• Proceedings of the Korean Society of Applied Pharmacology
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• 2001.11a
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• pp.99-99
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• 2001

The acid-labile subunit (ALS) associates with insulin-like growth factor (IGF)-I or -II and IGF binding protein-3 (IGFBP-3) to form a 150-kD complex in the circulation. This complex is thought to regulate the serum IGFs by restricting them in the vascular system and promotes their endocrine actions. Little is known about how ALS binds to IGFBP3, which connects the IGFs to ALS. Xenopus oocyte was utilized to study the function of ALS in assembling IGFs into the ternary complexes. Xenopus oocyte was shown to correctly translate in vitro transcribed mRNAs of ALS and IGFBP3. IGFBP3 and ALS mRNAs were injected in mixture and their products were immunoprecipitated by antisera against ALS and IGFBP3. Contrary to the traditional reports that ALS interacts only with IGF-bound IGFBP3, this study shows that ALS is capable of forming a binary complex with IGFBP3 in the absence of IGF. When cross-linked by disuccinimidyl substrate, band representing ALS-IGFBP3 complex was evident on the PAGE. IGFBP3 movement was monitored according to the distribution between the hemispheres. Following a localized translation in the vegetal hemisphere, IGFBP3 was shown to remain in the vegetal half in the presence of ALS. Different from wild type IGFBP3, however, mutant IGFBP3 freely diffused into the animal half despite the presence of ALS. Taken together, this study suggests that ALS may play an important role in sequestering IGFBP3 polypeptides via the intermolecular aggregation. Studies using this heterologous model will lead to a better understanding of the IGFBP3 and ALS assembling into the ternary structure and circulating IGF system.

• BMB Reports
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• v.35 no.2
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• pp.186-193
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• 2002

The acid-bible subunit (ALS) associates with the insulinlike growth factor (IGF)-I or II, and the IGF binding protein-3 (IGFBP-3) in order to form a 150-kD complex in the circulation. This complex may regulate the serum IGFs by restricting them in the vascular system and promoting their endocrine actions. Little is known about how ALS binds to IGFBP3, which connects the IGFs to ALS. Xenopus oocyte was utilized to study the function of ALS in assembling IGFs into the ternary complexes. Xenopus oocyte was shown to correctly translate in vitro transcribed mRNAs of ALS and IGFBP3. IGFBP3 and ALS mRNAs were injected in a mixture, and their products were immunoprecipitated by antisera against ALS and IGFBP3. Contrary to traditional reports that ALS interacts only with IGF-bound IGFBP3, this study shows that ALS is capable of forming a binary complex with IGFBP3 in the absence of IGF When cross-linked by disuccinimidyl suberate, the band that represents the ALS-IGFBP3 complex was evident on the PAGE. IGFBP3 movement was monitored according to the distribution between the hemispheres. Following a localized translation in the vegetal hemisphere, IGFBP3 remained in the vegetal half in the presence of ALS. However, the mutant IGFBP3 freely diffused into the animal half, despite the presence of ALS, which is different from the wild type IGFBP3. This study, therefore, suggests that ALS may play an important role in sequestering IGFBP3 polypeptides via the intermolecular aggregation. Studies using this heterologous model will lead to a better understanding of the IGFBP3 and ALS that assemble into the ternary structure and circulate the IGF system.

• Journal of Digital Convergence
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• v.13 no.12
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• pp.319-324
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• 2015

This paper proposes an integrated management system for products of game software development. Those products generated over software life-cycle are generally huge in numbers, various in kinds and interconnected in relationships among them. Especially they are highly complex and tightly coupled each other because they are used to build another world which is composed of multi-modal data such as graphics, sound, logics and so on. Therefore it is critical to manage development products in an integrated manner to guarantee the quality of software. The proposed system is designed to provide project/requirement/configuration/traceability management capabilities and interfacing to CASE standards. To achieve the objective, it is based on data warehouse technologies which provide central repositories of heterogeneous data, multi-aspect reporting&analysis capabilities, traceability, verification, etc.

