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STUDIES ON THE EXTRACTION OF SEAWEED PROTEINS 4. Precipitation Conditions and Nutritional Evaluation of Isolated Seaweed proteins (해조단백질 추출에 관한 연구 4. 추출단백질의 심전조건 및 영양적 평가)

  • WOO Soon-Im;RYU Hong-Soo;LEE Kang-Ho
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.12 no.4
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    • pp.225-234
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    • 1979
  • For the effective utilization of diverse and abundant resource of seaweeds in Korea as a food protein supplment, extraction conditions of water, salt, and alkali soluble proteins were investigated in previous work(Ryu and Lee, 1977: Lee et al., 1977: Lee et al., 1978). The present study as a part of the serial work was thus aimed to find the conditions of isolation and purification of extracted proteins, and to evaluate the nutritional quality of the isolated seaweed proteins in terms of amino acid composition, chemical score, protein score, modified essential amino acid index(MEAAI), and in vitro digestibility presented as pepsin-pancreatin digest residue index (PPDRI). As for the isolation of extracted proteins, TCA treatment was more effective for the proteins from rhodophyceae and Chlorophyceae while the precipitation at isoelectric point was more desirable for Phaeophyceae proteins. In amino acid composition, water soluble protein fraction was superior to the other fractions in Porphyra suborbiculata whereas both water and alkali soluble fractions seemed to bo more benefitial for Enteromorpha linza and Ulva pertusa; the extraction with alcohol-alkali mixed solvent for Undaria pinnatifida and Sargassum fulvellum. Glutamic acid and aspartic acid content was particularly high in all protein fractions examined. The total amino acid content of Porphyra suborbiculata and Enteromorpha linza was almost equivalent to that of dried whole egg although the essential amino acid content was lower. A comparative analysis was made on the inedexes between raw seaweed powder and isolated protein. Chemical score of Porphyra suborbiculata and Ulva pertusa was approximately 35 and 56 in cafes of raw powder and isolated protein respectively while only 10 to 16 for raw powder of Undaria pinnatifida and Sargassum fulvellum and 30 to 35 for their isolated proteins. Protein score of all isolated proteins was in the range of 63 to 73 which indicates that isolated protein would be mere valuable than the fern of raw seaweed powder. Digestibility by means of PPDRI was found to be extremely low in case of raw powder but it could be doubled in case of isolated protein yielding 67 to 70 for Porphyra suborbiculata and Ulva pertusa.

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The analysis of pesticide residue in leafy vegetables using the modified QuEChERS pre-treatment methods (QuEChERS 시료 처리법을 활용한 엽채류 중 잔류농약분석)

  • Kim, Yang-Hyeon;Hong, Su-Myeong;Son, Kyung-Ae;Lee, Ju-Young;Min, Zaw Win;Kwon, Hye-Young;Kim, Taek-Kyum;Kyung, Kee-Sung
    • The Korean Journal of Pesticide Science
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    • v.16 no.2
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    • pp.121-130
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    • 2012
  • In analyzing pesticide residue, LLE (liquid liquid extraction) is generally applied as one of the existing methods, but needed quite a lot of organic solvents and analytical apparatuses for the sample pre-treatment. In addition to its long analysis time and complex analytical processes, it is required to develop a more rapid and efficient method at present. In order to establish an economic and simple pesticide residue analytical method, this study carried out a comparative experiment on the existing analytical method with a new sample pre-treatment method named QuEChERS (quick, easy, cheap, effective, rugged and safe), which extracts and refines pesticide components by directly adding solid powder into the sample. Both the two analytical methods showed favorable values of correlation coefficient ($R^2$ > 0.99) of calibration curves. In terms of the detection limit (identification limit), imidacloprid showed 0.02 mg/kg, while the rest of pesticides showed a level around 0.05 mg/kg. The results of this experiment revealed that the recovery of LLE was 92.8-100.9% and the RSD was below 2.5%. On the other hand, the recovery of QuEChERS was 92.2-101.6% and RSD was below 1.9%. As a result of comparing the amount of pesticide residue by the time between the two analytical methods by using Paired t-Test, there was no significant difference between the two analytical methods as the p-value ranged from 0.3148-0.9890. Considering the results of the two methods, the QuEChERS method had similar recovery, compared to the analytical method using the existing LLE, and the analytical time was shortened by about one fourth of that of the existing method. Moreover, since it excludes the use of harmful organic solvents like dichloromethane during the process of extraction, thus leading to protecting experimenters health and remarkably reducing the amount of disused solvents, it is judged as an echo-friendly and economic analytical method.

