• Animal cells and systems
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• v.1 no.1
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• pp.93-98
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• 1997

The present study investigated the cellular localization of a-subunit of Gq (Gaq) protein in developing L8E63, rat skeletal muscle cell line. The colocalization of Gaq with actin cytoskeleton was demonstrated by double-labeling experiments. In mononucleated myoblasts, the immuno-fluorescence staining pattern of Gaq was almost identical with that of F-actin visualized with rhodamine-conjugated phalloidin. However, this colocalization of Gaq with cytoskeleton was not maintained in multinucleated myotubes. The staining pattern of Gaq in myotubes did not match with any specific subcellular structure, but appeared as a uniformly distributed diffuse staining throughout the whole cell surface. Interestingly, change in the expression level of Gaq was not detected during myoblast differentiation, suggesting that actin-associated Gaq protein might dissociate from the cytoskeleton as cells differentiate. Immunocytochemical experiments using specific antibodies directed against several G proteins indicated that the subcellular localizations of Gai1, Gai2, Gai3, and Gao were different from those obtained with Gaq.

• Bulletin of the Korean Mathematical Society
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• v.59 no.4
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• pp.917-928
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• 2022

Let R be a commutative Noetherian ring and I an ideal of R. In this paper, we study colocalization of generalized local homology modules. We intend to establish a dual case of local-global principle for the finiteness of generalized local cohomology modules. Let M be a finitely generated R-module and N a representable R-module. We introduce the notions of the representation dimension r^I(M, N) and artinianness dimension a^I(M, N) of M, N with respect to I by r^I(M, N) = inf{i ∈ ℕ₀ : H^I_i(M, N) is not representable} and a^I(M, N) = inf{i ∈ ℕ₀ : H^I_i(M, N) is not artinian} and we show that a^I(M, N) = r^I(M, N) = inf{r^IR_𝔭 (M_𝔭,𝔭N) : 𝔭 ∈ Spec(R)} ≥ inf{a^IR_𝔭 (M_𝔭,𝔭N) : 𝔭 ∈ Spec(R)}. Also, in the case where R is semi-local and N a semi discrete linearly compact R-module such that N/∩_t>0I^tN is artinian we prove that inf{i : H^I_i(M, N) is not minimax}=inf{r^IR_𝔭 (M_𝔭,𝔭N) : 𝔭 ∈ Spec(R)＼Max(R)}.

• Bulletin of the Korean Mathematical Society
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• v.57 no.1
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• pp.167-177
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• 2020

Let I be an ideal of Noetherian local ring (R, m) and M an artinian R-module. In this paper, we study colocalization of local homology modules. In fact we give Colocal-global Principle for the artinianness and minimaxness of local homology modules, which is a dual case of Local-global Principle for the finiteness of local cohomology modules. We define the representation dimension r^I (M) of M and the artinianness dimension a^I (M) of M relative to I by r^I (M) = inf{i ∈ ℕ₀ : H^I_i (M) is not representable}, and a^I (M) = inf{i ∈ ℕ₀ : H^I_i (M) is not artinian} and we will prove that i) a^I (M) = r^I (M) = inf{r^IR_𝖕 (_𝖕M) : 𝖕 ∈ Spec(R)} ≥ inf{a^IR_𝖕 (_𝖕M) : 𝖕 ∈ Spec(R)}, ii) inf{i ∈ ℕ₀ : H^I_i (M) is not minimax} = inf{r^IR_𝖕 (_𝖕M) : 𝖕 ∈ Spec(R) ∖ {𝔪}}. Also, we define the upper representation dimension R^I (M) of M relative to I by R^I (M) = sup{i ∈ ℕ₀ : H^I_i (M) is not representable}, and we will show that i) sup{i ∈ ℕ₀ : H^I_i (M) ≠ 0} = sup{i ∈ ℕ₀ : H^I_i (M) is not artinian} = sup{R^IR_𝖕 (_𝖕M) : 𝖕 ∈ Spec(R)}, ii) sup{i ∈ ℕ₀ : H^I_i (M) is not finitely generated} = sup{i ∈ ℕ₀ : H^I_i (M) is not minimax} = sup{R^IR_𝖕 (_𝖕M) : 𝖕 ∈ Spec(R) ∖ {𝔪}}.

• Journal of Pharmacopuncture
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• v.3 no.1
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• pp.65-87
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• 2000

This study was designed to evaluate the effect of the bee venom(BV) aqua-acupuncture on the neuronal activities of catecholaminergic(tyrosine hydroxylase : TH, dopamine ${\beta}$ hydroxylase : D${\beta}$H) system in the brainstem. After the BV aqua-acupuncture was applied on Chok-Samni(ST36) and the gluteal part(Blank locus) in rats. Also, the number of colocalization between catecholamine containing neurons and Fos immunoreactive neurons were analyzed by using the double immunohistochemical technique. The results of the experiments were summarized as follows : 1. In DR and LC, Chok-Samni group and the Blank locus group showed more significant increase in the number of colocalization between TH containing neurons and Fos immunoreactive neurons than the control group. Furthermore, Chok-Samni group showed more significant increase than the Blank locus group. Also, in Arc, Chok-Samni group showed more significant increase than the Blank locus group and the control group. 2. In LC, Chok-Samni group showed more significant increase in the number of colocalization between D${\beta}$H containing neurons and Fos immunoreactive neurons than the Blank locus group and the control group. Also, in A5, Chok-Samni group and the Blank locus group showed more significant increase than the control group. Chok-Samni group showed more significant increase than the Blank locus group. However, there was no significant change in A7. Consequently, the BV aqua-acupuncture increased more potent the number of Fos immunoreactive neurons and the activity of catecholaminergic neurons. Furthermore, the BV aqua-acupuncture was more effective on Chok-Samni than Blank locus group. These results indicate that the BV aqua-acupuncture is very effective therapy to control pain. The therapeutic effect of BV aqua-acupunture may associated with the endogenous modulatory system such as catecholamine. Those data from the study can be applied to establish the effective treatment of the BV for pain control in the clinical field.