• Proceedings of the Korea Institute of Fire Science and Engineering Conference
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• 1997.11a
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• pp.507-517
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• 1997

As structures are higher, large-sized and more complex, we should detect the fire at the beginning and cope with it to reduce the loss of mankind and the physical damage due to fire. So we have investigated and developed various kinds of fire detection system, and do the efforts for minimizing the nonfire alarm. As there exists a close relationship between the technology development and the market potential, a comparison between the number of fires in special buildings and detection types were made to find out market potential based on the annual statistics on fire products inspection. In addition, we have discussed the causes of nonfire alarm and the fire detection system and prospect the research trend of the fire detection system.

• Journal of KIISE:Computing Practices and Letters
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• v.11 no.5
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• pp.458-463
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• 2005

The total asset management system is used for banks or financial institutions for the management of trusteed assets or own assets and it is divided into three systems: the front-office system, the middle-office system and the back-office system by its business areas and functionalities. This kind of asset management system is a huge and complex system handling large data and various financial products, and requires professional knowledges like accounting, financial product specific knowledge, compliance and regulations, etc. It also performs high level computation for NAV calculation and risk measurement on every day Therefore, it needs absolute stability, extendability and efficiency and should handle the frequent change of regulation and products and connectivity with outdoor institutions. In this paper, we report our successful development of such a system and discuss issues regarding its efficient system design and system construction.

• Proceedings of the Korea Society for Energy Engineering kosee Conference
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• 1998.05a
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• pp.235-242
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• 1998

Aiming at one of decisive alternatives for long term aspect of nuclear power concerns, an integral and closed nuclear system, AMBIDEXTER (Advanced Molten-salt Break-even Inherently-safe Dual-mission Experimental and TEst Reactor) concept is under development. The AMBIDEXTER complex essentially comprises two mutually independent loops of the radiation/material transport and the heat/energy conversion, centered at the integrated reactor assembly, which enables one to utilize maximum benefits of nuclear energy under minimum risks of nuclear radiation. And it provides precious radioisotopes and radiation sources from its waste stream. Also the reactor operates at very low level of fission products inventory throughout its lifetime. The nuclear and thermalhydraulic characteristics of the molten TH/$^{233}$ U fuel salt extend the capability of the self-sustaining AMBIDEXTER fuel cycle to enhance resource security and safeguard transparency. The reactor system is consisted of a single component module of the core, heat exchangers and recirculation pumps with neither pipe connections nor active valves in between, which will significantly improve inherent features of nuclear safety. States of the core technologies associated with designing and developing the AMBIDEXTER concept are mostly available in commercialized form and thus demonstration of integral aspects of the concept should be the prime area in future R＆D programs.

• Archives of Pharmacal Research
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• v.27 no.6
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• pp.619-623
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• 2004

Tissue factor (TF, tissue thromboplastin) is a membrane bound glycoprotein, which acceler-ates the blood clotting, activating both the intrinsic and the extrinsic pathways to serve as a cofactor for activated factor VII (Vila). The TF-factor Vila complex (TF/VIIa) proteolytically activates factors IX and X, which leads to the generation of thrombin and fibrin clots. In order to isolate TF inhibitors, by means of a bioassay-directed chromatographic separation technique, from the leaves of Eriobotrya japonica Lindley (Rosaceae), a known sesquiterpene glycoside (2) and ferulic acid (3) were isolated as inhibitors that were evaluated using a single-clotting assay method for determining TF activity. Another sesquiterpene glycoside (1) was also isolated but was inactive in the assay system. Compound 3 was yielded by alkaline hydrolysis of compound 2. The structures of compounds 1, 2, and 3 were identified by means of spectral analysis as $3-O-{\alph}-L-rhamnopyranosyl-(1{\rightarrow}4)-a-L-rhamnopyranosyl-(1{\rightarrow}2)-[{\alph}-L-rhamnopyrano-syl-(1{\rightarrow}6)]-{\beta}-D-glucopyranosyl nerolidol$ (1), $3-O-{\alph}-L-rhamnopyranosyl-(1{\rightarrow}4)-{\alph}-L-rhamnopyr-anosyl-(1{\rightarrow}2)-[{\alph}-L-(4-trans-feruloyl)-rhamnopyranosyl-(1{\rightarrow}6)]-{\beta}-D-glucopyranosyl$ nerolidol (2) and ferulic acid (3), respectively. Compounds 2 and 3 inhibited 50% of the TF activity at con-centrations of 2 and $369{\;}\mu\textrm{m}/TF$ units, respectively.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.13 no.8
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• pp.4212-4226
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• 2019

