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Evaluations of Growth and Forage Quality of Sesbania Accessions Adaptable to Korean Environments (국내적응 세스바니아의 생육특성 및 사료가치 평가)

  • Lee, Chang Min;Kim, Young Jin;Ahn, Sol;Hailegioris, Daniel;Lee, Cheong Ae;Yun, Song-Joong
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.64 no.3
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    • pp.278-286
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    • 2019
  • Sesbania, an annual herb, is known for its high forage value and salt tolerance. It has merits as a forage crop that is adaptable to reclaimed land in the Republic of Korea. Therefore, we collected Sesbania genetic resources from the Republic of Korea and other countries, and conducted experiments to evaluate their potential as a forage crop in Korean climate and soil conditions. In the preliminary experiments, 15 genetic resources which were able to set seeds in Korean environment were selected out of a total of 46 collected genetic resources. Among 15 genetic resources, SL13 was the tallest and it was followed by that of SC04, SR01 and SE07. The accessions with the earliest flowering started flowering 101 days after sowing and set seed in early August. Fifteen accessions were evaluated for their salt tolerance at germination stage based on germination rate and growth of germinated seedlings at 0 mM, 150 mM and 300 mM NaCl concentrations. Five genetic resources like SC04, SL13, SS20, SS24 and SR01 were selected to be tolerant to NaCl treatment. Forage value was evaluated based on crude protein, acid detergent fiber, neutral detergent fiber and in vitro dry matter digestibility. The forage value of leaves was significantly higher than that of stems, and the forage value of the stem was slightly better than that of rice straw. The forage value of leaves of all the genetic resources was higher than grade 1 by the American Forage and Grassland Council grade. Among five selected genetic resources, the relative feed value of SC04 was the highest and it was followed by that of SS20, SL13, SS24 and SR01.

Effect of Holding Solution on Vase Life of a New Ornamental Crop Known as Euphorbia jolkinii Boiss. (보존용액 처리가 신 관상식물 암대극(Euphorbia jolkinii Boiss.)의 절화수명에 미치는 영향)

  • Song, Su Jung;Park, Hyung Bin;Kim, Ji Sun;Oh, Hye Jin;Kim, Sang Yong;Jeong, Mi Jin;Lee, Seung Youn
    • Korean Journal of Plant Resources
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    • v.32 no.4
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    • pp.312-317
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    • 2019
  • This study was conducted with the purpose of examining the suitability of Euphorbia jolkinii Boiss. as cut flower, so that it may be introduced as a new ornamental crop. For this purpose, effect of various holding solutions on vase solution uptake rate, vase life, and relative fresh weight of cut flowering branches of E. jolkinii was examined. After harvest, cut branches were treated with 10, 50, and $100mg{\cdot}L^{-1}$ of 8-hydroxyquinoline sulfate (8-HQS), 0.1 and 0.2 mM of silver thiosulfate (STS), Chrysal, and Floralife. The cut branches of E. jolkinii were placed under the environmental conditions maintained at air temperature of $22.6^{\circ}C$, relative humidity of 45%, and 9/15h photoperiod that was controlled using fluorescent lamps (light intensity of $9.89{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$). A holding solution containing $10mg{\cdot}L^{-1}$ 8-HQS was found to be significantly effective for vase solution uptake rate compared to control, $50mg{\cdot}L^{-1}$ 8-HQS, and $100mg{\cdot}L^{-1}$ 8-HQS treatments. However, no significant difference was found in vase life between the branches treated with $10mg{\cdot}L^{-1}$ 8-HQS holding solution and branches of the control group. Increasing holding solution concentrations of STS was found to have negative effect on the vase life of cut E. jolkinii branches. Relative fresh weight of cut E. jolkinii branches were significantly decreased by two commercial holding solutions, Chrysal and Floralife. It is expected that these results would aid further studies on utilization of E. jolkinii as cut flower crop.

