Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Supercooling Pretreatment Improves the Shelf-Life of Freeze-Dried Leuconostoc mesenteroides WiKim32

  • Seul-Gi Jeong (Technology Innovation Research Division, World Institute of Kimchi) ;
  • In Seong Choi (Technology Innovation Research Division, World Institute of Kimchi) ;
  • Ho Myeong Kim (Technology Innovation Research Division, World Institute of Kimchi) ;
  • Ji Yoon Chang (Technology Innovation Research Division, World Institute of Kimchi) ;
  • Hae Woong Park (Technology Innovation Research Division, World Institute of Kimchi)
  • Received : 2022.09.14
  • Accepted : 2022.10.21
  • Published : 2022.12.28
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Abstract

Storage stability of freeze-dried lactic acid bacteria is a critical factor for their cost-effectiveness. Long-term storage of lactic acid bacteria enables microbial industry to reduce distribution costs. Herein, we investigated the effect of cold adaptation under supercooling conditions at -5℃ on the viability of Leuconostoc mesenteroides WiKim32 during the freeze-drying process and subsequent storage. Cold adaptation increased the thickness of exopolysaccharides (EPS) and improved the viability of freeze-dried Leu. mesenteroides WiKim32. Compared to non-adapted cells, cold-adapted cells showed a 35.4% increase in EPS thickness under supercooling conditions. The viability of EPS-hydrolyzed cells was lower than that of untreated cells, implying that EPS plays a role in protection during the freeze-drying process. Cold adaptation increased the storage stability of freeze-dried Leu. mesenteroides WiKim32. Fifty-six days after storage, the highest viability (71.3%) was achieved with cold adaptation at -5℃. When EPS-containing broth was added prior to the freeze-drying process, the viability further increased to 82.7%. These results imply that cold adaptation by supercooling pretreatment would be a good strategy for the long-term storage of Leu. mesenteroides WiKim32.

Keywords

Acknowledgement

This research was supported by World Institute of Kimchi (Grant No. KE2202-1-2), funded by Ministry of Science and ICT, Republic of Korea, and by the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry (IPET) through Agricultural Machinery/Equipment Localization Technology Development Program, funded by Ministry of Agriculture, Food and Rural Affairs (MAFRA) (Grant No. 321054-05).

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