• 생명과학회지
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• 제16권7호
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• pp.1219-1224
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• 2006

여성의 폐경에 의한 estrogen의 감소는 골다공증을 유발시킨다고 알려져 있다. 최근 새로운 식품으로 각광받고 있는 매생이 추출물을 인위적 갱년기 장애를 유발시킨 흰쥐에 투여하여 혈 중 ALP활성 변화와 골 중의 collagen의 함량 및 collagen 가교물질 pyridinoline과 deoxypyridinoline 함량 변화를 측정하여 그 효과를 검토해 보았다. 매생이 추출물을 갱년기를 유도한 흰쥐에 투여하여 혈 중 ALP활성 변화에 미치는 영향을 검토한 결과 난소절제에 의하여 ALP활성이 증가하였으나, 매생이 추출물 투여한 군에서 ALP활성이 억제되는 것을 확인할 수 있었다. 또한 난소절제에 의해 연골의 collagen 함량이 감소되었는데, 이는 매생이 추출물을 투여함으로써 정상적인 수치로 증가하는 경향을 나타내어 매생이 추출물이 골 대사와 관련된 골 대사질환에 유효한 효과를 나타낼 것으로 추측된다. 또한 collagen 가교물질로써 골대사의 biomaker인 pyridinoline 및 deoxypyridinoline의 연골 중의 함량은 난소절제에 의해 감소하였고, 매생이 추출물 투여에 의해 증가하여 estrogen부족으로 인한 골 손실에 매생이 추출물이 유익한 효과를 가지는 것으로 보이며, 이는 매생이 중에 함유되어 있는 phytoestrogen에 의한 것으로 추측되어 앞으로 구체적인 검토가 필요한 것으로 사료된다.

• 한국축산식품학회지
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• 제35권4호
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• pp.431-440
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• 2015

Chicken bone is not adequately utilized despite its high nutritional value and protein content. Although not a common raw material, chicken bone can be used in many different ways besides manufacturing of collagen products. In this study, a multi-step procedure was optimized to isolate chicken bone collagen for higher yield and quality for manufacture of collagen products. The chemical composition of chicken bone was 2.9% nitrogen corresponding to about 15.6% protein, 9.5% fat, 14.7% mineral and 57.5% moisture. The lowest amount of protein loss was aimed along with the separation of the highest amount of visible impurities, non-collagen proteins, minerals and fats. Treatments under optimum conditions removed 57.1% of fats and 87.5% of minerals with respect to their initial concentrations. Meanwhile, 18.6% of protein and 14.9% of hydroxyproline were lost, suggesting that a selective separation of non-collagen components and isolation of collagen were achieved. A significant part of impurities were selectively removed and over 80% of the original collagen was preserved during the treatments.

• Asian-Australasian Journal of Animal Sciences
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• 제19권7호
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• pp.1059-1064
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• 2006

Six muscles were seamed out randomly from Hanwoo carcasses (n = 12) of each quality grade (quality grades 1, 2 and 3). Samples were analysed for their total and soluble collagen contents, IMCT (intramuscular connective tissue) and Warner-Bratzler shear force (WBSF). Simple correlation (n = 21) was determined for WBSF among major muscles. For LT (longissimus thoracis), total collagen content was significantly higher (p<0.05) for quality grade 3 than those for quality grades 1 and 2. For semitendinosus (ST), semimembranosus (SM), psoas major (PM) and serratus ventralis (SV), total collagen content of quality grade 1 was lowest (p<0.05) of all quality grades. IMCT shear force for gluteus medius (GM) decreased (p<0.05) with better quality grade, and those for other muscles, with the exception of GM, were higher (p<0.05) for quality grade 3 than for quality grades 1 and 2. WBSF values showed GM and LT to be decreased (p<0.05) with better quality grade, and PM to be higher (p<0.05) for quality grade 3 than those for quality grades 1 and 2. SM, ST and SV from quality grade 1 had lower (p<0.05) WBSF value than those from quality grades 2 and 3. Total collagen content of ST was highest (p<0.05) of all muscles, whereas that of PM was lowest (p<0.05). Soluble collagen contents of LT and SV from quality grades 1 and 2 were, in general, higher (p<0.05) than other muscles, but that of SM was lowest (p<0.05). ST and SM had higher (p<0.05) WBSF values for three quality grades when compared to other muscles, whereas PM was lowest (p<0.05). LT had the strongest simple correlation with SV (r = 0.78) and GM (r = 0.77), and SM had the strongest correlation with ST (r = 0.73) and LT (r = 0.73). Also, PM had the strongest correlation with SV (r = 0.62).

