• Journal of The Korean Society of Grassland and Forage Science
• /
• v.34 no.3
• /
• pp.187-192
• /
• 2014

Alfalfa (Medicago sativa L.) is one of the most important forage legumes in the world. It has been demanded to establish the efficient transformation system in commercial varieties of alfalfa for forage molecular breeding and production of varieties possessing new characteristics. To approach this, genetic transformation techniques have been developed and modified. This work was performed to establish conditions for effective transformation of commercial alfalfa cultivars, Xinjiang Daye, ABT405, Vernal, Wintergreen and Alfagraze. GUS gene was used as a transgene and cotyledon and hypocotyl as a source of explants. Transformation efficiencies differed from 0 to 7.9% among alfalfa cultivars. Highest transformation efficiencies were observed in the cultivar Xinjiang Daye. The integration and expression of the transgenes in the transformed alfalfa plants was confirmed by polymerase chain reaction (PCR) and histochemical GUS assay. These data demonstrate highly efficient Agrobacterium transformation of diverse alfalfa cultivars Xinjiang Daye, which enables routine production of transgenic alfalfa plants.

• Asian Journal of Turfgrass Science
• /
• v.5 no.1
• /
• pp.23-32
• /
• 1991

The purpose of this study was to determine the vegetative growth of two cool season turfgrasses during summer season in Korea. Ten respective cultivars of Kentucky bluegrass [Poapratensis L.] and perennial ryegrass [Lolium perenne L.] were exposed to unshaded, 30% and 60% shade for 4odays. First mowing height was 7cm, and then clippings were harvested every ten days at the same height. Shading increased the total length of clippings of all cultivars of two turfgrasses, but fresh and dry weight were significantly decreased. The highest shoot length obtained at 30% shade in Kentucky bluegrass and at 60% shade in perennial ryegrass. 'Rugky', 'Sydsport', 'Wabash' and 'Baron' of Kentucky bluegrass and 'Repel', 'Omega II', 'Ovation', 'Linn', 'Bell' and 'Manhattan' of perennial ryegrass showed relatively high tolerance to shade. Especially, in 'Citation II' perennial ryegrass, fresh and dry weight were increased by 30% shade. The number of tillers generally decreased with increasing shade. In 30% shade, 'Midnight' Kentucky bluegrass and 'Omega II' perennial ryegrass were least reduced and 'Baron' Kentucky bluegrass and 'Pennant' perennial ryegrass were most reduced. The cultivars of lower growth habit in full sunlight showed shade tolerance compared to those of higher growth habit.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.12 no.4
• /
• pp.232-238
• /
• 1992

To understand the basic information on the management and utilization of reed canarygrass (Phalaris arundinacea L.), four cultivars of Palaton. Venture, Castor and Frontier (control) were compared for growth characteristics, dry matter (DM) yield and nutritive value, 1990 to 1992. The reed canarygrass was harvested four times annually, at soiling stage. The average plant height at harvest was 60 cm in Frontier, 57 cm in Palaton and Venture, and 52 cm in Castor. Initial heading and heading date in 1990 were ca. 21 May and 29 May, respectively, regardless of cultivar. The highest yield (12.495 kg/ha) in 1990 was observed by Frontier. In 1991. Palaton. Venture and Frontier outyielded Castor significantly, and the yields of all cultivars in 1992 were not significant, ranged from 11,460 kg (Castor) to 12,298 kg/ha (Palaton). On an average for three years, Palaton (10,592 kg), Venturc (10,405 kg) and Frontier (1 1,277 kg/ha) produced more forages than Castor. In Frontier the percentage of DM distribution at first harvest (spring) was highest (32.4%). However, the distributions at third (summer) and fourth (autumn) harvest were high in Palaton and Venture, compared with Frontier. The nutritive value of reed canarygrass was not significantly different among cultivars, and the percentage of weeds was slightly high in Castor reed canarygrass.