Evaluation of Manganese Removal from Acid Mine Drainage by Oxidation and Neutralization Method (산화법과 중화법을 이용한 산성광산배수 내 망간 제거 평가)

  • Kim, Bum-Jun;Ji, Won-Hyun;Ko, Myoung-Soo
    • Economic and Environmental Geology
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    • v.53 no.6
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    • pp.687-694
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    • 2020
  • Two oxidizing agents (KMnO4, H2O2), and one neutralizing agent (NaOH) were applied to evaluate Mn removal in mine drainage. A Mn2+ solution and artificial mine drainage were prepared to identify the Fe2+ influence on Mn2+ removal. The initial concentrations of Mn2+ and Fe2+ were 0.1 mM and 1.0 mM, respectively. The injection amount of oxidizing and neutralizing agents were set to ratios of 0.1, 0.67, 1.0, and 2.0 with respect to the Mn2+ mole concentration. KMnO4 exhibited a higher removal efficiency of Mn2+ than did H2O2 and NaOH, where approximately 90% of Mn2+ was removed by KMnO4. A black MnO2 was precipitated that indicated the oxidation of Mn2+ to Mn4+ after an oxidizing agent was added. In addition, MnO2 (pyrolusite) is a stable precipitate under pH-Eh conditions in the solution. However, relatively low removal ratios (6%) of Mn2+ were observed in the artificial mine drainage that included 1.0 mM of Fe2+. The rapid oxidation tendency of Fe2+ as compared to that of Mn2+ was determined to be the main reason for the low removal ratios of Mn2+. The oxidation of Fe2+ showed a decrease of Fe concentration in solution after injection of the oxidizing and neutralizing agents. In addition, Mn7+ of KMnO4 was reduced to Mn2+ by Fe2+ oxidation. Thus, the concentrations of Mn increased in artificial mine drainage. These results revealed that the oxidation method is more effective than the neutralization method for Mn removal in solution. It should also be mentioned that to achieve the Mn removal in mine drainage, Fe2+ removal must be conducted prior to Mn2+ oxidation.

Simulation of the Ocean Circulation Around Ulleungdo and Dokdo Using a Numerical Model of High-Resolution Nested Grid (초고해상도 둥지격자 수치모델을 이용한 울릉도-독도 해역 해양순환 모의)

  • Kim, Daehyuk;Shin, Hong-Ryeol;Choi, Min-bum;Choi, Young-Jin;Choi, Byoung-Ju;Seo, Gwang-Ho;Kwon, Seok-Jae;Kang, Boonsoon
    • Journal of Korean Society of Coastal and Ocean Engineers
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    • v.32 no.6
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    • pp.587-601
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    • 2020
  • The ocean circulation was simulated in the East Sea and Ulleungdo-Dokdo region using ROMS (Regional Ocean Modeling System) model. By adopting the East Sea 3 km model and the HYCOM 9 km data, Ulleungdo 1 km model and Ulleungdo-Dokdo 300 m model were constructed with one-way grid nesting method. During the model development, a correction method was proposed for the distortion of the open boundary data which may be caused by the bathymetry data difference between the mother and child models and the interpolation/extrapolation method. Using this model, a super-high resolution ocean circulation with a horizontal resolution of 300 m near the Ulleungdo and Dokdo region was simulated for year 2018. In spite of applying the same conditions except for the initial and boundary data, the numerical models result indicated significantly different characteristics in the study area. Therefore, these results were compared and verified by using the surface current data estimated by satellites altimeter data and temperature data from NIFS (National Institute of Fisheries Science). They suggest that in general, the improvement of the one-way grid nesting with the HYCOM data on RMSE, Mean Bias, Pattern correlation and Vector correlation is greater in 300 m model than in the 1 km model. However, the nesting results of using East Sea 3 km model showed that simulations of the 1 km model were better than 300 m model. The models better resolved distinct ridge/trough structures of isotherms in the vertical sections of water temperature when using the higher horizontal resolution. Furthermore, Karman vortex street was simulated in Ulleungdo-Dokdo 300 m model due to the terrain effect of th islands that was not shown in the Ulleungdo 1 km model.

