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Development and Validation of an Analytical Method for Ametoctradin Residue Determination in Domestic Agricultural Commodities by HPLC-PDA (HPLC-PDA를 이용한 국내 유통 농산물 중 ametoctradin 잔류량 분석법 개발 및 검증)

  • Do, Jung-Ah;Kwon, Ji-Eun;Lee, Eun-Mi;Kim, Mi-Ra;Kuk, Ju-Hee;Cho, Yoon-Jae;Kang, Il-Hyun;Kim, Hyung-Su;Kwon, Kisung;Oh, Jae-Ho
    • Korean Journal of Food Science and Technology
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    • v.45 no.3
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    • pp.285-292
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    • 2013
  • This study was carried out to validate the safety of ametoctradin residues in agricultural commodities by developing an official analysis method. An analytical method was developed and validated using HPLC-PDA detectors. The samples were extracted with methanol, subsequently partitioned with dichloromethane and purified with florisil column chromatograph using acetone/hexane (30/70, v/v) as solvent. The method was validated by using grape, hulled rice, mandarin, and potato spiked with ametoctradin at 0.05 and 5.0 mg/kg, and pepper at 0.05 and 2.0 mg/kg. Average recoveries were 76-114.8% with relative standard deviation less than 10%, and the limit of detection and limit of quantification were 0.0125 and 0.05 mg/kg, respectively. The result of recoveries and overall coefficient of variation of the laboratory results from Gwangju regional Food and Drug Administration (FDA) and Daejeon regional FDA was accorded with Codex Alimentarius Commission Guideline (CAC/GL 40). Based on these results, this method was found to be appropriate for ametoctradin residue determination and can be used as the official method of analysis.

A study on determination of the lime requirement based on exchangeable aluminum content (치환성(置換性) Al 함량(含量)에 따른 석탄소요량(石炭所要量) 결정(決定)에 관(關)한 연구(硏究))

  • Ryu, In Soo;Cho, Seong Jin;Yuk, Chang Soo
    • Korean Journal of Soil Science and Fertilizer
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    • v.7 no.3
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    • pp.185-191
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    • 1974
  • Incubation and pot studies were conducted with upland soils for a study on determination of the lime requirement based on exchangeable alumium content. The results obtained are as follows; 1. Results of chemical analysis of upland soils show that pH varies from 5.0 to 5.4, and exchangeable Al moves with the range of 1.3-3.0m.e/100gr. Exchangeable Al decreases with years of cultivation. 2. Incubation studies shows that on acid mineral soils almost all exchangeable Al, on average 95% was neutralized with the lime to neutralized 100% exchangeable Al. On volcanic ash soil, however, only 65.5% was neutralized with the lime estimated to neutralize the equivalent of 200% exchangeable Al. The latter has required more lime. 3. The pH of mineral soils is on the average increased from an initial 5.2 to 6.3 when 95% of exchangeable Al is neutralized, whereas that on volcanic ash soil is increased from an initial 5.3 to 5.5 only when lime is applied at rate to neutralize the equivalent of 200% exchangeable Al. 4. A high correlation coefficient (r=0.99) was obtained between exchangeable Al and exchangeable acidity. This indicates that exchangeable acidity is primarly a result of exchangeable Al. 5. In pot experiments with soybean cultivated on one of the hill land soils (Songjoong soil) the application of fused phosphate and triple superphosphate based on a 5% saturation rate ($P_2O_5$ 32.1 kg/10a) showed that the liming factor for calculation of the optimum lime requirements based on exchangeable acidity was 0.594 for fuses phosphate or 1.132 for tripple superphosphate, and optimum pH is approximately 6.0 and optimum neutralization rate of exchangeable Al is 80-90%.

