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Expression of the Galactokinase Gene (gaIK) from Lactococcus lactis asp. lactis ATCC7962 in Escherichia coil  

Lee, Hyong-Joo (Department of Food Science and Technology, Seoul National University, Suwon 441-774)
Lee, Jung-Min (Department of Food Science and Technology, Seoul National University, Suwon 441-774)
Park, Jae-Yeon (Department of Food Science and Biotechnology, Kyonggi University, Suwon 442-760)
Lee, Jong-Hoon (Department of Food Science and Biotechnology, Kyonggi University, Suwon 442-760)
Kim, Jeong-Hwon (Department of food Science and Technology, Gyeongsang National University, Chinju 660-701)
Chang, Hea-Choon (Department of Food Nutrition, Chosun University, Kwamgiu 501-701)
Chung, Dae-Kyun (Institute and Department of Genetic Engineering, Kyunghee University, Suwon 449-701)
Kim, Somi-Cho (Subtropical Horticulture Research Center, Cheju National University, Cheju 690-756)
Publication Information
Journal of Microbiology / v.40, no.2, 2002 , pp. 156-160 More about this Journal
Abstract
The whole gal/lae operon genes of Lactococcus lactis ssp. lactis 7962 were reported as follows: galA-galM-galK-galT-lacA -lacZ-galE. The galK gene encoding a galactokinase involved in one of the Leloir pathways for galactose metabolism was found to be 1,197 bp in length and encodes a protein of 43,822 Da calculated molecular mass. The deduced amino acid sequence showed over 50% homology with GaIK proteins from several other lactic acid bacteria. The galK gene was expressed in E. coli and the product was identified as a 43 kDa protein which corresponds to the estimated size from the DNA sequence. The galactokinase activity of recombinant 5. coli was about 8 times greater against that of the host strain and more than 3 times higher than the induced L. lactis 7962.
Keywords
Orientia tsutsugamushi; superoxide dismutase; gene cloning; PCR; intracellular survival;
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