Spaciotemporal Distributions of PM10 Concentration and Their Correlation with Local Temperature Changes : a Case Study of Busan Metropolitan City (PM10 농도의 시공간적 분포 특징과 국지적 기온 변화 간의 상관관계: 부산광역시 사례 분석)

  • Park, Sunyurp
    • Journal of the Korean association of regional geographers
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    • v.23 no.1
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    • pp.151-167
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    • 2017
  • The main objective of this study was to investigate the climatic impact of $PM_{10}$ concentration on the temperature change pattern in Busan Metropolitan City(BMC), Korea during 2001~2015. Mean $PM_{10}$ concentration of BMC has gradually declined over the past 15 years. While the highest $PM_{10}$ concentration was observed in spring followed by winter, summer, and fall on average, the seasonal variations of $PM_{10}$ concentration differed from place to place within the city. Frequency analysis showed that the most frequently observed $PM_{10}$ concentration ranged from $20{\mu}g/m^3$ to $60{\mu}g/m^3$, which accounted for 64.6% of all daily observations. Overall, the west-high and east-low pattern of $PM_{10}$ concentration was relatively strong during the winter when the effect of yellow-dust events on the air quality was weak. Comparative analyses between $PM_{10}$ concentration and monthly temperature slope derived from generalized temperature curves indicated that the decreasing trend of $PM_{10}$ concentration was associated with increases of annual temperature range, and $PM_{10}$ concentration had a negative relationship with the temperature slope of warming months. Overall, $PM_{10}$ concentration had a weak correlation with the annual mean temperature, but it had a significant, positive correlation with the winter season, which had a dominant influence on the annual mean temperature. In terms of energy budget, it has been known that the change in $PM_{10}$ concentration contributes to the warming or cooling effect by affecting the radiative forcing due to the reflection and absorption of radiant energy. The correlation between $PM_{10}$ concentration and temperature changes in the study area was not seasonally and spatially consistent, and its significance was statistically limited partly due to the number of observations and the lack of potential socioeconomic factors relevant to urban air quality.

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The Comparative Study on Health Promoting Behaviors by Shift Pattern of Duties of Women Workers in workplace (산업장 여성근로자의 근무형태에 따른 건강증진행위 비교)

