• Korean Journal of Pharmacognosy
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• v.16 no.2
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• pp.73-80
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• 1985

Experimental studies were undertaken to investigate for the effect of Yijin-tang on the digestive system and isolated uterus of rats. Yijin-tang was composed of five crude drugs; Pinelliae Tuber, Aurantii nobilis Pericarpium, Hoelen, Glycyrrhizae Radix and Zingiberis Rhizoma. The results obtained from experiments with water soluble fraction extracted from Yijin-tang were as follows; Spontaneous motilities of isolated ileum of mice and rabbits were suppressed, and contraction of isolated ileum of mice and guinea-pigs induced acetylcholine, barium chloride and histamine were inhibited. The anticathartic action of mice induced by castor oil were significantly observed. The spontaneous motility of isolated uterus of rats was suppressed. Inhibitory effect of gastric juice secretion and antiulcerogenic action were significantly shown in Shay rats.

• Korean Journal of Pharmacognosy
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• v.16 no.1
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• pp.18-25
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• 1985

Experimental studies were conducted to investigate for the effect of Tongkyu-tang on analgesic, antipyretic, sedative, anti-inflammatory, isolated ileum and anti-histamine actions. Tongkyu-Tang was composed of sixteen crude drugs including Astragali Radix, which has been used for the treatment of common cold, headache, rhinitis, nasal obstruction, rhincospasm, etc. The following results have been obtained; analgesic and antipyretic actions were obtained. Prolonged action against the hypnotic duration of pentobarbital were significantly noted in mice. Anti-inflammatory effects in the paw edema induced by 1% carrageenin and 1% dextran were significantly shown in rats. Spontaneous motilities of isolated ileum of mice were strongly suppressed, and contractions of isolated ileum of mice and guinea-pigs induced by acetylcholine chloride, barium chloride and histamine were remarkably inhibited. Antihistamine actions were noted. Considering the above experimental results, it is suggested that effects of Tongkyu-tang based on the Oriental Medical References were similarly consistent with the actual experimental results.

• Journal of Pharmaceutical Investigation
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• v.15 no.2
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• pp.63-72
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• 1985

Analgesic, anticonvulsive, sedative and antipyretic actions of Yangkyuksanwhatang which is composed of nine crude drugs including Rehmanniae Radix, and its effects on isolated ileum and blood vessel were investigated. The results of the studies were summerized as follows; 1. Analgesic and antipyretic effects were observed. 2. Suppressive actions were not shown on the convulsion induced by strychnine, but significant effects were noted on the convulsion induced by picrotoxin. 3. A prolongation of anesthetic time induced by pentobarbital sodium was significantly observed. 4. Relaxing actions were noted on the ileum of mice, and also, same effects were recognized on the contraction of the ileum due to acetylcholine chloride and barium chloride. 5. The expansion of blood vessels by relaxation of smooth muscle and hypotensive effects were noted.

• Korean Journal of Pharmacognosy
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• v.20 no.3
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• pp.188-195
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• 1989

Hyangsayangwee-Tang, a combined preparation of crude drugs, which has been used for stomach and intestinal disorder, was examined for anti-spasmodic, anti-ulcerative and anti-emetive effects. Spontaneous motility of isolated ileum was strongly suppressed and inhibitory effects against contraction of isolated ileum induced by acetylcholine and barium chloride were shown in mice. And, contraction of isolated guinea-pig ileum by histamine was inhibited. In rats fundus preparations, Hyangsayangwee-Tang elicited strong relaxation and had antagonist effects against the spasm induced by acetylcholine and barium chloride. A significant inhibitory effect on the intestinal propulsion of barium sulfate in mice was shown. In pylorus-ligated rats, Hyangsangwee-Tang inhibited gastric secretion and showed a strong anti-peptic activity. Protective effects against gastric ulceration induced by pyloric ligation, water-immersion stress, histamine and aspirin were significantly recognized in mice and rats. Hyangsayangwee-Tang decreased cupric sulfate-induced vomitting in frogs.

