Korean Journal of Ecology and Environment (생태와환경)

The Korean Society of Limnology (한국수생태학회)
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Phylogenetic and Morphological Comparison between Thamnaconus septentrionalis and T. modestus Collected in Southwest Seashore

서남해에서 채집된 말쥐치 (Thamnaconus modestus)와 유사종 (T. septentrionalis)의 형태 및 계통유전학적 비교

  • Yu, Tae-Sik (Department of Fishery Science, Chonnam National University) ;
  • Park, Kiyun (Fisheries Science Institute, Chonnam National University) ;
  • Han, KyeongHo (Department of Fishery Science, Chonnam National University) ;
  • Kwak, Ihn-Sil (Fisheries Science Institute, Chonnam National University)
  • Received : 2021.09.15
  • Accepted : 2021.09.18
  • Published : 2021.09.30
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Abstract

Thamnaconus modestus, distributed in the Northwest Pacific, has high economic value and is used in various seafood. In this study, the morphological and genetic characteristics of T. modestus and T. septentrionalis were compared and analyzed. We observed the external and internal morphology of T. modestus, sketched skeletal elements, and analyzed phylogenetic evolutionary relationships using the cytochrome c oxidase subunit I (COI) gene on mitochondrial DNA compared to T. septentrionalis. The T. modestus observed in this study had blackish-brown patterns irregularly scattered on the gray-brown body, and the fins were blue-green. Genetic analysis results based on the COI sequences of T. modestus showed seven types of base sequence variation; however, the homology was more than 98.8%. In addition, as a result of comparison of the COI nucleotide sequences and phylogenetic analysis in Tetraodontiformes, two T. septentrionalis sequences (JN813099, MW485059) were similar to T. modestus with 99% homology, and the other two T. septentrionalis sequences (EF607583, KP267619) were similar to those of species belonging to another genus Thamnaconus with 95% homology with T. modestus. It was not easy to classify the species based on morphological characteristics, and phylogenetic analysis between T. modestus and T. septentrionalis confirmed the difference in classification. These results provide the external and internal morphology of T. modestus and will be used as important information for the taxonomic study of T. modestus and T. septentrionalis.

말쥐치는 북서태평양 해역에 분포하는 군집성 어류로 수산식품으로 이용되는 경제적 가치가 높은 어종이다. 본 연구에서는 서남해에 서식하는 국내산 말쥐치(Thamnaconus modestus)와 기존에 보고된 유사종(Thamnaconus septentrionalis)과의 형태적-유전적 특징을 비교-분석하였다. 이를 위해 서남해에서 채집한 말쥐치의 외부 형태와 골격을 관찰하고, 미토콘드리아 DNA의 cytochrome c oxidase subunit I (COI) 유전자 염기서열을 비교하여 유사종 (T. septentrionalis)과의 계통진화적 연관관계를 분석하였다. 서남해안 말쥐치의 외부 형태는 회갈색 바탕에 몸 전체에 흑갈색 무늬가 불규칙하게 산재된 패턴을 가지며 청록빛의 지느러미가 관찰되었다. 채집한 말쥐치에서 추출한 미토콘드리아DNA의 COI 유전자 분석결과 7종류의 염기서열 변이가 나타났으나 상동성이 전체적으로 98.8% 이상으로 유사성이 높게 나타났다. 또한 형태적으로 말쥐치 유사종인 T. septentrionalis COI 염기서열 비교와 계통유전학적 분석 결과, 보고된 시퀀스 중 상동성이 99%로 유전적으로 말쥐치로 보여지는 두 종(JN813099, MW485059)과 상동성 95%로 유사종으로 보이는 두 종(EF607583, KP267619)이 확인되었다. 말쥐치와 유사종은 계통유전적 분석결과 말쥐치와 유사종의 차이를 확인하였으나 형태적 특징으로 종 분류는 어려운 상태이다. 따라서 본 연구는 말쥐치의 외부 형태와 내부 골격 및 계통유전학적 정보를 제공함으로 말쥐치와 유사종(T. septentrionalis)의 분류학적 재검토를 진행하는 데 주요한 정보가 될 것이다.

Keywords

Acknowledgement

이 논문은 한국연구재단 중점연구사업의 지원을 받아 수행된 연구임[NRF-2018-R1A6A1A-03024314].

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