  • Jang, Hee Jung;Park, Kyung Min
    • Korean Journal of Occupational Health Nursing
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    • v.8 no.1
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    • pp.22-41
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    • 1999
  • This study intended to analyze the health promoting behaviors, compare their factors according to the shift pattern of duties of women workers who were working in the industrial workplace and present basic data in planning systematic and effective programs of health promotion for three-shift system and day-duty. Using Quota Sampling, 219 women workers were selected as subjects from 5 workshops which had 50 settled workers up to 300 and 10 factories which had more than 300 located in Taegu and Kyung-Book Province. Data were collected by means of questionnaire from September 12 to September 30, 1998. As the instruments of the study were used Health Promotion Lifestyle Profile(HPLP) which was adapted and adjusted by Seo, Y. O. for health promoting behavior, the one developed by Moon, J. S. (1990) for health-belief, the one developed by Sherer et al.(1982) and then adapted by Oh, H. S. for self-efficacy, and the one developed by Park, J. W. (1985) for social support. The analysis of data were performed with Cronbach's ${\chi}^2$-test, t-test, ANCOVA, Kendal tau, Pearson correlation, Stepwise Multiple Regression test using SPSS program. The results of the study are as follows : 1. There was a significant difference in age(${\chi}^2=32.46$, p=0.000), career (${\chi}^2=18.47$, p=0.000), working day(t=-3.18, p=0.000) by the shift pattern of duties in terms of socio-demographic characteristics. 2. There was a statistically significant difference between the two groups on the health promoting behaviors (t=2,52, p=0.012). The score of three-shift group on health promoting behaviors was 2.27, showing that it was lower by .13 than that of day-duty group(2.40). 3. ANCOVA involving age, career and working day as covariables, which had revealed significant difference before, showed that health promoting behaviors by the shift patterns of duties was significantly different(F=4.88, p=0.028). 4. In consideration of variables that have an influence on health promoting behavior by the shift pattern of duties, social support occupied 19.4% of health promoting behavior in the three-shift group and 22.5% including the sense of self-efficacy. In the day-duty group, social support occupied 34.4% of health promoting behavior. 5. The score of three-shift group(2.94) was significantly lower than that of day-duty group(3.12) in the perceived benefit of health-belief(t= -3.29, p=0.001), while the score of three-shift group (2.48) was significantly higher than that of day-duty group(2.24) in the perceived barrier (t=4.22, p=0.000). In the sense of self-efficacy(t=-4.20, p=0.000), the score of three-shift group(3.24) was significantly lower than that of day-duty group(3.53) while in social support(t=-4.56, p=0.000) the one of three-shift group(2.64) was significantly lower than that of day-duty group(2.88). The suggestions are as follows on the basis of the results of this study : 1. It is required to develop health promoting program that takes the shift pattern of duties of women workers into consideration. In addition, there are special demands on developing nursing strategies for health promoting behavior of three-shift workers. 2. It is required to develop specific strategies for social support which is the most significant factor to the health promoting behavior for women workers. 3. It is necessary to develop some programs for improving the sense of self-efficacy, social support, and health-belief of three-shift workers. To achieve these tasks, industrial nurses should play an active role and improve the ability of self-health care of women workers.

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A Comparative Study of Landscape Characteristics on Bridges in Palaces of Korea and China - Focusing on the Chosun Dynasty and Ming and Qing Dynasties - (한국과 중국의 궁궐 내 교량에 관한 경관특성 비교 연구 - 조선시대와 명·청시대를 중심으로 -)

  • Zhang, Fu-Chen;Lee, Ai-Ran
    • Journal of the Korean Institute of Traditional Landscape Architecture
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    • v.37 no.3
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    • pp.1-12
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    • 2019
  • A bridge is a structure constructed on water or in the air for convenient passage. Compared to other buildings, the building materials and structures of bridge required unique functions to cross the space. It depends on the productivity of the building, the level of science and technology, and the ecological environment of the building site. Also, it has important relationship with functions such as politics, military, economy, and life. Most of the academic research on bridges is focused on research in the field of bridge-building technology, so the study on the landscape aesthetics and history of bridges is lacking. Against this backdrop, the study will be valuable as a accumulation of both countries' understanding of bridge types, history and culture, as well as technical and aesthetic data, by analyzing the bridges located within the palaces of Korea and China. The research method is to analyze the bridge through field survey and literature analysis.. First, the bridges of royal palace of Korea and China are to be classified quantitatively as physical shapes, landscapes, and decorations by comparing the materials, forms, landscapes, and decorative culture of bridges. Second, characteristics, common points, and differences are extracted by classifying bridges of both countries. Also, the results are discussed based on the physical environment or cultural background. This would be worth cross-referencing in the building technology and aesthetics of the two countries. For the first important characteristics of result, main materials of Korean and Chinese palaces are stone. However, the bridge in China's royal palaces is also focused on wood. Second, in terms of form, the bridges in the royal gardens of Korea and China are all based on the beam bridge. However, the specific form, ratio, style of the beam bridge, and airspace of arched bridge are very different. Third, most of the connection methods are focused on the over bridge. It values the convergence with the surrounding landscape. Due to the difference in the area and location of water, the bridge in the Korean palace is more focused on the convergence of the surrounding buildings and plants, while the bridge in the Chinese palace is more concerned about the harmony of hydration. Fourth, the decoration places importance on the artistry and aesthetics of both the bridges in Korea and China. There is a difference in style in the same type of decoration due to culture.