• Journal of Nutrition and Health
• /
• v.37 no.1
• /
• pp.45-51
• /
• 2004

In this study, isoflavone (genistein, genistin, daidzein, daidzin) contents in various parts of twelve soybean cultivars and three soy sprouts were determined by high performance liquid chromatography with UV detector. Three cultivars of soybean were selected and cultured in the lab to produce sprout for five days. Total isoflavone (Total IF) varied greatly among differnt breeds of soybean in range of 99 - 649.9 $\mu\textrm{g}$/g and 522.3 - 1,277.7 $\mu\textrm{g}$/g respectively, domestic and foreign cultivars. There were greatly difference in total IF of various parts of the soybean sprouts. Sprout from the Myunjunamul-kong appeared to have 69% genistein and 22% genistin in head part, and 30% and 62% of daidzin and daidzein, respectively, in root. Meanwhile, the sprouts from Junjori contains most (84%) of daidzein in its root. Sprout from chinese black-soybean had the largest amount of genistein among the sprouts but, there were no differences in the average genistein content between three selected black and non-black soys. The glycosidic form of IF were dominant compared to aglycone forms both in soybean and sprouts by 24 times and 12 times, respectively, suggesting that during the sprouts cultivation glycosidic forms could change to aglycone forms. There are no difference in total content between genistein + genistin and daidzein + daidzin in soy and soy sprout. Therfore, considering the total IF contents, the intake of 1 soy sprout is similer to 1.5 times as soybean.

• Korean Journal of Organic Agriculture
• /
• v.21 no.1
• /
• pp.61-68
• /
• 2013

This study was conducted to evaluate overwintering of hairy vetch (Vicia villosa Roth), biomass, and inorganic N concentration in soil to verify the adaptability of eight cultivars of hairy vetch into country. Winter survival rate was higher for the hairy vetch cultivars, such as Hungvillosa or Ostsaat, than those of Minnie, Oregon common, and TTF1, which affected amount of biomass of each hairy vetch, with higher biomass observed in Hungvillosa or Ostsaat cultivar. There were no significant difference for the mineral nutrients of each hairy vetch cultivar. Inorganic N concentration in soil was increased at 10 days after mowing by application of Hungvillosa and Ostsaat cultivars that had highly produced N content from the raw materials.

• Horticultural Science & Technology
• /
• v.16 no.4
• /
• pp.528-530
• /
• 1998

The hardwood cutting of Hibiscus syriacus was studied by using 54 different cultivars. In the evaluation of rooting ability and shoot growth, there were much differences between cultivars or groups classified by flower types. The outstanding rooting ability was found in cultivars such as 'Comte de Haimont', 'Hanol-tanshim', 'Hyangdan', 'Nabora', 'Saimdang', whereas 'Ilpyontanshim', 'Lucy', 'Paedal', 'Sohoyang' and other several cultivars showed poor characteristics in their rooting. The longest shoot was observed in 'Hanoltanshim', 'Kreider Blue', 'Nabora', 'New Hamabo', 'Nunbora', while 'Chossarang', 'Lucy', 'Shintaeyang', 'Youngkwang', etc. showed very short shoots. As a result of comparison among three different flower types, semi-doubled flower type showed the most extent of rooting ability and shoot growth.

• Korean Journal of Medicinal Crop Science
• /
• v.27 no.5
• /
• pp.330-338
• /
• 2019

Background: This study was conducted to compare the physicochemical properties of 12 different cultivars of Rehmannia glutinosa, a plant used in Korean traditional herbal medicine. Method and Results: We investigated the following 12 cultivars of R. glutinosa; Jihwang1, Goryeo, Daegyung, Gogang, Togang, Dagang, Wongang, Yeongang, Hwanggang, Dahwang, Segang and Chunggang. We measured water content, chromaticity, hardness, active components levels, and antioxidant activity. Our results showed that Togang has the lowest water content and the highest yield. The catalpol content was the highest in Togang (40.50 mg/g) and the lowest in Chunggang (22.17 mg/g). The aucubin content was the highest in Hwanggang (0.28 mg/g). Chunggang had the lowest aucubin content, but the highest level of verbascoside. Total flavonoid and total polyphenol were the highest in Daegyung and Chunggang, respectively. There was a strong correlation between the total flavonoid and total polyphenol (r = 0.89) content. Conclusions: There were significant differences in physicochemical properties among 12 cultivars. These properties will affect suitability for processing and final product quality. We therefore suggest the selection of appropriate cultivars for different uses.

