• Title/Summary/Keyword: comparison of body types

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Development of an Integrated General Model (IGM) System for Comparison of Genetic Gains from Different Bull Selection Strategies for Korean Brown Cattle (Hanwoo)

  • Lee, Jeong-Soo;Kim, Hee-Bal;Kim, Si-Dong
    • Asian-Australasian Journal of Animal Sciences
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    • v.24 no.11
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    • pp.1483-1503
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    • 2011
  • To advance the effectiveness of the current Hanwoo improvement system, we developed a general simulation that compared a series of breeding schemes under realistic user circumstances. We call this system the Integrated General Model (IGM) and it allows users to control the breeding schemes and selection methods by manipulating the input parameters. The Current Hanwoo Performance and Progeny Test (CHPPT) scheme was simulated with a Modified Hanwoo Performance and Progeny Test (MHPPT) scheme using a Hanwoo Breeding Farm cow population of the Livestock Improvement Main Center (LOMC) of the National Agricultural Cooperatives Federation (NACF). To compare the two schemes, a new method, the Simple Hanwoo Performance Test (SHPT), which uses ultrasound technology for measuring the carcass traits of live animals, was developed. These three models, including the CHPPT, incorporated three types of selection criteria: phenotype (PH), true breeding value (TBV), and estimated breeding value (EBV). The simulation was scheduled to mimic an actual Hanwoo breeding program; thus, the simulation was run to include the years 1983-2020 for each breeding method and was replicated 10 times. The parameters for simulation were derived from the literature. Approximately 642,000 animals were simulated per replication for the CHPPT scheme; 129,000 animals were simulated for the MHPPT scheme and 112,000 animals for the SHPT scheme. Throughout the 38-year simulation, all estimated parameters of each simulated population, regardless of population size, showed results similar to the input parameters. The deviations between input and output values for the parameters in the large populations were statistically acceptable. In this study, we integrated three simulated models, including the CHPPT, in an attempt to achieve the greatest genetic gains within major economic traits including body weight at 12 months of age (BW12), body weight at 24 months of age (BW24), average daily gain from 6 to 12 months (ADG), carcass weight (CWT), carcass longissimus muscle area (CLMA), carcass marbling score (CMS), ultrasound scanned longissimus muscle area (ULMA), and ultrasound scanned marbling score (UMS).

Comparison of Health Risks according to the Obesity Types Based Upon BMI and Waist Circumference in Korean Adults: The 1998-2005 Korean National Health and Nutrition Examination Surveys (한국 성인에서 체질량지수와 허리둘레를 기준으로 분류한 비만 유형에 따른 특성 비교: 1998-2005 국민건강영양조사)

  • Lee, Yul-Eui;Park, Ji-Eun;Hwang, Ji-Yun;Kim, Wha-Young
    • Journal of Nutrition and Health
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    • v.42 no.7
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    • pp.631-638
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    • 2009
  • This study was performed to examine the health risks according to the obesity types based upon body mass index (BMI) and waist circumference (WC) cutoffs. The subjects were 8,966 adults aged 40-79 years from the 1998, 2001 and 2005 Korean National Health and Nutrition Examination Surveys. The subjects were classified into 4 types of obesity groups based on BMI and WC: BMIWC (BMI${\geq}$ 25 kg/$m^2$ and WC${\geq}$ 90 cm for males and ${\geq}$ 85 cm for females), WC (BMI < 25 kg/$m^2$ and ${\geq}$ 90 cm for males and ${\geq}$ 85 cm for females), BMI (BMI ${\geq}$ 25 kg/$m^2$ and < 90 cm for males and < 85 cm for females), normal (BMI < 25 kg/$m^2$ and < 90 cm for males and < 85 cm for females) groups. More than half (n = 5,103) of the subjects fell into the normal group and BMIWC group accounts for 58%, followed by BMI group of 27% and WC group of 16%. Mean ages of subjects were highest in WC group and lowest in BMI group. Blood levels of total cholesterol, triglyceride, LDL-cholesterol and glucose, and blood pressures were higher and HDL-cholesterol was lower in 3 types of obesity groups (BMIWC, WC, BMI) than in the normal group. Among the 3 obesity groups, energy and fat intake was significantly higher in BMIWC than in normal group in males, however, no differences were found among the 4 groups in females. The three obesity groups had greater odds ratios for hypertension, diabetes mellitus, hypercholesterolemia, hyper-LDL-cholesterolemia, hypo-HDL-cholesterolemia, hypertriglyceridemia, and metabolic syndrome compared to the normal group. Among 3 obesity groups, BMIWC group had the highest odds ratios for hypertension, diabetes mellitus, and metabolic syndrome. Especially BMIWC and WC groups showed the highest odds ratios for diabetes mellitus and metabolic syndrome, suggesting the potential role of abdominal obesity in diabetes mellitus and metabolic syndrome. Our data indicate that different types of obesity may show different degrees of health risks. The appropriate selection and use of obesity indexes may be effective for management of obesity as well as obesityrelated diseases.