• 한국식품과학회지
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• 제27권4호
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• pp.571-575
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• 1995

피부조직 콜라겐의 열안정성에 대한 기초자료를 얻고자 DSC를 이용하여 Tm(transition temperature)와 ${\Delta}H(enthalpy)$를 측정하였다. 수화시간과 수화율에 따른 불용성 콜라겐의 DSC 특성은 수화시간에 비하여 수화율이 콜라겐 구조안정성에 미치는 영향이 큰 것으로 나타났으며, 수화율이 증가할수록 Tm은 낮아지고 ${\Delta}H$는 증가하였다. 주령 및 성별에 따른 acetone dried skin의 Tm 및 ΔH는 암컷의 경우보다 수컷의 경우가 다소 높았으며, 주령이 증가할수록 Tm과 ${\Delta}H$가 서서히 증가하고 있어 열안정성은 나이와 관계가 있음을 보여주었다. Acetone dried skin의 경우와는 달리 염가용성 콜라겐의 DSC 특성값은 암컷의 경우가 수컷의 경우보다 다소 높았으며, 암컷의 Tm과 ${\Delta}H$는 주령의 증가에 따라 유의적으로 감소하는 경향을 보였다. 이러한 결과는 피부조직내에서의 콜라겐 구조는 성별 또는 주령에 따라 달리 변화되고 있음을 간접적으로 시사한 것으로 생각되었다.

• 한국축산식품학회지
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• 제39권2호
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• pp.345-353
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• 2019

Various commercial collagen mixtures aimed at improving the quality of meat products are available, but the optimal composition is unclear. This study aimed to compare the functional properties, including physicochemical characteristics and lipid oxidative stability, of loin ham marinated with three commercial collagen mixtures sold as food additives. The addition of collagen mixtures led to significant increases in the moisture content, water holding capacity (WHC), cooking yield, and instrumental tenderness, regardless of the type of collagen mixture. In particular, meat samples containing collagen mixture C showed the highest (p<0.05) WHC and tenderness among all groups. Furthermore, collagen mixture B induced increases (p<0.05) in pH values in both raw and cooked samples. The $a^*$ values of samples with collagen mixtures were lower (p<0.05) than those of samples without collagen mixtures. All collagen mixtures effectively improved oxidative stability during 7 days of storage at $4^{\circ}C$. The samples containing collagen mixture B had the lowest lipid oxidation (p<0.05) among groups. These results indicated that collagen mixture C could be used in injection brine to enhance the quality characteristics of meat products, particularly the WHC and tenderness. Collagen mixture A could be used for meat products with high fat contents based on its ability to improve lipid oxidative stability during long-term storage.

• 한국수산과학회지
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• 제41권6호
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• pp.427-434
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• 2008

Processing of collagen from yellowfin tuna (Thunnus albacares) abdominal skins was optimized by response surface methodology and central composite design. The values of independent variables at optimal conditions were NaOH concentration: 0.5 N, NaOH treatment time: 36.2 hr, pepsin concentration: 1:4.9 ratio (0.245%, w/v), and digestion time: 48.1 hr, respectively. The collagen content estimated under optimal conditions was 33.1%, and the actual experimental collagen content was 32.3%. Physicochemical properties of collagen from yellowfin tuna abdominal skin were investigated by amino acids analysis, SDS-PAGE, FT-IR, viscosity and denaturation temperature. Amino acids content of the collagen was 21.0%. SDS-PAGE pattern of the collagen showed two different $\alpha$-chain (${\alpha}_1$- and ${\alpha}_2$- chain), $\beta$-component and $\gamma$-component. The spectrum of FT-IR of the collagen showed wavenumber at 3,434, 1,650, 1,542 and $1,235\;cm^{-1}$ representing the regions of amide A, I, II and III, respectively. Relative viscosity of the collagen decreased continuously on heating up to $32^{\circ}C$, and the rate of decrease was retarded in the temperature range of $35-50^{\circ}C$. Denaturation temperature (Td) of the collagen solution (0.06%, w/v) was $31^{\circ}C$ and was lower than calf skin collagen ($35^{\circ}C$).

