• Journal of Microbiology and Biotechnology
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• v.19 no.7
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• pp.647-650
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• 2009

Using 200 porcine colon tissues, the efficiencies of three isolation methods of Campylobacter from porcine intestines were compared: Method 1, direct streaking of colon mucosa; Method 2, direct inoculation of intestinal contents with a swab; Method 3, inoculation of pre-enriched medium. A total of 460 Campylobacter isolates were obtained from 178 samples (89%) by direct streaking of colon mucosa, 142 samples (71%) by direct streaking of a swab, and 94 samples (47%) by pre-enrichment of intestinal contents in Preston broth. Direct streaking of colon mucosa was superior to the other two isolation methods, in terms of rapidity and higher efficiency. When isolates were identified with various biochemical tests and PCRs specific to 16s rRNA, mapA, and ceuE, C. coli was the predominant species (87%) in porcine, whereas the rest of the isolates were identified as C. lanienae.

• Earthquakes and Structures
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• v.16 no.1
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• pp.1-9
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• 2019

To provide a simplified method for the base isolation design of LNG tanks, such as $16{\times}104m^3$ LNG tanks, we conducted a derivation and calculation example analysis of the dynamic response of the base isolation of LNG storage tanks, using dynamic response analysis theory with consideration of pile-soil interaction. The ADINA finite element software package was used to conduct the numerical simulation analysis, and compare it with the theoretical solution. The ground-shaking table experiment of LNG tank base isolation was carried out simultaneously. The results show that the pile-soil interaction is not obvious under the condition of base isolation. Comparing base isolation to no isolation, the seismic response clearly decreases, but there is less of an effect on the shaking wave height after adopting pile top isolation support. This indicates that the basic isolation measures cannot control the wave height. A comparison of the shaking table experiment with the finite element solution and the theoretical solution shows that the finite element solution and theoretical solution are feasible. The three experiments are mutually verified.

• Earthquakes and Structures
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• v.11 no.1
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• pp.25-43
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• 2016

The uneven distribution of rolling friction coefficient may lead to great uncertainty in the structural seismic isolation performance. This paper attempts to improve the isolation performance of a spring-damper-rolling isolation system by artificially making the uneven friction distribution to be concave. The rolling friction coefficient gradually increases when the isolator rolls away from the original position during an earthquake. After the spring-damper-rolling isolation system under different ground motions was calculated by a numerical analysis method, the system obtained more regular results than that of random uneven friction distributions. Results shows that the concave friction distribution can not only dissipate the earthquake energy, but also change the structural natural period. These functions improve the seismic isolation efficiency of the spring-damper-rolling isolation system in comparison with the random uneven distribution of rolling friction coefficient, and always lead to a relatively acceptable isolation state even if the actual earthquake significantly differs from the design earthquake.

• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.18 no.5
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• pp.558-563
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• 2008

Vibration/shock affects biggest taking a train subtraction of vehicle and durability decline. Therefore, absorber is used for vibration/shock isolation and various qualities of the material and design are applied to isolation. This paper proposes vibration/shock absorber that applies 'Disc' spring. Through comparison with 'Disc' spring that has nonlinearity and coil spring that is having linearity, see effect that nonlinearity of isolation gets in vibration/shock Isolation. Coil spring and 'Disc' spring are non-linear numerical analysis and simulation through theory for this, get and investigate comparison result through an experiment finally. Expressed and formulated shock through 'Runge-Kutta' method/impact response to nonlinear-vibration-equation of 1 degree of freedom for numerical analysis. Double half sine pulse of excitation used and analyzed result through spectrum response analysis here. Response of disc spring is compared to response of coil spring by changing $h_o/t$ ratio with computer simulation and the usage of disc spring is increased through analysis of effect of design factors. The purpose of this paper is that the shock response of disc spring is calculated through numerical simulation and to design the optimal absorber under the limited condition. And then, the isolation effect was analyzed through the shock test.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2008.04a
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• pp.990-993
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• 2008

Vibration/shock affects biggest taking a train subtraction of vehicle and durability decline. Therefore, absorber is used for vibration/shock isolation and various qualities of the material and design are applied to isolation. This paper proposes vibration/shock absorber that applies 'Disc'spring. Through comparison with 'Disc' spring that has nonlinearity and coil spring that is having linearity, see effect that nonlinearity of isolation gets in vibration/shock isolation. Coil spring and 'Disc' spring are non-linear numerical analysis and simulation through theory for this, get and investigate comparison result through an experiment finally. Expressed and formulated shock through 'Runge-Kutta' method/impact response to nonlinear-vibration-equation of 1 degree of freedom for numerical analysis. Double half sine pulse of excitation used and analyzed result through spectrum response analysis here. Response of disc spring is compared to response of coil spring by changing ho/t ratio with computer simulation and the usage of disc spring is increased through analysis of effect of design factors. The purpose of this paper is that the shock response of disc spring is calculated through numerical simulation and to design the optimal absorber under the limited condition. And then, the isolation effect was analyzed through the shock test.

