을 받는 학생들로 구성되어 있었으며, 두 번째는 구성주의 수업방식을 받는 학생, 세 번째는 ARCS전략을 사용한 구성주의 수업방식을 받는 학생들로 이루어졌다. 사전 인지 검사를 통해 적용하기 전의 선인지를 조사하였다. 학습이 이루어진 후 학생들의 과학적 개념의 인지, 학업성취도, 학습동기 등을 조사하였다. ARCS전략을 사용한 구성주의 수업방식을 받는 학생들의 학업성취도는 평균적으로 다른 그룹의 학생들보다 높았으며 유의한 차이를 나타내었다. 따라서 ARCS전략을 사용한 구성주의 수업방식이 다른 두 그 수업방식에 비해 환경보전 단원에 대해 보다 효과적인 학습동기를 유발한다는 것이 밝혀졌다.

• Journal of Microbiology
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• 제45권6호
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• pp.479-484
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• 2007

Bacterial communities at 10 cm, 100 cm, and 200 cm depths in a 100-year-old lead-zinc tailing heap were evaluated by constructing 16S rRNA gene libraries. In total, 98 operational taxonomic units (OTUs) were identified from 193 clones at a 3% sequence difference level. The OTU number and species richness decreased with the depth. Species composition was significantly different between the three libraries. Fifty-seven percent of the examined clones were Acidobacteria and 27% belonged to Proteobacteria. Other sequences included Chloroflexi, Firmicutes, Chlamydiae, Actinobacteria, Gemmatimonadetes, Nitrospira, and three unclassified OTUs. Alphaproteobacteria, Betaproteobacteria, Gammaproteobacteria, Firmicutes, and Actinobacteria were mainly distributed in the rhizosphere of naturally colonizing plants; however, Deltaproteobacteria, Acidobacteria, and Chloroflexi tended to inhabit the deeper tailings (below the 100 cm-depth).

• 한국균학회지
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• 제40권3호
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• pp.132-135
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• 2012

제주도와 완도에서 채집한 총 5종의 자생란의 뿌리에서 미동정 1종을 포함한 총 4종의 균을 순수분리하였으며, 추출한 DNA를 ITS지역을 증폭하여 분자적 동정하였다. 이중 3종이 Tulasnella속이었으며, 1종은 Sebacina vermifera로 일반적인 난균근균이었다. 제주도와 완도에서 모두 채집한 보춘화(Cymbidium goeringii)의 경우 제주도에서 채집한 뿌리에서는 Sebacina vermifera가, 완도에서 채집한 뿌리에서는 Tulasnella calospora가 분리되어 같은 종의난과 식물에서도 서식지에 따라 공생하는 난균근균이 다를 수 있음을 확인하였다.

• 식물분류학회지
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• 제47권1호
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• pp.6-10
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• 2017

Microsatellite markers were isolated for Daphne kiusiana var. kiusiana (Thymelaeaceae), an evergreen broad-leaved shrub endemic to Korea and Japan. Because its populations in Jeju Island are morphologically controversial, and consistently threatened by anthropogenic pressures, taxonomic delimitation and conservation effort are required at the genetic level. We developed 21 polymorphic microsatellite loci from Next Generation Sequencing data. The primer set included di-, tri-, and tetra-nucleotide repeats. Variability in the markers was tested for 80 individuals of D. kiusiana from three natural populations in Jeju Island and Japan. Among the 21 loci, three were unavailable for population JKJU of Japan. The Neighbor-Joining tree based on microsatellite markers described here classified the three populations into two groups according to geographical or morphological traits. These will be a powerful genetics tool for determining the taxonomic boundary and establishing suitable conservation strategies for D. kiusiana in Jeju Island.

• 헤리티지:역사와 과학
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• 제35권
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• pp.120-137
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• 2002

It has not been a long time since we recognize that a word 'DNA' is not unfamiliar with us. Development of biology give us so much of benefits of civilization and so we call the 21th century as 'biological period'. It has not been a long time that archeology made contact with biology. With biological development, DNA typing analysis has been accomplished extensively since 1990's. We know through mitochondrial DNA base sequencing analysis that the Neanderthal man is not the origin of the human race and ancient human race set out from Africa. Biological science technology, which is polymerase chain reaction(PCR) or electrophoresis etc., made these results possible. A contact between biology, especially genetics, and archeology is getting accomplished through these current. If genetics keep in contact with archeological foundation, we know not only about ancient populations in the Korean Peninsula, but also origin of human race. This field is so-called 'DNA Archeology'. This field is of help to person identification and children discrimination as like a forensic science. We make every effort for great possibilities from co-ownership of these two fields and these fields needs to convert a recognition, especially.

• 환경영향평가
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• 제15권5호
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• pp.339-355
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• 2006

In this paper, we reviewed the assessment criteria and conservation value used to evaluate vegetation. The Degree of Green Naturality (DGN) and the Grade of Vegetation Conservation (GVC) are both sets of criteria that estimate the degree of human disturbance or natural value of vegetation. The criteria are extensively used in decision-making about the natural environment conservation and environment impact assessment. Sometimes, social issues can rise because the criteria are not clear. This study aims to evaluate the criteria based on the many aspects of the related literatures and suggest reasonable revised criteria. In addition, criteria for representing the relative value of valuable vegetation conservation are suggested. The DGN and GVC are essentially same; both have 11 degrees and 5 grades. While the DGN is subdivided into levels of anthropogenic disturbances, the GVC indicates the priority for conserving valuable vegetation. Therefore, the DGN is very useful for assessing land development projects, etc., while the GVC is needed to delineate the Ecological Assessment Map(EAM). In conclusion, it is desirable that both criteria should be used together appropriately.