• International Journal of Fuzzy Logic and Intelligent Systems
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• 제5권4호
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• pp.297-301
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• 2005

In this paper, we propose a method to estimate camera motion parameter based on invariant point features. Typically, feature information of image has drawbacks, it is variable to camera viewpoint, and therefore information quantity increases after time. The LM(Levenberg-Marquardt) method using nonlinear minimum square evaluation for camera extrinsic parameter estimation also has a weak point, which has different iteration number for approaching the minimal point according to the initial values and convergence time increases if the process run into a local minimum. In order to complement these shortfalls, we, first propose constructing feature models using invariant vector of geometry. Secondly, we propose a two-stage calculation method to improve accuracy and convergence by using homography and LM method. In the experiment, we compare and analyze the proposed method with existing method to demonstrate the superiority of the proposed algorithms.

• 한국수정란이식학회지
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• 제28권3호
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• pp.289-295
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• 2013

Xenotransplantation of pig organs into primates results in fatal damage, referred as hyperacute rejection (HAR), and acute humoral xenograft rejection (AHXR), to the organ graft mediated by antibodies pre-existing and newly-producing in primates against their cognate pig antigens. Functional ablation of ${\alpha}1$,3-galactosyltransferase (Gal-T KO) of pig which is an enzyme involved in synthesis of Gala1-3Galb1-4GlcNAc-R antigen is essentially required to prevent HAR. Moreover, additional genetic modification under Gal-T KO background for enforced expression of human complement regulatory proteins which can inhibits complement activation is known to effectively imped HAR and AHXR. In this study, we constructed a membrane cofactor protein (MCP) expression cassette under control of human $EF1{\alpha}$ promoter. This cassette was inserted between homologous recombination regions corresponding to Gal-T locus. Subsequently this vector was introduced into ear skin fibroblasts of female pig by nucleofection. We were able to obtained 40 clones by neomycin selection and 4 clones among them were identified as clones targeted into Gal-T locus of MCP expression cassette by long-range PCR. Real time RT-PCR was shown to down-regulation of Gal-T expression. From these results, we demonstrated human $EF1{\alpha}$ promoter could induce efficient expression of MCP on cell surface of fibroblasts of female pig.

• IMMUNE NETWORK
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• 제12권1호
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• pp.33-39
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• 2012

Background: Therapeutic approaches using monoclonal antibodies (mAbs) against complement regulatory proteins (CRPs:i.e.,CD46,CD55 and CD59) have been reported for adjuvant cancer therapy. In this study, we generated a recombinant 1E8 single-chain anti-CD59 antibody (scFv-Fc) and tested anti-cancer effect.by using complement dependent cytotoxicity (CDC). Methods: We isolated mRNA from 1E8 hybridoma cells and amplified the variable regions of the heavy chain (VH) and light chain (VL) genes using reversetranscriptase polymerase chain reaction (RT-PCR). Using a linker, the amplified sequences for the heavy and light chains were each connected to the sequence for a single polypeptide chain that was designed to be expressed. The VL and VH fragments were cloned into the pOptiVEC-TOPO vector that contained the human CH2-CH3 fragment. Then, 293T cells were transfected with the 1E8 single-chain Fv-Fc (scFv-Fc) constructs. CD59 expression was evaluated in the prostate cancer cell lines using flow cytometry. The enhancement of CDC effect by mouse 1E8 and 1E8 scFv-Fc were evaluated using a cytotoxicity assay. Results: The scFv-Fc constructs were expressed by the transfected 293T cells and secreted into the culture medium. The immunoreactivity of the secreted scFv-Fc construct was similar to that of the mouse 1E8 for CCRF-CEM cells. The molecular masses of 1E8 scFv-Fc were about 120 kDa and 55 kDa under reducing and non-reducing conditions, respectively. The DNA sequence of 1E8 scFv-Fc was obtained and presented. CD59 was highly expressed by the prostate cancer cell line. The recombinant 1E8 scFv-Fc mAb revealed significantly enhanced CDC effect similar with mouse 1E8 for prostate cancer cells. Conclusion: A 1E8 scFv-Fc construct for adjuvant cancer therapy was developed.