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Development of Porcine Pericardial Heterograft for Clinical Application (Tensile Strength-thickness) (돼지의 심낭을 이용한 이종이식 보철편의 개발 (장력-두께간의 구조적 특성))

  • Kim, Kwan-Chang;Lee, Cheul;Choi, Chang-Hue;Lee, Chang-Ha;Oh, Sam-Sae;Park, Seong-Sik;Kim, Kyung-Hwan;Kim, Woong-Han;Kim, Yong-Jin
    • Journal of Chest Surgery
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    • v.41 no.2
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    • pp.170-176
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    • 2008
  • Background: Bioprosthetic devices for treating cardiovascular diseases and defects may provide alternatives to autologous and homograft tissue. We evaluated the mechanical and physical conditions of a porcine pericardial bioprosthesis treated with Glutaraldehyde (GA), Ethanol, or Sodium dodecylsulfate (SDS) before implantation. Material and Method: 1) Thirty square-shaped pieces of porcine pericardium were fixed in 0.625%, 1.5% or 3% GA solution. 2) The tensile strength and thickness of these and other bioprosthesis, including fresh porcine pericardium, fresh human pericardium, and commercially produced heterografts, were measured. 3) The tensile strength and thickness of the six treated groups (GA-Ethanol, Ethanol-GA, SDS only, SDS-GA, Ethanol-SDS-GA and SDS-Ethanol-GA) were measured. Result: 1) Porcine pericardium fixed in 0.625% GA the thinnest and had the lowest tensile strength, with thickness and tensile strength increasing with the concentration of GA solution. The relationship between tensile strength and thickness of porcine pericardium increased at thicknesses greater than 0.1mm (correlation-coefficient 0.514, 0<0.001). 2) There were no differences in tensile strength or thickness between commercially-produced heterografts. 3) Treatment of GA, ethanol, or SDS minimally influenced thickness and tensile strength of porcine pericardium, except for SDS alone. Conclusion: Porcine pericardial bioprosthesis greater than 0.1 mm thick provide better handling and advantageous tensile strength. GA fixation did not cause physical or mechanical damage during anticalcification or decellularization treatment, but combining SDS-ethanol pre-treatment and GA fixation provided the best tensile strength and thickness.

Length-Weight Relations and Condition Factor (K) of Zacco platypus Along Trophic Gradients in Reservoir Ecosystems (인공호의 부영양화에 따른 피라미(Zacco platypus) 개체군의 전장-체중 관계 및 비만도 지수)

  • Ko, Dae-Geun;Han, Jeong-Ho;An, Kwang-Guk
    • Korean Journal of Ecology and Environment
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    • v.45 no.2
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    • pp.174-189
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    • 2012
  • The objective of this study was to determine the weight-length relations and condition factor (K) of Zacco platypus, along the trophic gradients from oligotrophic to eutrophic state in six reservoir ecosystems ($B_aR$, $Y_yR$, $J_yR$, $G_pR$, $Y_dR$, and $M_sR$), during 2008~2010. The species was selected as a sentinel species for the study, due to its wide distribution and wide trophic gradient. The analysis of trophic state index (TSI), based on total phosphorus (TP) and chlorophyll-a (Chl-a), indicated that reservoirs of $Y_yR$ and $B_aR$ were classified as to be in an oligotrophic state (30~40), the $J_yR$ and $G_pR$ as mesotrophic (40~50), and the $Y_dR$ and $M_sR$ as eutrophic state (50~70). Total 47 species and 26,226 individuals were sampled from 6 reservoirs and sensitive species dominated in the oligotrophic reservoirs ($Y_yR$ and $B_aR$). In the mean time, the tolerant speciesdominated the community in the mesotrophic ($J_yR$ and $G_pR$) and eutrophic ($Y_dR$ and $M_sR$) reservoirs. Regression analysis of body weight, against the total length, indicated that the regression coefficient (b value) was lower in the oligotrophic reservoir (2.77~2.79) than the mesotrophic (3.07~3.17) and eutrophic reservoirs (3.15~ 3.21). This result suggests that the population growth rate Zacco platypus reflected the trophic gradients of the reservoirs. The analysis of condition factor (K) against the total length showed positive slopes (b>3.0) in mesotrophic and eutrophic reservoirs, and a negative slope (b<3.0) in oligotrophic reservoir. The variation of the regression slope of "b" in Z. platypus was accounted for 79.7% [$b=0.012{\times}TSI(TP)+2.395$, p=0.017] by the variation of TSI (TP) and 82.2% [$b=0.013{\times}TSI(Chl-a)+2.36$, p=0.013] by the variation of TSI (Chl-a). The proportion of DELT abnormality increased as the trophic state increases in the reservoirs. The overall data suggest that the growth of the fish populations, based on the length-weight relations and condition factor, reflected the trophic state of nutrient and phytoplankton biomass of the reservoir waters. Thus, in spite of the tolerant characteristics of Z. platypus, hypertrophic states might negatively affect the health of the population.