Comparison between Diagnostic Results of the Sasang Constitutional Analysis Tool (SCAT) and a Sasang Constitution Expert (체질점수 복용첩수 내원회수 연령에 따른 사상체질진단툴(SCAT)과 전문가의 체질진단 일치도 연구)

  • Do, Jun-Hyeong;Nam, Ji-Ho;Jang, Eun-Su;Jang, Jun-Su;Kim, Jang-Woong;Kim, Young-Su;Kim, Na-Hye;Han, Soon-Man;Kim, Jong-Dug
    • Journal of Sasang Constitutional Medicine
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    • v.25 no.3
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    • pp.158-166
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    • 2013
  • Objectives Recently, Sasang Constitutional Analysis Tool (SCAT) was developed using face, body shape, questionnaire, and voice information. The purpose of this paper is to analysis the characteristics of SCAT in detail for the subjects whose Sasang Constitutional (SC) type was confirmed clearly. Methods 1) The SC types of 108 patients were diagnosed by an expert of Sasang constitutional medicine through the observation of improvements after taking more than 20 packs of herbal medicine prescribed 2) The diagnostic results of SCAT were acquired for the patients. 3) Comparison between diagnostic results of the SCAT and expert was performed according to SC probability difference, the number of packs of herbal medicine prescribed, the number of hospital visits, and age. Results 1) The concordance rate was 65.9% and 59.4% in the male and female groups, respectively. 2) Overall, the concordance rate increased as the SC probability difference increased. In case of more than 5% SC probability difference, it increased up to 71.0% and 66.7% in the male and female groups, respectively. 3) The concordance rate did not show any trend as the number of packs of herbal medicine prescribed and the number of hospital visits increased. 4) The higher concordance rate was observed in Taeeumin, Soyangin, and 20s ~ 40s groups of male patients, and in Soyangin, 20s, and 40s groups of female patients. Conclusions The strong and weak points of SCAT were observed and they will contribute to the improvement of SCAT performance.

A Study on the Comparison of Foot Type for the Men in Their 20's and 60's - With the Usage of Size Korea's 3-Dimensional Measurement Data - (20대와 60대 남성의 발 유형 비교 연구 - Size Korea의 3차원 측정 데이터를 이용하여 -)

