• Animal cells and systems
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• 제8권1호
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• pp.49-55
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• 2004

In vertebrates, multisubunit cofactors regulate gene expression through interacting with cell-type- and gene-specific DNA-binding proteins in a chromatin-selective manner. ADD1/SREBP1c regulates fatty acid metabolism and insulin-dependent gene expression through binding to SRE and E-box motif with dual DNA binding specificity. Although its transcriptional and post-translational regulation has been extensively studied, its regulation by interacting proteins is not well understood. To identify cellular proteins that associate with nuclear form of ADD1/SEBP1c, we employed the GST pull-down system with Hela cell nuclei extract. In this study, we demonstrated that Ku proteins interact specifically with ADD1/SREP1c protein. GST pull-down combined with peptide sequencing analysis revealed that Ku80 binds to ADD1/SREBP1c in vitro. Additionally, western blot analysis showed that Ku70, a heterodimerizing partner of Ku80, also associates with ADD1/SREBP1c. Furthermore, co-transfection of Ku70/Ku80 with ADD1/SREBP1c enhanced the transcriptional activity of ADD1/SREBP1c. Taken together, these results suggest that the Ku proteins might be involved in the lipogenic and/or adipogenic gene expression through interacting with ADD1/SREBP1c.

• 한국미생물·생명공학회지
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• 제41권1호
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• pp.128-134
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• 2013

Salmonella enterica serovar typhi, a Gram-negative food-borne pathogen, causes typhoid fever in humans. OmpC is an outer membrane porin of S. typhi expressed throughout the infection period. OmpC is potentially an attractive antigen for multivalent vaccines and diagnostic kit designs. In this study we combined in silico, in vitro and in vivo approaches to analyze various aspects of OmpC's antigenic properties. The conserved region, in addition to secondary and tertiary structures, and linear B cell epitopes, were predicted. A number of results obtained from in silico analyses were validated by experimental studies. OmpC was amplified, cloned and then expressed, with the recombinant protein then being purified. BALB/c mice were immunized by purified denatured OmpC. The titer of antibody was raised. Results of challenges with the pathogen revealed that the immunity is non-protective. Most of the theoretical and experimental results were in consensus. Introduced linear B cell epitopes can be employed for the design of diagnostic kits based on antigen-antibody interactions.

• 대한수의학회지
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• 제39권4호
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• pp.743-750
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• 1999

Bovine viral diarrhea viruses (BVDVs) were isolated from cattle with respiratory and diarrhea signs as well as persistently infected cattle. These isolates were analysed serologically to characterize serogroups and to compare serological relationship with reference viruses of type I and II. Most isolates from calf diarrheal cases and persistently infected individuals showed a significant difference in cross-neutralization test with the viruses isolated from nasal discharges showing severe respiratory signs. Serologically most of the commercial vaccine strains could be classified into classical BVDV (type I) such as NADL strain. This serological difference among BVDV isolates suggested the need for new vaccines to protect cattle from both respiratory and enteric BVDV infections in field. The immunogenicity of BVDVs which showed a good propagation capability in MDBK cells and high rates of neutralizing activity (isolate : KD26-1, PHG, B5 and 95002) against all viruses used in this study, was confirmed in guinea pig when treated as single or combined groups.

• IMMUNE NETWORK
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• 제15권1호
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• pp.9-15
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• 2015

Streptococcus pneumoniae (the pneumococcus) causes a major upper respiratory tract infection often leading to severe illness and death in the elderly. Thus, it is important to induce safe and effective mucosal immunity against this pathogen in order to prevent pnuemocaccal infection. However, this is a very difficult task to elicit protective mucosal IgA antibody responses in older individuals. A combind nasal adjuvant consisting of a plasmid encoding the Flt3 ligand cDNA (pFL) and CpG oligonucleotide (CpG ODN) successfully enhanced S. pneumoniae-specific mucosal immunity in aged mice. In particular, a pneumococcal surface protein A-based nasal vaccine given with pFL and CpG ODN induced complete protection from S. pneumoniae infection. These results show that nasal delivery of a combined DNA adjuvant offers an attractive potential for protection against the pneumococcus in the elderly.

• 생약학회지
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• 제20권3호
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• pp.196-203
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• 1989

Experimental studies were conducted to investigate the effect of Sagan-Tang on analgesic, sedative, antipyretic, isolated ileum and blood vessel and so on. The results of this investigation were summarized as follows; Analgesic action by the acetic acid stimulating method in mice were recognized. Prolonging action against the hypnotic duration induced by thiopental-Na was noted in mice. Antipyretic effect in typhoid vaccine febrile rats was recognized. Spontaneous motility of the isolated ileum of mice was suppressed and contractions of the isolated ileum of mice and guinea-pig induced by accetylcholine chloride, barium chloride and histamine were remarkably inhibited. Vaso-diating and hypotensive actions were recognized in rabbits. GOT and GPT activities in the serum of rats damaged by $CCl_4$ and galactosamine were decreased remarkably.

• Journal of Acupuncture Research
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• 제39권2호
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• pp.139-144
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• 2022

Peripheral facial nerve palsy is a neurological condition/disease characterized by unilateral paralysis of the facial muscles. We report the effects of complex Korean medicine treatment in 2 patients diagnosed with Bell's palsy who developed symptoms 3-4 days following vaccination with ChAdOx1 nCoV-19 and who received oral steroids and antiviral medications. Complex Korean medicine treatments including acupuncture, electroacupuncture, bee venom acupuncture, cupping therapy, herbal medicine, and physical therapy were performed. Symptom improvement was evaluated daily using the Numerical Rating Scale, House-Brackmann Grade, and Yanagihara's Score. Patient 1 was hospitalized for 22 days and Patient 2 visited the outpatient clinic 20 times. In both cases, postauricular pain caused by Bell's palsy disappeared, and symptoms of facial paralysis improved following completion of treatment. These findings could suggest that combined Korean medicine treatment may improve symptoms of peripheral facial nerve palsy.