• 인터넷정보학회논문지
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• 제6권6호
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• pp.187-197
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• 2005

본 논문에서는 불변 점 특징에 기반한 카메라 동작인수 측정방법을 제안한다. 일반적으로 영상의 특징정보는 카메라 뷰포인트에 따라 변하는 단점이 있어 시간이 지나면 정보량이 증가하게 된다. 또한 카메라 외부인수 산출을 위한 비선형 최소제곱 측정을 이용한 LM 방법은 초기값에 따라 최소점에 근접하는 반복회수가 다르고 지역 최소점에 빠질 경우 수렴시간이 증가하는 단점이 있다. 본 논문에서는 이러한 문제를 개선하기 위해 첫째, 기하학의 불변 벡터를 사용하여 특징 모델을 구성하는 것을 제안하였다. 둘째, 2D 호모그래피와 LM 방법을 이용하여 정확도와 수렴도를 향상시키는 2단계 측정 방법을 제안하였다. 실험에서는 제안한 알고리즘의 우수성을 입증하기 위해 기존방법과 제안한 방법을 비교 분석하였다.

• 한국콘텐츠학회논문지
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• 제10권4호
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• pp.19-27
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• 2010

최근에 고속 genome-wide RNA 간섭 스크리닝 기술은 복잡한 세포 기능을 이해하는 생명공학 연구의 핵심적인 도구로 자리 잡고 있다. 그러나 관련 연구에서 발생되는 수많은 영상을 수작업을 통해 분석하는 것은 많은 시간과 노력이 요구된다. 따라서 세포영상의 자동분석 기술은 매우 시급히 확보되어야 하는 기술이며, 그 중 영상 분할은 자동분석을 위한 첫 단계로서 가장 중요한 과정이라 할 수 있다. 세포영상의 자동분할에서는 영역의 겹침 현상과 영역별 모양의 다양성 및 영상 특성의 불균일성 등이 정확한 세포 분할을 어렵게 만드는 주원인으로 작용한다. 본 논문에서는 이러한 문제점을 극복하기 위해 영상 특징들의 국부적인 연속성과 특징 벡터 기반의 워터쉐드 알고리즘을 적용한 새로운 자동 세포 분할 알고리즘을 제안한다. 영상 특징들의 연속성을 국부적인 영역으로 제한함으로써 영역별 모양의 다양성 및 영상 특성의 불균일성에 따른 문제점을 극복할 수 있으며, 특징벡터의 사용을 통해 하나의 영상특징만을 고려한 경우 발생되는 겹침 영역에서의 분할 성능 저하를 개선할 수 있다. 세포영상 분석을 위한 소프트웨어 패키지인 Cellprofiler와의 비교/분석 실험을 통해 제안 알고리즘의 효율성을 입증하였다.

• 한국간담췌외과학회지
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• 제27권2호
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• pp.195-200
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• 2023

Backgrounds/Aims: We aimed to build a machine learning tool to help predict low-grade intraductal papillary mucinous neoplasms (IPMNs) in order to avoid unnecessary surgical resection. IPMNs are precursors to pancreatic cancer. Surgical resection remains the only recognized treatment for IPMNs yet carries some risks of morbidity and potential mortality. Existing clinical guidelines are imperfect in distinguishing low-risk cysts from high-risk cysts that warrant resection. Methods: We built a linear support vector machine (SVM) learning model using a prospectively maintained surgical database of patients with resected IPMNs. Input variables included 18 demographic, clinical, and imaging characteristics. The outcome variable was the presence of low-grade or high-grade IPMN based on post-operative pathology results. Data were divided into a training/validation set and a testing set at a ratio of 4:1. Receiver operating characteristics analysis was used to assess classification performance. Results: A total of 575 patients with resected IPMNs were identified. Of them, 53.4% had low-grade disease on final pathology. After classifier training and testing, a linear SVM-based model (IPMN-LEARN) was applied on the validation set. It achieved an accuracy of 77.4%, with a positive predictive value of 83%, a specificity of 72%, and a sensitivity of 83% in predicting low-grade disease in patients with IPMN. The model predicted low-grade lesions with an area under the curve of 0.82. Conclusions: A linear SVM learning model can identify low-grade IPMNs with good sensitivity and specificity. It may be used as a complement to existing guidelines to identify patients who could avoid unnecessary surgical resection.