  • Seok, Hye-Jung;Park, Ji-Eun;Han, Seung-Hee;Kim, Deuk-Ha
    • Journal of the Korea Fashion and Costume Design Association
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    • v.11 no.2
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    • pp.183-195
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    • 2009
  • In this study, foot shapes were compared between the men in their 20's and 60's, with the application of 3-dimensional measurement data, and the classification was established in consideration of each of their features. The results are as follows: 1. The investigation into the differences between the groups for the 3-dimensional measurement items of the men in their 20's and 60's indicated that those in their 20's had larger values than those in the 60's age group for all of the length items, while those in the 60's age group had larger values for all of the angle items. 2. The analysis of the constituting factors for the feet of those in their 60's and 20's resulted in the 7 items for the 20's age group and 8 items for those in the 60's age group. 3. The comparison of foot classifications for the 60's age group and 20's age group presented 3 types each for both groups, but the features of each type was different. To examine each characteristic by each type and age group, following can be determined of the 60's age group. Type 1 had a thick instep, high malleolus position and heel and a thick formed ankle. Type 2 the entire foot was small, and the big toe was formed in such a way that it tened to bend toward the inside. When the whole size compared with similar Type 1, Type 3 had a low instep, a low heel, the big toe did not bend and ankle form was thin. The following can be determined of the 20's age group. Type 1 had narrow width a thin ankle and the big toe was formed in such a way that it bended toward the inside. Compared with length, Type 2 had wide width and a thick ankle. Type 3 had a larger overall size, thick instep and wide and the toe was big, but the characteristic of inside and outside of a malleolus position was low and the big toe's form showed no tendencies to bend.

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Phylogenetic and Morphological Comparison between Thamnaconus septentrionalis and T. modestus Collected in Southwest Seashore (서남해에서 채집된 말쥐치 (Thamnaconus modestus)와 유사종 (T. septentrionalis)의 형태 및 계통유전학적 비교)

  • Yu, Tae-Sik;Park, Kiyun;Han, KyeongHo;Kwak, Ihn-Sil
    • Korean Journal of Ecology and Environment
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    • v.54 no.3
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    • pp.229-239
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    • 2021
  • Thamnaconus modestus, distributed in the Northwest Pacific, has high economic value and is used in various seafood. In this study, the morphological and genetic characteristics of T. modestus and T. septentrionalis were compared and analyzed. We observed the external and internal morphology of T. modestus, sketched skeletal elements, and analyzed phylogenetic evolutionary relationships using the cytochrome c oxidase subunit I (COI) gene on mitochondrial DNA compared to T. septentrionalis. The T. modestus observed in this study had blackish-brown patterns irregularly scattered on the gray-brown body, and the fins were blue-green. Genetic analysis results based on the COI sequences of T. modestus showed seven types of base sequence variation; however, the homology was more than 98.8%. In addition, as a result of comparison of the COI nucleotide sequences and phylogenetic analysis in Tetraodontiformes, two T. septentrionalis sequences (JN813099, MW485059) were similar to T. modestus with 99% homology, and the other two T. septentrionalis sequences (EF607583, KP267619) were similar to those of species belonging to another genus Thamnaconus with 95% homology with T. modestus. It was not easy to classify the species based on morphological characteristics, and phylogenetic analysis between T. modestus and T. septentrionalis confirmed the difference in classification. These results provide the external and internal morphology of T. modestus and will be used as important information for the taxonomic study of T. modestus and T. septentrionalis.

A Case Study on the Maintenance Activities of the Naturally Favorable Water Space Through the Participation of Residents - A case of Japanese rural areas - (주민참여에 의한 농촌지역 친수공간 유지관리활동 사례분석 - 일본 농촌지역을 사례로 -)

  • Yang, Yong-Seok;Kim, Sun-Joo;Senga, Yutaro
    • Journal of Korean Society of Rural Planning
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    • v.12 no.1 s.30
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    • pp.11-21
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    • 2006
  • The purpose of this study is to verify the result of the previous studies and to clarify the types and activation factors of maintenance activities of the naturally favorable water space through the participation of residents in rural areas, through a further survey of the area proposed as a program in the existing study review and previous study related to the participation of residents. The survey was conducted through the survey of 1,242 households and 1,335 persons intended for Japanese rural areas. The study result is summarized as follows. The types of the maintenance activities in the areas intended for the survey have been classified into 'rural type' and 'combination type' through the analysis results by the references like the comparison analysis, job percentage and main utilization purpose of facilities for each type of maintenance and operation activities. In case of the 'rural type' area, residents were doing maintenance activities centered on the group activities rather than personal maintenance activities. Also, the activities of maintenance & operation group members were more active than non-members, and the water-friendly utilization to the facilities was not directly affecting the maintenance activities of residents. In case of the 'combinational type' area, there was no common characteristic according to each area, and the participation percentage in the maintenance activities was low compared with the 'rural type' area. According to the analysis result based on the participation degree of personal maintenance activities, the repair of facilities as a naturally favorable water space, implementation of projects reflecting the opinions of residents, educational programs conducted in order to motivate the participation of residents, etc. have been analyzed as the factors affecting the activation of maintenance activities of residents. Through the above results, for continuous maintenance activities to be made with residents as a main body in the future, it is necessary to support maintenance groups on an administrative level and procure experts for the production and operation of programs that can increase the participation percentage of residents.