• Proceedings of the Earthquake Engineering Society of Korea Conference
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• 2005.03a
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• pp.453-460
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• 2005

In this study, the seismic fragility analysis of a base isolated condensate storage tank installed in the nuclear power plant. The condensate storage tank is safety related structure in a nuclear power plant. The failure of this tank affect significantly to the core damage frequency of the nuclear power plants. The seismic analysis of the liquid storage tank was performed by the simple calculation method and the dynamic time storage analysis method. The convective and impulsive fluid mass is modeled as added masses proposed by several researchers. To evaluate the effectiveness of the isolation system, the comparison of HCLPF and core damage frequencies in non-isolated and isolated cases are carried out. It can be found from the results that the seismic isolation system increases the seismic capacity of a condensate storage tank and decreases the core damage frequency significantly.

• Korean Journal of Food Science and Technology
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• v.48 no.4
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• pp.335-340
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• 2016

This study was conducted to find out the most suitable DNA isolation methods for PCR detection of foodborne pathogens. Four DNA isolation methods including Universal Genomic DNA Extraction Kit (TaKaRa), PrepMan Ultra (Applied Biosystems), boiling method and alkaline lysis method (w/PEG) were tested and compared. The Universal Genomic DNA Extraction kit (TaKaRa) was considered as the more efficient isolation method for Escherichia coli O157:H7 and Staphylococcus aureus in lettuce, fish and beef. Meanwhile to detect the foodborne pathogens directly from foods without enrichment, the four different buffers such as double-distilled water, saline, glycine-saline, glycine-saline with Tween-20 and beef extract were also evaluated. As a result, saline was more suitable buffer for E. coli O157:H7. And double-distilled water was more suitable buffer than saline for S. aureus, respectively

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2005.05a
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• pp.275-278
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• 2005

In this study, the dynamic properties of a floor impact sound isolation pad expressed in terms of the natural frequency, the dynamic stiffness per unit area and the loss factor are measured by the resonant method. By using the measured dynamic properties, the vibration transmissibility diagram is obtained for each isolation pad, which is compared with the values tested by the impact sound sources at the room in an apartment. From the comparative results, it is found that the noise reduction Performances. of isolation pads are closely connected with the natural frequency and the dynamic stiffness per unit area.

• Journal of Electrical Engineering and Technology
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• v.3 no.4
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• pp.484-490
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• 2008

In this paper, an integrated fault section detection and isolation strategy is proposed based on the application of the Distribution Automation System(DAS) utilizing advanced IT and communication technologies. The Feeder Remote Terminal Unit(FRTU) has been widely used to collect data in the Korean distribution system. The achieved data is adopted in this method for detecting multiple fault types. Especially in the case of single phase-to-ground fault, the fault section is detected by comparison of the zero-sequence current phase angle. The test results have verified the effectiveness of the proposed method in a radial distribution system through extensive simulations in Matlab/Simulink. Furthermore, a communication-based demo system identical to the simulation model has been developed, and it can be applied as an online monitoring and control program for fault section detection and isolation.

• Journal of Embryo Transfer
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• v.33 no.4
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• pp.327-336
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• 2018

To date, there are no protocols optimized to the effective separation of spermatogonial stem cells (SSCs) from testicular cells derived from mouse testes, thus hindering studies based on mouse SSCs. In this study, we aimed to determine the most efficient purification method for the isolation of SSCs from mouse testes among previously described techniques. Isolation of SSCs from testicular cells derived from mouse testes was conducted using four different techniques: differential plating (DP), magnetic-activated cell sorting (MACS) post-DP, MACS, and positive and negative selection double MACS. DP was performed for 1, 2, 4, 8, or 16 h, and MACS was performed using EpCAM ($MACS^{EpCAM}$), Thy1 ($MACS^{Thy1}$), or GFR ${\alpha}1$ ($MACS^{GFR{\alpha}1}$) antibodies. The purification efficiency of each method was analyzed by measuring the percentage of cells that stained positively for alkaline phosphatase. DP for 8 h, $MACS^{Thy1}$ post-DP for 8 h, $MACS^{GFR{\alpha}1}$, positive selection double $MACS^{GFR{\alpha}1/EpCAM}$, and negative selection double $MACS^{GFR{\alpha}1/{\alpha}-SMA}$ were identified as the optimal protocols for isolation of SSCs from mouse testicular cells. Comparison of the purification efficiencies of the optimized isolation protocols showed that, numerically, the highest purification efficiency was obtained using $MACS^{GFR{\alpha}1}$. Overall, our results indicate that $MACS^{GFR{\alpha}1}$ is an appropriate purification technique for the isolation of SSCs from mouse testicular cells.