The Heterotrimeric Kinesin-2 Family Member KIF3A Directly Binds to Creatine Kinase B (Heterotrimeric kinesin-2의 KIF3A와 creatine kinase B의 결합)

  • Jeong, Young Joo;Park, Sung Woo;Seo, Mi Kyoung;Kim, Sang-Jin;Lee, Won Hee;Kim, Mooseong;Urm, Sang-Hwa;Lee, Jung Goo;Seog, Dae-Hyun
    • Journal of Life Science
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    • v.31 no.3
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    • pp.257-265
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    • 2021
  • Heterotrimeric kinesin-2 is a molecular motor protein of the kinesin superfamily (KIF) that moves along a microtubule plus-end directed motor protein. It consists of three different motor subunits (KIF3A, KIF3B, and KIF3C) and a kinesin-associated protein 3 (KAP3) that form a heterotrimeric complex. Heterotrimeric kinesin-2 interacts with many different binding proteins through the cargo-binding domain of the KIF3s. The activity of heterotrimeric kinesin-2 is regulated to ensure that the cargo is directed to the right place at the right time. How this regulation occurs, however, remains in question. To identify the regulatory proteins for heterotrimeric kinesin-2, we performed yeast two-hybrid screening and found a specific interaction with creatine kinase B (CKB), which is the brain isoform of cytosolic creatine kinase enzyme. CKB bound to the cargo-binding domain of KIF3A but did not interact with the KIF3B, KIF5B, or KAP3 in the yeast two-hybrid assay. The carboxyl (C)-terminal region of CKB is essential for the interaction with KIF3A. Another protein kinase, CaMKIIa, interacted with KIF3A, but GSK3a did not interact with KIF3A in the yeast two-hybrid assay. KIF3A interacted with GST-CKB-C but not with GSK-CKB-N or GST alone. When co-expressed in HEK-293T cells, CKB co-localized with KIF3A and co-immunoprecipitated with KIF3A and KIF3B but not KIF5B. These results suggest that the CKB-KIF3A interaction may regulate the cargo transport of heterotrimeric kinesin-2 under energy-compromised conditions in cells.

Analysis of Behavior of Seoullo 7017 Visitors - With a Focus on Text Mining and Social Network Analysis - (서울로 7017 방문자들의 이용행태 분석 -텍스트 마이닝과 소셜 네트워크 분석을 중심으로-)

  • Woo, Kyung-Sook;Suh, Joo-Hwan
    • Journal of the Korean Institute of Landscape Architecture
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    • v.48 no.6
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    • pp.16-24
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    • 2020
  • The purpose of this study is to analyze the usage behavior of Seoullo 7017, the first public garden in Korea, to understand the usage status by analyzing blogs, and to present usage behavior and improvement plans for Seoullo 7017. From June 2017 to May 2020, after Seoullo 7017 was open to citizens, character data containing 'Seoullo 7017' in the title and contents of NAVER and·DAUM blogs were converted to text mining and socialization, a Big Data technique. The analysis was conducted using social network analysis. The summary of the research results is as follows. First of all, the ratio of men and women searching for Seoullo 7017 online is similar, and the regions that searched most are in the order of Seoul and Gyeonggi, and those in their 40s and 50s were the most interested. In other words, it can be seen that there is a lack of interest in regions other than Seoul and Gyeonggi and among those in their 10s, 20s, and 30s. The main behaviors of Seoullo 7017 are' night view' and 'walking', and the factors that affect culture and art are elements related to culture and art. If various programs and festivals are opened and actively promoted, the main behavior will be more varied. On the other hand, the main behavior that the users of Seoullo 7017 want is 'sit', which is a static behavior, but the physical conditions are not sufficient for the behavior to occur. Therefore, facilities that can cause sitting behavior, such as shades and benches must be improved to meet the needs of visitors. The peculiarity of the change in the behavior of Seoullo 7017 is that it is recognized as a good place to travel alone and a good place to walk alone as a public multi-use facility and group activities are restricted due to COVID-19. Accordingly, in a situation like the COVD-19 pandemic, more diverse behaviors can be derived in facilities where people can take a walk, etc., and the increase of various attractions and the satisfaction of users can be increased. Seoullo 7017, as Korea's first public pedestrian area, was created for urban regeneration and the efficient use of urban resources in areas beyond the meaning of public spaces and is a place with various values such as history, nature, welfare, culture, and tourism. However, as a result of the use behavior analysis, various behaviors did not occur in Seoullo 7017 as expected, and elements that hinder those major behaviors were derived. Based on these research results, it is necessary to understand the usage behavior of Seoullo 7017 and to establish a plan for spatial system and facility improvement, so that Seoullo 7017 can be an important place for urban residents and a driving force to revitalize the city.

