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Determination of Aluminum Leached from Cooking Utensils by using Flow Injection Analysis (흐름주입분석법에 의한 주방용기로부터 용출된 알루미늄의 정량)

  • Choi, Yong Wook
    • Journal of Korean Society on Water Environment
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    • v.20 no.3
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    • pp.281-289
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    • 2004
  • Optimal analytical conditions have been established for mixing and reaction coil length, concentrations of ECR, CTAB, and acetate, reaction temperature and pH in the determination of aluminum by using flow injection analysis(FIA). Detection limit and repeatability for this system were $3{\mu}g/L$ and better than 1% of relative standard deviation, respectively. The interference from iron(III) was suppressed up to 6mg/L of iron(III) by $0.1{\mu}M$ ascorbic acid, and the interference from fluoride could be tolerated in absorption of Al/ECR/CTAB derivative by 10mM boric acid at pH 3.5 adjusted with nitric acid. This FIA system was applied to the determination of aluminum leached from cooking utensils by distilled and tap water containing fluoride ranged from 0.5 to 2mg/L by measurement of absorbance for Al/ECR/CTAB at 585nm. The leached aluminum content was increased with increasing concentration of fluoride. The aluminum contents leached from aluminum utensils by tap water were about 9 fold higher than those leached by distilled water, whereas aluminum contents leached by distilled water containing fluoride were about 2 fold higher compared to those leached by distilled water. That represented that other dissolved substances as well as fluoride in tap water contributed to liberation of aluminum from aluminum utensils. A comparison between FIA data and ICP-AES data by correlation and paired t test showed that the FIA system could be accepted as a good alternative method for the determination of aluminum in tap water.

Cloning and Expression Characteristics of Pharbitis nil COP1 (PnCOP1) During the Floral Induction

  • Kim, Yun-Hee;Kim, Seong-Ryong;Heo, Yoon-Kang
    • Journal of Photoscience
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    • v.12 no.1
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    • pp.1-9
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    • 2005
  • The ubiquitin E3 ligase COP1 (Constitutive Photomorphogenesis 1) is a protein repressor of photomorphogenesis in Arabidopsisplants, and it found in various organisms, including animals. The COP1 protein regulates the stability of many of the light-signaling components that are involved in photomorphogenesis and in the developmental processes. To study the effect of COP1 on flowering in a short day plant, we have cloned a full-length of PnCOP1 (Pharbitis nil COP1) cDNA from Pharbitis nil Choisy cv. Violet, and we examined its transcript levels under various conditions. A full-length PnCOP1 cDNA consists of 2,280 bp nucleotidesthat contain 47 bp of 5'-UTR, 232 bp of 3'-UTR including the poly (A) tail, and 1,998 bp of the coding sequence. The deduced amino acid sequence contains 666 amino acids, giving it a theoretical molecular weight of 75 kD and a isolectric point of 6.2. The PnCOP1 contains three distinct domains, an N-terminal $Zn^2+$-binding RING-finger domain, a coiled-coil structure, and WD40 repeats at the C-terminal, implying that the protein plays a role in protein-protein interactions. The PnCOP1 transcript was detected in the cotyledon, hypocotyls and leaves, but not in root. The levels of the PnCOP1 transcript were reduced in leaves that were a farther distance away from the cotyledons. The expression level of the PnCOP1 gene was inhibited by light, while the expression was increased in the dark. During the floral inductive 16 hour-dark period for Pharbitis nil, the expression was increased and it reached its maximum at the 12th hour of the dark period. The levels of PnCOP1 mRNA were dramatically reduced upon light illumination. These results suggest that PnCOP1 may play an important function in the floral induction of Pharbitis nil.

