• Bulletin of the Korean Chemical Society
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• v.21 no.10
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• pp.1052-1054
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• 2000

Binding geometries of $[Ru(II)(110-phenanthroline)_2L]^2+$, complexes (where L = dipyrido [3,2-a:2',3'-c]phena-zine (DPPZ) or benzodipyrido[3,2-a:2',3'-c] phenazine (BDPPZ)) to poly(dG)${\cdot}$poly(dC)${\cdot}$poly(dC) + triplex DNA (CGC + triplex) has been investigated by linear dichroism and normal absorption spectroscopy. Analysis of the linear dichroism for the CGC+ triplex and $[Ru(II)(phen)_2BDPPZ]^2+$ complex indicates that the extended ligand of the metal complex lie perpendicular to the polynucleotide helix axis. Together with strong hypochromism and red shift in the interligand absorption region, we concluded that the extended BDPPZ or DPPZ ligand in-tercalated between the bases of polynucleotide. The spectral properties of the metal complexes bound to CGC+ triplex are similar to those bound to $poly(dA)[poly(dT)]^2$ triplex (Choi et al., Biochemistry 1997, 36, 214), sug-gesting that the metal complex is located in the minor groove of the CGC+ triplex.

• Analytical Science and Technology
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• v.32 no.2
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• pp.35-47
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• 2019

In this study, high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was employed to detect 26 antidiabetic compounds in adulterated dietary supplements using a simple, selective method. The work presented herein may help prevent incidents related to food adulteration and restrict the illegal food market. The best separation was obtained on a Shiseido Capcell Pak(R) C18 MG-II ($2.0mm{\times}100mm$, $3{\mu}m$), which improved the peak shape and MS detection sensitivity of the target compounds. A gradient elution system composed of 0.1 % (v/v) formic acid in distilled water and methanol at a flow rate of 0.3 mL/min for 18 min was utilized. A triple quadrupole mass spectrometer with an electrospray ionization source operated in the positive or negative mode was employed as the detector. The developed method was validated as follows: specificity was confirmed in the multiple reaction monitoring mode using the precursor and product ion pairs. For solid samples, LOD ranged from 0.16 to 20.00 ng/mL and LOQ ranged from 0.50 to 60.00 ng/mL, and for liquid samples, LOD ranged from 0.16 to 20.00 ng/mL and LOQ ranged from 0.50 to 60.00 ng/mL. Satisfactory linearity was obtained from calibration curves, with $R^2$ > 0.99. Both intra and inter-day precision were less than 13.19 %. Accuracies ranged from 80.69 to 118.81 % (intra/inter-day), with a stability of less than 14.88 %. Mean recovery was found to be 80.6-119.0 % and less than 13.4 % RSD. Using the validated method, glibenclamide and pioglitazone were simultaneously determined in one capsule at concentrations of 1.52 and 0.53 mg (per capsule), respectively.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.20 no.8
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• pp.791-796
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• 2009

A hybrid iterative physical optics-method of moments(IPO-MoM) technique is presented for the analysis of jet engine structures which are both electrically large and complex. In this technique, the IPO method is used to analyze smooth inlet region and the MoM method is used to analyze electrically complex region inclusive of blades and hub. It is efficient and accurate by virtue of combining the respective merits of both methods. Numerical results are presented and validated through comparison with Mode-FDTD and measured results.

• Bulletin of the Korean Chemical Society
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• v.23 no.2
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• pp.253-261
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• 2002

Vibrational properties of ferrocyanide complex ion, $[Fe(CN)_6]^{4-}$ , have been studied based on the force constants obtained from the density functional calculations at B3LYP/$6-31G^{\ast\ast}$ level by means of the normal mode analysis using new bond angle and linear angle internal coordinates recently developed. Vibrations of ferrocyanide were manipulated by twenty-three symmetry force constants. The angled bending deformations of C-Fe-C, the linear bending deformations of Fe-C${\equiv}$N and the stretching vibrations of Fe-C have been quantitatively assigned to the calculated frequencies. The force constants in the internal coordinates employed in the modified Urey-Bradley type potential were evaluated on the density functional force field applied, and better interaction force constants in the internal coordinates have been proposed. The valence force constants in the general quadratic valence force field were also given. The stretch-stretch interaction and stretch-bending interaction constants are not sensitive to the geometrical displacement in the valence force field.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2005.05a
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• pp.236-240
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• 2005

This paper deals with friction-induced vibration of disc brake system under constact friction coefficient. A linear, finite element model to represent the floating caliper disc brake system is proposed. The complex eigenvalues are used to investigate the dynamic stability and in order to verify simulations which are based on the FEM model, The comparison of experimental and analytical results shows a good agreement and the analysis indicates that mode coupling due to friction force and geometric instability is responsible fur disc brake squeal. And the Front brake system reduced the squeal noise using design of experiment method(DOE). This helped to validate the FEM model and establish confidence in the simulation results. Also they may be useful during real disk brake model.