A Comparative Analysis of News Frame on U. S. Beef Imports and Candlelight Vigils (미국산 수입쇠고기와 촛불시위 보도에 나타난 뉴스 프레임 비교 연구)

  • Im, Yang-June
    • Korean journal of communication and information
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    • v.46
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    • pp.108-147
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    • 2009
  • This study explores the news frames on the U. S. beef imports and candlelight vigils covered by the two national dailies such as ChosunIlbo and the Hankyoreh Shinmun; the KwangwonIlbo, a local daily. The news frames extracted based on the models of Iyengar(1987), Semetko & Valkenburg(2000) and other researchers are attribution of responsibility, economic sequences, protest against the authorities, national health and governmental public relations and so on. The result shows that the news reports are consisted of the straight news(75.9%), feature stories(11.7%) and editorials(6.3%). More specifically, there is a comparatively hight ratio of editorials(11.0%) for the ChosunIlbo, feature stories(20.9%) for the Hankyoreh, and the straight news(89.7%) for the KwangwonIlbo. In terms of the news frames stressed by the three dailies, the ChosunIlbo focuses and stresses on the national health(17.8%) and the attribution of responsibilities(10.6%). However, the Hankyoreh have a tendency to stress on the protest against the authorities(31.3%) and attribution of responsibilities(38.4%); the KwangwonIlbo, focuses on the protest against the authorities(38.4%) and the economic sequences(17.9%). Finally, in the case of the main characteristics of the dailies, the governmental public relations frame is found only on the ChosunIlbo that has a comparatively high ratio; the Hankyoreh also has a high ratio of the feature stories on the U. S. beef imports. Even thought the KwangwonIlbo has a high ratio of the economic sequence frame, the ratio of opinion pages, such as editorial and columns, the local newspaper has not spoken up for the potential economic crisis of the local Kwangwon province beef industry, mainly caused by the U. S. import beef.

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A Basic Study on the Establishment of Evaluation Items for the Resiliency of Planting Landscape in Hahoe and Yangdong of World Cultural Heritage (세계문화유산 하회와 양동의 식생경관 진정성 유지를 위한 평가항목 설정 기초 연구)

  • Lee, Chang-Hun;Shin, Hyun-Sil
    • Journal of the Korean Institute of Traditional Landscape Architecture
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    • v.36 no.2
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    • pp.21-29
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    • 2018
  • This study was wanted to select a value evaluation item to maintain the authenticity of the Planting Landscape in Hahoe and Yangdong villages. Above all, after checking the suitability of the 43 selected items by the Focus Group Interview, the weight was calculated in the Analytic Hierarchy Process expert questionnaire to establish the importance of the indicators for the development of the assessment items. The expert analysis analyzed the importance of 2 sections, 6 divisions and 11 detailed categories, and summarized the results of the study as follows : First, the comparative importance of each category and selection of the assessment items for the stabilization of the Planting Landscape of Hahoe and Yangdong indicated that cultural values are more important than biological values. In particular, details of the biological values of trees were derived with relatively low estimates, except for the specific characteristics of species and items of type of tree. Second, as a result of verification of the suitability of the 43 items selected by the Focus Group Interview, the source diameter, the collection width, reception, flushing, supersonality, records, memorials, 11 items were selected, Third, the results of the importance evaluation of the value properties for maintaining the resistance of vegetation through the Analytic Hierarchy Process were : specific in biological values (0.187), steady (0.094), and water pipe width (2007). There was relatively little difference in the highest weights, the width of the pipe except for the lowest value received, and the source diameter flushing. Fourth, the results of an evaluation of the importance of a value property aimed at historical values were whether or not the cultural asset was designated (0.134), the record value (0.092), the time (0.088), and the monument (0.063). In terms of the importance of evaluating the historical values of Planting sites consisting of Hahoe and Yangdong, the importance of designation of cultural properties was considered to be relative to the maintenance of the Planting Landscape including culture and history. Based on the assessment items and weighted values of the Planting Landscape of Yangdong Village and the World Cultural Heritage below and below, this study's Analytic Hierarchy Process can be applied to actual criteria for the assessment of the authenticity of trees in the village. Based on the assessment items and weighted values of the Planting Landscape in Hahoe and Yangdong villages, a follow-up study on the assessment standards for the authenticity of trees in the village will be left as a future task.

