• The Plant Pathology Journal
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• 제32권4호
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• pp.281-289
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• 2016

The transcription cofactor Swi6 plays important roles in regulating vegetative growth and meiosis in Saccharomyces cerevisiae. Functions of Swi6 ortholog were also characterized in Fusarium graminearum which is one of the devastating plant pathogenic fungi. Here, we report possible role of FgSwi6 in the interaction between F. graminearum and Fusarium graminearum virus 1 (FgV1) strain DK21. FgV1 perturbs biological characteristics of host fungi such as vegetative growth, sporulation, pigmentation, and reduction of the virulence (hypovirulence) of its fungal host. To characterize function(s) of FgSWI6 gene during FgV1 infection, targeted deletion, over-expression, and complementation mutants were generated and further infected successfully with FgV1. Deletion of FgSwi6 led to severe reduction of vegetative growth even aerial mycelia while over-expression did not affect any remarkable alteration of phenotype in virus-free isolates. Virus-infected (VI) FgSWI6 deletion isolate exhibited completely delayed vegetative growth. However, VI FgSWI6 over-expression mutant grew faster than any other VI isolates. To verify whether these different growth patterns in VI isolates, viral RNA quantification was carried out using qRT-PCR. Surprisingly, viral RNA accumulations in VI isolates were similar regardless of introduced mutations. These results provide evidence that FgSWI6 might play important role(s) in FgV1 induced phenotype alteration such as delayed vegetative growth.

• Molecular & Cellular Toxicology
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• 제2권3호
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• pp.159-165
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• 2006

To determine the anticancer effect of D-amygdalin (D-mandelinitrole-${\beta}$-D-gentiobioside) in human chronic myeloid leukemia cells K562, we profiled the gene expression between amygdalin treatment and control groups. Through 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay, the cytotoxicity of D-amygdalin was $57.79{\pm}1.83%$ at the concentration of 5 mg/mL for 24 h. We performed cDNA microarray analysis and compared the gene expression profiles between D-amygdalin (5 mg/mL, 24 h) treatment and control groups. Among the genes changed by D-amygdalin, we paid attention to cell cycle-related genes, and particularly cell cycle regulator genes; because arrest of cell cycle processing was ideal tactic in remedy for cancer. In our data, expressions of cyclin-dependent kinase inhibitor 1B (p27, Kip1) (CDKN1B), ataxia telangiectasia mutated (includes complementation groups A, C, and D) (ATM), cyclin-dependent kinase inhibitor 1C (p57, Kip2) (CDKN1C), and CHK1 checkpoint homolog (CHEK1, formally known as CHK1) were increased, while expressions of cyclin-dependent kinase 2 (CDK2), cell division cycle 25A (CDC25A), and cyclin E1 (CCNE1) were decreased. The pattern of these gene expressions were confirmed through RT-PCR. Our results showed that D-amygdalin might control cell cycle regulator genes and arrest S phase of cell cycle in K562 cells as the useful anticancer drug.

• 미생물학회지
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• 제37권1호
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• pp.42-48
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• 2001

출아 효모인 Saccharomyces cerevisiae의 베타-1,3-글루칸 생합성의 결함을 초래하는 돌연변이 유전자(sool-1)를 분리하여 돌연변이 부위의 염기서열을 결정하고 그 특성을 분석하였다. cool-1 유전자의 염기서열 분석 결과, 681번의 염기인 G가 A로 치환되어 Soolp의 $Gly^{227}$이 Asp로 치환되는 결과를 나타내는 것으로 판명되었고, cool-1 유전자는 기존에 보고된 retl-1 유전자와 동일한 돌연변이 유전자로 판명되었다. 그러나, ret1-1이 나타내는 온도감수성 형질은 배지에 1.2 M sorbitol 등의 삼투안정제를 첨가하면 극복될 수 있으며, cool-l/retl-1의 돌연변이 부위가 세포벽합성 관련 단백질의 번역 후 수식과정에 영향을 미친 것임을 확인하였다. 한편, Soolp/$\alpha$-COP의 N-말단에 존재하는 6개의 WD40 domain중 5번째 WD40 domain이 효모의 세포벽 합성이나 구조유지에 중요한 역할을 담당할 것임을 시사하는 결과를 얻었다.