• Journal of the Korean Society of Environmental Restoration Technology
• /
• v.18 no.3
• /
• pp.23-38
• /
• 2015

The study was initiated with Kentucky bluegrass (KB, Poa pratensis L.) to investigate germination and early establishment characteristics of new cultivars for a practical application to turfgrass establishment such as parks, athletic field and golf course etc. Fifteen cultivars were evaluated in different experiments. An alternative condition for a KB germination test required by International Seed Testing Association (ISTA) was applied in Experiment I, consisting of 8-hr light at $25^{\circ}C$ and 16-hr dark at $15^{\circ}C$ (ISTA conditions). Experiment II was conducted under a room temperature condition of 5 to $25^{\circ}C$ (natural conditions). Seed germinating vigor, germination speed and germination peak time were measured in both experiments. Significant differences were observed in seed germinating vigor, germination speed and germination peak time. Seed germinating vigor was variable with different environments and cultivars. It was 75.25 to 89.50% under ISTA conditions and 75.75 to 90.25% under natural conditions. There were considerable variations in early germination characteristics among KB cultivars according to different environments. Early germination characteristics showed that all cultivars were 3 to 5 days faster in germination under ISTA conditions, when compared with natural conditions. The germination speed, measured as days to seed germination of 50% to 80%, was much faster with 'Midnight II', 'Excursion' and 'Midnight' under ISTA conditions. But it was faster with 'Midnight II', 'Excursion' and 'Odyssey' under natural conditions. Differences was also observed in germination peak time with cultivars and growing conditions. It ranged 5.94 to 14.88 days under ISTA conditions and 4.71 to 13.06 days under natural conditions. Regardless of the environment conditions, the shortest cultivars were 'Odyssey' and 'Midnight II'. The longest ones were 'Nuglade' under ISTA condition and 'Moonlight' under natural conditions. Considering germinating vigor, germination speed and germination peak time, 'Midnight II', 'Excursion', 'Midnight', and 'Odyssey' were regarded as excellent cultivars under ISTA conditions in terms of early establishment characteristics, while 'Midnight II', 'Excursion', 'Odyssey', and 'Courtyard' under natural conditions. These results suggest that an intensive germination test be needed prior to planting, for investigating the early germinating vigor, germination speed and germination peak time. Also, a proper cultivar selection and comprehensive site analysis for the growing environmental conditions should be done before turfgrass establishment such as golf course construction.

• Horticultural Science & Technology
• /
• v.17 no.2
• /
• pp.111-114
• /
• 1999

Experiments were conducted to select the optimum cultivars which are adaptable to South Korea through estimating the differences in daughter plant production and growth response among seven strawberry cultivars. In the vegetative stage, Nyoho, Akihime and Suhong produced more daughter plants than other cultivars whereas Akaneko and Tochinomine, which developed less runners, showed better condition in runner vigorness. The quality of daughter plants derived from forcing cultivars was uniform in general. Even though there was no significant differences in rooting among cultivars, runners from Akaneko and Tochinomine developed more roots and fresh weight of roots than other cultivars. In the forcing cultivars, Akihime showed the most excellent plant vigorness, yield and highest sugar content of fruits and also was 5 days faster than Nyoho in flowering date. However, the fruit tended to be softened. In the semi-forcing cultivars, Tochinomine was excellent in vigorness, firmness, sugar content of fruits and the average of fruit weight but low in flower numbers per cluster and late in the emergency of second flower cluster. Although Akaneko showed highest in sugar/acid ratio and rich in flavour, lighting treatment was recommended to this cultivar for early harvest because of softness of fruits.

• The Korean Journal of Food And Nutrition
• /
• v.36 no.6
• /
• pp.543-550
• /
• 2023

Mulberry fruit is a superior source of polyphenols, especially anthocyanin, and has a long history of use as an edible fruit and traditional medicine. The anthocyanin composition of mulberry fruit from 15 Korean cultivars was analyzed by ultra-performance liquid chromatography diode array detector with quadrupole time of flight/mass spectrometry (UPLC-DAD-QToF/MS) based on a cyanin internal standard. The four glycosides were identified by comparison with authentic standards and published reports. The major anthocyanin was cyanidin 3-O-glucoside (71.7%), followed by cyanidin 3-O-rutinoside (26.6%). The minor components (total of 1.7%) were pelargonidin 3-O-glucoside and pelargonidin 3-O-rutinoside. The total anthocyanin content (mg/100 g, dry weight) of mulberry fruit varied by cultivar and ranged from 471.5±4.0 (Su Hong) to 4,700.2±54.0 (Gwa Sang2). Among the 15 cultivars examined, Gwa Sang2 showed the highest level of cyanidin 3-O-glucoside (3,133.4±32.6), which was 9-fold higher than that of Su Hong (351.5±3.4). In conclusion, anthocyanin profiles, including pelargonidin 3-O-glucoside and pelargonidin 3-O-rutinoside, were reported for the first time from 15 Korean mulberry fruit cultivars. The results will contribute valuable information on pharmaceutical properties, breeding superior mulberry cultivars, and food industries.