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Effect of Carrier on Labeling and Biodistribution of Re-188-Hydroxyethylidene diphosphonate (담체가 Re-188-Hydroxyethylidene Diphosphonate의 표지와 생체내분포에 미치는 영향)

  • Chang, Young-Soo;Jeong, Jae-Min;Kim, Bo-Kwang;Cho, Jung-Hyuk;Lee, Dong-Soo;Chung, June-Key;Lee, Seung-Jin;Jin, Ren-Jie;Lee, Sang-Eun;Lee, Myung-Chul
    • The Korean Journal of Nuclear Medicine
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    • v.34 no.4
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    • pp.344-352
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    • 2000
  • Purpose: Re-188-Hydroxyethylidene diphosphonate (HEDP) is a new cost-effective agent for systemic radioisotope therapy of metastatic bone pain. We investigated the influence of carrier for labeling and biodistribution of Re-188-HEDP using HEDP kit with or without carrier ($KReO_4$). Materials and Methods: The kits (HEDP 15 mg, gentisic acid 4 mg and $SnCl_2.2H_2O_2$ 4.5 mg) with or without carrier ($KReO_4$ 0.1 mg) were labeled with Re-188 solution, made available from an in-house generator by boiling for 15 min. We compared the labeling efficiency and stability of carrier-added and carrier-free preparations of Re-188-HEDP Biodistribution and imaging studies of each preparation were performed in ICR mice ($1.85{\sim}3.7MBq/0.1ml$) and SD rats ($74.1{\sim}85.2MBq/0.5ml$). Results: The carrier-added preparation showed high labeling efficiency (95% at pH 5) and high stability in serum (88%, 3 hr). However, the carrier-free preparation showed low labeling efficiency (59% at pH 5) and low stability (43%, 3 hr). The carrier-added preparation showed high uptake in bone and low uptake in stomach and kidneys. However, the carrier-free preparation showed lower uptake in bone and higher uptake in both stomach and kidneys, which is supposed to be due to released perrhenate. The carrier-added preparation also showed better images with higher skeletal accumulation, lower uptake in other organs and lower soft tissue uptake than the carrier-free preparation. Conclusion: The results of these studies clearly demonstrate that addition of carrier perrhenate is required for high labeling efficiency, stability, bone uptake and good image quality of Re-188-HEDP.

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Whole Mount Preparation of Primary Cultured Neuron for HVEM Observation (배양된 시경세포 관찰을 위한 초고압전자현미경 홀마운트 시료제작기법)

  • Kim, Hyun-Wook;Hong, Soon-Taek;Oh, Seung-Hak;Park, Chang-Hyun;Kim, Hyun;Rhyu, Im-Joo
    • Applied Microscopy
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    • v.41 no.1
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    • pp.69-73
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    • 2011
  • High-voltage electron microscope (HVEM) has higher resolution and penetration power than conventional transmission electron microscope that could be load thick specimen. Some researchers have taken this advantage of HVEM to explore 3-dimensional configuration of the biological structures including tissue and cells. Whole mount preparations has been employed to study some cell lines and primary culture cells. In this study, we would like to introduce useful whole mount preparation method for neuronal studies. The plastic coverslips were punched, covered by formvar membrane and coated with carbon. The neurons obtained embryonic 18 rat hippocampus were seeded on the prepared cover slip. The coverslips were fixed, dried in freeze drier and kept in a descicator until HVEM observation. We could observe detailed neuronal structures such as soma, dendrite and spine under HVEM without conventional thin section and heavy metal stain. The anaglyphic image based on stereo paired image ($-8^{\circ},+8^{\circ}$) provides three dimensional perception of the neuronal dendrites and their spines. This method could be applied to sophisticated analysis of dendritic spine under the various experimental conditions.

Self-efficacy and Preparation of Smoking Cessation in Service and Sales Woman Smokers Working in Department Stores (백화점 서비스 및 판매업 종사 흡연 여성 근로자의 금연자기효능감과 금연준비도)

  • Ha, Yeongmi;Park, Ki-Soo;Choi, Hyeok;Yang, Seungkyoung
    • Korean Journal of Occupational Health Nursing
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    • v.25 no.3
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    • pp.168-176
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    • 2016
  • Purpose: The purpose of this cross-sectional study was to describe smoking characteristics, and then to examine relationships between self-efficacy for smoking cessation and preparation of smoking cessation in service and sales woman employee with smoking in department stores. Methods: This study was a secondary data analysis carried by the smoking cessation support center. One hundred six employees smoking women participated from two department stores. Collected data were analyzed using SPSS 20.0 program. Descriptive statistics, t-test, one-way ANOVA along with $Scheff{\acute{e}}$ test, pearson correlation coefficients were used for statistics. Results: The study results have shown that the mean score of self-efficacy for smoking cessation $5.1{\pm}2.62$, preparation smoking cessation $4.0{\pm}2.89$. There was positive relation between self-efficacy for smoking cessation and preparation smoking cessation. Conclusion: Considering the findings from this study, there is a need to develop self-efficacy for smoking cessation program for effective smoking cessation in service and sales woman smokers employee.

