Browse > Article
http://dx.doi.org/10.5352/JLS.2005.15.6.1022

Effects of Mulberry Fruits on Collagen Content of Connective Tissues in Ovariectomized Rats  

Choi Kyung-Ha (Department of Food Science and Nutrition, Silla University)
Park Mi-Hwa (Department of Food Science and Nutrition, Silla University)
Kim Mihyang (Department of Food Science and Nutrition, Silla University)
Publication Information
Journal of Life Science / v.15, no.6, 2005 , pp. 1022-1027 More about this Journal
Abstract
The aim of this study was to evaluate effects of mulberry cultivars extracts on the collagen content of the connective tissues in ovariectomized rats. From day 2 until day 37 after ovariectomy, Sprague-Dawley female rats were randomly assigned to the following groups : sham-operated rats (Sham), ovariectomized control rats (OVX-control), and ovariectomized rats supplemented with $80\%$ ethyl alcohol extracts from various kinds of Tajikistan mulberry, Korea mulberry and China mulberry at 200 mg/kg bw/day, respectively. The mulberry extracts were orally administrated at 1 mL per day. The OVX rats were significantly heavier than the sham-operated rats were at all time points, but supplementation with the mulberry extracts tended to gain weight less than OVX-control did. The ovariectomy caused a decreasing in the levels of collagen content in bone and cartilage tissues. However, supplementation with mulberry extracts prevented the decrease in the collagen level in bone and cartilage tissues. Therefore, it may be used to possibly improve the quality of life in menopausal women.
Keywords
collagen; connective tissues; mulberry; ovariectomized rats;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Kim, M. H., M. Otsuka and N. Arakawa. 1994. Age-related changes in the pyridinoline content of guinea pigs cartilage and achilles tendon collagen. J Nutr Sci Vitaminol 40: 95-103   DOI   ScienceOn
2 Kwon, S. C. 1998. Effects of continuously added oral progestin (medroxy progesterone acetate) on the levels of serum lipid and lipoprotein during estrogen replacement therapy in postmenopausal women. Korean Soc. Obstetrics & Gynecology 41: 2442-2446
3 Kuboki, Y. and G. L. Mechanic. 1982. Comparative molecular distribution of cross-links in bone and dentin collagen. Calcif Tissue Int 34: 306-308   DOI   ScienceOn
4 Lee, E. S. and B. H. Kang. 1997. Biochemical bone markers in postmenopausal women. Korean Soc. Obstetrics & Gynecology 40: 1450-1457
5 McConkey, B., G. M. Fraser, A. S. Bligh, H. Whiteley. 1963. Transparent skin and osteoporosis. Lancet 1: 693-695
6 Noda, M. and G. A. Roodon. 1989. Transcriptional regulation of osteopontin pro.duction in rat osteoblast-like cells by parathyroid hormone. J Cell Biol 108: 713
7 Robins, S. P. and A. J. Bailey. 1977. The chemistry of the collagen cross-links. Biochem J 163:339-346
8 Seigel, R. C. 1976. Collagen cross-linking. JBC 251: 5786-5792
9 Stolley, P. D., J. A. Tonascia. M. S. Tockman, P. E. Sartwell, A. H. Rutledge and M. P. Jacobs. 1975. Thrombosis with low-estrogen oral contraceptives. Am J Epidem 102: 197-201
10 The assocition of Korean Clinical Pathology. 1994. The clinical pathology. Korea Medicine Co : 40-79
11 Wickelgren, I. 1997. A new weapon against Alzheimer's. Science 276: 676-677   DOI
12 Wronski, T. J., M. Cintron and L. M. Dann. 1988. Temporal relationship between bone loss and increased bone turnover in ovariectomized rats. Calcif Tissue Int 43: 179-183   DOI   ScienceOn
13 松本俊夫. 1996. 骨粗鬆症. 羊士社. p16
14 Kafantari H, E. Kounadi, M. Fatouros, M. Milonakis and M. Tzaphidou. 2000. Structural alteration in rat skin and bone collagen fibrils induced by ovariectomy. Bone 26: 349-353   DOI   ScienceOn
15 Imble, R. B., J. L. Vannice and D. C. Bloedow. 1994. Interleukin-1 receptor antagonist decreases bone loss and resorption in ovariectomized rats. J Clin Invest 93: 1959-1967   DOI   ScienceOn
16 Jeffcoat, M. K. and C. H. Chesnut. 1993. Systemic osteoporosis and oral bone loss, evidence shows increased risk factors. Jadas 124: 49-56
17 Jeong, C. H, O. S. Joo, K. H. Shim. 2002. Chemical Components and Physiological Activities of Young Mulberry (Morus alba) Stem. Korean journal of Food Preservation 9(2), 228-233
18 Kim, C.W. 1996. The study on treatment of climacteric disorder I(osteoporosis). wonkwang univ 32: 109-136
19 Kim, H. B, H. S. Bang, H. W. Lee, Y. S. Seuk and B. S. Gyoo. 1999. Chemical Characteristics of Mulberry Syncarp. Korean J. Seric. Sci. 41(3), 123-128
