Browse > Article

Effects of the Combined-Preparation of Crude Drugs on Pentobarbital-induced Sleeping Time  

Han, Young Taek (College of Pharmacy, Woosuk University)
Kim, Dae Keun (College of Pharmacy, Woosuk University)
Eun, Jae Soon (College of Pharmacy, Woosuk University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.27, no.6, 2013 , pp. 759-763 More about this Journal
Abstract
This experiment was performed to investigate whether 50% ethanol extracts of the combined-preparation of Longanae Arilus, Chrysanthemi Flos, Zizyphi Fructus and Ginseng Radix alba (CPE) has hypnotic effects and/or enhances pentobarbital-induced sleeping time. Locomotor activity was evaluated using a ambulometer of tilting-type. The sedative-hypnotic effects were evaluated by measuring the sleeping onset time and sleeping time in pentobarbital-treated mice 30 min. after oral administration of CPE and muscimol. The intracellular $Cl^-$ concentration of cerebellar granule cells was estimated using $Cl^-$ sensitive fluorescence probe N-(ethoxycarbonylmethyl)-6-methoxyquinolinium (MQAE). CPE (150 mg/kg) decreased the locomotor activity, but CPE itself did not induce sleep. However, CPE reduced sleeping onset and prolonged sleeping time induced by pentobarbital (42 mg/kg). In addition, CPE (2 ${\mu}g/ml$) and pentobarbital (2.5 ${\mu}M$) itself did not affect on the chloride influx in primary cultured cerebellar granule cells, but the combination of CPE and pentobarbital (2.5 ${\mu}M$) increased the chloride influx onto the cells. In conclusion, it is suggested that CPE might augment pentobarbital-induced sleep through the increase of chloride influx.
Keywords
Longanae Arilus; Chrysanthemi Flos; Zizyphi Fructus; Ginseng Radix; Sleeping onset time; Sleeping time; Chloride ion;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
연도 인용수 순위
1 De Sousa, F.C., Pereira, B.A., Lima, V.T., Lacerda, C.D., Melo, C.T., Barbosa-Filho, J.M., Vasconcelos, S.M., Viana, G.S. Central nervous system activity of yangambin from Ocotea duckei Vattimo (Lauraceae) in mice. Phytother. Res. 19(4):282-286, 2005.   DOI   ScienceOn
2 Chu, Q.P., Wang, L.E., Cui, X.Y., Fu, H.Z., Lin, Z.B., Lin, S.Q., Zhang, Y.H. Extract of Ganoderma lucidum potentiates pentobarbital-induced sleep via a GABAergic mechanism. Pharmacol. Biochem. Behav. 86(4):693-698, 2007.   DOI   ScienceOn
3 Martinez, A.L., Dominguez, F., Orozco, S., Chavez, M., Salgado, H., Gonzalez, M., Gonzalez-Trujano, M.E. Neuropharmacological effects of an ethanol extract of the Magnolia dealbata Zucc. leaves in mice. J, Ethnopharmacol. 106(2):250-255, 2006.   DOI   ScienceOn
4 Lolli, L.F., Sato, C.M., Romanini, C.V., Villas-Boas, Lde. B., Santos, C.A., de Oliveira, R.M. Possible involvement of GABA A-benzodiazepine receptor in the anxiolytic-like effect induced by Passiflora actinia extracts in mice. J, Ethnopharmacol. 111(2):308-314, 2007.   DOI   ScienceOn
5 Silva, M.I., de Aquino, Neto M,R., Teixeira, Neto P.F., Moura, B.A., do Amaral, J.F., de Sousa, D.P., Vasconcelos, S.M., de Sousa, F.C. Central nervous system activity of acute administration of isopulegol in mice. Pharmacol. Biochem. Behav. 88(2):141-147, 2007.   DOI   ScienceOn
6 Ma, Y., Eun, J.S., Lee, K.S., Lee, E.S., Kim, C.S., Hwang, B.Y., Oh, K.W. Methanol extract of Longanae Arillus regulates sleep architecture and EEG power spectra in restraint-stressed rats. Natural Product Sciences 15(4):213-221, 2009.
