• Journal of Microbiology and Biotechnology
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• 제32권12호
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• pp.1599-1604
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• 2022

Storage stability of freeze-dried lactic acid bacteria is a critical factor for their cost-effectiveness. Long-term storage of lactic acid bacteria enables microbial industry to reduce distribution costs. Herein, we investigated the effect of cold adaptation under supercooling conditions at -5℃ on the viability of Leuconostoc mesenteroides WiKim32 during the freeze-drying process and subsequent storage. Cold adaptation increased the thickness of exopolysaccharides (EPS) and improved the viability of freeze-dried Leu. mesenteroides WiKim32. Compared to non-adapted cells, cold-adapted cells showed a 35.4% increase in EPS thickness under supercooling conditions. The viability of EPS-hydrolyzed cells was lower than that of untreated cells, implying that EPS plays a role in protection during the freeze-drying process. Cold adaptation increased the storage stability of freeze-dried Leu. mesenteroides WiKim32. Fifty-six days after storage, the highest viability (71.3%) was achieved with cold adaptation at -5℃. When EPS-containing broth was added prior to the freeze-drying process, the viability further increased to 82.7%. These results imply that cold adaptation by supercooling pretreatment would be a good strategy for the long-term storage of Leu. mesenteroides WiKim32.

• 한국산림과학회지
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• 제78권1호
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• pp.26-29
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• 1989

수목종자(樹木種子)의 포장발아율(圃場發芽率)을 높이는 종자전처리방법(種子前處理方法)을 찾기 위하여, 황벽나무를 비롯한 5수종의 종자(種子)에 대해 종자전처리(種子前處理)에 따른 포장발아율(圃場發芽率)을 조사(調査) 비교(比較)하여 얻은 주요결과(主要結果)는 다음과 같다. 1. 탱자 및 물푸레나무의 포장발아율(圃場發芽率)은 종자전처리간(種子前處理間)에 1% 유의수준(有意水準)에서, 황벽나무는 5% 유의수준(有意水準)에서 각각 통계적(統計的) 유의성(有意性)이 인정(認定)되었으며, 이들 수종(樹種)은 노천이장처리(露天理藏處理)(166 일간(日間)에서 포장발아율(圃場發芽率)이 가장 높았다. 2. 모과나무의 포장발아율(圃場發芽率)은 종자전처리간(種子前處理間)에 1% 유의수준(有意水準)에서 통계적(統計的) 유의성(有意性)이 인정(認定)되었으며, 냉습적처리(冷濕積處理)(110 일간(日間))에서 가장 높았다. 3. 꾸지뽕의 포장발아율(圃場發芽率)은 종자전처리간(種子前處理間)에 5% 유의수준(有意水準)에서 통계적(統計的) 유의성(有意性)이 인정(認定)되지 않았다.

• 한국작물학회지
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• 제33권3호
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• pp.248-253
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• 1988

벼 약배양에 있어서 배양약의 viability의 변화를 관찰하고자 밀양 2003, 대청벼, 치악벼를 재료로 품종 및 저온전처리, 약의 갈변에 따른 호흡율의 변화를 2~3일 간격으로 조사하였다. 화분의 발육시기에 따른 배양전 약의 호흡활성은 개화직전에 가장 높았으며, 배양적기인 stage 3~4에서의 호흡율은 12.46 $O_2$ n mode/ml/h/another이었다. 배양약의 호흡활성의 변화는 3~9일경에 1차 정점을, 9~11일경에 2차 정점을 나타냈으며, Callus 형성능이 높은 대청벼에서 가장 높게 나타났다. 저온전처리는 배양 초기의 호흡활성을 저하시켰으나 15일경부터는 무처리구 보다 높은 경향이었다. 또한 저온전처리는 약의 갈변을 촉진하였으며, 갈변약에서 호흡활성이 높았다.

• The Korean Journal of Pain
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• 제13권2호
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• pp.156-163
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• 2000

