Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Effects of Mulberry Fruits on Collagen Content of Connective Tissues in Ovariectomized Rats

오디가 난소를 절제한 흰쥐 결함조직 중의 collagen 함량 변화에 미치는 영향

  • Choi Kyung-Ha (Department of Food Science and Nutrition, Silla University) ;
  • Park Mi-Hwa (Department of Food Science and Nutrition, Silla University) ;
  • Kim Mihyang (Department of Food Science and Nutrition, Silla University)
  • 최경하 (신라대학교 자연과학대학 식품영양학과) ;
  • 박미화 (신라대학교 자연과학대학 식품영양학과) ;
  • 김미향 (신라대학교 자연과학대학 식품영양학과)
  • Published : 2005.12.01
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Abstract

The aim of this study was to evaluate effects of mulberry cultivars extracts on the collagen content of the connective tissues in ovariectomized rats. From day 2 until day 37 after ovariectomy, Sprague-Dawley female rats were randomly assigned to the following groups : sham-operated rats (Sham), ovariectomized control rats (OVX-control), and ovariectomized rats supplemented with $80\%$ ethyl alcohol extracts from various kinds of Tajikistan mulberry, Korea mulberry and China mulberry at 200 mg/kg bw/day, respectively. The mulberry extracts were orally administrated at 1 mL per day. The OVX rats were significantly heavier than the sham-operated rats were at all time points, but supplementation with the mulberry extracts tended to gain weight less than OVX-control did. The ovariectomy caused a decreasing in the levels of collagen content in bone and cartilage tissues. However, supplementation with mulberry extracts prevented the decrease in the collagen level in bone and cartilage tissues. Therefore, it may be used to possibly improve the quality of life in menopausal women.

폐경을 앞둔 여성에게는 폐경을 전후하여 지속되는 골 손실 가속화 등의 문제점을 가지고 있다. 그러므로 본 연구에서는 인위적 폐경을 유발시킬 수 있는 난소 절제 쥐에서의 골 손실의 유무를 결합조직 중의 collagen함량 변화를 통하여 그 효과를 검토하였다. 그 결과 생화학적 골 대사 지표인 혈 중 ALP활성은 난소 절제로(OVX-control)로 인하여 증가하는 반면, 난소 절제 후 오디 추출물 투여(OVX-EE, OVX-EA)로 혈 중 ALP활성은 감소하는 경향을 보여 골 흡수를 저하시킬 수 있는 가능성을 나타내었다. 한편 난소 절제 후 오디 추출물 투여에 의해 폐 조직과 골 $\cdot$ 연골 조직에서 collagen 함량이 증가하는 경향을 보였고, 그 중 연골 조직에서는 collagen 함량의 저하가 정상적으로 회복되었다. 한편 피부 조직에서도 난소 절제 후 오디 추출물 투여군 모두에서 collagen 합성량이 유의적으로 증가하였다. 오디 추출물이 estrogen감소로 인한 collagen 합성저하를 회복시킨 본 실험 결과로 미루어 보아 오디 중의 phytoestrogen이 estrogen유사효과를 나타냄을 알 수 있었고, 국내산 오디뿐만 아니라 중국산 오디에서도 갱년기 유도에 의한 collagen 함량 저하를 회복하는 결과가 나타났으므로 외인성 estrogen 투여로 인한 부작용을 줄여 줄 수 있을 것으로 기대된다. 이상과 같이 폐경기 여성에게 오디의 섭취는 폐경으로 인한 장애를 감소시키기 위하여 estrogen의 장기간 복용에 의한 부작용을 오디중의 phytoestrogen이 estrogen대체 작용을 함으로써 그 부작용을 감소시켜 줄 것으로 사료되며, 이들의 구조 및 기전에 대해서는 앞으로 연구가 더 필요한 것으로 생각된다.

Keywords

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