Comparative accuracy of implant impression techniques with different splinting materials (임플란트 인상채득 방법과 인상용 코핑 연결 고정에 따른 정확성 비교)

  • Ki-Yoon Hong;Soo-Yeon Shin
    • Journal of Dental Rehabilitation and Applied Science
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    • v.39 no.1
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    • pp.9-20
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    • 2023
  • Purpose: We investigated the effect of open tray impression using pick-up impression coping and close tray impression using transfer impression coping on the accuracy of impression in edentulous patients on their mandibular parts. the effect of material types of pick-up type impression copings for splinting in open tray impression on the accuracy of impression was also evaluated. Materials and Methods: Two implant fixtures were implanted in parallel in the left molar of the mandibular in the shape of a mandibular partial edentulous model. The 40 individual trays were fabricated using 3D printer. The prepared individual trays were classified into 4 groups (i.e., PN, PG, PH, and TN groups), and a total of 40 impression-takings were conducted. A master cast was connected to a Scan Body. The converted STL file was super-imposed on the scan images of the various groups. Results: The order of standard deviation values decreased as follows: PN (0.2343 ± 0.0844 mm), TN (0.2192 ± 0.0840 mm), indicating that the high accuracy of impression for TN group. In addition, for the comparison results between the material types used in splinting the open tray impression, the PH group showed a relatively lower standard deviation (0.1910 ± 0.1176 mm) than that of the PN group (0.2343 ± 0.0844 mm), PG group (0.2556 ± 0.1082 mm). Conclusion: The acrylic resin synthesized by light-induced polymerization exhibited a higher accuracy of impression taking than that of autopolymerizing acrylic resin. Meanwhile, the accuracy of impression taking was not dependent on the implant impression taking method or the presence of connection/fixation of impression copings.

A Study of The Medical Classics in the '$\bar{A}yurveda$' ('아유르베다'($\bar{A}yurveda$)의 의경(醫經)에 관한 연구)