20 Kim, I. G., S. B. Kim, J. G. Kim and K. C. Kim. 1993. Serum enzymes as indicators of radiation exposure in rat. Journal of the Korean academy of family medicine 18: 37-44
21 Kim, S. Y, W. C. Lee, H. B. Kim, A. J. Kim and S. K. Kim. 1998. Antihyperlipidemica effects of methanol extracts from Mulberry leaves in cholesterol-induced hyperlipidemia rats. J. Kor. Soc. Food Sci. Nurt 27, 1217-1222
22 Kim, T. W, Y. B. Kwon, Lee, J. H. Yang, I. S. Youm, J. K. Lee, H. S and J. Y. Moon. 1996. A study on the antidiabetic effect of mulberry fruits. Korean. J. Seric. Sci 38(2), 100-107
23 Kim, M. H. 2003. The effects of sedum sarmentosum bung on collagen content of connective tissues in ovariectom ized rats. J Korean Soc Food Sci Nutr 32: 1114-1119   DOI   ScienceOn
24 Mpster, D. W., R. Birchman and R. Xu. 1995. Temporal changes in cancellous bone structure of rats immediately after ovariectomy. Bone 16: 157-161   DOI   ScienceOn
25 Woessner, J. F. 1961. The determination of hydroxyproline in tissue and protein samples containing small proportions of this imino acid. Arch Biochem Biophys 93: 440-447   DOI   ScienceOn
26 Kind, P. R. N. and E. J. King. 1954. Estimation of plasma phosphatase by determination of hydrolyzed phenol with aminoantipyrine. J Clin Pathol 7: 322-326   DOI
27 Clark, A. P. and J. A. Schuttinga. 1992. Targeted estrogen/progesterone replacement therapy for steoporsis. calculation of health care cost savings. Osteoporos Int 2: 195-200   DOI   ScienceOn
28 Colditz, G. A., S. E. Hankinson, D. J. Hunter, W. C. Willett, J. E. Menson, M. J. Stampfer, C. Hennekens, B. Rosner and F. E. Speizer. 1995. The use of estrogens and progenstins and the risk of breast cancer in postmenopausal women. New Engl J Med 332: 1589-1593   DOI   ScienceOn
29 Fujimoto, D., M. Hira and T. Iwa. 1982. Histidinoalanine, a new crosslinking amino acid in calcified tissue collagen. Biochem Biophys Res Comm 104: 1102-1106   DOI   ScienceOn
30 Grodstein, F., M. J. Stamfer, G. A. Colditz, W. C. Willett, J. E. Manson, M. Joffe, B. Rosner, C. Fuchs, S. E. Hankinson, D. J. Hunter, C. H. Hennekens and F. E. Speizer. 1997. Postmenopausal hormone therapy and mortality. New Engl J Med. 336: 1769-1775   DOI   ScienceOn
31 Harris, R. B., A. Laws, F. M. Reddy, A. King and W. L. Haskell. 1990. Are women using postmenopausal estrogens? A community survey. Am J Public Health 80: 1266-1268   DOI   ScienceOn
32 Holland, E.F., J. W. Studd and J. P. Mansell. 1994. Changes in collagen composition and cross-links in bone and skin of osteoporotic postmenopausal women treated with percutaneous estradiol implant. Obstet Gynecol 83: 180-183
33 Baker, H. J., J. R. Lindsey and S. H. Weisbroth. 1984. The laboratory rats. Academic Press Inc, New York, vol II : 123-127
34 Ianghua Liu, E. B. Joanna, Xu Haiyan, Gu Chungang, B. B. Richard, P. L. B. Krishana, B. Nancy, I. C. Andreas, M. P. John, H. S. F. Harry, R. F. Norman and L. B. Juddy. 2001. Evaluation of estrogenic activity of plant extracts for the potential treatment of menopausal syptoms. J Agric Food Chem 49: 2472-2479   DOI   ScienceOn
35 Abe, T, J. W. M. Chow, J. M. Lean and T. J. Chambers. 1993. Estrogen does not restore bone lost after ovariectomy in the rat. J Bone Miner Res 8: 831-838
36 Ahn, H. S, J. R. Kwon and S. S. Lee. 1997. Effect of Dietary Lipids and Guar Gum on Lipid Metabolism in Ovariectomized Rats. Korean Nutr Society 30: 1123-1131
37 Brosage, P. 1995. Hormone therapy: The woman's decision. Contemp Nurse Pract 1(S), 3
38 Bush, T. L. and E. Barret-Connor. 1985. Noncontraceptive estrogen use and cardiovascular disease. Epidemiol Rev 7: 89-104
39 Cha, J. Y., H. J. Jun and Y. S. Cho. 2000. Effect of water-soluble extract from leaves of Morus alba and Cudrania tricuspidata on the lipid concentrations of serum and liver in rats. J. Korean Soc. Agric. Chem. Biotechnol 43, 303-308
40 Campos, H., W. F. Wilson Peter, D. Jimenez, J. R. Mcnamara, J. Ordovas and E. J. Schaefer. 1990. Differences in apolipoproteins and low density lipoprotein subfractions in postmenopausal women on and off estrogen therapy: Results from the Framingham offspring study. Metabolism 39: 1033-1038   DOI   ScienceOn
41 Chen, F., N. Nakashima, I. Kimura and M. Kimura. 1995. Hypoglycemic activity and mechanisms of extracts from mulberry leaves(Folium mori) and cortex moriradicis in streptozotocin-induced diabetic mice. Akugaku Zasshi 15, 476-482