SEM STUDY ON THE BACTERIAL ADHESION TO THE DENTIN OF THE ROOT CANAL (미생물의 근관내 상아질 부착에 대한 주사전자현미경적 연구)

  • Jeong, Sang-Kyun;Oh, Tae-Seok;Bae, Kwang-Shik
    • Restorative Dentistry and Endodontics
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    • v.25 no.1
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    • pp.116-122
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    • 2000
  • On the instrumented root canal wall, amorphous, irregular smear layer can be observed with Scanning Electron Microscope(SEM). The purpose of this study was to evaluate the effect of the presence or absence of smear layer on the adhesion of Staphylococcus aureus to the dentin of the root canal. Human incisors, extracted within 7 days, with no caries, no fracture, no calcification of canal, were selected. After cutting crown portion at cemento-enamel junction, root canal preparation was done by modified crown-down technique using Profile and Gates - Glidden Drill. During canal preparation, 10ml physiologic saline solution(group1&3) or 10ml 3.5% NaOCl(group2&4) was used as irrigation solution. And 10ml physiologic saline solution(group1&3) or 10ml 0.5M EDTA(group2&4) was applicated for final flush. After vertical sectioning and ethylene oxide gas sterilization, samples(group1&2) were immersed into BHIYHM broth inoculated with Staphylococcus aureus (ATCC 31153) and incubated for 3hrs at $37^{\circ}C$. All samples were prepared for and observed with SEM(JEOL JSM840S). The data were analyzed by Mann-Whitney rank sum test. The conclusions are as follows ; 1. Smear layer covers entire root canal surface after root canal preparation. 2. Smear layer has been removed away and the entrances of dentinal tubules have opened widely, when applying 0.5M EDTA and 3.5% NaOCl. 3. A significantly higher number of bacteria were adhered to the root canal dentin without smear layer(p<0.0001). 4. Smear layer produced during root canal preparation impedes bacterial adhesion and colonization to dentin matrix, therefore inhibits canal reinfection.

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Fracture load and survival of anatomically representative monolithic lithium disilicate crowns with reduced tooth preparation and ceramic thickness

  • Nawafleh, Noor A;Hatamleh, Muhanad M;Ochsner, Andreas;Mack, Florian
    • The Journal of Advanced Prosthodontics
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    • v.9 no.6
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    • pp.416-422
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    • 2017
  • PURPOSE. To investigate the effect of reducing tooth preparation and ceramic thickness on fracture resistance of lithium disilicate crowns. MATERIALS AND METHODS. Specimen preparation included a standard complete crown preparation of a typodont mandibular left first molar with an occlusal reduction of 2 mm, proximal/axial wall reduction of 1.5 mm, and 1.0 mm deep chamfer (Group A). Another typodont mandibular first molar was prepared with less tooth reduction: 1 mm occlusal and proximal/axial wall reduction and 0.8 mm chamfer (Group B). Twenty crowns were milled from each preparation corresponding to control group (n=5) and conditioned group of simultaneous thermal and mechanical loading in aqueous environment (n=15). All crowns were then loaded until fracture to determine the fracture load. RESULTS. The mean (SD) fracture load values (in Newton) for Group A were 2340 (83) and 2149 (649), and for Group B, 1752 (134) and 1054 (249) without and with fatigue, respectively. Reducing tooth preparation thickness significantly decreased fracture load of the crowns at baseline and after fatigue application. After fatigue, the mean fracture load statistically significantly decreased (P<.001) in Group B; however, it was not affected (P>.05) in Group A. CONCLUSION. Reducing the amount of tooth preparation by 0.5 mm on the occlusal and proximal/axial wall with a 0.8 mm chamfer significantly reduced fracture load of the restoration. Tooth reduction required for lithium disilicate crowns is a crucial factor for a long-term successful application of this all-ceramic system.