• 미생물학회지
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• 제36권1호
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• pp.46-51
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• 2000

장티푸스는 Salmonella typhi에 의해 유발되는 장감염성 질환으로 사람과 동물에 공통되는 질병이다. 본 연구에서는 국립보건원과 공동연구를 수행하여 한국형 장티푸스 유발균인 S. typhi KNIH100을 분리하였다. 분리된 S. typhi KNIH100의 염색체 DNA로부터 방향족 아미노산의 생합성에 관여하는 효소인 5-enolpyruvylshikimate-3-phosphate synthetase를 암호화하는 aroA 유전자를 포함하는 약 5.0 kb의 SalI절편을 pBluescriptII SK(+) vector와 aroA 돌연변이주인 E. coli CGSC2829를 이용하여 클로닝하였다. 그리고 이 클론을 pSAL80이라 명명하였다. 클로닝된 재조합 plasmid인 pSAL80에는 ATG 개시코돈과 TGA 종결코돈을 포함하는 1,284 염기로 구성된 aroA 유전자가 위치하고 있었으며, 다른 장내세균과 마찬가지로 serC와 하나의 오페론을 구성하고 있음을 밝혔다. 또한 S. typhi Ty2, S. typhimurium, 그리고 E. coli 등 다른 장내세균의 aroA 유전자와 상동성을 비교하여 본 결과 각각 99%, 95%, 77%의 상동성을 나타내었다.

• 한국식생활문화학회지
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• 제28권5호
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• pp.480-487
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• 2013

This study is conducted to investigate to the consumption pattern of Kimchi and perception about the functional Kimchi of consumer. The survey was done between October 1 to October 15, 2011 among 294 male and female adults aged 19 and over in Seoul and Gyeonggi-do areas. The gender distribution of subjects was 33.3% males and 66.7% females. 64.3% of subjects prepared Kimchi by themselves, 23.5% of subjects received Kimchi from relatives and 12.2% of subjects purchased Kimchi from the market. In addition, the rate of preparing Kimchi at home is highest in those aged fifty or over. Only 41.8% of subjects knew how to make Kimchi. 72.1% of subjects responded that they ate Kimchi one or more a day. 46.6% of subjects have purchased commercial Chinese cabbage Kimchi. The amount of one-time purchase of commercial Kimchi were investigated; 45.2% of subjects have been buying 500-1 kg, 34.4% of the subjects bought less than 500 g, and 11.2% of subjects bought 1-3 kg. 28.2% of subjects buy Kimchi at the supermarket and warehouse market. With regard to the evaluation of Kimchi taste, most consumers were not satisfied with the sweetness of Kimchi. In this result, the perception about functional Kimchi was very low. Consumer's demands were as follows: nutrient enhancement, strengthening of biologically active substances, lactic acid bacteria enhancement in order. Small sales units were preferred by the consumers, and complementation of sweetness of kimchi was required. Various Kimchi including functional Kimchi must be developed to meet the needs of consumers.

• 한국측량학회:학술대회논문집
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• 한국측량학회 2004년도 추계학술발표회 논문집
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• pp.405-410
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• 2004

In mid-90's, the Korean government introduced the GIS(Geographic Information System) to digitalize every topography of national land and thereby, index locations and attributes of various urban facilities to construct a system whereby every information could be managed and operated in an integrated way, but the reliability of such geographic information has yet to be tested, much less its modification, complementation and maintenance. Under such circumstances, this study was aimed at constructing a reference point infrastructure for Seoul and Kyonggi area and comparing the data obtained from the GPS operation and various facility location data with the existing GIS data to address the problems of GIS operation and suggest their solutions. As a result of calculating the GPS reference point data and analyzing the deviations of the unknown point data in comparison with the fixed point ones, it was found that the horizontal location values were reliable within +/- 5cm, but that the above-sea level values varied as much as 1.4m depending on the deployment of pre-set fixed points determined by the direct level gauging. In addition, as a result of directly surveying major facilities around the roads based on the coordinates of the urban reference points networked with such a reference point system to check their conformity to existing data, it was confirmed that the difference was as wide as 2m. Such differences may be attributable to the fact that the data with their geographic information not confirmed are used as basic data for GIS. Hence, this study suggests the ways to set the absolute geographic data based on reference points and test the reliability of existing data and thereby, suggests a methods to solve the problems.

• 한국균학회소식:학술대회논문집
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• 한국균학회 2015년도 추계학술대회 및 정기총회
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• pp.28-28
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• 2015