Development of Marine Virus-like Particles Live/Dead Determination Method for the Performance Evaluation of Ballast Water Treatment System (선박평형수처리장치 성능 평가를 위한 해양 바이러스 생사판별 방법 개발)

  • Hyun, Bonggil;Woo, Joo-Eun;Jang, Pung-Guk;Jang, Min-Chul;Lee, Woo-Jin;Bae, Mi-Kyung;Shin, Kyoungsoon
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.22 no.1
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    • pp.431-438
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    • 2021
  • To prepare more stringent regulations for USCG Phase II ballast water management, this study investigated the staining efficiency of SYBR Green I(SGI) and SYBR Gold(SG) on the virus-like particle (VLP). A dye with high staining efficiency was applied to the treated water that was passed through the ballast water treatment system (BWTS). VLP staining was observed most clearly under the 100-fold and 200-fold dilution of the stock solution when the volume of filtered samples was 0.5 mL to 2 mL. The staining efficiency of SGI and SG did not show a significant difference. On the other hand, the green fluorescence of viruses in the sample stained with SGI was more pronounced than in the samples stained with SG (expressed yellow fluorescence), making it easier to observe. The abundance of VLP in the test water and control water treatments that did not pass through the two types of BWTS (electrolysis type, UV + electrolysis type) was approximately 109 - 1010 VLP 100 mL-1. In contrast, no stained VLP was observed in the treated water treatments. Moreover, SGI was confirmed to be effectively stained under various salinity conditions, including seawater, brackish water, and freshwater. Further verification tests and development of staining methods under various BWTS are required, but the SGI staining method is believed to be a good alternative to the VLP live/dead determination of the USCG Phase II type approval test.

Plasma Levels of Cytokines in Patients with Postpartum Depression (산후우울증 환자에서 혈장 Cytokine의 농도변화에 대한 전향적 연구)

  • Lee, Younjung;Kim, Yong-Ku;Kim, Kye-Hyun;Lee, Bun-Hee
    • Korean Journal of Psychosomatic Medicine
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    • v.28 no.2
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    • pp.177-184
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    • 2020
  • Objectives : Postpartum depression is known to occur in 10-15% of mothers. The concentration of cytokine varies depending on stress, depression, pregnancy and general medical conditions. We hypothesized that the concentration of cytokines may be related to reproduction and childbirth, and that women with postpartum depression would show alterations in cytokines levels. Methods : A total of 104 pregnant women were selected as subjects, and 60 non-pregnant women were selected as normal controls. Symptoms of depression were evaluated in the pregnant study subjects using the diagnostic criteria outlined in the Edinburgh Postnatal Depression Scale (EPDS). The pregnant subjects were divided into three groups perinatal non-depression controls (n=61), postpartum depression-recovery (n=18), and postpartum depression (n=25). Results : The plasma concentration of TGF-β1, IGF-1 was higher in the pregnant group than in non-pregnant controls (TGF-β1 ; p<0.01, IGF-1 ; p=0.026). At 24 weeks of pregnancy and 6 weeks of delivery, there were no significant differences in the plasma concentration of TGF-β1, IGF-1, β-NGF, IL-2, IL-4, IL-6, IFN-γ, TNF-α between the three groups. There was no statistically significant difference in all three groups during the course of depression in pregnant women. Conclusions : This study found significant difference in plasma cytokines concentrations between non-pregnant controls and perinatal non-depression controls.