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A Study on the Non-Contact Detection Technique of Defects Using AC Current - The Influence of Frequency and lift-off - (교류전류를 이용한 비접촉결함탐상법에 관한 연구 - 주파수 lift-off의 영향 -)

  • Kim, Hoon;Na, Eu-Gyun
    • Journal of the Korean Society for Nondestructive Testing
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    • v.22 no.1
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    • pp.53-58
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    • 2002
  • New nondestructive inspection (NDI) technique to detect the defect in metal was developed in which an electromagnetic field is induced in a metal by AC current flowing in the magnetic coil and the leak magnetic-flux disturbed by defects is measured using a tape-recorder head with air gap. This technique can be applied in evaluating the location and sizing of surface defects in components of the ferromagnetic body by means of the non-contacting measurement. In this paper, we have applied this technique to the evaluation of two-dimensional surface cracks in ferromagnetic metal, and also investigated the influence of the various frequencies and lift-off. Defects were detected with maximum values in the distribution of voltage and it was found that the maximum values tend to increase with the defect depth. Although the maximum values for defects are affected by the frequency and lift-off, the depth of small defects can be estimated from the linear relationship between the depth and voltage rate$(V_0/V_{ave})$.

Analysis of Structure and Expression of Grapevine 2-oxoglutarate Oxygenase Genes in Response to Low Temperature

  • Kim, Seon Ae;Ahn, Soon Young;Yun, Hae Keun
    • Horticultural Science & Technology
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    • v.34 no.1
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    • pp.46-54
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    • 2016
  • 2-Oxoglutarate (2OG) acts as a signaling molecule and plays a critical role in secondary metabolism in a variety of organisms, including plants. Six 2-oxoglutarate (2OG) and Fe(II) oxygenase (2OGO) genes, VlCE2OGO1 [Vitis labruscana 2-oxoglutarate (2OG) and Fe(II) oxygenase 1], VlCE2OGO2, VlCE2OGO3, VlCE2OGO4, VlCE2OGO5, and VlCE2OGO6, which show different expression patterns upon transcriptome analysis of 'Campbell Early' grapevine exposed to low temperature for 4 weeks, were analyzed for their structure and expression. Comparison of the deduced amino acid sequences of the 2OGO genes from the V. labruscana transcripts revealed sequence similarities of 38.6% (VlCE2OGO1 and VlCE2OGO2) to 19.2% (VlCE2OGO2 and VlCE2OGO3). The lengths of these genes ranged from 1053 to 2298 bp, and they encoded 316 to 380 amino acids. The prediction of the secondary structure of the encoded proteins by Self-Optimized Prediction Method with Alignment (SOPMA) indicated that all the genes contained alpha helix (23.95 to 41.71%), extended strand (16 to 22.34%), beta turn (6.65 to 9.22%), and random coil (32.97 to 51.58%) in the analysis. Specific primers from unique regions in each gene obtained by alignment of nucleotide sequences were used in real time PCR for analysis of gene expression. All tested genes showed differential expression in grapevines exposed to low temperature. Of the six transcripts, VlCE2OGO1, VlCE2OGO2, and VlCE2OGO3 were up-regulated and VlCE2OGO4, VlCE2OGO5, and VlCE2OGO6 were down-regulated in response to cold treatments at all tested time points. The 2OG genes can be used for elucidation of mechanisms of tolerance to cold and as valuable molecular genetic resources for selection in breeding programs for cold-hardy grapevines.

A Study on the Development of Standardization Tester for the Field Inspection of the Rate of Rise Spot Type Heat Detector (차동식스포트형열감지기 현장점검용 표준화기기 개발에 관한 연구)

  • Kim, Shi-Kuk;Yuk, Hyun-Dai;Yang, Seung-Hyun;Jee, Seung-Wook;Lee, Chun-Ha
    • Fire Science and Engineering
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    • v.23 no.3
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    • pp.85-92
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    • 2009
  • This paper studied on the development of standardization tester for the field inspection of the rate of rise spot type heat detector. It was to find the problems of the 4 type's testers such as A type (electric bulb type), B type (halogen lamp type), C type (heating coil type), D type (fumigator type) which were used to check of the rate of rise spot type heat detector in inspection for the fire protection. To identify those problems, this paper described about the development of standardization tester for the field inspection of the rate of rise spot type heat detector and that have the function of auto setting temperature more $30^{\circ}C$ higher than room temperature and keep the constant temperature. And keep the constant wind velocity of 85cm/sec. Performance verification results of the developed prototype that had same conditions such as the sensitivity test conditions at the type approval of the rate of rise spot type heat detector.