Predictive Clustering-based Collaborative Filtering Technique for Performance-Stability of Recommendation System (추천 시스템의 성능 안정성을 위한 예측적 군집화 기반 협업 필터링 기법)

  • Lee, O-Joun;You, Eun-Soon
    • Journal of Intelligence and Information Systems
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    • v.21 no.1
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    • pp.119-142
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    • 2015
  • With the explosive growth in the volume of information, Internet users are experiencing considerable difficulties in obtaining necessary information online. Against this backdrop, ever-greater importance is being placed on a recommender system that provides information catered to user preferences and tastes in an attempt to address issues associated with information overload. To this end, a number of techniques have been proposed, including content-based filtering (CBF), demographic filtering (DF) and collaborative filtering (CF). Among them, CBF and DF require external information and thus cannot be applied to a variety of domains. CF, on the other hand, is widely used since it is relatively free from the domain constraint. The CF technique is broadly classified into memory-based CF, model-based CF and hybrid CF. Model-based CF addresses the drawbacks of CF by considering the Bayesian model, clustering model or dependency network model. This filtering technique not only improves the sparsity and scalability issues but also boosts predictive performance. However, it involves expensive model-building and results in a tradeoff between performance and scalability. Such tradeoff is attributed to reduced coverage, which is a type of sparsity issues. In addition, expensive model-building may lead to performance instability since changes in the domain environment cannot be immediately incorporated into the model due to high costs involved. Cumulative changes in the domain environment that have failed to be reflected eventually undermine system performance. This study incorporates the Markov model of transition probabilities and the concept of fuzzy clustering with CBCF to propose predictive clustering-based CF (PCCF) that solves the issues of reduced coverage and of unstable performance. The method improves performance instability by tracking the changes in user preferences and bridging the gap between the static model and dynamic users. Furthermore, the issue of reduced coverage also improves by expanding the coverage based on transition probabilities and clustering probabilities. The proposed method consists of four processes. First, user preferences are normalized in preference clustering. Second, changes in user preferences are detected from review score entries during preference transition detection. Third, user propensities are normalized using patterns of changes (propensities) in user preferences in propensity clustering. Lastly, the preference prediction model is developed to predict user preferences for items during preference prediction. The proposed method has been validated by testing the robustness of performance instability and scalability-performance tradeoff. The initial test compared and analyzed the performance of individual recommender systems each enabled by IBCF, CBCF, ICFEC and PCCF under an environment where data sparsity had been minimized. The following test adjusted the optimal number of clusters in CBCF, ICFEC and PCCF for a comparative analysis of subsequent changes in the system performance. The test results revealed that the suggested method produced insignificant improvement in performance in comparison with the existing techniques. In addition, it failed to achieve significant improvement in the standard deviation that indicates the degree of data fluctuation. Notwithstanding, it resulted in marked improvement over the existing techniques in terms of range that indicates the level of performance fluctuation. The level of performance fluctuation before and after the model generation improved by 51.31% in the initial test. Then in the following test, there has been 36.05% improvement in the level of performance fluctuation driven by the changes in the number of clusters. This signifies that the proposed method, despite the slight performance improvement, clearly offers better performance stability compared to the existing techniques. Further research on this study will be directed toward enhancing the recommendation performance that failed to demonstrate significant improvement over the existing techniques. The future research will consider the introduction of a high-dimensional parameter-free clustering algorithm or deep learning-based model in order to improve performance in recommendations.