  • Kim, Ki-Wook;Park, Hyun-Kuk;Seo, Ji-Young
    • Journal of Korean Medical classics
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    • v.20 no.4
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    • pp.91-117
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    • 2007
  • Through a simple study of the medical classics in the '$\bar{A}yurveda$', we have summarized them as follows. 1) Traditional Indian medicine started in the Ganges river area at about 1500 B. C. E. and traces of medical science can be found in the "Rigveda" and "Atharvaveda". 2) The "Charaka" and "$Su\acute{s}hruta$(妙聞集)", ancient texts from India, are not the work of one person, but the result of the work and errors of different doctors and philosophers. Due to the lack of historical records, the time of Charaka or $Su\acute{s}hruta$(妙聞)s' lives are not exactly known. So the completion of the "Charaka" is estimated at 1st${\sim}$2nd century C. E. in northwestern India, and the "$Su\acute{s}hruta$" is estimated to have been completed in 3rd${\sim}$4th century C. E. in central India. Also, the "Charaka" contains details on internal medicine, while the "$Su\acute{s}hruta$" contains more details on surgery by comparison. 3) '$V\bar{a}gbhata$', one of the revered Vriddha Trayi(triad of the ancients, 三醫聖) of the '$\bar{A}yurveda$', lived and worked in about the 7th century and wrote the "$A\d{s}\d{t}\bar{a}nga$ $A\d{s}\d{t}\bar{a}nga$ $h\d{r}daya$ $sa\d{m}hit\bar{a}$ $samhit\bar{a}$(八支集)" and "$A\d{s}\d{t}\bar{a}nga$ Sangraha $samhit\bar{a}$(八心集)", where he tried to compromise and unify the "Charaka" and "$Su\acute{s}hruta$". The "$A\d{s}\d{t}\bar{a}nga$ Sangraha $samhit\bar{a}$" was translated into Tibetan and Arabic at about the 8th${\sim}$9th century, and if we generalize the medicinal plants recorded in each the "Charaka", "$Su\acute{s}hruta$" and the "$A\d{s}\d{t}\bar{a}nga$ Sangraha $samhit\bar{a}$", there are 240, 370, 240 types each. 4) The 'Madhava' focused on one of the subjects of Indian medicine, '$Nid\bar{a}na$' ie meaning "the cause of diseases(病因論)", and in one of the copies found by Bower in 4th century C. E. we can see that it uses prescriptions from the "BuHaLaJi(布哈拉集)", "Charaka", "$Su\acute{s}hruta$". 5) According to the "Charaka", there were 8 branches of ancient medicine in India : treatment of the body(kayacikitsa), special surgery(salakya), removal of alien substances(salyapahartka), treatment of poison or mis-combined medicines(visagaravairodhikaprasamana), the study of ghosts(bhutavidya), pediatrics(kaumarabhrtya), perennial youth and long life(rasayana), and the strengthening of the essence of the body(vajikarana). 6) The '$\bar{A}yurveda$', which originated from ancient experience, was recorded in Sanskrit, which was a theorization of knowledge, and also was written in verses to make memorizing easy, and made medicine the exclusive possession of the Brahmin. The first annotations were 1060 for the "Charaka", 1200 for the "$Su\acute{s}hruta$", 1150 for the "$A\d{s}\d{t}\bar{a}nga$ Sangraha $samhit\bar{a}$", and 1100 for the "$Nid\bar{a}na$", The use of various mineral medicines in the "Charaka" or the use of mercury as internal medicine in the "$A\d{s}\d{t}\bar{a}nga$ Sangraha $samhit\bar{a}$", and the palpation of the pulse for diagnosing in the '$\bar{A}yurveda$' and 'XiZhang(西藏)' medicine are similar to TCM's pulse diagnostics. The coexistence with Arabian 'Unani' medicine, compromise with western medicine and the reactionism trend restored the '$\bar{A}yurveda$' today. 7) The "Charaka" is a book inclined to internal medicine that investigates the origin of human disease which used the dualism of the 'Samkhya', the natural philosophy of the 'Vaisesika' and the logic of the 'Nyaya' in medical theories, and its structure has 16 syllables per line, 2 lines per poem and is recorded in poetry and prose. Also, the "Charaka" can be summarized into the introduction, cause, judgement, body, sensory organs, treatment, pharmaceuticals, and end, and can be seen as a work that strongly reflects the moral code of Brahmin and Aryans. 8) In extracting bloody pus, the "Charaka" introduces a 'sharp tool' bloodletting treatment, while the "$Su\scute{s}hruta$" introduces many surgical methods such as the use of gourd dippers, horns, sucking the blood with leeches. Also the "$Su\acute{s}hruta$" has 19 chapters specializing in ophthalmology, and shows 76 types of eye diseases and their treatments. 9) Since anatomy did not develop in Indian medicine, the inner structure of the human body was not well known. The only exception is 'GuXiangXue(骨相學)' which developed from 'Atharvaveda' times and the "$A\d{s}\d{t}\bar{a}nga$ Sangraha $samhit\bar{a}$". In the "$A\d{s}\d{t}\bar{a}nga$ Sangraha $samhit\bar{a}$"'s 'ShenTiLun(身體論)' there is a thorough listing of the development of a child from pregnancy to birth. The '$\bar{A}yurveda$' is not just an ancient traditional medical system but is being called alternative medicine in the west because of its ability to supplement western medicine and, as its effects are being proved scientifically it is gaining attention worldwide. We would like to say that what we have researched is just a small fragment and a limited view, and would like to correct and supplement any insufficient parts through more research of new records.