Background: Peripheral nerve injury sometimes leads to chronic neuropathic pain such as causalgia. A subset of patients with causalgia have a sympathetically maintained pain which is often evoked by cooling stimuli. However, our knowledge on adrenergic receptor types responsible for cold-evoked pain that is sympathetically dependent is lacking. The present study was conducted to investigate subtypes of adrenoceptors involved in mediating cold-evoked pain that developed following peripheral nerve injury. Methods: Neuropathic surgery was performed by a unilateral ligation of L5 and L6 spinal nerves of rats. Behavioral sign of cold-evoked pain was examined for 5 min by measuring cumulative duration of time that the rat lifted its foot off a metal plate held at cold temperature ($5^{\circ}C$). Whether cold-evoked pain behavior was affected by antagonists of various subtypes of adrenoceptors, which were administered intraperitoneally before and after the ligation, was investigated. Results: After ligation, duration of foot lifting on the ligated side at cold temperature increased as compared to the pre-operative period. This increase maintained for the entire 40-day test period. Pretreatment with alpha-antagonist phentolamine produced a suppression of cold-evoked pain behavior that was not affected by beta-antagonist propranolol pretreatment. Prazosin, alpha-1 antagonist, suppressed cold- evoked pain behavior when treated either before or after nerve ligation. On the other hand, alpha-2 antagonist yohimbine was without effect on cold-evoked pain behavior whether it was treated before or after the ligation. Conclusions: The results suggest that peripheral nerve injury develops cold-evoked pain that is sympathetically dependent, and that alpha-1 adrenoreceptor plays a critical role for the generation of this type of pain in its initiation as well as maintenance.

• Environmental Engineering Research
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• 제24권3호
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• pp.412-417
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• 2019

This paper presents the results of using cold plasma to treat surface water for domestic use purpose. Experimental results showed that cold plasma was an effective method for destroying bacteria in water. After treatment with cold plasma, concentration of coliform and Escherichia coli dramatically reduced. Besides, cold plasma significantly removed water odor, increased dissolved oxygen and decreased the concentration of chemical oxygen demand. However, cold plasma significantly raised the concentration of nitrite and nitrate. Other disadvantages of treating with cold plasma were conductivity increase and pH reduction. Pretreatment steps of coagulation, flocculation, sedimentation and sand filtration followed by disinfection with cold plasma exhibited a high efficiency in surface water treatment. All parameters of surface water after treatment by using the prototype satisfied with the allowance standard of domestic water quality.

• 원예과학기술지
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• 제29권5호
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• pp.488-493
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• 2011

Callus culture initiation of strawberry (Fragaria${\times}$ananassa Duch.) was investigated at different Murashige and Skoog (MS) medium strengths, types and concentrations of plant growth regulators, and incorporating a cold pretreatment period to determine the optimal nutritional and environmental conditions. No high quality callus was induced on MS media without auxin regardless of medium strength. When 6-benzylaminopurine (BA) was combined with indole acetic acid (IAA), naphthalene acetic acid (NAA), and 2,4-dichlorophenoxyacetic acid (2,4-D), high quality callus were highly induced compared to medium supplemented with auxin alone. When $0.5mg{\cdot}L^{-1}$ BA was combined with IAA, NAA, and 2,4-D, high quality callus induction was more effective than the medium supplemented with the other BA concentrations. The best combination of auxin and cytokinin for high quality callus induction was $1.0mg{\cdot}L^{-1}$ NAA and $0.5mg{\cdot}L^{-1}$ BA. Although the differences in callus induction were not significant, high quality callus induction at half strength MS medium was more effective than at full strength medium. When $30g{\cdot}L^{-1}$ sucrose was added to the half strength MS medium, the rate of high quality callus induction increased. The optimum cold pretreatment temperature and period for high quality callus induction were $4^{\circ}C$ and 72 h, respectively. Regeneration rate of high quality callus increased in MS medium supplemented with thidiazuron.

• The Korean Journal of Physiology and Pharmacology
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• 제20권5호
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• pp.467-476
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• 2016

In the present study, we examined the effect of pertussis toxin (PTX) administered centrally in a variety of stress-induced blood glucose level. Mice were exposed to stress after the pretreatment of PTX (0.05 or 0.1 mg) i.c.v. or i.t. once for 6 days. Blood glucose level was measured at 0, 30, 60 and 120 min after stress stimulation. The blood glucose level was increased in all stress groups. The blood glucose level reached at maximum level after 30 min of stress stimulation and returned to a normal level after 2 h of stress stimulation in restraint stress, physical, and emotional stress groups. The blood glucose level induced by cold-water swimming stress was gradually increased up to 1 h and returned to the normal level. The intracerebroventricular (i.c.v.) or intrathecal (i.t.) pretreatment with PTX, a $G_i$ inhibitor, alone produced a hypoglycemia and almost abolished the elevation of the blood level induced by stress stimulation. The central pretreatment with PTX caused a reduction of plasma insulin level, whereas plasma corticosterone level was further up-regulated in all stress models. Our results suggest that the hyperglycemia produced by physical stress, emotional stress, restraint stress, and the cold-water swimming stress appear to be mediated by activation of centrally located PTX-sensitive G proteins. The reduction of blood glucose level by PTX appears to due to the reduction of plasma insulin level. The reduction of blood glucose level by PTX was accompanied by the reduction of plasma insulin level. Plasma corticosterone level up-regulation by PTX in stress models may be due to a blood glucose homeostatic mechanism.