• Proceedings of the Korean Society of Developmental Biology Conference
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• 2002.02a
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• pp.24-28
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• 2002

• Korean Journal of Veterinary Research
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• v.37 no.4
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• pp.719-726
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• 1997

Dorsal root ganglion(DRG) cells are primary sensory neurons which contain some biologically active neuropeptides which play a role as neurotransmitters or neuromodulators. This study was performed to observe normal distribution of calcitonin gene-related peptide (CGRP) and substance P (SP) immunoreactive cells and colocalization of CGRP and SP in a single DRG cell of the lumbar DRGs($L_1{\sim}L_6$) in the Wistar Kyoto(WKY) rat by immunohistochemistry. About 55.8% of DRG cells contained CGRP-immunoreactivity, while about 12.7% of DRG cells showed SP-immunoreactivity. There was no significant difference in percentage of each neuropeptied-immunoreactive cells between each neuropeptide-immunoreactive cells between each levels of DRGs ($L_1{\sim}L_6$) (p>0.01). In size distribution, CGRP-immunoreactive cells were identified below $1,500{\mu}m^2$; SP-immunoreactive cells below $600{\mu}m^2$. In serial sections, about 86.7% of the SP immunoreactive cells contained CGRP immunoreactivity.

• Journal of Life Science
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• v.23 no.7
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• pp.941-946
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• 2013

Local protein synthesis at subsynaptic sites plays a key role in the regulation of the protein composition in local domains. In this study, we carried out immunocytochemistry of cultured rat hippocampal neurons in various developmental stages to investigate the expression of eIF4E and its binding protein, eIF4EBP1. Both proteins were distributed in dendrites. In addition, eIF4EBP1 was highly expressed in the nucleus throughout the development, whereas eIF4E was not expressed in the nucleus. Punctate expression of eIF4E and eIF4EBP1 was evident in DIV 3. The colocalization rates of eIF4E or eIF4EBP1 puncta with PSD95 were higher in the dendrogenic than in the mature stages. In contrast, the colocalization rates of eIF4E and eIF4EBP1 puncta were higher in the mature than in the dendrogenic stages. As eIF4E is inactive when it is bound to eIF4EBP1, these data indicate that most dendritic eIF4E's are active during development but that they are mostly under inhibition in mature neurons.

• Applied Microscopy
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• v.25 no.1
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• pp.122-129
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• 1995

Pancreatic endocrine cells of the native Korean goat were investigated immunocytochemically at electron microscopic level. All glucagon-, insulin-, somatostatin- and pancreatic polypeptide(PP)-immunoreactive cells were showed chromogranin(CG) immunoreactivity in the secretory granules of each cells. In addition, bovine pancreatic polypeptide immunoreactivity was found to be colocalized in the secretory granules of the glucagon and insulin cells. These observations support that chromogranin is available as the marker of pancreatic endocrine cells on the native Korean goat and BPP colocalized in the secretory granules of glucagon and insulin cells.

• Journal of Microbiology and Biotechnology
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• v.27 no.3
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• pp.616-623
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• 2017

Ginsenoside Rg3, a saponin extracted from ginseng, has various pharmacological and biological activities; however, its effects against Brucella infection are still unclear. Herein, the inhibitory effects of ginsenoside Rg3 against intracellular parasitic Brucella infection were evaluated through bacterial infection, adherence assays, and LAMP-1 colocalization, as well as immunoblotting and FACS for detecting MAPK signaling proteins and F-actin polymerization, respectively. The internalization, intracellular growth, and adherence of Brucella abortus in Rg3-treated RAW 264.7 cells were significantly decreased compared with the Rg3-untreated control. Furthermore, an apparent reduction of F-actin content and intensity of F-actin fluorescence in Rg3-treated cells was observed compared with B. abortus-infected cells without treatment by flow cytometry analysis and confocal microscopy, respectively. In addition, treating cells with Rg3 decreased the phosphorylation of MAPK signaling proteins such as ERK 1/2 and p38 compared with untreated cells. Moreover, the colocalization of B. abortus-containing phagosomes with LAMP-1 was markedly increased in Rg3-treated cells. These findings suggest that ginsenoside Rg3 inhibits B. abortus infection in mammalian cells and can be used as an alternative approach in the treatment of brucellosis.

• Korean Journal of Veterinary Research
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• v.51 no.1
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• pp.15-20
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• 2011

In this study, we isolated 12 of Brucella (B.) spp. from cattle, which have been positive in Rose Bangal test and tube agglutination test in Gyeongbuk province in 2009. According to AMOS PCR analysis, isolated 12 strains were identified as B. abortus. Murine derived macrophage, RAW 264.7 cells, were infected with isolated 12 strains or reference strain (B. abortus 544), and bacterial internalization were characterized. According to these results, we divided the isolated strains into the following three groups: class I, lower internalization than that of B. abortus 544; class II, similar internalization to that of that of B. abortus 544; class III, higher internalization than that of B. abortus 544 within RAW 264.7 cells. Furthermore, intracellular growth, bacterial adherent assay, LAMP-1 colocalization, virulence in mice and surface protein pattern were characterized. From these results, representative strains of class III showed lower LAMP-1 colocalization, higher adherent efficiency, higher virulence in mice than those of B. abortus 544, and showed different pattern of surface proteins. These results suggest that B. abortus field strains, isolated from cattle in Korea, possess various virulence properties and higher internalization ability of field strain may have an important role for its virulence expression.