A study on improvement of misdiagnosis rate in aortic regurgitation disease by physically correcting EF in 2D echo cardiography (대동맥판 역류질환 진단시 이면성심초음파 박출계수의 보정을 통한 오진율 개선에 관한 연구)

  • Choi, Kwan-Woo;Son, Soon-Yong
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.13 no.5
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    • pp.2142-2147
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    • 2012
  • This study is aimed at decreasing wrong diagnosis with corrected EF(Ejection Fraction) of 2D echo cardiography by analysing the physical time-resolution difference between Cine MRI and 2D echo cardiography and applying the corrected EF in 2D echocardiography. From February 2010 to December 2011, among the 110 patients who had undergone both 2D echo cardiography and cine MRI only 37 patient were selected suffering aortic valve regurgitation. ED, ES and SV were measured and EF was calculated in each system while normal ranges of Cine MRI and 2D echocardiography were compared to evauate misdiagnosis rate. The correlation of physical time resolution between 2D echocardiography and MRI was evaluated and the differences were corrected with linear regression coefficient which is derived from linear regression analysis. Blandt-Altman plot was used to evaluate the reliability of corrected 2D echo cardiography EF and compare the error among measured values. The values were compared with MRI normal range and misdiagnosis rate was measured again. As a result, misdiagnosis rates of physical time resolution were measured to be 32.4%(12people) before the correction of EF and 18.9%(7people) after the correction. Also, EF confirmed in Blandt-Altman plot were almost the same with MRI EF. In conclusion, when diagnosing aortic regurgitation disease, simply using 2D echocardiography can easily raise the misdiagnosis rates, therefore considering the MRI machine's physical merits, correcting the time resolution difference is important by calculating time resolution wrong diagnosis would decrease and it is considered to be useful in clinical circumstances.

Characteristics of Electrode Potential and AC Impendance of Perchlorate Ion-Selective Electrodes Based on Quaternary Phosphonium Salts in PVC Membranes (제4급 인산염을 이용한 과염소산 이온선택성 PVC막 전극의 전극전위와 AC 임피던스 특성)

  • 안형환
    • Membrane Journal
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    • v.9 no.4
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    • pp.230-239
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    • 1999
  • Perchlorate ion-selective electrodes in PVC membranes that respond linearly to concentration 106 M were developed by incorporating the quaternary phosphonium salts as a canier. The effects of the chemical structure, the contents of canier, the kind of plasticizer and the membrane thickness on electrode characteristics such as the electrode slope, the linear respone range and the detection limit were studied. With this results, the detectable pH range, selectivity coefficients and AC impedance characteristics were compared and investigated. The perchlorate ion substituents of the quaternary phosphonium salts like tetraoctylphosphonium perchlorate (TOPP) , tetraphenylphosphonium perchlorate(TPPP), and tetrabutylphosphonium perchlorate(TBPP) as a canier were used. The electrode characteristics were better in the ascending order of TBPP < TPPP < TOPP, with the increase of carbon chain length of the alkyl group. Dioctylsebacate(OOS) was best as a plasticizer, the canier contents were better with 11.76 wt% and the optimum membrane thickness was 0.19 mm. Under the above condition, the electrode slope was 56.58 mV/$^P{ClO}_4$,the linear response range was $10^{-1}$\times$10^{-6}$ M, the detection limit was 9.66 x $10^{-7}$ M. The performance of electrode was better than Orion electrode. The electrode potential was stable within the pH range from 3 to 11. The order of the selectivity coefficients for the perchlorate ion was sol < F < Br < 1. With the result of impedance spectrum, it was found that the equivalent circuit for the electrode could be expressed by a series combination of solution resistance, parallel circuit consisting of the double layer capacitance and bulk resistance and Warburg impedance. And solution resistance was almost not appeared and Warburg impedance was highly appeared by diffusion. Then Warburg coefficient was 1.32$\times$$10^74 $\Omega$ $\cdot$ ${cm}^2/s^{1/2}$.