• YAKHAK HOEJI
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• v.49 no.5
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• pp.428-436
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• 2005

DNA topoisomerase I(TOP1) helps the control of DNA replication, transcription and recombination by assist­ing breaking and rejoining of DNA double strand. Camptothecin (CPT) and its derivative, topotecan, are known to inhibit TOP1 by intercalating into TOP1-DNA complex. Recently various non-CPT intercalators are synthesized for a new class of TOP1 inhibitors. In this study, six compounds isolated from Poria cocos were investigated for their interaction with TOP1­DNA complex using the flexible docking program, FlexiDock. The binding modes were analyzed and compared with the TOP1 inhibition activities. The compounds that showed potent activity were intercalated between the + 1/-1 base pairs of DNA, located near the active site phosphotyrosine723 and formed hydrogen bonds with active site residues. On the other hand, compounds with no activity were not docked at all. The binding modes were well correlated with the inhibition activity, suggesting the possibility that potent inhibitors can be designed from the information presented by the docking study.

• Journal of the Korean Society of Safety
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• v.12 no.4
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• pp.27-38
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• 1997

The effect of geometry factors on the combined mode stress intensity factor behaviors of a slant crack in a non-uniform thickness material was analysed by 2-dimensional theoretical analysis. The analysis is based on the Laurent's series expansions of complex potentials where the complex coefficients of the series are determined from the compatibility and the equilibrium conditions of the thickness interface and the stress free conditions of the crack surface. In numerical calculations the perturbation technique is employed. The expressions for the crack tip stress intensity factor are given in the form of power series of dimensionless crack length $\lamda$, and the function of crack slant angle $\alpha$ and thickness ratio $\beta$. The results of numerical calculations for each problems are represented as the correction factors F($\lamda$, $\alpha$, $\beta$). The results clearly show the following characteristics : The correction factors of the combined mode stress intensity factors for a non-uniform thickness material can be defined in the form of F($\lamda$, $\alpha$, $\beta$). The stress intensity factor values for a given crack length are decreased with increase of thickness ratio $\beta$.

• Steel and Composite Structures
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• v.22 no.5
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• pp.1039-1054
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• 2016

This paper presents the dynamic characteristics analysis of the purlin-sheet roofs by the random vibration theories. Results show that the natural vibration frequency of the purlin-sheet roof is low, while the frequencies and mode distributions are very intensive. The random vibration theory should be used for the dynamic characteristics of the roof structures due to complex vibration response. Among the first 20th vibration modes, the first vibration mode is mainly the deformations of purlins, while the rest modes are the overall deformations of the roof. In the following 30th modes, it mainly performs unilateral local deformations of the roof. The frequency distribution of the first 20th modes varies significantly while those of the following 30th modes are relatively sensitive. For different parts, the contributions of vibration modes on the vibration response are different. For the part far from the roof ridge, only considering the first 5th modes can reflect the wind-induced vibration response. For the part near the ridge, at least the first 12 modes should be considered, due to complex vibration response. The wind vibration coefficients of the upwind side are slightly higher than that of the leeward side. Finally, the corresponding wind vibration coefficient for the purlin-sheet roof is proposed.

• Structural Engineering and Mechanics
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• v.64 no.4
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• pp.473-485
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• 2017

An isolated building, composed of superstructure and isolation system which have very different damping properties, is typically non-classical damping system. This results in inapplicability of traditional response spectrum method for isolated buildings. A multidimensional response spectrum method based on complex mode superposition is herein introduced, which properly takes into account the non-classical damping feature in the structure and a new method is developed to estimate velocity spectra from the commonly used displacement or pseudo-acceleration spectra based on random vibration theory. The error of forced decoupling method, an approximated approach, is discussed in the viewpoint of energy transfer. From the base-isolated benchmark model, as a numerical example, application of the procedure is illustrated companying with comparison study of time-history method, forced decoupling method and the proposed method. The results show that the proposed method is valid, while forced decoupling approach can't reflect the characteristics of isolated buildings and may lead to insecurity of structures.

• Bulletin of the Korean Chemical Society
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• v.28 no.6
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• pp.959-964
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• 2007

The benzene complexes with dimethyl sulfur (DMS) and fluorinated DMS (FDMS) have been investigated using ab initio calculations. The natural bond orbital (NBO) charge population on S atom varies remarkably for different conformations of DMS and FDMS, which determines the possible binding modes for their benzene complexes. The electronegative substituent at the methyl group of DMS causes a significant change in the molecular electrostatic potential around the sulfur atom and changes the interaction mode with aromatic ring. It was found that the sulfur…π interaction mode does not occur in the DMS-benzene complex, while it does in the FDMS-benzene complex. Both B3LYP and MP2 methods provide reliable structures, while the interaction energy obtained by B3LYP is unreliable.