Compensation of the Discontinuous Properties of the Guide System using Magnetic Levitation (자기 부상 안내 기구의 불연속 특성 보상 방법)

  • Lee, Sang Joon;Jung, Kwang Suk
    • Journal of Institute of Convergence Technology
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    • v.3 no.2
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    • pp.11-15
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    • 2013
  • These days, the quality of goods is required to improve in the process of manufacturing the semiconductor through the short working hours and clean transportation. The non-contact transport device using magnetic levitation can be a solution in the manufacturing process. The non-contact transport device, using electromagnetic actuation, is the system that can actually transport them by only using attraction force from the electromagnetic source without authentic contact. Moreover, the system using electromagnetic force has a substantial number of benefits ranging from unrestricted design to unlimited expansion. Especially, the price is competitive. The non-contact transport device, using electromagnetic force, has another merits in controlling by giving the same amount of attraction force to ferromagnetic body. By controlling the currents given to coil, the operator is able to decide the direction of the transportation. In order to design the optimal system, we implemented five different things such as the presence of the links below the electromagnetic, the electromagnet changes due to coupling method, the change according to the thickness of the links below electromagnet, due to changes in between electromagnetic distance direction, and the size of the current. Through simulations and the optimum design, it seems to control easily and figure out the exact size of power. It might definitely be the non-contact transport that can sharply reduce tiny scratches and particles in the process of manufacturing the semiconductor.

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A Study for Detecting a Gazing Point Based on Reference Points (참조점을 이용한 응시점 추출에 관한 연구)

  • Kim, S.I.;Lim, J.H.;Cho, J.M.;Kim, S.H.;Nam, T.W.
    • Journal of Biomedical Engineering Research
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    • v.27 no.5
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    • pp.250-259
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    • 2006
  • The information of eye movement is used in various fields such as psychology, ophthalmology, physiology, rehabilitation medicine, web design, HMI(human-machine interface), and so on. Various devices to detect the eye movement have been developed but they are too expensive. The general methods of eye movement tracking are EOG(electro-oculograph), Purkinje image tracker, scleral search coil technique, and video-oculograph(VOG). The purpose of this study is to embody the algorithm which tracks the location of the gazing point at a pupil. Two kinds of location data were compared to track the gazing point. One is the reference points(infrared LEDs) which is effected from the globe. Another is the center point of the pupil which is gained with a CCD camera. The reference point was captured with the CCD camera and infrared lights which were not recognized by human eyes. Both of images which were thrown and were not thrown an infrared light on the globe were captured and saved. The reflected reference points were detected with the brightness difference between the two saved images. In conclusion, the circumcenter theory of a triangle was used to look for the center of the pupil. The location of the gazing point was relatively indicated with the each center of the pupil and the reference point.

Frequency Characteristics of 2-Layer Spiral Planar Inductor (2층 나선형 박막 인덕터의 주파수 특성)

  • Kim, Jae-Wook;Ryu, Chang-Keun
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.12 no.9
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    • pp.4101-4106
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    • 2011
  • In this study, we propose that the structures of 2-layer spiral planar inductors have a lower spiral coil and via increasing inductance in limited possession are and confirm the frequency characteristics. The structures of inductors have Si thickness of $300{\mu}m$, $SiO_2$ thickness of $7{\mu}m$. The width of Cu coils and the space between segments have $20{\mu}m$, respectively. The number of turns of coils have 3. The performance of spiral planar inductors was simulated to frequency characteristics for inductance, quality-factor, SRF(Self- Resonance Frequency) using HFSS. The 2-layer spiral planar inductors have inductance of 3.2nH over the frequency range of 0.8 to 1.8 GHz, quality-factor of maximum 8.2 at 2.5 GHz, SRF of 5.8 GHz. Otherwise, 1-layer spiral planar inductors have inductance of 1.5nH over the frequency range of 0.8 to 1.8 GHz, quality-factor of maximum 18 at 8 GHz, SRF of 19.2 GHz.