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Lithological Characteristics and Deterioration Diagnosis of Dosolammaaebulsang (Rock-carved Buddha Statue of Dosolam) in the Seonunsa Temple, Gochang, Korea (고창 선운사 도솔암마애불의 암석학적 특성과 손상도 진단)

  • Park, Sung-Mi;Ryu, Keong-Seok;Choi, Hee-Soo;Lee, Chan-Hee
    • Journal of Conservation Science
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    • v.27 no.1
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    • pp.101-114
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    • 2011
  • The rock-carved Buddha statue at Dosolam (Korea Treasure No. 1200) of the Seonunsa temple in Gochang is unique style sculptured on natural rock cliff of 13.0m height. The Buddha statue is composed of volcanic complex with tuff, dacitic tuff breccia, tuff bereccia and lithic tuff. Especially, the Buddha statue is characterized by hydrothermal alteration and fragmentation on the upper and lower part. As a result of damage diagnosis, exfoliation and detachment of physical weathering are high of 11.3% and 9.3%, respectively. Infrared thermography analysis, exfoliation and micro-cracks occurred in the measuring parts that have not been confirmed by naked eyes. Chemical index of alteration and weathering potential index of host rock for the Buddha statue are 55.16 to 64.01 and 6.14 to 9.92 which are represented within highly weathering degree. In surface, dark black, reddish brown and white discoloration are observed prominently in the lower. Brown discoloration 6.9% is highest. According to the P-XRF measurements, high concentration of Fe in common, in part of dark black discoloration was Mn, white and brown discoloration in part of S and Ca content were higher. Biological weathering that yellowish brown and dark gray crustoes lichenes appeared by 20.8% and 13.3%, respectively. Therefore, comprehensive deterioration rate of Buddha statue show physical damage by 21.2%, discoloration for inorganic contaminants by 10.8% and biological damage by 39.4%. Ultrasonic velocity measurement carried out of Buddha statue on the surface by 555 points. Measured value of ultrasonic velocity was about 2,273m/s(1,067 to 3,215m/s, and weathering coefficient is 0.5(0.4 to 0.8) that progress on MW(moderately weathered) to HW(highly weathered) grade of rocks.

Study on the Present Condition and Type Analysis of Rock Korean Chessboards in Korean Landscape (암각장기판의 형상 및 입지 특성에 관한 연구)

  • Jeong, Poo-Reum;Kim, Jeong-Moon;Rho, Jae-Hyun
    • Journal of the Korean Institute of Traditional Landscape Architecture
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    • v.35 no.2
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    • pp.15-25
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    • 2017
  • The objective of this study is to investigate the value of rock Korean chessboards as a corroborative evidence that go, the play culture naturalized from the past, was also performed under the pavilion of the mountain stream, and the old tree. This study executes analysis on the location and form of rock Korean chessboards, physical environment such as the position of surrounding space, the origin of the nomination, surrounding landscape, and historical significance through the research analysis of comprehensive present conditions, and highlights the position types, landscape significance, and preservation value of the rock Korean chessboards of Korea from diverse angles. The result is as follows. 1. The styles of Korean chess (將棋), which identically propagated in the representative countries of Eastern culture, Korea, China, and Japan, and modified in accordance with each country's idea and native tendency, were organized, and confirmed the Korean Go as a naturalized native culture. 2. Out of 15 rock Korean chessboards confirmed through this study, 9 (60%) were categorized as rock Korean chessboards, and 6 (40%) were categorized as stone Korean chessboards. Also, the average size of the go boards were $51cm{\times}46.6cm$, which demonstrated not much difference with present day universal go boards. The Pearson correlation coefficient between bed rock and go boards were 0.647, demonstrating a relatively high correlation; the research subject of rock go boards have been constructed in consideration of rationality, convenience, and the value of promotion of public welfare. 3. The results of analysis of location patterns of rock Korean chessboard showed that villages (87%), mooring (73%), plains (60%) and mountains (47%). The most frequent location pattern is mooring and village, and these two factors played the most important role in determining the location of the rock Korean chessboard.