• 한국가금학회:학술대회논문집
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• 한국가금학회 2004년도 제21차 정기총회 및 학술발표회
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• pp.23-25
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• 2004

본 실험은 감태와 crude lectin의 사료내 첨가가육계의 생산성 및 면역반응에 미치는 영향을 조사하기 위하여 실시하였다. 1일령 Ross 수평아리 총234수를 공시하여 대조구(-), 대조구(+), 감태 1.0%, crude lectin 0.05 %, 0.1 %, 및 0.3 %로 6처리 3반복, 반복당 13수씩을 총 38일간 실험사료를 급여하였고, ND-IB 혼합 사독백신은 4일령에 피하접종하였다. 사료요구율에 있어서는 crude lectin 0.3 % 첨가구가 대조구(-)에 비해 유의하게 낮게 나타났다(P<0.05). ND-IB 사독 혼합백신 접종 3주 후에는 감태와 crude lectin의 첨가구의 ND와 IB 백신 역가가 대조구(+)와 비교하여 상승하는 경향이나 상승효과가 있었다(P<0.05). 폐사율에 있어서 crude lectin 첨가구들은 살모넬라를 감염시킨 대조구(+)에 비해 유의하게 감소하였다(P<0.05). 닭에서 IFN-v, IL-2, 및 IL-6의 mRNA는 살모넬라 감염에 의해 높게 발현되었고, 감태와 crude lectin은 IFN-v의 발현에 영향을 미치지 않았으나, 감태 1.0 %와 crude lectin 0.05 % 첨가구는 IL-2와 IL-6의 mRNA 농도가 대조구(+)에 비해 높은 경향이나 유의하게 높았다(p<0.05).

• 미생물학회지
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• 제41권4호
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• pp.269-274
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• 2005

동물용 생 바이러스 백신 내에 mycoplasma를 검출하기 위해 polymerase chain reaction (PCR)기법과 2가지의 상품화된 PCR 검출킷트를 평가하였다. PCR기법은 시험에 사용된 모든 mycoplasma를 특이적으로 검출할 수 있었으나, 2가지의 상품화된 PCR 검출킷트는 일부의 mycoplasma를 검출하지 못하였다. 또한, PCR기법의 검출 특이도는 조류 유래 mycoplasma에 속한 4주의 표준주 및 7주의 야외분리주를 모두 검출할 수 있었다. PCR기법의 민감도는 9 CFR Mycoplasma액체배지에서 배양한 Mycoplasma 속균 및 Acholeplasma속균에 대해 $1\~100$ colony forming units/ml까지 검출할 수 있었다. 동물용생 바이러스 백신에 대해 PCR기법의 적용가능성을 평가하기 위해, 돼지 전염성위장염 및 로타바이러스 흔합백신과 개 파보바이러스 백신내에 A. laidlawii를 인공적으로 접종한 후, PCR기법의 민감도를 조사하였을 때 배양액을 이용한 검출한계와 유사하였다. 본 연구에서 사용된 PCR 기법은 동물용 생 바이러스 백신내의 mycoplasma를 신속하고 민감하게 검출할 수 있을 것으로 판단되었다.

• Pediatric Infection and Vaccine
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• 제8권2호
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• pp.222-228
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• 2001

아직도 우리나라에서는 미국과 같은 선진국과 비교해 폐결핵의 유병률이 높은 상태이다. 그리고 높은 폐결핵 발생과 함께 장결핵의 발생도 높을 것으로 예상되나 정확한 통계 및 연구는 미비한 실정이다. 또한, 발생 가능성이 높을 것으로 예상되는 장결핵에 의한 영양실조에 대한 관심도 부족한 실정이다. 저자 등은 결핵 발생이 현격하게 감소되지 않는 한 앞으로도 계속 발생할 수 있는 장결핵에 의한 영양실조의 가능성을 상기하고자 장결핵에 의한 영양실조 1례를 경험하였기에 문헌 고찰과 함께 보고하는 바이다.

• Pediatric Infection and Vaccine
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• 제27권2호
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• pp.127-133
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• 2020

Parvimonas micra는 아포 비형성 혐기성 그람 양성 알균으로, 피부, 잇몸, 질, 위장관 등에 정상 상재균으로 존재하며 주로 치과 치료 등의 침습적 치료 후 기회 감염을 일으킬 수 있다. 저자들은 최근 1년 이내 치과 질환을 포함한 특이 과거력이 없는 11세 소아에서 P. micra에 의해 발생한 뇌농양을 경험하여 보고하고자 한다. 환자는 내원 7일 전부터 두통, 구토가 발생하였으며, 내원일 복시가 발생하였다. 뇌 자기공명영상에서 좌측 전두정엽 뇌농양으로 진단한 후 수술적 배농을 시행하였으며, 수술 시 흡인한 검체의 혐기성 세균 배양 검사에서 P. micra가 동정되었다. 수술 후 6주간 ceftriaxone과 metronidazole 항생제 병합요법으로 치료한 후 재발 없이 안정된 상태를 보이고 있다.