Field Assessment of in Situ Remediation of NO3--contaminated Ground Water Using Zero-valent Iron/Bio Composite Media (영가철/바이오 복합처리제를 이용한 질산성 질소 오염 지하수의 현장 지중정화 적용성 평가)

  • Joo, Wan-Ho;Chang, Yoon-Young
    • Journal of Environmental Impact Assessment
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    • v.30 no.1
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    • pp.35-48
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    • 2021
  • In this study, the assessment of field applicability of in-situ remediation of nitrate-contaminated groundwater located in Yesan-gun was performed. Zero-valent iron/bio composite media injected PRB (Permeable Reactive Barrier) and monitoring well were installed in the contaminated groundwater site and monitored main remediation indicators during the PRB operation. Nitrate, nitrite, ammonia, Fe ion, TOC, and turbidity were analyzed and the diversity and population of microorganism in the PRB installed site were investigated for the verification of effect of injected PRB. In the study site where is an agricultural area, a river flows from west to east that forms a river boundary and the southern area has an impermeable sector. It was found that nitrate flows into the river, which is similar as groundwater flow. Simulation result for the fate of nitrate in groundwater showed steady state of nitrate arrived after 3~5 years passed. However, it is just to consider current conditions with no additional input of contaminant source, if additional input of contaminant source occurs contamination dispersion and time for steady state are expected to be increased. The monitoring results showed that Fe ion, TOC and turbidity in groundwater were not clearly changed in concentration after PRB installation, which indicates adaptability of the injected PRB for remediation of groundwater with no additional harmful effect to water quality. The concentration of nitrate maintained less than 5mg/L until 42 days after PRB installation and recovered its initial concentration after 84 days passed and showed termination of reactivity of injected zero-valent iron/bio composite media for removal nitrate. Nitrite and ammonia ions found after installation of PRB indicates reductive removal of nitrate. And the outstanding increase of microorganism diversity and population of Betaproteobacteria Class which includes denitrification microorganism explains biologically reductive removal of nitrate in injected PRB.

Development of A Material Flow Model for Predicting Nano-TiO2 Particles Removal Efficiency in a WWTP (하수처리장 내 나노 TiO2 입자 제거효율 예측을 위한 물질흐름모델 개발)

  • Ban, Min Jeong;Lee, Dong Hoon;Shin, Sangwook;Lee, Byung-Tae;Hwang, Yu Sik;Kim, Keugtae;Kang, Joo-Hyon
    • Journal of Wetlands Research
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    • v.24 no.4
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    • pp.345-353
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    • 2022
  • A wastewater treatment plant (WWTP) is a major gateway for the engineered nano-particles (ENPs) entering the water bodies. However existing studies have reported that many WWTPs exceed the No Observed Effective Concentration (NOEC) for ENPs in the effluent and thus they need to be designed or operated to more effectively control ENPs. Understanding and predicting ENPs behaviors in the unit and \the whole process of a WWTP should be the key first step to develop strategies for controlling ENPs using a WWTP. This study aims to provide a modeling tool for predicting behaviors and removal efficiencies of ENPs in a WWTP associated with process characteristics and major operating conditions. In the developed model, four unit processes for water treatment (primary clarifier, bioreactor, secondary clarifier, and tertiary treatment unit) were considered. Additionally the model simulates the sludge treatment system as a single process that integrates multiple unit processes including thickeners, digesters, and dewatering units. The simulated ENP was nano-sized TiO2, (nano-TiO2) assuming that its behavior in a WWTP is dominated by the attachment with suspendid solids (SS), while dissolution and transformation are insignificant. The attachment mechanism of nano-TiO2 to SS was incorporated into the model equations using the apparent solid-liquid partition coefficient (Kd) under the equilibrium assumption between solid and liquid phase, and a steady state condition of nano-TiO2 was assumed. Furthermore, an MS Excel-based user interface was developed to provide user-friendly environment for the nano-TiO2 removal efficiency calculations. Using the developed model, a preliminary simulation was conducted to examine how the solid retention time (SRT), a major operating variable affects the removal efficiency of nano-TiO2 particles in a WWTP.