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A Study of The Medical Classics in the '$\bar{A}yurveda$' (아유르베다'($\bar{A}yurveda$) 의경(醫經)에 관한 연구)

  • Kim, Kj-Wook;Park, Hyun-Kuk;Seo, Ji-Young
    • The Journal of Dong Guk Oriental Medicine
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    • v.10
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    • pp.119-145
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    • 2008
  • Through a simple study of the medical classics in the '$\bar{A}yurveda$', we have summarized them as follows. 1) Traditional Indian medicine started in the Ganges river area at about 1500 B. C. E. and traces of medical science can be found in the "Rigveda" and "Atharvaveda". 2) The "Charaka(閣羅迦集)" and "$Su\acute{s}hruta$(妙聞集)", ancient texts from India, are not the work of one person, but the result of the work and errors of different doctors and philosophers. Due to the lack of historical records, the time of Charaka(閣羅迦) or $Su\acute{s}hruta$(妙聞)s' lives are not exactly known. So the completion of the "Charaka" is estimated at 1st$\sim$2nd century C. E. in northwestern India, and the "$Su\acute{s}hruta$" is estimated to have been completed in 3rd$\sim$4th century C. E. in central India. Also, the "Charaka" contains details on internal medicine, while the "$Su\acute{s}hruta$" contains more details on surgery by comparison. 3) '$V\bar{a}gbhata$', one of the revered Vriddha Trayi(triad of the ancients, 三醫聖) of the '$\bar{A}yurveda$', lived and worked in about the 7th century and wrote the "$Ast\bar{a}nga$ $Ast\bar{a}nga$ hrdaya $samhit\bar{a}$ $samhit\bar{a}$(八支集) and "$Ast\bar{a}nga$ Sangraha $samhit\bar{a}$(八心集)", where he tried to compromise and unify the "Charaka" and "$Su\acute{s}hruta$". The "$Ast\bar{a}nga$ Sangraha $samhit\bar{a}$" was translated into Tibetan and Arabic at about the 8th$\sim$9th century, and if we generalize the medicinal plants recorded in each the "Charaka", "$Su\acute{s}hruta$" and the "$Ast\bar{a}nga$ Sangraha $samhit\bar{a}$", there are 240, 370, 240 types each. 4) The 'Madhava' focused on one of the subjects of Indian medicine, '$Nid\bar{a}na$' ie meaning "the cause of diseases(病因論)", and in one of the copies found by Bower in 4th century C. E. we can see that it uses prescriptions from the "BuHaLaJi(布唅拉集)", "Charaka", "$Su\acute{s}hruta$". 5) According to the "Charaka", there were 8 branches of ancient medicine in India : treatment of the body(kayacikitsa), special surgery(salakya), removal of alien substances(salyapahartka), treatment of poison or mis-combined medicines(visagaravairodhikaprasamana), the study of ghosts(bhutavidya), pediatrics(kaumarabhrtya), perennial youth and long life(rasayana), and the strengthening of the essence of the body(vajikarana). 6) The '$\bar{A}yurveda$', which originated from ancient experience, was recorded in Sanskrit, which was a theorization of knowledge, and also was written in verses to make memorizing easy, and made medicine the exclusive possession of the Brahmin. The first annotations were 1060 for the "Charaka", 1200 for the "$Su\acute{s}hruta$", 1150 for the "$Ast\bar{a}nga$ Sangraha $samhit\bar{a}$", and 1100 for the "$Nid\bar{a}na$". The use of various mineral medicines in the "Charaka" or the use of mercury as internal medicine in the "$Ast\bar{a}nga$ Sangraha $samhit\bar{a}$", and the palpation of the pulse for diagnosing in the '$\bar{A}yurveda$' and 'XiZhang(西藏)' medicine are similar to TCM's pulse diagnostics. The coexistence with Arabian 'Unani' medicine, compromise with western medicine and the reactionism trend restored the '$\bar{A}yurveda$' today. 7) The "Charaka" is a book inclined to internal medicine that investigates the origin of human disease which used the dualism of the 'Samkhya', the natural philosophy of the 'Vaisesika' and the logic of the 'Nyaya' in medical theories, and its structure has 16 syllables per line, 2 lines per poem and is recorded in poetry and prose. Also, the "Charaka" can be summarized into the introduction, cause, judgement, body, sensory organs, treatment, pharmaceuticals, and end, and can be seen as a work that strongly reflects the moral code of Brahmin and Aryans. 8) In extracting bloody pus, the "Charaka" introduces a 'sharp tool' bloodletting treatment, while the "$Su\acute{s}hruta$" introduces many surgical methods such as the use of gourd dippers, horns, sucking the blood with leeches. Also the "$Su\acute{s}hruta$" has 19 chapters specializing in ophthalmology, and shows 76 types of eye diseases and their treatments. 9) Since anatomy did not develop in Indian medicine, the inner structure of the human body was not well known. The only exception is 'GuXiangXue(骨相學)' which developed from 'Atharvaveda' times and the "$Ast\bar{a}nga$ Sangraha $samhit\bar{a}$". In the "$Ast\bar{a}nga$ Sangraha $samhit\bar{a}$"'s 'ShenTiLun(身體論)' there is a thorough listing of the development of a child from pregnancy to birth. The '$\bar{A}yurveda$' is not just an ancient traditional medical system but is being called alternative medicine in the west because of its ability to supplement western medicine and, as its effects are being proved scientifically it is gaining attention worldwide. We would like to say that what we have researched is just a small fragment and a limited view, and would like to correct and supplement any insufficient parts through more research of new records.