Changes in Quality of Lettuce During Storage by Immersion-Type Hydrocooling (침지식 냉수냉각에 의한 상치의 저장중 품질변화)

  • Jeong, Jin-Woong;Kim, Byeong-Sam;Kim, Oni-Woung;Nahmgung, Bae;Park, Kee-Jai
    • Korean Journal of Food Science and Technology
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    • v.27 no.4
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    • pp.537-545
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    • 1995
  • Quality changes of lettuce were studied during storage to investigate the efficiency, cooling properties and the washing and storage effects of immersion-type hydrocooling. As a result of plotting the nondimensionalized lettuce temperature versus cooling time, its cooling rate coefficient was shown to be $-0.365\;min^{-1}{\sim}-0.255\;min^{-1}\;(R^2=0.99{\sim}0.88)$. Rate of weight loss was not significantly (p>0.05) different between lettuces with various treatment conditions during storage at $5^{\circ}C$. However, during storage at $15^{\circ}C$, weight loss of hydrocooled lettuce was lower than that of non-treated lettuce after from 10 to 15 days. Especially, The lettuce packed with PE was more effective than that packed with try in terms of packing condition after hydrocooling. Lettuce pretreated with sterilizing agent, packed with PE vinyl film, removed residual water after hydrocooling had lower decaying rate than any other lettuces. Changes of L and b values in hydrocooled lettuce were slower than those of non-treated one. During changes of chlorophyll content, the initial value, $115.7{\sim}147.3\;mg%$ was decreased to $50{\sim}60%$ after 25 days of storage at $5^{\circ}C$ and within 15 days of storage at $15^{\circ}C$. It could be presumed that the addition of sterilizing agent reduced the initial level of overall total and coliform count and its growth rate during storage. The respiration rate of hydrocooled lettuce at $5^{\circ}C$ was $23.95\;mg{\cdot}CO_2/kg\;hr$, which is 10% of those of non-treated lettuce.

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A Study on the Growth Analysis of Pinus koraiensis Seedings under Various Relative Light Intensities and Planting Densities (잣나무 묘목(苗木)의 생육환경(生育環境) 요인(要因)에 따른 생장해석적(生長解析的) 연구(硏究))

  • Kim, Young Chai
    • Journal of Korean Society of Forest Science
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    • v.78 no.3
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    • pp.314-322
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    • 1989
  • For the improvement of nursery condition and the study of eco-physiological characteristics of Pinus koraiensis(2-2) seedlings, the primary productivity was investigated and the growth characteristics and their correlationship were analyzed from May to September in 1987. Seedlings used in this study were grown at the nursery of Experimental Forestry of Kyung Hee Univ. The installation of experimental plots were divided into control plot (100%), 63%, 37% and 19% relative light intensity, and each relative light intensity plots were split into $15{\times}15/m^2$, $12{\times}12/m^2$, $9{\times}9/m^2$ and $6{\times}6/m^2$ plnting density by randomized black design method. To take into account the edge effect of plant population, material were selected from each plot by random sampling at 30 days interval. Each sampled material was divided into leaf and stem drived in a drying oven at the temperature of $85^{\circ}C$ until it had constant weight and weighed. and leaf area was surveyed. Growth analysis for RGR and NAR was done by Blackman method and correlation coefficient were investigated between RGR and NAR by analyzing the dry matter production and growth characteristics of the material, cultivated on the experimental nursery under the condition of different treatment the obtained result were as follows : 1. The increasing rate of dry matter was similar at early stage of growth, but not at late stage. 2. Leaf area growth was the maximum value at 63% relative light intensity and the minimum at 19% RLI 3. The value of RGR was the highest on June under $6{\times}6/m^2$ planting density in 63% relative light intensity, the lowest on September under $12{\times}12/m^2$ of 19% relative light intensity. 4. The change NAR decreased in early stage of growth, while it increased on August and September, The value of RGR on June under $6{\times}6/m^2$ planting density in 100 light intensity was the highest, and on September under $12{\times}12/m^2$ planting density in 19% relative light intensity was the lowest. 5. Regression and correlation between RGR and NAR showed significantly positive.

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