• 생명과학회지
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• 제15권6호
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• pp.1022-1027
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• 2005

폐경을 앞둔 여성에게는 폐경을 전후하여 지속되는 골 손실 가속화 등의 문제점을 가지고 있다. 그러므로 본 연구에서는 인위적 폐경을 유발시킬 수 있는 난소 절제 쥐에서의 골 손실의 유무를 결합조직 중의 collagen함량 변화를 통하여 그 효과를 검토하였다. 그 결과 생화학적 골 대사 지표인 혈 중 ALP활성은 난소 절제로(OVX-control)로 인하여 증가하는 반면, 난소 절제 후 오디 추출물 투여(OVX-EE, OVX-EA)로 혈 중 ALP활성은 감소하는 경향을 보여 골 흡수를 저하시킬 수 있는 가능성을 나타내었다. 한편 난소 절제 후 오디 추출물 투여에 의해 폐 조직과 골 $\cdot$ 연골 조직에서 collagen 함량이 증가하는 경향을 보였고, 그 중 연골 조직에서는 collagen 함량의 저하가 정상적으로 회복되었다. 한편 피부 조직에서도 난소 절제 후 오디 추출물 투여군 모두에서 collagen 합성량이 유의적으로 증가하였다. 오디 추출물이 estrogen감소로 인한 collagen 합성저하를 회복시킨 본 실험 결과로 미루어 보아 오디 중의 phytoestrogen이 estrogen유사효과를 나타냄을 알 수 있었고, 국내산 오디뿐만 아니라 중국산 오디에서도 갱년기 유도에 의한 collagen 함량 저하를 회복하는 결과가 나타났으므로 외인성 estrogen 투여로 인한 부작용을 줄여 줄 수 있을 것으로 기대된다. 이상과 같이 폐경기 여성에게 오디의 섭취는 폐경으로 인한 장애를 감소시키기 위하여 estrogen의 장기간 복용에 의한 부작용을 오디중의 phytoestrogen이 estrogen대체 작용을 함으로써 그 부작용을 감소시켜 줄 것으로 사료되며, 이들의 구조 및 기전에 대해서는 앞으로 연구가 더 필요한 것으로 생각된다.

• 동아시아식생활학회지
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• 제23권6호
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• pp.760-767
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• 2013

This study investigated the quality of noodles containing different amounts of collagen powder. Noodles were prepared at ratios of 1, 3, 5 and 7% collagen power based on flour weight. The water binding capacity of the collagen powder was higher than that of flour. The rate of weight increase and volume increase of the noodles as well as the pH and turbidity of the soups were significantly increased with increasing amounts of collagen powder. The noodles showed decreased L and a values, and increased b values with increasing collagen powder in the flour composite. Hardness, springiness and chewiness of cooked noodles increased significantly with the increase in collagen powder content. cohesiveness and brittleness decreased with increasing amounts of added collagen powder. DPPH radical scavenging activity was improved significantly the addition of the collagen powder. Finally sensory evaluation results indicated that the noodles containing 1, 3% collagen powder had higher quality as compared to the other samples. Overall the results suggest that collagen powder is effective for improving the appearance and texture of noodles.

• Food Science and Biotechnology
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• 제16권4호
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• pp.549-556
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• 2007

The study was carried out to examine on the refiner discharge from Alaska pollock as a collagen resource by characterizing biochemical and functional properties of collagen. The refiner discharge from Alaska pollock surimi manufacturing was a good resource for collagen extraction according to the results of total protein, heavy metal, volatile basic nitrogen, collagen content, amino acid composition, and thermal denaturation temperature (TDT). TDT of acid soluble collagen from refiner discharge showed $20.7^{\circ}C$, which was similar to that of collagen from Alaska pollock muscle and was higher than that of collagen from Alaska pollock skin. TDT of acid-soluble collagen from refiner discharge was, however, lower than those of skin collagens from warm fish and land animal. Acid-soluble collagen from refiner discharge of Alaska pollock could be used as a functional ingredient for food and industrial applications according to the results of water and oil absorption capacities, and emulsion properties. In addition, if the thermal stability of the acid-soluble collagens is improved, collagen from refiner discharge from Alaska pollock could be more effectively used.

• Journal of Periodontal and Implant Science
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• 제5권1호
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• pp.14.2-14.2
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• 1975