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Fruiting body development and genetic analysis of somatic hybrids by protoplast fusion in edible fungi (식용버섯의 원형질체 융합체의 자실체 발생 및 유전분석)

  • Yoo, Young Bok;Kong, Won Sik;Oh, Se Jong;Jhune, Chang Sung;Shin, Pyung Gyun;Kim, Beom Gi;Kim, Gyu Hyun;Park, Minsun;Min, Byung Re
    • Journal of Mushroom
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    • v.2 no.3
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    • pp.115-126
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    • 2004
  • Somatic hybrids of inter-compatible and inter-incompatible strains were obtained by protoplast fusion. The fusion products between compatible strains, Pleurotus ostreatus and P. florida, formed heterokaryons, while fusants between incompatible strains such as P. cornucopiae + P. florida, P. ostreatus + Ganoderma applanatum, P. florida + Ganoderma lucidum, and P. ostreatus + Flammulina velutipes formed synkaryons that retained genes from both parents. The heterokaryons showed the same level of basidioma development. In contrast, the synkaryons showed unique characteristics including clamp connection formation at mitosis, either partner basidioma development, and abnormal segregation and recombination compared with inter-compatible strains. Synkaryons can be classified into homokaryoyic and heterokaryotic type. A comparison of somatic hybrids with compatible and incompatible strains was made using random amplified polymorphic DNA (RAPD) analysis. The heterokaryons between compatible species showed the same level of variability and contained both parental RAPD bands. In contrast, most of the synkaryons between incompatible species showed similarity to those of either parental bands and non-parental RAPD bands. Synkaryons can be classified into microgenome insertion type and macrogenome insertion type. A tetrapolar mating system was found among monospore isolates in somatic hybrids and wild type P. ostreatus. Homokaryons from each somatic hybrid combination were paired with tester homokaryons of the initial wild type of P. ostreatus. The changed mating types were identified in progenies. The pattern of mating type switching in somatic hybrids depends on compatibility of fusion partner. There are several factors related to the mechanism of clamp connection formation and fruiting body development of synkaryons. Of these,the major factor may be associated with self-fertility and mating type switching such as homokaryotic fruiting of wild type P. ostreatus. This review will discuss these aspects.

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