Predicting link of R&D network to stimulate collaboration among education, industry, and research (산학연 협업 활성화를 위한 R&D 네트워크 연결 예측 연구)

  • Park, Mi-yeon;Lee, Sangheon;Jin, Guocheng;Shen, Hongme;Kim, Wooju
    • Journal of Intelligence and Information Systems
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    • v.21 no.3
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    • pp.37-52
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    • 2015
  • The recent global trends display expansion and growing solidity in both cooperative collaboration between industry, education, and research and R&D network systems. A greater support for the network and cooperative research sector would open greater possibilities for the evolution of new scholar and industrial fields and the development of new theories evoked from synergized educational research. Similarly, the national need for a strategy that can most efficiently and effectively support R&D network that are established through the government's R&D project research is on the rise. Despite the growing urgency, due to the habitual dependency on simple individual personal information data regarding R&D industry participants and generalized statistical data references, the policies concerning network system are disappointing and inadequate. Accordingly, analyses of the relationships involved for each subject who is participating in the R&D industry was conducted and on the foundation of an educational-industrial-research network system, possible changes within and of the network that may arise were predicted. To predict the R&D network transitions, Common Neighbor and Jaccard's Coefficient models were designated as the basic foundational models, upon which a new prediction model was proposed to address the limitations of the two aforementioned former models and to increase the accuracy of Link Prediction, with which a comparative analysis was made between the two models. Through the effective predictions regarding R&D network changes and transitions, such study result serves as a stepping-stone for an establishment of a prospective strategy that supports a desirable educational-industrial-research network and proposes a measure to promote the national policy to one that can effectively and efficiently sponsor integrated R&D industries. Though both weighted applications of Common Neighbor and Jaccard's Coefficient models provided positive outcomes, improved accuracy was comparatively more prevalent in the weighted Common Neighbor. An un-weighted Common Neighbor model predicted 650 out of 4,136 whereas a weighted Common Neighbor model predicted 50 more results at a total of 700 predictions. While the Jaccard's model demonstrated slight performance improvements in numeric terms, the differences were found to be insignificant.

The taxonomic implication of leaf micromorphological characteristics in the genus Aruncus (Rosaceae) (눈개승마속(장미과) 잎 표피 미세형태학적 형질 및 분류학적 유용성)

  • OAK, Min-Kyeong;SONG, Jun-Ho;HONG, Suk-Pyo
    • Korean Journal of Plant Taxonomy
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    • v.48 no.2
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    • pp.143-152
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    • 2018
  • A comparative study of leaf epidermal microstructures in genus Aruncus (two species, five varieties) was carried out using scanning electron microscopy in order to evaluate their significance in terms of taxonomy. All of the leaves of the taxa studied here were amphistomatic with undulate anticlinal walls, and smooth and flat periclinal walls on both surfaces. The size range of the stomata complex is $8.95-21.97{\times}7.50-16.99{\mu}m$: the largest one was found in Aruncus dioicus var. astilboides (average $18.01{\times}13.47{\mu}m$) and the smallest was measured and determined to be A. gombalanus (average $11.11{\times}8.94{\mu}m$). An anomocytic stomata complex was found in all of the studied taxa. The stomatal frequency on average was $27.54/0.05mm^2$; it is highest in A. gombalanus ($60.4/0.05mm^2$) and lowest in A. dioicus var. acuminatus ($11.6/0.05mm^2$). Two types (short stalked capitate glandular trichome and non-glandular trichome) of trichomes are found in the leaves. The non-glandular trichome was divided into three types based on the presence and degree of development of subsidiary cells. Anomocytic stomata of the hypostomatic type and the distribution pattern of capitate glandular trichomes were the major characters in this genus. The stomata size and frequency, the epidermal cell structure, the trichome type and the distribution pattern may have diagnostic importance among the taxa in the genus. Our leaf micromorphological results provide useful information for the taxonomic revision of the genus Aruncus.