7 Yang, S.L., Nam, S.Y., Han, J.Y., Kim, J.C., Lee, K.N., Hong, J.T., Oh, K.W., Eun, J.S. Alterations of spontaneous sleep architecture and cortical electroencephalogram power spectra by Red Ginseng extract via GABAAergic systems. J. Ginseng Res. 34(4):304-313, 2010.   DOI
8 Okuyama, E., Ebihara, H., Takeuchi, H., Yamazaki, M. Adenosine, the anxiolytic-like principle of the Arillus of Euphoria longana. Planta Medica 65: 115-119, 1999.   DOI   ScienceOn
9 Ma, Y., Ma, H., Eun, J.S., Nam, S.Y., Kim, Y.B., Hong, J.T., Lee, M.K., Oh, K.W. Methanol extract of Longanae Arillus augments pentobarbital-induced sleep behaviors through the modification of GABAergic systems. J. Ethnopharmacology. 122: 245-250, 2009.   DOI   ScienceOn
10 Kim, J.W., Han, J.Y., Hong, J.T., Li, R., Eun, J.S. and Oh, K.W. Ethanol Extract of the Flower Chrysanthemum morifolium Augments Pentobarbital-Induced Sleep Behaviors: Involvement of Cl- Channel Activation. Evid. Based Complement. Alternat. Med. p 109164, 2011.
11 Lee, C.I., Kim, J.C., Han, J.Y., Oh, E.H., Oh, K.W., Eun, J.S. Repeated administration of Korea Red Ginseng extract increases non-rapid eye movement sleep via GABAAergic systems. J. Ginseng Res. 36(4):403-410, 2012.   DOI   ScienceOn
12 Hu, Z., Kim, C.S., Oh, E.H., Lee, M.K., Eun, J.S., Hong, J.T., Oh, K.W. Methanol extract of Zizyphi Spinosi Semen augments pentobarbital-induced sleep through the modification of GABAergic systems. Natural Product Sciences 18(2):67-75, 2012.
13 Lee, Y.H. Chronic intake of Panax Ginseng Extract Stabilizes Sleep and awakeness in food-detrived rats. Kor. J. Ginseng Sci. 15(1):79-81, 1991.
14 Oh, S.H., Oh, K.W., Cho, H.K., Eun, J.S. Effects of the combined-preparation of Germinated Brown rice, cultured moutain Ginseng and Longanae Arillus on pentobarbitalinduced sleeping time. Kor. J. Oriental Physiology & Pathology 24(4):598-601, 2010.
15 Pang, J.Y., Lee, M.K., Seo, S.Y., Jeon, H., Kim, D.K., Oh, K.W., Cho, H.K., Eun, J.S. Study on sleeping behaviors of the combined-preparation of crude drugs. Kor. J. Oriental Physiology & Pathology 25(6):996-999, 2011.
16 Eun, J.S., Bae, K. Yun, Y.P., Hong, J.T., Kwon, H.N., Oh. K.W. Inhibitory effects of paenol on morphine-induced locomotor sensitization and conditioned place preference in mice. Arch. Pharm. Res. 29: 904-910, 2006.   DOI
17 Anca, J.M., Lamela, M., Gato, M.A., Cadavid, I., Calleja, J.M. Activity on the central nervous system of Himanthalia elongata; Part II. Planta Med. 59(2):101-105, 1993.   DOI   ScienceOn
18 West, M.R., Molloy, C.R. A microplate assay measureing chloride ion channel activity. Analytical Biochemistry. 241(1):51-58, 1996.   DOI   ScienceOn
19 Kazung, B.G., Masters, S.B., Trevor, A.J. Basic and clinical pharmacology. 11th, McGrawHill, pp 371-383, 2009.
20 全國韓醫科大學 本草學敎授 共編著. 本草學. 圖書出版 永林社. pp 585-586, 1995.