Usefulness of Dexmedetomidine during Intracerebral Aneurysm Coiling

  • Lee, Hyoun-Ho;Jung, Young-Jin;Choi, Byung-Yon;Chang, Chul-Hoon
    • Journal of Korean Neurosurgical Society
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    • v.55 no.4
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    • pp.185-189
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    • 2014
  • Objective : General anesthesia is often preferred for endovascular coiling of intracranial aneurysm at most centers. But in the authors' hospital, it is performed under monitored anesthesia care (MAC) using dexmedetomidine. To determine the feasibility and safety of this approach, the authors reviewed our initial experience. Methods : Retrospective data was analyzed from July 2012 to November 2012. We performed coil embolization in 28 cases using this method. Among them, for statistical significance, we analyzed 12 cases in which the procedure time exceeded an hour. Vital signs were analyzed every 10 minutes. Depth of sedation was measured according to the Ramsay sedation scale and frequency of the repeated roadmap image(s) caused by movement of the patient's head during the procedure. Results : All procedures were completed without occurrence of procedure related complications. Under MAC using dexmedetomidine, vital signs of the patients were stable, no statistical significance regarding hemodynamic and respiratory parameters was observed between time points (p>0.05). Adequate sedation was achieved. Mean Ramsay sedation scale was $3.67{\pm}1.61$ (2 to 6). Repeated roadmap image(s) due to patient's factor occurred in only one case. The mean dosage of drug for adequate sedation for the procedure was $0.65{\pm}0.12mcg/kg/hr$ without loading doses. Conclusion : To the best of my knowledge, this is the first report published in English using the method of monitored anesthesia with dexmedetomidine for intracranial aneurysm coiling. Monitored anesthesia care using dexmedetomidine without loading dose for embolization of intracranial aneurysms appeared to be a safe and effective alternative to general anesthesia.

Reappraisal of Anatomic Outcome Scales of Coiled Intracranial Aneurysms in the Prediction of Recanalization

  • Lee, Jong Young;Kwon, Bae Ju;Cho, Young Dae;Kang, Hyun-Seung;Han, Moon Hee
    • Journal of Korean Neurosurgical Society
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    • v.53 no.6
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    • pp.342-348
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    • 2013
  • Objective : Several scales are currently used to assess occlusion rates of coiled cerebral aneurysms. This study compared these scales as predictors of recanalization. Methods : Clinical data of 827 patients harboring 901 aneurysms treated by coiling were retrospectively reviewed. Occlusion rates were assessed using angiographic grading scale (AGS), two-dimensional percent occlusion (2DPO), and volumetric packing density (vPD). Every scale had 3 categories. Followed patients were dichotomized into either presence or absence of recanalization. Kaplan-Meier analysis was conducted, and Cox proportional hazards analysis was performed to identify surviving probabilities of recanalization. Lastly, the predictive accuracies of three different scales were measured via Harrell's C index. Results : The cumulative risk of recanalization was 7% at 12-month, 10% at 24-month, and 13% at 36-month of postembolization, and significantly higher for the second and third categories of every scale (p<0.001). Multivariate-adjusted hazard ratios (HRs) of the second and third categories as compared with the first category of AGS (HR : 3.95 and 4.15, p=0.004 and 0.001) and 2DPO (HR : 4.87 and 3.12, p<0.001 and 0.01) were similar. For vPD, there was no association between occlusion rates and recanalization. The validated and optimism-adjusted C-indices were 0.50 [confidence (CI) : -1.09-2.09], 0.47 (CI : -1.10-2.09) and 0.44 (CI : -1.10-2.08) for AGS, 2DPO, and vPD, respectively. Conclusion : Total occlusion should be reasonably tried in coiling to maximize the benefit of the treatment. AGS may be the best to predict recanalization, whereas vPD should not be used alone.