• 한국통신학회논문지
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• 제18권12호
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• pp.1970-1982
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• 1993

본 논문에서는 vector-radix 2차원 고속 DCT(VR-FCT)를 VLSI 병렬계산하기 위한 효율적인 어레이 알고리듬을 제안하고, 이를 집적회로로 구현하기 위한 회로를 설계하였다. VR-FCT 알고리듬의 버터플라이 연산부분을 2차원 어레이에 매핑하여 이를 병렬 및 파이프라인 처리함을써 VR-FCT 알고리듬의 고속성과 2차원 어레이의 병렬성 및 국부통신 특성을 동시에 이용할 수 있다는 특징을 갖는다. 제안된 구현방식은 RCA 방식과는 달리 transposition 메모리가 필요치 않으며, 2차원 어레이의 구조적인 규칙성, 모듈성 및 국부연결성 등에 의해 회로설계 시간의 단축, 설계검증 및 설계변경등이 용이하여 VLSI 구현에 매우 적합하다. 연산회로는 곱셈기를 사용하기않고 가산기만으로 설계하였으며, 2의 보수연산 대신에 Canonic-Signed Didit(CSD) 코드를 사용함으로써 약 30%의 가산횟수를 줄일 수 있었다. 제안된 방법의 DCT 연산과정을 C언어로 모델링하여 회로의 유한 레지스터 길이에 대한 연산정밀도를 분석하였다. 제안된 어레이 알고리듬의 시간성능은 (N*N) 2차원 DCT에 대해 O(N+Nnzd-log2N)의 시간 복잡도를 갖는다. 시뮬레이션 결과고부터 Nnzp=4이고 50MHz 클럭이 사용되는 경우, (8*8) DCT계산에 약 0.88 sec가 소요괴며, 약 72*10 pixels/sec의 연산성능이 예상된다.

• Journal of applied mathematics & informatics
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• 제34권3_4호
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• pp.239-247
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• 2016

In recent years, CA has been applied to image security due to its simple and regular structure, local interaction and random-like behavior. Since the initial state is regenerated after some iterations in the group CA, the receiver is able to decrypt by the same CA. Pries et al. showed that the all lengths of the cycles in the complemented group CA C with rules 195, 153, and 51 are equal to the order of C. Nandi et al. reported the encryption technique using C. These results can be made efficient use in cryptosystem by expanding the Nandi's key space. In this paper, we analyze the order of the complemented group CA derived from 90=150 group CA and show that all the lengths of the cycles in the complemented CA are equal to the order of the complemented CA.

• 한국멀티미디어학회논문지
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• 제6권3호
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• pp.549-555
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• 2003

LFSR보다 CA가 랜덤성이 우수한 패턴들을 효율적으로 생성함이 알려지면서 그 응용분야가 점차적으로 확대되고 있다. 특히 Nongroup CA는 해쉬함수의 생성, 암호알고리즘, 이미지 압축 등에 응용되고 있다. 본 논문에서는 TPNCA의 성질들을 분석하고, 선형 TPNCA의 0-트리의 기본경로와 순환상태의 사이클 구조를 이용하여 선형 TPNCA의 상태 전이그래프의 정확한 구조를 파악하는데 사용되던 기존의 행렬의 곱셈 연산 방법을 덧셈 연산으로 대체할 수 있음을 보였다. 또한 선형 TPNCA C의 0-트리의 비순환 상태를 여원벡터로 갖는 여원 TPNCA C'은 C와 그 구조가 동형임을 밝힘으로써 선형 TPNCA로부터 여원 TPNCA의 상태들의 위치를 정확하게 파악하여, CA를 이용하는 알고리즘을 개발하는데 있어 선행되어야 하는 CA의 상태를 분석하는 시간을 효과적으로 줄였다.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2005년도 학술대회 논문집 정보 및 제어부문
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• pp.261-263
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• 2005

In this paper, we implement an identification system using the vein image of the hand. The vein pattern is obtained in the grey-scale 2D image through the infrared-red imaging from back of the hand. Since the frame has lack of clearance, we use some enhancing methods such as the complement, addition, and multiplication to the image to increase the contrast. After Wiener filtering for smoothness of the vein pattern, we transform the image into the binary image with mean function. The binarized image is session thinned and the cross-points in the vein tree are obtained by calculating the number of pixels connected because the image is shaped as a tree. We choose the point and find the nearest to the center if it has majority, where we find the two end points of the selected line. We can get the angle between the two lines joined at the cross-point and store its coordinates, angle, and label the values. The values are used as the feature vector of the vein pattern. This procedure is similar to the human cognition sequences. It is shown that the proposed method is simple for the vein recognition.