The Effects of Self-Leadership on Career Decision-making Self-efficacy and Career Preparation Behavior - Focused on Culinary Major Students - (셀프리더십이 진로결정 자기효능감 및 진로준비행동에 미치는 영향 - 조리전공 대학생을 중심으로 -)

  • Seo, Kyung-Hwa
    • Culinary science and hospitality research
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    • v.23 no.2
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    • pp.146-158
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    • 2017
  • This study conducted a survey to investigate career decision-making self-efficacy, and career preparation behavior related to self-leadership among students major in culinary area. Frequency analysis, factor analysis, reliability analysis and multiple regression analysis were employed using SPSS 16.0 Version statistical package program, and various results were discovered. First, all the subfactors of self-leadership factors (behavior focused, natural reward, and constructive thought strategy) had a positive effect on career decision-making self-efficacy factors (occupational information, goal setting, career planning, problem solving, self-appraisal). Second, all the factors of self-leadership factors (behavior focused, natural reward and constructive thought strategy) had a positive effect on career preparation (information search activity, goal achievement activity). As a result, the study confirmed that strengthening of self-leadership for culinary major college/university students is important predictor for development with career decision-making self-efficacy and career preparation behavior.

Marginal accuracy and fracture strength of Targis/Vectris Crowns prepared with different preparation designs

  • Song, Ho-Yong;Cho, Lee-Ra
    • The Journal of Korean Academy of Prosthodontics
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    • v.38 no.6
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    • pp.791-799
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    • 2000
  • Statement of problem. Targis/Vectris restorations provide excellent esthetics. Marginal accuracy is significantly influenced by the preparation design. There were no studies to examine the effect of preparation design on the marginal discrepancy and fracture strength of Targis / Vectris crowns. Purpose. This study evaluated the marginal accuracy before and after cementation, and the fracture strength of FRC/Ceromer(Targis / Vectris) crowns according to different preparation design. Material and method. Three metal dies with different convergence angles($6^{\circ},\;10^{\circ},\;15^{\circ}$) were prepared. Total 30 (10 for each angle) Targis/Vectris crowns were made. The restorations were evaluated for adaptation of the margin before and after cementation, then were compressively loaded to failure. Fracture surfaces of the crowns were examined using a SEM. Results. The mean marginal gap was $49{\yen}m\;for6^{\circ},\;55{\S}>for\;10^{\circ}\;and\;70{\S}>for\;15^{\circ}$ and in clinically acceptable level. The mean marginal gap increased significantly after cementation. The increasing amount during cementation was the largest in the $6^{\circ}$ group. The crowns on 60 convergence angle had a significantly higher fracture strength than the crowns on $15^{\circ}$ angle. Mean fracture strength of total crowns regardless of convergence angle was 1390 N, which was higher than all-ceramic crowns. SEM observation showed two-mode fracture pattern. Conclusion. From the results of this study, all of the FRC/Ceromer crowns had clinically acceptable marginal accuracy and could withstand the bite force. Moreover, less convergent angle than all-ceramic crown might be recommended for preparation procedure.

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A COMPARATIVE STUDY ON THE APICAL LEAKAGE OF RETROFILLING AFTER APICAL CAVITY PREPARATION WITH ULTRASONIC INSTRUMENT (초음파 기구에 의한 치근단 와동형성후 역충전의 밀폐성에 대한 연구)

  • Lee, Hyeong-Il;Son, Ho-Hyun
    • Restorative Dentistry and Endodontics
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    • v.18 no.1
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    • pp.197-204
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    • 1993
  • This study was conducted to evaluate and compare the apical leakage in the following retrofilling techniques after apical resection; No apical cavity preparation and no retrofilling(control group), Amalgam(group I) or silver glass ionomer cement(group II) retrofilling after apical cavity preparation with mini contra-angle and bur, Amalgam(group III) or silver glass ionomer cement(group N) retrofilling after apical cavity preparation with ultrasonic micro endo tip. Extracted ninety upper anterior and lower canine teeth were fixed in skull simulators and root canals were prepared with step-back method and obturated with gutta-percha and zinc oxide eugenol sealer. Obturated roots were resected 2mm from apical ends and apical cavities of 1mm width and 2mm depth were prepared and retrofilled by above mentioned methods. After application of nail varnish on all surface except resected surface, apical 1/3 of the roots were placed in 1% methylene blue solution for 3 days. After longutudinal sectioning to expose central parts of filled materials, depths of penetrated dye were measured by measuring microscope and were analyzed statistically. The results were as follows. 1. Having no relation with instruments used in apical cavity preparation, amalgam retrofilling groups(group I and II) showed less apical leakage which was not significant statistically than no retrofilling group(control group) (P<0.05), but silver glass ionomer cement retrofilling gruoups(group II and IV) showed significantly less apical leakage than no retrofilling group(control group) (P<0.01). 2. In the groups retrofilled with the same material, the apical leakage in cavities prepared with ultrasonic micro endo tip (group III and IV) was less than that in cavities prepared with mini contra-angle and bur(group I and II), but not significant statistically(P>0.05). 3. When apical cavities were prepared with same instrument. the egroups retrofilled with silver glass ionomer cement(group II and IV) showed significantly less apical leakage than the groups retrofilled with amalgam(group I and III)(<0.01).

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