• Journal of the Earthquake Engineering Society of Korea
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• v.11 no.3 s.55
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• pp.63-72
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• 2007

The Emergency Diesel Generator (EDG) is a very important equipment for the safety of a Nuclear Power Plant (NPP). In this study, the operating vibration of three kinds of EDG systems was measured. The target EDG systems are Yonggwang 5 unit and Ulchin 2 and 3 units. The Yonggwang 5 and Ulchin 3 unit EDG systems are the same type but the foundation systems are different. One is an anchor bolt type and the other is a spring and viscous-damper type. The purpose of these measurements is for a verification of the vibration isolation effect depending on the foundation system. As a result, It can be said that the spring and viscous damper system of the EDG performed better for the vibration isolation than that of anchor bolt type.

• Particle and aerosol research
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• v.13 no.2
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• pp.65-77
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• 2017

In recent large-scale semiconductor manufacturing cleanrooms, the energy consumption in outdoor air conditioning (OAC) systems to heat, humidify, cool and dehumidify outdoor air(OA) represents about 40~50 % of the total cleanroom power consumption required to maintain cleanroom environment. Therefore, the assessment of energy consumption in outdoor air conditioning systems is essential for reducing the outdoor air conditioning load for a cleanroom. In the present study, an experiment with an outdoor air flow rate of $1,000m^3/h$ was conducted to compare the energy consumption in steam humidification, simple air washer, exhaust air heat recovery type air washer and dry cooling coil(DCC) return water heat recovery type air washer OAC systems. Besides, a numerical analysis was carried out to evaluate the annual energy consumption of the aforementioned four OAC systems. It was shown that the simple air washer, exhaust air heat recovery type air washer and DCC return water heat recovery type air washer OAC systems using water spray humidification were more energy-efficient than the steam humidification OAC system. Furthermore the DCC return water heat recovery type air washer OAC system was the most energy-efficient.

• The Journal of the Acoustical Society of Korea
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• v.22 no.5
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• pp.337-344
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• 2003

In DC/DC converter and VVVF inverter, which are the must dominant noise sources of Maglev train, noise is radiated from core and coil excited by MS(Magnetostriction). The main noise source of DC/DC converter is transformer whose spectrum shows strong peaks associated with harmonics of exciting frequency, On the other hand, LIM/VVVF noise is dominated by the harmonics of switching frequency, whereas harmonics of exciting frequency are not significant. As switching frequency is increased in VVVF inverter, it is shown that the harmonics are shifted to higher frequency range. If switching frequency is increased from 700㎐ to 2 ㎑, It is measured that noise can be reduced by 5 to 6 ㏈. Since complete mathematical description of MS phenomena is far beyond the present technology, vibration spectrum is investigated qualitatively in this paper, where effect of increasing switching frequency is confirmed.

• Korean Journal of Food Science and Technology
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• v.31 no.6
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• pp.1488-1494
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• 1999

Effect of ${\gamma}-irradiation$ on the SDS-PAGE pattern, secondary structure content, the solubility of commercial soy protein isolate (SPI) and whey protein concentrate (WPC) was investigated. The change in the subunit molecular weight of SPI and WPC irradiated in aqueous solution or dried state was studied using SDS-PAGE. The SDS-PAGE pattern of SPI irradiated in aqueous solution revealed the fragmentation and aggregation of the subunit protein. For WPC irradiated in aqueous solution. fragmentation of the subunit protein up to 10 kGy was observed. In contrast, ${\gamma}-irradiation$ of SPI and WPC in dried state did not cause any significant changes in the SDS-PAGE pattern. The change In the secondary structure of irradiated SPI and WPC solution was studied using circular dichroism. The aperiodic structure content of SPI and WPC solution increased at higher irradiation doses, which suggests that ${\gamma}-irradiation$ caused the disruption of the ordered structure of SPI and WPC solution. Gamma-irradiation also caused the change in solubility of SPI and WPC in dried state.

• Molecules and Cells
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• v.39 no.6
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• pp.495-500
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• 2016

We have solved the crystal structure of a predicted fructose-specific enzyme $IIB^{fruc}$ from Escherichia coli ($EcEIIB^{fruc}$) involved in the phosphoenolpyruvate-carbohydrate phosphotransferase system transferring carbohydrates across the cytoplasmic membrane. $EcEIIB^{fruc}$ belongs to a sequence family with more than 5,000 sequence homologues with 25-99% amino-acid sequence identity. It reveals a conventional Rossmann-like ${\alpha}-{\beta}-{\alpha}$ sandwich fold with a unique ${\beta}$-sheet topology. Its C-terminus is longer than its closest relatives and forms an additional ${\beta}$-strand whereas the shorter C-terminus is random coil in the relatives. Interestingly, its core structure is similar to that of enzyme $IIB^{cellobiose}$ from E. coli ($EcIIB^{cel}$) transferring a phosphate moiety. In the active site of the closest $EcEIIB^{fruc}$ homologues, a unique motif CXXGXAHT comprising a P-loop like architecture including a histidine residue is found. The conserved cysteine on this loop may be deprotonated to act as a nucleophile similar to that of $EcIIB^{cel}$. The conserved histidine residue is presumed to bind the negatively charged phosphate. Therefore, we propose that the catalytic mechanism of $EcEIIB^{fruc}$ is similar to that of $EcIIB^{cel}$ transferring phosphoryl moiety to a specific carbohydrate.