• The Korean Journal of Pain
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• 제27권4호
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• pp.326-333
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• 2014

Background: Nefopam is a centrally acting non-opioid analgesic agent. Its analgesic properties may be related to the inhibitions of monoamine reuptake and the N-methyl-D-aspartate (NMDA) receptor. The antinociceptive effect of nefopam has been shown in animal models of acute and chronic pain and in humans. However, the effect of nefopam on diabetic neuropathic pain is unclear. Therefore, we investigated the preventive effect of nefopam on diabetic neuropathic pain induced by streptozotocin (STZ) in rats. Methods: Pretreatment with nefopam (30 mg/kg) was performed intraperitoneally 30 min prior to an intraperitoneal injection of STZ (60 mg/kg). Mechanical and cold allodynia were tested before, and 1 to 4 weeks after drug administration. Thermal hyperalgesia was also investigated. In addition, the transient receptor potential ankyrin 1 (TRPA1) and TRP melastatin 8 (TRPM8) expression levels in the dorsal root ganglion (DRG) were evaluated. Results: Pretreatment with nefopam significantly inhibited STZ-induced mechanical and cold allodynia, but not thermal hyperalgesia. The STZ injection increased TRPM8, but not TRPA1, expression levels in DRG neurons. Pretreatment with nefopam decreased STZ-induced TRPM8 expression levels in the DRG. Conclusions: These results demonstrate that a nefopam pretreatment has strong antiallodynic effects on STZ-induced diabetic rats, which may be associated with TRPM8 located in the DRG.

• 한국식품과학회지
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• 제45권4호
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• pp.466-472
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• 2013

전처리 방법에 따른 냉풍감압건조 복숭아의 이화학적 및 기능적 특성을 조사한 결과 수분함량은 0.3% NaCl 처리에서 유의적인 낮은 수분함량을 나타내었다. pH의 경우 전처리 구에서 낮아지는 경향을 보였고, 당도 및 당산비는 0.1% Vit-C 처리에서 높은 값을 나타내었다. 색도는 전반적으로 건조가 진행될수록 밝기를 나타내는 L 값은 감소하는 경향을 보였으며 색차는 Vit-C>NaCl>Sol-Ca>non-treated 순으로 낮은 값을 나타내었다. 갈변도 또한 색차의 결과와 일치하는 경향을 나타내었다. 총당은 무처리, NaCl, Vit-C 및 Sol-Ca 처리구가 무처리에 비해 높은 함량을 나타내어 향기 및 감미 유지 효과가 있는 것으로 관찰되었고, 유리당은 Vit-C 및 Sol-Ca 처리구에서 fructose, glucose 및 sucrose의 함량이 높은 것으로 나타났다. 절단력은 무처리에 비해 전처리구에서 증가하는 결과를 나타내었으며, Sol-Ca 처리구에서 가장 높은 값을 나타내었다. 관능특성에서는 전처리 구가 무처리에 비하여 외관, 색, 맛, 종합적 기호도가 높아지는 결과를 나타내었으며 특히, Vit-C 처리에서 가장 높은 기호특성을 나타내었다. 전처리방법에 따른 폴리페놀, 플라보노이드 및 프로안토시아니딘 함량에서는 전반적으로 전처리 구에서 높은 함량을 나타내었으며 프로안토시아니딘 함량에서 Vit-C 처리에서 가장 높은 함량을 나타내었다. 항산화능에서는 유용성분 함량이 높을수록 라디칼 소거능이 높아지는 양적 상관관계를 나타내었다.

• Journal of Plant Biotechnology
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• 제30권4호
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• pp.369-373
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• 2003

In spite of potential benefits of anther culture, low productivity of plant regeneration in some genotypes; e.g. tonsil and indica rice, is one of the major obstacles for practical use of anther culture. This study was conducted to improve cold shock method and carbohydrate source for increasing the efficiency of anther culture in rice. The most common carbon source, sucrose was replaced to maltose, which has two molecules of glucose. Maltose increased callus induction 1.4-to 1.8-fold higher in japonica rice, 3.2-to 11.6-fold in tongil types and 2.7-fold in indica rice IR50. Callus induction was increased from 0.2％ to 12.5％ in maltose medium compared to the medium supplemented with sucrose plus glucose in indica rice "Tetep". A simple procedure of vacuum packaging of panicles during cold shock treatment prolonged not only anther viability more than 15 days but also increased callus induction more than 2-fold compared to open-air storage (conventional method). Combining of above two methods, callus induction was increased 28 to 56％ in japonica, 13 to 33％ in tonsil type and 12 to 31％ in indica rice. Plant regeneration was increased 14 to 35％ in japonica, 10 to 20％ in tonsil and 4 to 15％ in indica rice, respectively.