In this paper, a design and implementation for direct deal distribution platform is proposed to bypass the complex traditional distribution structure of agricultural market, as one of the fields where distribution patterns have changed. In the case of domestic agricultural distribution, demand and supply are unstable since the sales market is excessively concentrated in the designated wholesale market. Besides sales must go through multiple stages of distribution leading to problems in freshness and stability of agricultural products and downward pressure on profit margins for producers. To solve the above mentioned issues, we propose a cloud service convergence direct deal distribution platform based on asynchronous-driven Node.js. The proposed platform can facilitate a variety of direct trading functions and also access to visualization information related to agricultural products, which may increase user confidence at an intermediary-free direct transactions platform. First, we describe the requirements of intermediary-free direct transactions of agricultural products and transaction entities. Next the database structure and transaction functions are designed and then implemented according to those requirements. Finally, an API based cloud convergence service structure is designed to provide the analyzed information to ensure a trustworthy system.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2003.06a
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• pp.158-161
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• 2003

Recently, the evolution in production techniques (e.g. high-speed milling), the complex shapes involved in modem production design, and the ever increasing pressure for higher productivity demand a drastic improvement of the dynamic behavior of the machine tool axes used in production machinery. And also machine tools of multi functional and minimized parts are increasingly required as demand of higher accurate in some fields such as electronic and optical components etc. The accuracy and the productivity of machined parts are natural to depend on the linear system of machine tools. The complex workpiece surfaces encountered in present-day products and generated by CAD systems are to be transformed into tool paths for machine tools. The more complex these tool paths and the higher the speed requirements, the higher the acceleration requirements are needed to the machine tool axes and the motion control system, and the more difficult it is to meet the requirements. The traditional indirect drive design for high speed machine tools, which consists of a rotary motor with a ball-screw transmission to the slide, is limited in speed, acceleration, and accuracy. The direct drive design of machine tool axes. which is based on linear motors and which recently appeared on the market. is a viable candidate to meet the ever increasing demands, because of these advantages such as no backlash, less friction, no mechanical limitations on acceleration and velocity and mechanical simplicity. Therefore performance tests were carried out to machine tool axes based on linear motor. Especially, dynamic characteristics were investigated through circular test.

• The Korean Journal of Physiology and Pharmacology
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• v.2 no.6
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• pp.779-786
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• 1998

A reversed-phase high-performance liquid chromatogrphy (RP-HPLC) has been developed to analyze the metabolites of arachidonic acid based on the specificities of ultraviolet absorption of these various metabolites and is sensitive to the nanogram level. This procedure makes it possible to extract complex mixtures of eicosanoids efficiently with a single step and to analyze them simultaneously by RP-HPLC from biological samples using octadesylsilyl silica extraction column and $PGB_2$ as an internal standard. The cyclooxygenase products {prostaglandin $(PG)D_2,\;PGE_1,\;PGE_2,\;PGF_{1{\alpha}},\;PGF{2{\alpha}},\;6-keto-PGF_{1{\alpha}},$ and thromboxane $B_2(TXB_2)}$ and lipid peroxidation product, isoprostanes, of arachidonic acid were monitored by one isocratic HPLC system at 195 nm wavelength. The lipoxygenase products ${leukotriene(LT)B_4,\;LTC_4,\;LTD_4,$ and 5-hydroxyeicosatetraenoic acid (5-HETE), 12-HETE, 15-HETE} were measured by another isocratic HPLC system at 280 nm for LTs and 235 nm for HETEs. This method provides a simple and reliable way to extract and assess quantitatively the final arachidonic acid metabolites.