• Journal of Microbiology
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• v.40 no.2
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• pp.156-160
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• 2002

The whole gal/lae operon genes of Lactococcus lactis ssp. lactis 7962 were reported as follows: galA-galM-galK-galT-lacA -lacZ-galE. The galK gene encoding a galactokinase involved in one of the Leloir pathways for galactose metabolism was found to be 1,197 bp in length and encodes a protein of 43,822 Da calculated molecular mass. The deduced amino acid sequence showed over 50% homology with GaIK proteins from several other lactic acid bacteria. The galK gene was expressed in E. coli and the product was identified as a 43 kDa protein which corresponds to the estimated size from the DNA sequence. The galactokinase activity of recombinant 5. coli was about 8 times greater against that of the host strain and more than 3 times higher than the induced L. lactis 7962.

• KSBB Journal
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• v.21 no.2
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• pp.129-132
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• 2006

The importance of natural preservative has increased in recent years. The natural preservatives have been used in the field of foods, cosmetics and pharmacology. In the present work Artemisia sp., well recognized for their effect of antimicrobial activity, were extracted by methanol and water sequentially for selecting only water-soluble compounds that can be used as additives in food and cosmetics. Antimicrobial activities of water extracts from stem and leaf of Artemisia princeps var. orientalis were investigated by the disc diffusion method. Two gram positive bacteria(Staphylococcus aureus and Bacillus subtilis) and three gram negative bacteria(Escherichia coil, Agrobacterium tumefaciens and Pseudomonas putida) were used for antimicrobial activity studies. The water-soluble compounds from methanol extract showed higher antimicrobial activity than only water extract to these bacteria. Comparative evaluation of water-soluble metabolite profiles with caffeic acid that is known as an antimicrobial compound from Artemisia sp. was performed by high performance liquid chromatography with photo-diode array detection.

• KIEAE Journal
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• v.16 no.4
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• pp.41-46
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• 2016

Purpose: This is a study on the planning of temporary housing for post application of Mega Sports facilities. The subject of the study is 2018 Pyeongchang Winter Olympics, which is to suggest building an alternative temporary housing using shipping containers(high cube), which solve the lack of accommodations and recycle temporary housing after Olympics, save money and be eco-friendly in Olympics. Method: This study includes this ; research on the a fact-finding survey about Mega sports facilities post application and demand survey on 2018 Pyeongchang Winter Olympics accomodations and an analysis about temporary housing plan. Furthermore we decided temporary housing building plan by analyzing residents' needs and traits of the housing etc. Through this, we made a schematic design for household units. Result: As a result, this study is a plan of making space, forms, and structure. The planned size is $38.4m^2$(L:12m, W:3.2m) except balcony, and indoor height is 2.5m. The space consists of entrance, bathroom, bedroom and living room with folding furniture system. Also there's a detailed floor plan of the ceiling, wall, and floor we drew up. The ceiling and wall consist of dampproof film, noncombustible board, fire proof urethane form, and color-designed sheet. The floor is composed of floor tile, cement mortar, light concrete(with heat coil), insulation, and dampproof film. Additionally, this study is a plan of interior dry wall with detail using modular construction method for work efficiency and quality improvement.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.12 no.1
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• pp.84-91
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• 2001

In in vitro cell experiments for the biological assessment of electromagnetic (EM) field, exposure system (ES) must be analyzed in terms of current density (J) and induced electric field intensity (E). Although in uniform B field, E and J in the sample medium are not distributed uniformly because of conductivity in sample dish. Thus, the precise estimation for E and J induced by uniform ELF within sample media is very important keys for successful in in vitro experiments. In this paper, we designed in vitro ELF ES with electromagnetic analysis using MATLAB simulator. Then we calculated from the measured B field to verify induced E & J distribution for random locations of cells within media in two cases of samples existence or not. ES with B field ranging from 0 to 20G consists of Helmholz coil and current generator based on the microprocessor. Also, we developed ELFES for each B field generation as uniform and non-uniform modes.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.41 no.4
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• pp.333-336
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• 2017

A speaker diaphragm generates a divided vibration. The influence of the break-up mode is sufficient to cause a shape change in the diaphragm. In this paper, is widely used in ultra-thin multi-media devices, including smart phones is the advance guard of the IT sector, the micro-speakers and its target. Micro-speakers are different from general speakers. The plate has structural form and space constraints. In particular, they utilize a closed-type drive space. It is difficult to provide cooling for the auxiliary suspension structure because of the heat generated in the moving coil. The present study considered the relationship between the break-up mode and the heat transfer of the diaphragm. An experiment was conducted in two stages to compare the embodiment of the break-up mode and heat transfer in a certain frequency range. The changes in the heat were determined through measurements and thermal imaging of the break-up mode. The break-up mode tendency of the diaphragm could be rapidly predicted based on the imaging results using the thermal imaging camera. This will help in the optimal design of micro-speakers.