• Journal of Physiology & Pathology in Korean Medicine
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• v.27 no.6
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• pp.759-763
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• 2013

This experiment was performed to investigate whether 50% ethanol extracts of the combined-preparation of Longanae Arilus, Chrysanthemi Flos, Zizyphi Fructus and Ginseng Radix alba (CPE) has hypnotic effects and/or enhances pentobarbital-induced sleeping time. Locomotor activity was evaluated using a ambulometer of tilting-type. The sedative-hypnotic effects were evaluated by measuring the sleeping onset time and sleeping time in pentobarbital-treated mice 30 min. after oral administration of CPE and muscimol. The intracellular $Cl^-$ concentration of cerebellar granule cells was estimated using $Cl^-$ sensitive fluorescence probe N-(ethoxycarbonylmethyl)-6-methoxyquinolinium (MQAE). CPE (150 mg/kg) decreased the locomotor activity, but CPE itself did not induce sleep. However, CPE reduced sleeping onset and prolonged sleeping time induced by pentobarbital (42 mg/kg). In addition, CPE (2 ${\mu}g/ml$) and pentobarbital (2.5 ${\mu}M$) itself did not affect on the chloride influx in primary cultured cerebellar granule cells, but the combination of CPE and pentobarbital (2.5 ${\mu}M$) increased the chloride influx onto the cells. In conclusion, it is suggested that CPE might augment pentobarbital-induced sleep through the increase of chloride influx.

• Korean Journal of Pharmacognosy
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• v.18 no.4
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• pp.241-248
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• 1987

Woohwangchungsim-Won has been widely used for the treatment of appoplexy, hypertension, arteriosclerosis, insomnia and cerebrovascular accident, etc in oriental hospital and pharmacy. In order to investigate the efficacy of Woohwangchungsim-Won, the water extract of it were bioassayed for isolated ileum, blood vessels, blood pressure, heart and diuresis. The results of this studies were as follows; Spontaneous motilities of isolated ileum of mice were strongly suppressed, and contraction of isolated ileum of mice and guinea-pigs induced by acetylcholine chloride, barium chloride and histamine were inhibited. Vaso-dilating action due to vascular smooth muscle relaxation in frogs and rabbits, and hypotensive action in anesthetized rabbits were noted. Negative inotropic action on the isolated frog heart and diuretic effect in rabbits were shown.

• Korean Journal of Pharmacognosy
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• v.19 no.1
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• pp.39-46
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• 1988

Experimental studies were conducted to investigate the hemostatic effect of the water extracts of Kwibitang. For this purpose, the effects of the extracts on the bleeding time in mouse tail and prothrombin time in vitro were estimated. Furthermore, its activity on the isolated uterine muscle in rats were investigated. The results obtained were as following; The bleeding time was not shortened, but the plasma prothrombin time in vitro test was significantly shortened. The uterotonic action produced by the extract was not inhibited by pretreatment of diltiazem at the doses of $10^{-5}g/ml$.

• Korean Journal of Pharmacognosy
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• v.13 no.4
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• pp.157-162
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• 1982

This study was undertaken to investigate effects of 'Oyo-Tang' on the respiratory system. The results of this study were summarized as follows; 1. Antihistamine and relaxed dialating actions were recognized on the extracted ileum and serial broncheal samples in guinea-pigs. 2. Mild hypertensive action was recognized on the carotid artery in rabbits. 3. Antitussive action was noted on mechanically irritated coughs in dogs and cats. In connection with the results of these studies, effects based on the Oriental medical references were consistent with the actual experimental results. It was considered that effective application of 'Oyo-Tang' for the treatment of cough and asthma could be justified.

• Korean Journal of Pharmacognosy
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• v.19 no.1
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• pp.47-52
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• 1988

In an attempt to investigate the effect of Beekeumsan on the hemostatic action and isolated uterine muscle, Beekeumsan was administered orally and the bleeding time in mouse tail, prothrombin time in vitro were estimated. Its activity on the isolated uterine muscle in rats were investigated. The following results were obtained; The bleeding time was not shortened, but the plasma prothrombin time in vitro was significantly shortened. The uterotonic action produced by the Beekeumsan was not inhibited by pretreatment of atropine, but was slightly inhibited by cyproheptadine and completely inhibited by pretreatment of diltiazem.

• Korean Journal of Pharmacognosy
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• v.14 no.1
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• pp.9-16
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• 1983

Experimental studies were implemented to evaluate clinical efficacy of 'Daeshiho-Tang' and to validate Oriental medicine description of the recipe which has been widely used in metabolic diseases and febrile diseases. Following results were obtained; 1. Suppressive action was shown on convulsion due to cerebrocortical causes but no such action was noted either myelic or diencephatic causes in mice. 2. Analgesic and antipyretic actions were recognized in mice, rats and rabbits. 3. Antispasmodic actions were recognized on contraction of the ileum induced by $BaCl_2$ and histamine in mice and guinea-pigs. 4. Transport accelerating action of the small intestine was gained in mice.