Asexual reproduction or conidiation in aspergilli is a primary mean to produce their progenies that is environmentally and genetically controlled tightly. Previously, intensive researches in the model fungus Aspergillus nidulans disclosed some genes playing important roles in asexual and sexual development. Among them, one gene encoding a putative helix-loop-helix (HLH) transcription factor, named ndrA, has been isolated and characterized as a downstream regulator of developmental master regulator NsdD. By using comparative genome search of A. niduans NdrA protein, its orthologues have been identified in A. fumigatus and A. flavus, respectively (AfudrnA and AfldrnA). Deletion of the ndrA genes in both Aspergillus species made them unable to produce the conidia yet abundant production of sclerotia in A. flavus. Complementation of ndrA deletion strains by intact ndrA ORFs has restored the conidiation as in the control strains. In A. fumigatus, ndrA deletion also resulted in loss of conidiation phenotype. Northern analyses showed that the ndrA genes in both Aspergillus species are highly expressed at the early stage of the conidiation. Interestingly, the ndrA genes were found to be necessary for the proper expression of brlA genes. Antifungal sensitivity test revealed that the ndrA genes might be responsible for the sensitivity or resistance to some antifungal agents. However, ndrA deletion did not greatly influence the growth in both strains. And the A. flavus ndrA gene did not affect the aflatoxin production. Taken together, ndrA genes in Aspergillus species could be an important positive regulator of conidiation under the regulation of the nsdD gene yet upstream of the brlA gene.

• 재활복지공학회논문지
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• 제11권2호
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• pp.187-198
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• 2017

이 연구에서는 우리나라의 모바일 앱 접근성 KS, 미국의 재활법 508조 개정 표준 및 유럽의 BBC 지침을 기준으로 개방형 플랫폼인 안드로이드 모바일 뱅킹 앱의 접근성을 분석하였다. 실험 결과, 우리나라 8개 시중은행의 모바일 뱅킹 앱 중 상기 3가지 표준의 세부 항목을 모두 만족하는 앱은 한 곳도 없었다. 대표적인 위반 항목은 대체 텍스트 미비, 키보드 초점 위반, 컨트롤 크기 및 간격 미준수, 명도대비 비율 낮음 등이었다. 이들 위반사례는 시각장애인, 상지장애인 및 저시력인과 고령자의 접근을 불가능하게 만든다는 점에서 치명적인 오류이다. 모바일 뱅킹 앱이 접근성을 준수하지 않는 원인에 있어서는 모바일 앱 개발자와 제공자들의 접근성에 관한 인식이 낮고 접근성 구현 방법을 제대로 숙지하지 못한 것으로 분석되었다. 우리나라의 모바일 앱 접근성 지침을 재활법 508조 개정 표준 및 BBC 표준과 비교한 결과, 많은 항목이 누락되어 있었다. 또한 평가 기준이 모호하고 추상적이어서 개발자들이 구체적으로 참고하기 어려운 측면이 있는 것으로 판단되었다. 따라서 모바일 앱 접근성 향상을 위한 개발자 및 정부의 노력과 더불어 표준의 보완이 필요하다.

• 생명과학회지
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• 제17권1호
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• pp.39-44
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• 2007

분열형 효모에서 Rqh1은 Top3과 함께 vegetative growth에 필수적이다. $rqh^-$ 돌연변이는 DNA damaging agent에 민감성을 보이는데 이때, 부적절한 유전자 발현, 세포 신장, 염색체의 불안전성, 비정상적인 다중격막, 발아의 결핍을 포함한 넓은 범위의 표현형을 보인다. rqh1-overexpression cell 역시 rqh1 deletion mutant에서 보이는 DNA damaging agent 민감성을 관찰할 수 있다. 논문은 nmtl promoter를 가지는 PREP vector에 Rqhl이 과발현 할 때 나타나는 DNA damaging agent 민감성를 보상하는 유전자를 찾아 $rqh1^+$의 기능을 알아보는 것이다. 여기서 보상능이 보이는 rqh156, rqh172 두 개의 돌연변이를 골라냈다. rqhl deletion mutant의 DNA damaging agent 민감성은 rqh156, rqh172의 발현에 의해 보상 되어지는 것을 확인하였다.

• 한국콘텐츠학회논문지
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• 제13권7호
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• pp.24-37
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• 2013

본 연구는, 픽사의 와 에 나타난 부모와 자녀 캐릭터 사이의 갈등구도를, MBTI의 유형역동이라는 관점에서 비교 분석하고자 했다. 이에, 캐릭터의 성격유형과 심리기능의 위계를 추론하고, 유형역동에 의한 갈등과 화해의 구조를 분석하였다. 작품 분석을 통해 도출된 결론은: (1) 부모의 사고기능이 자녀의 직관기능을 구속하는 구조로, '현실과 가능성의 충돌'로 은유됨. (2) 보완 역할 캐릭터의 외향적 심리기능이, 결핍 캐릭터의 미발달된 3차기능을 보완하면서, 유형발달에 기여함. (3) 에서는, 갈등주체에 의한 직접적 단방향의 보완을 이루며, (4) 에서는, 갈등주체가 아닌 거울캐릭터에 의한 간접적 쌍방향의 상호보완을 이루는 방법론의 차이를 보인다.